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Wildfire substantially alters aquatic ecosystems by inducing moderate to catastrophic physical and chemical changes. However, the relations of environmental and watershed variables that drive those effects are complex. We present a Driver‐Factor‐Stressor‐Effect (DFSE) conceptual framework to assess the current state of the science related to post‐wildfire water‐quality. We reviewed 64 peer‐reviewed papers using the DFSE framework to identify drivers, factors, stressors, and effects associated with each study. A total of five drivers were identified and ranked according to their frequency of occurrence in the literature: atmospheric processes > fire characteristics > ecologic processes and characteristics > land surface characteristics > soil characteristics. Commonly reported stressors include increased nutrients, runoff, and sediment transport. Furthermore, although several different factors have been used at least once to explain water‐quality effects, relatively few factors outside of precipitation and fire characteristics are frequently studied. We identified several gaps indicating the need for long‐term monitoring, multi‐factor studies, consideration of organic contaminants, consideration of groundwater, and inclusion of soil characteristics. This assessment expands on other reviews and meta‐analyses by exploring causal linkages between influential variables and overall effects in post‐wildfire watersheds. Information gathered from our assessment and the framework itself can be used to inform future monitoring plans and as a guide for modeling efforts focused on better understanding specific processes or to mitigate potential risks of post‐wildfire water quality. Plain Language Summary: Wildfires impact the hydrology and water quality of surface waters that drain burned areas. However, the environmental and watershed factors that drive post‐wildfire water quality are not well understood. We conducted a scoping review of peer‐reviewed publications to assess the current state of the science and identify knowledge gaps. Using the data gathered from the review, we developed a conceptual framework to identify factors that most frequently explain post‐wildfire impacts, the resulting stressors (i.e., changes in the system), and the effects associated with the observed stressors. Precipitation and burn severity are most frequently related to post‐wildfire impacts. Factors such as soil characteristics are studied relatively infrequently. Effects include impaired water quality, increased drinking water treatment, and increased streamflow. Our analysis identified several gaps that can be filled by long‐term monitoring, conducting studies in geographic regions affected by wildfire but where little data exists, expanding current observations to better understand shifts in baseflow contributions, and expanding chemical analyses to include organic contaminants. Key Points: Precipitation and burn severity are studied more often than soil and land characteristics to explain post‐wildfire water qualityIncreased streamflow and impaired water quality are the most documented effects of wildfire, though other effects can be importantKnowledge gaps include long‐term monitoring, multi‐factor studies, and consideration of groundwater and organic contaminants [ABSTRACT FROM AUTHOR]

Introduction In just over four decades since its global emergence, the AIDS epidemic has taken millions of lives. While there have been exceptional advances in antiretroviral therapies, there remains a [...], Interventions to prevent HIV-1 infection and alternative tools in HIV cure therapy remain pressing goals. Recently, numerous broadly neutralizing HIV-1 monoclonal antibodies (bNAbs) have been developed that possess the characteristics necessary for potential prophylactic or therapeutic approaches. However, formulation complexities, especially for multiantibody deliveries, long infusion times, and production issues could limit the use of these bNAbs when deployed, globally affecting their potential application. Here, we describe an approach utilizing synthetic DNA-encoded monoclonal antibodies (dmAbs) for direct in vivo production of prespecified neutralizing activity. We designed 16 different bNAbs as dmAb cassettes and studied their activity in small and large animals. Sera from animals administered dmAbs neutralized multiple HIV-1 isolates with activity similar to that of their parental recombinant mAbs. Delivery of multiple dmAbs to a single animal led to increased neutralization breadth. Two dmAbs, PGDM1400 and PGT121, were advanced into nonhuman primates for study. High peak- circulating levels (between 6 and 34 [micro]g/ml) of these dmAbs were measured, and the sera of all animals displayed broad neutralizing activity. The dmAb approach provides an important local delivery platform for the in vivo generation of HIV-1 bNAbs and for other infectious disease antibodies.

Most of the high-order aberrations of the eye are not circularly symmetric. Hence, while it is well known that human vision is subject to cortically based orientation preference in cell tuning, the optics of the eye might also introduce some orientational anisotropy. We tested this idea by measuring contrast sensitivity at different orientations of sine-wave gratings when viewing through a closed-loop adaptive optics phoropter. Under aberration-corrected conditions, mean contrast sensitivity improved for all observers by a factor of 1.8× to 5×. The detectability of some orientations improved more than others. As expected, this orientation-specific effect varied between individuals. The sensitivity benefits were accurately predicted from MTF model simulations, demonstrating that the observed effects reflected the individual's pattern of high-order aberrations. In one observer, the orientation-specific effects were substantial: an improvement of 8× at one orientation and 2× in another orientation. The experiments confirm that, for conditions that are not diffraction limited, the optics of the eye introduce rotational asymmetry to the luminance distribution on the retina and that this impacts vision, inducing orientational anisotropy. These results suggest that the traditional view of meridional anisotropy having an entirely neural origin may be true for diffraction-limited pupils but that viewing through larger pupils introduces an additional orientation-specific optical component to this phenomenon.

Neural contributions to the age-related reduction in spatial vision are incontrovertible. Whether there are differential age-related changes in the magnocellular (M) and parvocellular (P) pathways across the life span has not been tested extensively. We studied psychophysically the contrast gain signature of the M and P pathways for 13 younger and 13 older observers. Two separate paradigms thought to separate the M and P pathways based on their contrast gain (J. Pokorny & V. C. Smith, 1997) signature were used. A four-square array was presented as an increment or decrement on a background of 115 Td for 35 ms, with one test square presented at a slightly higher or lower retinal illumination. Using a four-alternative forced-choice procedure, the observer's task was to choose the unique square. The two paradigms differed only in the pretrial adaptation and inter-stimulus array. Data were fitted with models of contrast discrimination derived from the unique contrast gain signatures. The fitted models indicate a change in the discrimination functions with age for both the M and P pathways, revealing a shift in the contrast gain slope. Results indicate that both M and P pathways undergo age-related changes, but functional losses appear greater for the P pathway under the conditions tested.

PurposeTo evaluate the influence of spherical aberration on contrast sensitivity using adaptive optics.SettingVision Science and Advanced Retinal Imaging Laboratory, Department of Ophthalmology & Vision Science, University of California, Davis Medical Center, Sacramento, California, USA.MethodsContrast sensitivity at 8 cycles per degree was evaluated using an adaptive optics system that permitted aberrations to be measured with a Hartmann-Shack wavefront sensor and controlled by a 109 actuator continuous-surface deformable mirror that was at a plane conjugate to the observer's pupil. Vertical Gabor patches were viewed through a 6.3 mm diameter pupil conjugate aperture. Contrast sensitivity was measured with the deformable mirror set to produce 1 of 5 spherical aberration profiles (-0.2 to +0.2 microm). Contrast sensitivity over the range of spherical aberration was fitted with a polynomial function.ResultsThree subjects (age 21 to 24 years) participated. The measured total mean spherical aberration resulting from the spherical aberration profiles produced by the deformable mirror was between -0.15 microm and +0.25 microm. The peak contrast sensitivity of this function for the 3 subjects combined occurred at +0.06 microm of spherical aberration. The peak contrast sensitivity was also achieved with positive spherical aberration for each subject's data fitted individually (mean 0.09).ConclusionThere was intersubject variability in the measurements; however, the mean visual performance was best with the introduction of a small positive spherical aberration.

In 2004, the Social Sciences & Humanities Library at the University of California, San Diego (UCSD) implemented use of restaurant-style pagers for checking out Reserves Collection materials. Pagers are given to patrons when a desired reserves item is already in use. When the item is returned, the next patron is paged to the Circulation Desk to pick it up. The authors describe the UCSD Libraries’ process for initially implementing the pagers, explain the success they have seen with the pagers over the past four years, outline other libraries using similar pagers, and offer guidance for other institutions who might consider this innovative and user-friendly service enhancement.

The contributions of optical and neural factors to age-related losses in spatial vision are not fully understood. We used closed-loop adaptive optics to test the visual benefit of correcting monochromatic high-order aberrations (HOAs) on spatial vision for observers ranging in age from 18 to 81 years. Contrast sensitivity was measured monocularly using a two-alternative forced-choice (2AFC) procedure for sinusoidal gratings over 6 mm and 3 mm pupil diameters. Visual acuity was measured using a spatial 4AFC procedure. Over a 6 mm pupil, young observers showed a large benefit of AO at high spatial frequencies, whereas older observers exhibited the greatest benefit at middle spatial frequencies, plus a significantly larger increase in visual acuity. When age-related miosis is controlled, young and old observers exhibited a similar benefit of AO for spatial vision. An increase in HOAs cannot account for the complete senescent decline in spatial vision. These results may indicate a larger role of additional optical factors when the impact of HOAs is removed, but also lend support for the importance of neural factors in age-related changes in spatial vision.