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51. Inflammatory response to nano- and microstructured hydroxyapatite

Author

Mestres, Gemma, Espanol, Montserrat, Xia, Wei, Persson, Cecilia, Ginebra, Maria-Pau, Karlsson Ott, Marjam, Mestres, Gemma, Espanol, Montserrat, Xia, Wei, Persson, Cecilia, Ginebra, Maria-Pau, and Karlsson Ott, Marjam

Abstract

The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal mor- phologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the ef- fect of supplementing the extracts with calcium ions and/or proteins was investigated. Mac- rophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate’s microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate- like crystals substrates compared to the almost non-existent release on needle-like sub- strates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

Published
2015
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54. Bioactive Ceramic and Metallic Surfaces for Bone Engineering (Chapter 12)

Author

Mas-Moruno, Carles, Espanol, Montserrat, Montufar, Edgar-Benjamin, Mestres, Gemma, Aparicio, Conrado, Gil, Francisco-Javier, Ginebra, Maria-Pau, Mas-Moruno, Carles, Espanol, Montserrat, Montufar, Edgar-Benjamin, Mestres, Gemma, Aparicio, Conrado, Gil, Francisco-Javier, and Ginebra, Maria-Pau

Published
2013

57. *Biomimetic Versus Sintered Calcium Phosphates: The In VitroBehavior of Osteoblasts and Mesenchymal Stem Cells

Author

Sadowska, Joanna-Maria, Guillem-Marti, Jordi, Montufar, Edgar Benjamin, Espanol, Montserrat, and Ginebra, Maria-Pau

Abstract

The fabrication of calcium phosphates using biomimetic routes, namely, precipitation processes at body temperature, results in distinct features compared to conventional sintered calcium phosphate ceramics, such as a high specific surface area (SSA) and micro- or nanometric crystal size. The aim of this article is to analyze the effects of these parameters on cell response, focusing on two bone cell types: rat mesenchymal stem cells (rMSCs) and human osteoblastic cells (SaOS-2). Biomimetic calcium-deficient hydroxyapatite (CDHA) was obtained by a low temperature setting reaction, and α-tricalcium phosphate (α-TCP) and β-tricalcium phosphate were subsequently obtained by sintering CDHA either at 1400°C or 1100°C. Sintered stoichiometric hydroxyapatite (HA) was also prepared using ceramic routes. The materials were characterized in terms of SSA, skeletal density, porosity, and pore size distribution. SaOS-2 cells and rMSCs were seeded either directly on the surfaces of the materials or on glass coverslips subsequently placed on top of the materials to expose the cells to the CaP-induced ionic changes in the culture medium, while avoiding any topography-related effects. CDHA produced higher ionic fluctuations in both cell culture media than sintered ceramics, with a strong decrease of calcium and a release of phosphate. Indirect contact cell cultures revealed that both cell types were sensitive to these ionic modifications, resulting in a decrease in proliferation rate, more marked for CDHA, this effect being more pronounced for rMSCs. In direct contact cultures, good cell adhesion was found on all materials, but, while cells were able to proliferate on the sintered calcium phosphates, cell number was significantly reduced with time on biomimetic CDHA, which was associated to a higher percentage of apoptotic cells. Direct contact of the cells with biomimetic CDHA resulted also in a higher alkaline phosphatase activity for both cell types compared to sintered CaPs, indicating a promotion of the osteoblastic phenotype.

Published
2017
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59. Índice de autores

Author

Aceña Díaz, Marta, Amado Guirado, Ester, de Aranzábal Agudo, Maite, Balius Matas, Ramon, Barredo Hernández, María, Batlle Enrich, Cristina, Batlle Vila, Santiago, Bellver Vives, Montse, Bello Miró, Susana, Belloso Rimbau, Jordi, Bras i Marquillas, Josep, Brotons Cuixart, Daniel, Buñuel Álvarez, José Cristóbal, Calvo Terrades, Mar, Campistol Vila<ce:sup loc='post">†</ce:sup>, Josep, Camprodón Rosanas, Ester, Camps i Serra, Francesca, Canosa Úbeda, Pilar, Caravaca Ribera, M.ª Antònia, Caro Carrasco, Israel, Casanovas Gordó, Josep M.ª, Castejón Ponce, Esperanza, Castellví Gil, Antonio, Caubet Busquet, Inmaculada, Cervera i Ral, Pilar, Cid i Xutglà, Inmaculada, Codina Puig, Xavier, Colomer Revuelta, Julia, Córdoba García, Rodrigo, Corretger Rauet, Josep M.ª, Domingo Salvany, Francesc, Duñó Ambròs, Lurdes, Eddy Ives, Lefa S., Esparza Olcina, M.ª Jesús, van Esso Arbolave, Diego L., Estivill Sancho, Eduard, Falgàs Franco, Josep, de la Flor i Brú, Josep Emili, Florensa Palau, Rosa, de Frutos Gallego, Elisa, Fumadó Pérez, Victoria, Sánchez-Ventura, José Galbe, García Sánchez, Núria, García-Alix Pérez, Alfredo, García-Tornel Florensa, Santiago, Gaspà Martí, Josep, González de Dios, Javier, González Trapote, Luis, Guanyabens Calvet, Joan, Guerra Balic, Myriam, Guzmán Molina, Claudia, Hidalgo Vicario, M.ª Inés, Ibáñez Martínez, Núria, Ibáñez Toda, Lourdes, Isnard Blanchar, Rosa M.ª, Jurado Luque, Francisca, Llobet Agulló, M.ª Pilar, Llorens Jové, M.ª Esperanza, Llussà Arboix, Marian, López Cacho, Ferrán, Lora Espinosa, Alfonsa, Luaces Cubells, Carles, Madridejos Mora, Rosa, Marcos Salas, María Victoria, Marès Bermúdez, Josep, Marhuenda Irastorza, Claudia, Martí Fernández, Joan, Martín Espíldora, M.ª Nieves, Martín Ibáñez, Itziar, Martín Martínez, Benjamín, Martínez Roig, Antoni, Masvidal Aliberch, Rosa M.ª, Minguell Martín, Francisco, Mirabent Junyent, Vinyet, Mónaco, Mauricio, Montón Álvarez, José Luis, Muñoz-Santanach, David, de la Cruz, Joy Ngo, Page Glascoe, Frances, Pavía Sesma, Carlos, Pedret Carballido, Carles, Pérez Fabra, Salvador, Pérez Olarte, Pere, Pérez Testor, Carlos, Pérez Testor, Teresa, Pericas Bosch, Joan, Perich Durán, Rosa M.ª, Ramírez Rodríguez, Julio, Rázquin Arias, Marcelo, Ridao i Redondo, Marisa, Rodríguez Alsina, Santiago, Ros i Rahola, Rosa, Vidal-Quadras, Santiago Rosales, Sabrià Pau, Jaume, de Toledo Codina, José Sánchez, Sanz Marcos, Nuria, Sempé, Michel, Sevilla Moya, Juan Carlos, Sibina García, Salvador, Suma Pezzi, Erika, Teodoro Marín, Silvia, Tizón García, Jorge Luis, Torras Colell, Antonio, Torregrosa Bertet, M.ª José, Torrent Español, Montserrat, Traver Cabrera, Pilar, Travería Casanova, Francisco Javier, Trias i Folch, Elisenda, Ugarte Líbano, Ramón, Velasco Manrique, Marta Vega, Vilanova Juanola, José M.ª, Viñas Vidal, Alina, and Vitoria Miñana, Isidro

Published
2013
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60. Ex Vivo Model for Percutaneous Vertebroplasty

Author

Oliveira, Maria Teresa, Lucena, Sonia, Potes, José, Queiroga, Cristina, Rehman, Sarrawat, Dalgarno, Kenneth, Ramos, António, Reis, Joana, Ginebra, Maria-Pau, Canal, Cristina, Espanol, Montserrat, Montufar, Edgar, and Perez, Roman

Subjects
musculoskeletal diseases, ovine, microCT, animal model, biomaterial, percutaneous vertebroplasty
Abstract

The testing of novel biomaterials for percutaneous vertebroplasty depends on suitable animal models. The aim of this study was to develop ex vivo a reproducible and feasible model of percutaneous vertebroplasty, for ulterior application in vivo. A large animal model was used (Merino sheep), due to its translational properties. Vertebroplasty was performed under tactile and fluoroscopic control, through a bilateral modified parapedicular access in lumbar vertebrae (n=12). Care was taken in order to avoid disruption of the vertebral foramen. The average defect volume was 1234±240 mm3. This mean volume ensures practical defects to test novel injectable biomaterials. 6 vertebrae were injected with a commercial cement (Cerament®, Bone Support, Sweden). Adequate defect filling was observed in all vertebrae. All vertebrae were assessed by microCT, prior to and post defect creation and after biomaterial injection. All vertebrae were mechanical tested. No mechanical failure was observed under loads higher than the physiological. Ultimately, this model is considered suitable for pre-clinical in vivo studies, mimicking clinical application.

Published
2015

61. High-aspect-ratio nanostructured hydroxyapatite: towards new functionalities for a classical material.

Author

Diez-Escudero A, Espanol M, and Ginebra MP

Abstract

Hydroxyapatite-based materials have been widely used in countless applications, such as bone regeneration, catalysis, air and water purification or protein separation. Recently, much interest has been given to controlling the aspect ratio of hydroxyapatite crystals from bulk samples. The ability to exert control over the aspect ratio may revolutionize the applications of these materials towards new functional materials. Controlling the shape, size and orientation of HA crystals allows obtaining high aspect ratio structures, improving several key properties of HA materials such as molecule adsorption, ion exchange, catalytic reactions, and even overcoming the well-known brittleness of ceramic materials. Regulating the morphogenesis of HA crystals to form elongated oriented fibres has led to flexible inorganic synthetic sponges, aerogels, membranes, papers, among others, with applications in sustainability, energy and catalysis, and especially in the biomedical field., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Published
2023
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