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92 results on '"Fang, FuGui"'

51. Transcription profiles of oocytes during maturation and embryos during preimplantation development in vivo in the goat

Author

Li, Yunsheng, primary, Sun, Jiangwen, additional, Ling, Yinghui, additional, Ming, Hao, additional, Chen, Zhen, additional, Fang, Fugui, additional, Liu, Ya, additional, Cao, Hongguo, additional, Ding, Jianping, additional, Cao, Zubing, additional, Zhang, Xiaorong, additional, Bondioli, Kenneth, additional, Jiang, Zongliang, additional, and Zhang, Yunhai, additional

Published
2020
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58. The expression and regulation of miR-1 in goat skeletal muscle and satellite cell during muscle growth and development.

Author

Sui, Menghua, Zheng, Qi, Wu, Hao, Zhu, Lu, Ling, Yinghui, Wang, LiJuan, Fang, Fugui, Liu, Ya, Zhang, Zijun, Chu, Mingxing, and Zhang, Yunhai

Subjects
SATELLITE cells, MUSCLE cells, MUSCLE growth, SKELETAL muscle, GOATS, HISTONE deacetylase, MYOCARDIUM
Abstract

MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 was predicted, and the relationship between miR-1 and one of its target genes, histone deacetylase 4 (HDAC4), was analyzed through double luciferase reporter assay, real-time PCR, and western blot. It was found that the miR-1 is most abundantly expressed in goat heart and skeletal muscle tissue. Meanwhile, the expression of miR-1 showed an increasing tendency from new-born goats to the 7-month-old goats, and then its expression decreases as the goats mature further. In addition, the expression levels of miR-1 decreased in goat skeletal muscle satellite cells with the algebraic increasing of cells. At last, the results showed that HDAC4 is a target gene of miR-1 in goat, and miR-1 can inhibit the post-transcriptional expression of HDAC4, but had no significant influence on the mRNA level of HDAC4. It was hypothesized that miR-1 promotes muscle development by inhibiting the post-transcriptional expression of HDAC4 in goat. [ABSTRACT FROM AUTHOR]

Published
2020
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63. Screening and evaluating of long noncoding RNAs in the puberty of goats

Author

Gao, Xiaoxiao, primary, Ye, Jing, additional, Yang, Chen, additional, Zhang, Kaifa, additional, Li, Xiumei, additional, Luo, Lei, additional, Ding, Jianping, additional, Li, Yunsheng, additional, Cao, Hongguo, additional, Ling, Yinghui, additional, Zhang, Xiaorong, additional, Liu, Ya, additional, Fang, Fugui, additional, and Zhang, Yunhai, additional

Published
2017
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65. DNA Methylation Patterns in the Hypothalamus of Female Pubertal Goats

Author

Yang, Chen, primary, Ye, Jing, additional, Li, Xiumei, additional, Gao, Xiaoxiao, additional, Zhang, Kaifa, additional, Luo, Lei, additional, Ding, Jianping, additional, Zhang, Yunhai, additional, Li, Yunsheng, additional, Cao, Hongguo, additional, Ling, Yinghui, additional, Zhang, Xiaorong, additional, Liu, Ya, additional, and Fang, Fugui, additional

Published
2016
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66. Role of Nesfatin-1 in the Reproductive Axis of Male Rat

Author

Gao, Xiaoxiao, primary, Zhang, Kaifa, additional, Song, Min, additional, Li, Xiumei, additional, Luo, Lei, additional, Tian, Yuan, additional, Zhang, Yunhai, additional, Li, Yunsheng, additional, Zhang, Xiaorong, additional, Ling, Yinghui, additional, Fang, Fugui, additional, and Liu, Ya, additional

Published
2016
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70. Effect of estrogen on the expression of GnRH and kisspeptin in the hypothalamus of rats during puberty

Author

Cui, Pei, primary, Yang, Chen, additional, Zhang, Kaifa, additional, Gao, Xiaoxiao, additional, Luo, Lei, additional, Tian, Yuan, additional, Song, Min, additional, Liu, Ya, additional, Zhang, Yunhai, additional, Li, Yunsheng, additional, Zhang, Xiaorong, additional, Su, Shiping, additional, Fang, Fugui, additional, and Ding, Jianping, additional

Published
2015
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75. Reduced Concentration of Androstenone and Up-Regulation of 3β-Hydroxysteroid Dehydrogenase and 17β-Hydroxysteroid Dehydrogenase mRNA Levels by Active Immunisation Against Gonadotropin Releasing Hormone I

Author

Liu, Ya, primary, Fang, Fugui, additional, Jiang, Shudong, additional, Tian, Yuan, additional, Luo, Lei, additional, Song, Min, additional, Su, Shiping, additional, Pu, Yong, additional, Zhou, Jie, additional, and Zhang, Xiaorong, additional

Published
2014
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82. Characterization of Bovine Induced Pluripotent Stem Cells by Lentiviral Transduction of Reprogramming Factor Fusion Proteins

Author

Cao, Hongguo, primary, Yang, Pan, additional, Pu, Yong, additional, Sun, Xueping, additional, Yin, Huiqun, additional, Zhang, Yu, additional, Zhang, Yunhai, additional, Li, Yunsheng, additional, Liu, Ya, additional, Fang, Fugui, additional, Zhang, Zijun, additional, Tao, Yong, additional, and Zhang, Xiaorong, additional

Published
2012
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88. Reduced concentration of androstenone and upregulation of 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase mRNA levels by active immunisation against gonadotropin releasing hormone I.

Author

Liu, Ya, Fang, Fugui, Jiang, Shudong, Tian, Yuan, Luo, Lei, Song, Min, Su, Shiping, Pu, Yong, Zhou, Jie, and Zhang, Xiaorong

Subjects
*ANDROSTENONES, *HYDROXYSTEROID dehydrogenases, *MESSENGER RNA, *IMMUNIZATION, *GONADOTROPIN releasing hormone, *BLOOD serum analysis
Abstract

The aim of this study was to investigate the efficacy of active immunisation against recombinant maltose binding protein-gonadotropin releasing hormone I (MBP-GnRH-I6) in preventing boar taint. The concentrations of testosterone and anti-GnRH-I antibodies in serum were determined by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The mRNA levels of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD) in testes were analysed by real time quantitative PCR. The concentrations of androstenone and 3-methylindole in backfat were assayed by HPLC. Active immunisation against MBP-GnRH-I6 increased the serum concentration of anti-GnRH-I antibodies (P<0.05) and the levels of 3β-HSD and 17β-HSD mRNA in testis. However, it reduced the serum concentrations of testosterone (P<0.05) and androstenone. The concentration of 3-methylindole in backfat (P>0.05) was not changed. Thus, we conclude that vaccination against MBPGnRH- I6 is a practical and effective method to suppress the synthesis of androstenone. [ABSTRACT FROM AUTHOR]

Published
2014
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89. All- trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation.

Author

Pu, Yong, Wang, Zhangfan, Bian, Yani, Zhang, Fei, Yang, Pan, Li, Yunsheng, Zhang, Yunhai, Liu, Ya, Fang, Fugui, Cao, Hongguo, and Zhang, Xiaorong

Subjects
TRETINOIN, GOATS, MAMMAL reproduction, OVUM analysis, APOPTOSIS, METABOLITE analysis
Abstract

All- trans retinoic acid ( t- RA) is a natural component and representative physiologically active metabolite of vitamin A, having multiple physiologic functions. The objective of this study was to evaluate the effect of t- RA on goat oocyte maturation and cumulus cell apoptosis during in vitro maturation ( IVM). Immature goat cumulus-oocyte complexes ( COCs) were matured in vitro in the absence or presence of t- RA at concentrations of 10 nmol/L, 100 nmol/L and 1000 nmol/L. Oocyte maturation and embryo development were assessed by polar body formation and parthenogenetic activation, respectively. Oocyte survival was checked by Trypan blue staining. Apoptosis of cumulus cells was analyzed by terminal deoxynucleotidyl transferase nick end labeling staining and quantitative real-time PCR. In comparison with the control group, 100 nmol/L and 10 nmol/L t- RA significantly improved goat nuclear oocyte maturation and survival ( P < 0.05). Addition of 1000 nmol/L t- RA improved nuclear maturation ( P < 0.05), but had no effect on survival of goat oocytes. t- RA had no positive effect on goat parthenogenetic embryonic cleavage, blastocyst formation or total cell numbers. However, t- RA inhibits the apoptosis of cumulus cells ( P < 0.01). t- RA treatment up-regulated the expression of B-cell lymphoma 2 ( BCL-2), catalase ( CAT) ( P < 0.05) and down-regulated the expression of Caspase-8 ( P < 0.05). In conclusion, t- RA has positive effects on goat oocyte nuclear maturation and reduces apoptotic cumulus cells during IVM. [ABSTRACT FROM AUTHOR]

Published
2014
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90. Role of Ghrelin on Estrogen and Progesterone Secretion in the Adult Rat Ovary During Estrous Cycle.

Author

Fang, Fugui, Wang, Lin, Zhang, Yunhai, Li, Yunsheng, Su, Shiping, and Zhang, Xiaorong

Subjects
*GHRELIN, *ESTROGEN, *PROGESTERONE, *MESSENGER RNA, *LABORATORY rats, *ESTRUS
Abstract

The objective of the present study was to evaluate the effects of ghrelin on the concentrations of estrogen (E2) and progesterone (P4) in serum and the mRNA expression of estrogen receptor beta (ERβ) and progesterone receptor (PRA++B) in ovary in rats during estrous cycle. Adult female Sprague Dawley rats were intracerebroventricularly (i.c.v.) injected with 3 nmol ghrelin during the estrous cycle, and sacrificed 15 min later. Blood samples and ovaries were collected. The concentrations of serum E2 and P4 were measured by radioimmunoassay, while the amount of ERβ and PRA++B mRNA was assessed by real-time quantitative PCR. Our studies showed that ghrelin could significantly reduce the serum concentration of E2 throughout the estrous cycle ( P < 0.05), the serum level of P4 ( P < 0.05), and the amount of ERβ mRNA during metestrus ( P < 0.05). Meanwhile, the amount of PRA++B mRNA was only reduced during diestrus ( P < 0.05). Overall, our present findings provide the first evidence that i.c.v. injection of ghrelin could reduce the serum concentration of E2 and P4 and the level of ERβ and PRA++B mRNA expression, supporting the role of ghrelin in reproduction. [ABSTRACT FROM AUTHOR]

Published
2012
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91. Human exhaled air can efficiently support in vitro maturation of porcine oocytes and subsequent early embryonic development.

Author

Cao Z, Gao D, Xu T, Tong X, Wang Y, Li Y, Fang F, Ding J, Zhang X, and Zhang Y

Abstract

Air phase is an indispensable environmental factor affecting oocyte maturation and early embryo development. Human exhaled air was previously proved to be a reliable and inexpensive atmosphere that sustains normal early development of mouse and bovine embryos. However, whether human exhaled air can support in vitro maturation (IVM) of porcine oocytes is not yet known. To evaluate the feasibility of maturing oocytes in human exhaled air, we examined oocyte morphology, BMP15 expression, nuclear and cytoplasmic maturation. We found that cumulus expansion status, expression levels of BMP15 important for cumulus expansion and the rate of first polar body emission were similar among human exhaled air, 5% O 2 or 20% O 2 in air after IVM of 44 h. Furthermore, the percentage of metaphase II (MII) oocytes showing normal cortical and sub-membranous localization of cortical granules and diffused mitochondrial distribution patterns is comparable among groups. The cleavage, blastocyst rate and total cell number were not apparently different for parthenogenetic activated and somatic cloned embryos derived from MII oocytes matured in three air phases, suggesting oocytes matured in human exhaled air obtain normal developmental competence. Taken together, human exhaled air can efficiently support in vitro maturation of porcine oocytes and subsequent early embryonic development., Competing Interests: Conflicts of Interest
The authors declare no competing or financial interests in this research.

Published
2018
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92. Expression of follicle-stimulating hormone receptor (FSHR), protein kinase B-2 (AKT2) and adapter protein with PH domain, PTB domain, and leucine zipper (APPL1) in pig ovaries.

Author

Zhou N, Wang N, Qin X, Liu Q, Wang J, Dong H, Zhou J, and Fang F

Subjects
Adaptor Proteins, Signal Transducing genetics, Animals, Female, Immunohistochemistry, Proto-Oncogene Proteins c-akt genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, FSH genetics, Tissue Distribution, Adaptor Proteins, Signal Transducing metabolism, Ovary metabolism, Proto-Oncogene Proteins c-akt metabolism, Receptors, FSH metabolism, Swine
Abstract

Follicle-stimulating hormone (FSH) regulates oogenesis and spermatogenesis by binding to its receptor (FSHR) on target cells in the ovary and testis, respectively. The signaling cascades activated after ligand binding are extremely complex and have been shown to include protein kinase A and phosphatidylinositol 3-kinase/protein kinase. The adapter protein APPL1 (adapter protein with PH domain, PTB domain, and leucine zipper), which is an assortment of other signaling proteins, was previously identified to interact with the FSH receptor (FSHR) and the protein kinase B (AKT) pathway. APPL1 plays an important role in promoting cell survival within the preovulatory follicle granulosa layer. Here, we aimed to evaluate the FSHR, AKT2, and APPL1 gene and protein expression levels in the ovaries of different prolific porcine breeds (Wannan Black [WB] and Large White [LW] pigs) using immunohistochemistry and qRT-PCR, respectively. Our results showed that FSHR, AKT2, and APPL1 mRNA levels were significantly higher (P < 0.05) in the ovaries of WB pigs than in the ovaries of LW pigs. Additionally, the FSHR, AKT2, and APPL1 proteins were mainly found distributed in the granulosa cells and oocytes. This study showed that high levels of FSHR, AKT2, and APPL1 were expressed in the ovaries of high prolific breed pigs., (Copyright© by the Polish Academy of Sciences.)

Published
2017
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