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It is widely believed that large composite materials are difficult to build a ship which is longer than 40 meters because of lack of structural stiffness. Up to now, the ships, 72-meter in length, made of glass fiber reinforced composite have been in serve abroad, while 35 meters in China. Therefore, it’s absolutely necessary to study the problem about the stiffness of the 70-metre composite materials hulls. Start from integration design and finite element analysis of composite materials and structure, the influences of material component, laminated angle and laminated sequences of composite materials’ hull on the dynamic stiffness and static stiffness of the ship are studied to explore the method of improving stiffness. The result show that, in the conditions of having the same molded surface, ship's weight and applied stress, it can improve the ship’s longitudinal stiffness to reach standard requirements by using the part of the high modulus fiber as well as the high modulus fiber laying on the outboard of the hull.

Definitions of azimuthal conformal and gnomonic projections were introduced, and contrastive analyses of graticules shapes between these two projections were conducted. Based on theories of perspective azimuthal projection and space geometrical relationships, transformation between azimuthal conformal and gnomonic projections was carried out through series of derivation. Direct transformation formulae between coordinates of the two projections were established with respect to standard parallel. Finally, correctness and reliability of these formulae were tested and verified by numerical examples. The formulae could be widely used and enrich theories of projection transformation to a certain degree, which would offer reference value for charting in polar regions.

Integer least square estimator is known to be optimal in GNSS ambiguity resolution. But it relays on a search in the whole ambiguity space which decreases the computational efficiency. A partial search strategy is proposed in this contribution. The new search is limited in an ambiguity space subset defined by the bootstrapping solution; the search-and-shrink approach is also introduced. Two experiments have shown the new search strategy promotes the computational efficiency a lot without a notable fixing property lost.

A visible-light driven photoelectrochemical (PEC) aptasensor is described that is capable of detecting chloramphenicol (CAP). It is based on the use of a TiO

Background/Aims: Current therapies for spinal cord injury (SCI) have limited efficacy, and identifying a therapeutic target is a pressing need. Sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 2 (SERCA2) plays an important role in regulating calcium homeostasis, which has been shown to inhibit apoptosis. Exendin-4 has been shown to inhibit the apoptosis of nerve cells in SCI, which can also improve SERCA2 expression. In this study, we sought to determine whether exendin-4 plays a protective role in a rat model of SCI via SERCA2. Methods: To investigate the effects of exendin-4 on SCI, a rat model of SCI was induced by a modified version of Allen’s method. Spinal cord tissue sections from rats and western blot analysis were used to examine SERCA2 expression after treatment with the long-acting glucagon-like peptide 1 receptor exendin-4 or the SERCA2 antagonist 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CE). Locomotor function was evaluated using the Basso Beattie Bresnahan locomotor rating scale and slanting board test. Results: Cell apoptosis was increased with CE treatment and decreased with exendin-4 treatment. Upregulation of SERCA2 in female rats with SCI resulted in an improvement of motor function scores and histological changes. Conclusion: These findings suggest that exendin-4 plays a protective role in a rat model of SCI through SERCA2 via inhibition of apoptosis. Existing drugs targeting SERCA2 may be an effective therapeutic strategy for the treatment of SCI.

The present study investigated the effects of vitamin D deficiency on T cell subsets in patients with spinal tuberculosis. In addition, the influence of vitamin D deficiency was investigated on the expression of cytokines IL-1β, IL-6 and TNF-α in intervertebral disc lesions of patients. One hundred and seventeen patients with spinal tuberculosis who received operative treatment in the Department of Orthopedics in Wuhan City Third Hospital from March 2012 to March 2015 were collected. The patients were divided depending upon vitamin D content into the control group (64 cases, vitamin D content 50 nmol/l). Immunofluorescence method was applied to determine the content of T cell subsets in both groups of patients. Intervertebral disc lesion tissues of two groups of patients were obtained during surgery then treated with HE staining and immunohistochemical staining. The values of average optical density obtained under light microscope were observed as the expression quantities of IL-1β, IL-6 and TNF-α, to explore the relationship between vitamin D and the expression of cytokines. When vitamin D is lacking, the expression of T lymphocyte subsets in patients with spinal tuberculosis significantly decreased. Compared with experimental group, the difference was statistically significant (P

A novel method for preparing epoxy graft poly(St-acrylate) composite latex with high graft ratio has been devised. First, esterification was carried out between acrylic acid and an epoxy resin using the monomer as the solvent. The obtained sample, still in solution in the monomer, was emulsified to miniemulsion droplets together with other monomers. Finally, the epoxy graft acrylate composite latex was prepared by miniemulsion copolymerization. This technology will eliminate pollution arising from the organic solvent when solvent-borne systems are used. The enhanced graft ratio was confirmed by FTIR and 1H NMR measurements. The results indicated that the acrylic monomer was grafted onto the epoxy chain. TEM showed the graft composite latex to have a spherical structure with diameter 100 nm. The adhesive performance and anti-corrosive properties on a steel substrate, as measured by salt-spray resistance, were maximized when 4.0% acrylic acid was added to the epoxy resin. Dynamic mechanical analysis (DMA) has been employed to test the damping properties of the composite latex coatings. It was interesting that the tan δ peak values of the coatings were as high as 2.15, clearly exceeding those of general damping materials. Our findings suggest great potential for the composite latex in the field of functional steel coatings.

Summary 1. Root and shoot competition affect plant growth in different ways, but their effects on reproductive allocation have not been investigated. If root and shoot competition affect reproductive output in a population differently, this will influence the evolution of plant populations growing under various competitive regimes. 2. We conducted a field experiment to investigate the effects of root, shoot and full competition from naturally occurring surrounding vegetation on growth and reproduction of an annual plant, Chenopodium acuminatum, under low and high soil fertility. Root competition was eliminated by inserting a PVC pipe vertically into the soil around target individuals, and shoot competition was removed by installing inverted wire cones above-ground. Plants were measured after 11 weeks of growth. The relationships between reproductive (R) and vegetative (V) biomass among treatments were compared. 3. Without fertilizer, the competitive response of target plants to root competition was greater than that to shoot competition, while in the fertilized treatment, the opposite was the case. Fertilization increased target plant size under no or root competition, but did not affect mean plant size for individuals experiencing shoot or full competition. Variation in size among target plants was highest under shoot competition at high fertility. 4. The slope of log R–log V relationship under fertilized conditions was significantly higher than without fertilizer addition. The slope was higher under shoot and full competition than under root or no competition at both fertility levels. There were many more small individuals when competition was for light than for soil resources. These small individuals developed more slowly and had fewer flowering branches and lower reproductive allocation at harvest than large individuals. 5. Synthesis. Our results demonstrated that shoot competition affects the observed pattern of reproductive allometry among individuals in the field, and this has implications for the fitness of competing plants. The steeper log R–log V slope of populations competing above-ground may intensify the role of directional selection under light competition, making the effects of shoot competition more important than those of root competition for the evolution of weeds in fertile environments.

It is a general trend to adopt the geocentric coordinate system as a geodetic datum for the international measurement community. The definition and realization of Chinese geocentric three-dimensional coordinate system (CGCS2000) which has been employed since July 1st, 2008 were introduced in detail. The defining parameters and derived constants of the reference ellipsoid used were given. The comparison between CGCS2000 and WGS84 was carried out. The differences of geodetic coordinates of a point between the two coordinate systems, normal gravity and vertical gradient of normal gravity on the two ellipsoids caused by the change of the flattening of the ellipsoid were analyzed. The results show that these differences could be neglected in view of present measurement accuracies.

This paper works on the vibration control of the space truss structure. The damper made of viscoelastic composite was designed according to the configuration parameters of the truss structure. The parameters of damper were obtained by using the method of modal strain energy. The optimal position configuration of the damper was determined. The truss in the time domain and frequency domain was analyzed. The dynamic characteristics of three structures which are without damper, with damper in the random position configuration and with damper in the optimal position configuration were compared in the sweep excitation and pulse excitation. The result shows that the structure with damper in the optimal position configuration has a great improvement in the amplitude of vibration in the first resonance region and the amplitude attenuation of the truss. The space truss structure with viscoelastic composite damper has excellent vibration control effect.

It is very dangerous for AUV and other vehicles to traverse through the ocean, where there are many barriers. Some technologies such as active sonar and blue-green lasers can not be used to explore these barriers in some special realm, because signal is sent out during the exploring. In order to solve this problem, a new method by using the character of the submarine topography will engender gravity gradient is proposed. An example of calculate the gravity gradient of a subulate topography is given, from the example can find that the gravity gradient aroused by the barrier is very large and can be observed by the gradiometer easily, the result proved the method of this paper is feasible. This study provided a new method for the underwater carrier to avoidance collision, and the method has passive and all-weather characters.

Using GT-POWER to simulate a diesel engines injection timing, calibrating simulation model has been done according to experimental data. Analysis of the effect on engine power characteristics and emission characteristics caused by different pre-injection timing and main injection timing is the main work. The simulation results show that, the output torque and NOx increased when the injection timing is advanced. In contrast, the soot emission is decreased when the injection timing is advanced.

Epoxy graft acrylate composite latex was synthesized by emulsion polymerization using polymerizable emulsifier. The co-polymer and its structure were characterized by FT-IR and TEM, respectively. The latex particles presented a core-shell structure. The water resistance was investigated and displayed a remarkable result. The corrosion resistance and damping property of the composite latex were evaluated by salt spray cabinet and dynamic mechanical analysis (DMA) respectively. The results indicated that the film has excellent anticorrosive and damping property. These proved that the composite latex had a prominent application potential on the field of functional metal coatings.

Based on the application of elliptical vibration cutting method to precision machining of hard and brittle materials and material softening technology through laser heating, a novel composite cutting technique, laser heating and ultrasonic elliptical vibration assisted cutting, is applied to process sintered tungsten carbide. The simulation of the orthogonal cutting process and the effect of frequency and amplitude of vibration and laser heating temperature on cutting force are discussed by using FEA method. Research results have revealed that the main peak of the transient force components increase with the increase of vibration frequency, decrease with the increase of vibration amplitude and laser heating temperature. Moreover, the friction reversal phenomenon is improved with the increase of vibration frequency and amplitude, resulting in the decrease of average cutting force. Compared to common cutting and traditional one-dimensional ultrasonic vibration cutting, the composite cutting technology put forward in this paper has unique cutting force characteristics for such super hard material because of combined action of friction reversal and intermittence cutting for ultrasonic elliptical vibration and material softening for laser heating. The research in the paper has provided a practical reference for the further experiments of laser and ultrasonic assisted cutting.

In order to get the deployable antenna with light weight but large size and high stiffness, this study investigated SMPC self-deployable driver mechanism based on the deformation mechanism of SMPC, and designed the SMPC space deployable antenna. The laminated shell structure with two pieces of back-to-back configuration was analyzed. Finite element analysis revealed that the reasonable central angle of the laminated shell cross-section was 90°. The ends fixing structure of the SMPC hinge was given. The function and structure of the hoop truss deployable antenna were designed to meet the functional and accuracy requirements.

The well-defined block copolymer PMMA-b-PS was prepared by two-step ATRP in emulsion system. GPC results indicate that Mn increased linearly with conversion and polydispersity remained relatively narrow. It presents the characteristics of living polymerization in emulsion system. FT-IR demonstrated that block copolymer PMMA-b-PS could be successfully synthesized by ATRP with macroinitiator PMMA-Cl in emulsion system. The morphological characteristic of the self-assembly depends on the block copolymer concentration and transforms between spheres and rodlike micelles. The property indicates a perfect potential application in drug delivery materials.

The microcrystalline cellulose grafting polymethylmethacrylate (MCC-g-PMMA) copolymer was successfully synthesized by atom transfer radical polymerization (ATRP) using ethanediamine as ligand in Ionic liquid 1-allyl-3-methylimidazolium chloride ([AMIM]Cl). The MCC-g-PMMA was characterized by FT-IR, 1H NMR, SEM and GPC spectroscopies. The results confirmed that the PMMA had been covalently bonded to cellulose backbone. The molecular weight of graft copolymers linearly increased during the polymerization and presented a low polydispersity.

Geomagnetic field information of the vehicle contains abundant positional information, so the positional information can be derived from the geomagnetic observational value. Firstly, base on the analysis of geomagnetic maps of southeast China, the least square algorithm is used for modeling the local geomagnetism on WMM2010(world magnetic model)database. Then, the optimal positional model can be derived. The simulation has been done, from the simulation results we can see that the mean- squared error of the model in latitude and longitude are about 0.7062 sea mile and 1.8735 sea mile respectively. At last, according to the shortcoming of geomagnetic course system such as costing more time and money in calibration error, the partial geomagnetic field model is applied on correcting the compass variation. The analysis of simulation result indicates that the accuracy has been significantly improved.

A series of 2-(1H-pyrazol-1-yl)pyridines are described as inhibitors of ALK5 (TGFβ receptor I kinase). Modeling compounds in the ALK5 kinase domain enabled some optimization of potency via substitutions on the pyrazole core. One of these compounds PF-03671148 gave a dose dependent reduction in TGFβ induced fibrotic gene expression in human fibroblasts. A similar reduction in fibrotic gene expression was observed when PF-03671148 was applied topically in a rat wound repair model. Thus these compounds have potential utility for the prevention of dermal scarring.

A series of linear 2,5-tetraphenylsilole-vinylene-type polymers were successfully synthesized for the first time. The tetraphenylsilole moieties were linked at their 2,5-positions through a vinylene bridge with p-dialkoxybenzenes to obtain polymer PSVB and with 3,6-carbazole to obtain polymer PSVC. For comparison, 2,5-tetraphenylsilole-ethyne-type polymer PSEB was also synthesized, in which the vinylene bridge of PSVB was replaced with an ethyne bridge. Very interestingly, the bridging group (vinylene or ethyne) had a significant effect on the photophysical properties of the corresponding polymers. The fluorescence peak of PSEB at 504 nm in solution originated from the emission of its silole moieties, whereas PSVB and PSVC emitted yellow light and no blueish-green emission from the silole moieties was observed, thus demonstrating that the emissions of PSVB and PSVC were due to their polymer backbones. More importantly, the 2,5-tetraphenylsilole-ethyne polymer exhibited a pronounced aggregation-enhanced emission (AEE) effect but the 2,5-tetraphenylsilole-vinylene polymer was AEE-inactive. Moreover, both AEE-active 2,5-tetraphenylsilole-ethyne polymer and AEE-inactive 2,5-tetraphenylsilole-vinylene polymers were successfully applied as fluorescent chemosensors for the detection of explosive compounds.

The concrete-filled glass fiber reinforced gypsum wall panel is a kind of panel that the inside cavums of the glass fiber hollow gypsum panel are filled with concrete, which can be used as the bearing wall of a building. The influences of eccentricity distance and height to thickness ratio on the bearing capacity of the compression wall panels were studied, and the failure mechanism and bearing capacity of compression wall panels were gained through the experiments of twenty-seven(nine groups) axial compression wall panel specimens and twenty-seven(nine groups) eccentric compression wall panel specimens. The analysis results indicate that the bearing capacity of compression wall panels is obviously affected by the eccentricity distance and height to thickness ratio, and there is a linear relation between bearing capacity and eccentricity distance. The bearing capacity calculation formula of the concrete-filled glass fiber reinforced gypsum wall panel is obtained by regression analysis, which provides reliable gist for structural design of concrete-filled glass fiber reinforced gypsum wall panel buildings.

Rock burst is a geological hazard which occurs when hard and brittle rock mass is excavated under high in-situ stresses or high stress concentration. The Qirehataer diversion tunnel has 15.66 km long at a maximum depth of 1720 m. About 64.5% of the tunnel is located in gneissic granite. During excavation of the tunnel, light to moderate rock burst occurred in about 300 m length of the tunnel. This paper provides the analysis of the characteristics and stress conditions in the rock burst site using the stress conditions around the excavation, and provides an explanation on the reasons why rock burst occurred from the beginning to middle of the vault on the right side of the tunnel section, and the most serious situation is located at 60° with respect to horizontal. Stress release hole and bolt or bolt and net are used to prevent and manage rock burst which have been shown to be effective in this case.

Background Excessive dermal scarring is characterized by an overabundant deposition of extracellular matrix caused by fibrosis. The purpose of this study was to modify a rodent model of cutaneous healing for use in the development of compounds to minimize scarring, and to test the model with a small molecule inhibitor of transforming growth factor-β type I receptor, activin receptor-like kinase 5, because this class of inhibitors has been demonstrated to be effective in minimizing fibrosis in other organs. Methods The rodent model of cutaneous healing consists of uniform full-thickness incisional dermal wounds in rats. Wounds were allowed to heal by secondary intention, generally over a 14-day period. The usefulness of the model was tested by the application of an activin receptor-like kinase 5 inhibitor, CP-639180. Activin receptor-like kinase 5 inhibition antagonizes the transforming growth factor-β pathway, and was used to determine whether there was an effect on collagen deposition in wounds. The compound was applied once per day for 7 days starting at postwounding day 0 or 7 (early or late treatment regimens). Wounds were analyzed histologically for collagen deposition and biochemically for quantification of collagen changes. Results Early and late treatment regimens with the activin receptor-like kinase 5 inhibitor significantly reduced collagen deposition without impairing wound healing. Conclusions Application of a small molecular inhibitor of activin receptor-like kinase 5 appears to significantly reduce collagen deposition in rat dermal wounds as reported here for the first time. Activin receptor-like kinase 5 inhibition may offer a novel approach to reducing proliferative scars in humans because collagen accumulation is a core event in scarring.

Weedy rice represents an important resource for rice improvement. The F(1) hybrid between the japonica wide compatibility rice cultivar 02428 and a weedy rice accession from Yunnan province (SW China) suffered from pollen sterility. Pollen abortion in the hybrid occurred at the early bicellular pollen stage, as a result of mitotic failure in the microspore, although the tapetum developed normally. Genetic mapping in a BC(1)F(1) population (02428//Yunnan weedy rice (YWR)/02428) showed that a major QTL for hybrid pollen sterility (qPS-1) was present on chromosome 1. qPS-1 was fine-mapped to a 110 kb region known to contain the hybrid pollen sterility gene Sa, making it likely that qPS-1 is either identical to, or allelic with Sa. Interestingly, F(1) hybrid indicated that Dular and IR36 were assumed to carry the sterility-neutral allele, Sa ( n ). Re-sequencing SaM and SaF, the two component genes present at Sa, suggested that variation for IR36 and Dular may be responsible for the loss of male sterility, and the qPS-1 sequence might be derived from wild rice or indica cultivars. A phylogenetic analysis based on microsatellite genotyping suggested that the YWR accession is more closely related to wild rice and indica type cultivars than to japonica types. Thus it is probable that the YWR accession evolved from a spontaneous hybrid between wild rice and an ancient cultivated strain of domesticated rice.

A key commonality of most age-related neurodegenerative diseases is the accumulation of aggregation-prone proteins in the brain. Except for the prionoses, the initiation and propagation of these proteopathies in vivo remains poorly understood. In a previous study, we found that the deposition of the amyloidogenic peptide Abeta can be induced by injection of dilute extracts of Alzheimeric neocortex into the brains of Tg2576 transgenic mice overexpressing the human beta-amyloid precursor protein. The present study was undertaken to assess the pathology after long-term (12 months) incubation, and to clarify the distinctive anatomical distribution of seeded Abeta-immunoreactivity. All mice were injected at 3 months of age; 5 months later, as expected, Abeta deposits were concentrated mostly in the injected hemisphere. After 12 months, abundant, transgene-derived Abeta deposits were present bilaterally in the forebrain, but plaque load was still clearly greater in the extract-injected hemisphere. There was also evidence of tau hyperphosphorylation in axons of the corpus callosum that had been injured by the injection, most prominently in transgenic mice, but also, to a lesser degree, in non-transgenic mice. Five months following injection of AD-extract, an isolated cluster of Abeta-immunoreactive microglia was sometimes evident in the ipsilateral entorhinal cortex; the strong innervation of the hippocampus by entorhinal cortical neurons suggests the possible spread of seeded pathology from the injection site via neuronal transport mechanisms. Finally, using India Ink to map the local dispersion of injectate, we found that Abeta induction is especially potent in places where the injectate is sequestered. The AD-seeding model can illuminate the emergence and spread of cerebral beta-amyloidosis and tau hyperphosphorylation, and thus could enhance our understanding of AD and its pathogenic commonalties with other cerebral proteopathies.

Neurofibrillary tangles, one of the pathologic hallmarks of Alzheimer's disease (AD), are composed of abnormally polymerized tau protein. The hyperphosphorylation of tau alters its normal cellular function and is thought to promote the formation of neurofibrillary tangles. Growing evidence suggests that cyclin-dependent kinase 5 (cdk5) plays a role in tau phosphorylation, but the function of the enzyme in tangle formation remains uncertain. In AD, cdk5 is constitutively activated by p25, a highly stable, 25kD protein thought to be increased in the AD brain. To test the hypothesis that p25/cdk5 interactions promote neurofibrillary pathology, we created transgenic mouse lines that overexpress the human p25 protein specifically in neurons. Mice with high transgenic p25 expression have augmented cdk5 activity and develop severe hindlimb semiparalysis and mild forelimb dyskinesia beginning at approximately 3 months of age. Immunohistochemical and ultrastructural analyses showed widespread axonal degeneration with focal accumulation of tau in various regions of the brain and, to a lesser extent, the spinal cord. However, there was no evidence of neurofibrillary tangles in neuronal somata or axons, nor were paired helical filaments evident ultrastructurally. These studies confirm that p25 overexpression can lead to tau abnormalities and axonal degeneration in vivo but do not support the hypothesis that p25-related induction of cdk5 is a primary event in the genesis of neurofibrillary tangles.

The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cell-specific promoter L7/pcp-2. Here, we show that GFPS65T,hu is highly expressed specifically in the cerebellum in whole mount preparations after the 2nd postnatal week. GFPS65T,hu can be detected exclusively in Purkinje cells of cerebellar slices. The fluorescence intensity of GFPS65T,hu should enable the characterization and recording of axons, dendrites, and spines protruding from these neuronal processes. The level of GFP expression can be quantified by western blotting which allows to analyze protein expression and L7/pcp-2 promoter regulation in vivo. The application of cellular and physiological techniques on L7GFP mice will provide a remarkable opportunity to investigate various aspects of neuronal development at the cellular and subcellular levels.

The dynamic fracture problems of the piezoelectric materials with antiplane moving crack are analysed by using function of complex variable in the paper. The results show that the coupled elastic and electric fields inside piezoelectric media depend on the speed of the crack propagation, and have singularity at the crack tip. The stress intensity factor is independent of the speed of the crack propagation, which is identical to the conclusion of purely elasticity. Moreover, independent of the electric loading, the dynamic energy release rate can be expressed by the stress intensity factor and enlarge with the increase of crack speed. High speed of the crack moving could impede the crack growth. At the same time, the crack can be propagated into either curve or bifurcation if the crack speed is higher than the critical speed.

Objective: Human papillomaviruses are closely associated with human cervical cancer, especially HPV types 16 and 18. At present, HPV can not be produced in large quantity; it also has tumorgenicity and these properties of HPV have seriously hampered the development of HPV vaccine. HPV type 16 L1 proteins can assembled into virus-like particles (VLP), which are morphologically identical to the nature virion. In order to develop the recombinant adenovirus vectors of HPV, we constructed a recombinant adenovirus shuttle plasmid pCA14 L1-E7C. Methods: Haman papillomavirus type 16 L1 open reading frame without terminator codon (TAA) (5559-7152) and E7C (682-855) were amplified using PCR. The L1 and E7C fragments were inserted into pGEM-T easy vectors by T-A strategy, named pTAL1 and pTAE7C, pTAL1 was cut with Hind III and BglII, the pTAE7C with BamHI and ClaI. The L1 DNA fragment, E7C and pBluesscript SK were ligated together using T4 DNA ligase, pBSL1-E7C and pBSL1-E7C was digested with Hind III and Xhol. The L1-E7C fragment was inserted into adenovirus shuttle plasmids pCAI4, named pCAI4L1-E7C. DNA sequence results indicated that The L1-E7C DNA fragment can encode the HPV16L1-E7 fusion protein correctly. Results: The L1 and E7C DNA fragments were amplified by PCR and recombinant plasmid pTAL1, pTAE7C, pBSL1-E7C and pCA14L1-E7C were constructed correctly. The pCAi4L1-E7C can be used in the further research work, cotransfected the 293 cell with the parent adenovirus pBHG10. Conclusion: Our results indicated that we have constructed a HPV16L1-E7 fusion DNA fragments and the adenovirus shuttle plasmids pCALI-E7C for the further research.

Although the majority of mRNAs expressed in neurons are confined to the perikaryon, a growing number appear to be transported into dendrites. It is likely that this allows for the local regulation of protein synthesis within discrete subcellular compartments. Here, three different subcellular distribution patterns are demonstrated for four mRNAs that encode proteins highly expressed in Purkinje cells and their dendrites; mRNAs are found in the perikaryon only, perikaryon and proximal dendrite, or perikaryon and proximal plus distal dendrites. Further, it is shown that transport of an mRNA into the dendrites increases its sensitivity to translational inhibition by diphtheria toxin. These data suggest a simple model whereby the transport machinery can regulate the translation of selected mRNAs. Thus, environmental signals that generally affect translational efficiency in concert with the selectivity provided by the transport machinery could provide a means to locally regulate the synthesis of a restricted pool of proteins.

To investigate the effect of compressive stress on the factors for liver regeneration including NF-kappaB, signal transducers and activators of transcription 3 (STATS), c-fos and c-jun in hepatocytes Chang cell line.Human hepatocytes Chang cell line were subjected to compressive stress at 1000 microstain or 2000 microstain, expression of NF-kappaB P65, p-STAT3, c-fos and c-jun were detected by Western blot or RT-PCR at 30 min, 1 h, 2 h, 3 h, 4 h, 6 h after application of compressive stress to indicate the priming of hepatocytes proliferation.Enhanced expressions of NF-kappaB P65 and p-STAT3 were observed in hepatocytes under compressive stress indicated by Western blot, the magnitude of compressive stress loaded significantly affected the level of expression of NF-kappaB P65 at 2 h (P = 0.046) and p-STAT3 at 1 h (P = 0.039), the peak of expression of p-STAT3 was at 30 minutes after stress-loading while NF-kappaB P65 was at 1 hour; RT-PCR showed that expression of c-fos was not significantly different between 1000 microstain and 2000 microstain at each time point, and expression of c-jun was significantly different at 30 minutes (P = 0.026), 1 h (P = 0.031), 2 h (P = 0.033) after compressive stress loading.These results indicate that compressive stress may play an important role in initiating the process of liver regeneration.

Theory of map projections is a branch of cartography studying the ways of projecting the curved surface of the earth and other heavenly bodies into the plane, and it is often called mathematical cartography. There are many fussy symbolic problems to be dealt with in map projections, such as power series expansions of elliptical functions, high order differential of transcendental functions, elliptical integrals and the operation of complex numbers. Many famous cartographers such as Adams (1921), Snyder (1987), Yang (1989, 2000) have made great efforts to solve these problems. Due to historical condition limitation, there were no advanced computer algebra systems at that time, so they had to dispose of these problems by hand, which had often required a paper and a pen. Some derivations and computations were however long and labor intensive such that one gave up midway. Briefly reviewing the existing methods, one will find that these problems were not perfectly and ideally solved yet. Formulas derived by hand often have quite complex and prolix forms, and their orders could not be very high. The most serious problem is that some higher terms of the formulas are erroneous because of the adopted approximate disposal.

To apply the Wright-Giemsa stain in micronucleus test and to explore the stain outcomes of Wright-Giemsa dye of various proportions and staining times.Use Wright-Giemsa dye, Wright dye (staining time 3 min) and Giemsa dye (staining time 5 min) to stain HepG2 and then observe the staining effect. The Wright-Giemsa dye was applied under 5 different proportions (3:1-1:3) and different staining times (1, 3, 5, 10, 15 min).After stained for 3-5 min with the proportion ratio of 3:1 of Wright-Giemsa dye, the HepG2 cells showed much better staining outcomes compared with the single stain of either Wright or Giemsa.Wright-Giemsa stain can be used in cell micronucleus test to obtain good staining outcomes.

Firstly, the limitations of the traditional maximum correlation matching algorithm is analyzed and the new algorithm to approximate local grid gravity anomaly map with the tensor product of Gauss spline function is proposed to get the continuous analytic expression of local gravity anomaly map. Then, the mathematical model of maximum correlation matching algorithm is redesigned based on this unified analytic expression and the BFGS Quasi–Newton method is adopted to solve this non-linear model. With respect to the bad real-time performance of traditional maximum correlation matching algorithm, the way fixing the length of sample sequence is adopted to improve sampling structure and the conversion formula between two rigid transformations centered with different points is derived. Finally, the real-time maximum correlation matching algorithm based on continuous analytic expression of local gravity anomaly map is realized. Two simulations have been done based on 2′ × 2′ gravity anomaly database from satellite altimetry inversion for the comparative analysis. From the simulation results, we can see that the matching results of new algorithm can track the actual path with high precision in case of relatively big observation noise and initial position errors.

The Thomsen-Friedenreich (TF or T) antigen, Galβ1-3GalNAcα1-O-Ser/Thr, is the core 1 structure of O-linked mucin type glycans appearing in tumor-associated glycosylation. The TF antigen occurs in about 90% of human cancer cells and is a potential ligand for the human endogenous galectins. It has been reported that human galectin-1 (Gal-1) and galectin-3 (Gal-3) can perform their cancer-related functions via specifically recognizing TF antigen. However, the detailed binding properties have not been clarified and structurally characterized. In this work, first we identified the distinct TF-binding abilities of Gal-1 and Gal-3. The affinity to TF antigen for Gal-3 is two orders of magnitude higher than that for Gal-1. The structures of Gal-3 carbohydrate recognition domain (CRD) complexed with TF antigen and derivatives, TFN and GM1, were then determined. These structures show a unique Glu-water-Arg-water motif-based mode as previously observed in the mushroom galectin AAL. The observation demonstrates that this recognition mode is commonly adopted by TF-binding galectins, either as endogenous or exogenous ones. The detailed structural comparisons between Gal-1 and Gal-3 CRD and mutagenesis experiments reveal that a pentad residue motif ((51)AHGDA(55)) at the loop (g1-L4) connecting β-strands 4 and 5 of Gal-1 produces a serious steric hindrance for TF binding. This motif is the main structural basis for Gal-1 with the low affinity to TF antigen. These findings provide the intrinsic structural elements for regulating the TF-binding activity of Gal-1 in some special conditions and also show certain target and approach for mediating some tumor-related bioactivities of human galectins.

Pregabalin, a synthetic branched chain γ-amino acid with anticonvulsant, anxiolytic, and analgesic activities, has been shown to bind with high affinity to the voltage-gated calcium channel α(2)δ subunit. Given the broad therapeutic utility of pregabalin, a series of experiments was undertaken to determine the potency, selectivity, and specificity of pregabalin's receptor-binding profile at α(2)δ-1 and α(2)δ-2 subunits of voltage-gated calcium channels along with 38 widely studied receptors and channels. Receptor autoradiography was used to assess regional-binding density of pregabalin throughout the rat spinal cord and brain. In addition, a series of studies using in vivo electrophysiological recordings of γ-aminobutyric acid (GABA)(A)- and GABA(B)-evoked currents was undertaken to determine the interaction of pregabalin with GABAergic receptor subtypes. Together, the results of these studies demonstrate potent and selective binding of pregabalin to α(2)δ-1 and α(2)δ-2 subunits in native and recombinant human and porcine systems. Pregabalin did not interact with any of the 38 receptors and ion channels evaluated, and a variety of central nervous system (CNS)-targeted therapeutic drugs did not show activity at the α(2)δ subunits of voltage-gated calcium channels. Receptor autoradiography demonstrated extensive [(3)H]-pregabalin binding throughout the CNS, with high-level binding in the cortex, hippocampus, cerebellum, dorsal horn of the spinal cord, and amygdala. Finally, receptor-binding and electrophysiological techniques failed to show evidence of an interaction between pregabalin and GABA(A) or GABA(B) receptors. These studies suggest that the clinical effects of pregabalin are likely due to direct and selective interactions with α(2)δ-1 and α(2)δ-2 subunits of voltage-gated calcium channels.

The existing modeling methods for geomagnetism aided inertial navigation and their applicability are analyzed. Due to the requirement of high precision and local application for geomagnetic parameter modeling, a modeling method based on fast Fourier series is introduced to model the local geomagnetic measurements. Then by contrasting research between Fourier fitting modeling and the representative Taylor polynomial modeling, the former has been proved to be of higher fitting precision. The feasibility and effectiveness of the Fourier series model have been showed with the modeling result. At last, from the result of a simulation on a 15′×15′ geomagnetic anomaly data, we can see that the mean square error (MSE) of the Fourier series modeling is less than 0.2283nT. So the modeling problem for regional high precision in geomagnetism aided inertial navigation has been effectively solved.

Some samples of semi-dry flue gas desulfurization (FGD) ash were taken from sinter gas of a steel factory. Scanning electron microscope (SEM) and X-ray diffraction (XRD) analyses were employed to identify the samples in order to investigate their physical and chemical characteristics. The results show that semi-dry FGD ash from a steel factory is stable under atmospheric conditions. It has irregular shape, a smooth surface and loose construction. The size of FGD ash particles is around 0.5—25 µm, the average size is about 5 µm and the median diameter is 4.18 µm. Semi-dry FGD ash from a steel factory consists of CaSO3, CaSO4, CaCO3, some amorphous vitreous material and unburned carbon. An experimental method was found to study the oxidation characteristics of ash. A prediction model of the oxidation efficiency was obtained based on response surface methodology. The results show that not only the temperature, but also gas:solid ratio, play an important role in influencing the oxidation efficiency. The interactions of the gas:solid ratio with temperature play an essential role. An improved response surface model was obtained which can be helpful to describe the degree of oxidation efficiency of semi-dry FGD ash.

Interference and anti-jamming of communication systems are key areas of electromagnetic compatibility research. Because of the restrictions of the complexity of communications systems, it is difficult to accurate analyze system characteristics by model and simulation. This paper presents a hybrid simulation method (a digital behavioral simulation and in-the-loop simulation analysis) to simulate effects of anti-jamming receiver. After achieving an accurate model of receiver, we realized analysis of the additive narrowband interference, additive modulation interference and band selective. Practice proved that the hybrid simulation method is accurate. It can achieve rapid analysis and verification for an unknown communication system.

Some samples of ash were taken from a Semi-dry FGD dealing with sinter gas which was released from iron industry. Scanning electron microscope (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared Spectrometer (FTIR) were employed to identify the samples in order to investigate their physical and chemical characteristics. The result shows that: this kinds of semi-dry FGD ash is stable, and its characteristics have no significant change in the dry environment at room temperature. The contents of CaSO 3 and SO 3 in the semi-dry FGD ash from iron industry are higher than those from power plant gas. The semi-dry FGD ash has smooth surface, loose structure and contain many small holes. It is consist of CaSO 3 , CaSO 4 , CaCO 3 , corundum and mullite, et al. The diameter of the ash is distribute from 3.42 mu m to 13.77 mu m, the mean particle diameter is about 4.18 mu m and specific surface area is about 7.94 m 2 /g.