Search

Your search keyword '"Franceschini, Valeria"' showing total 219 results
Start Over Author "Franceschini, Valeria"
219 results on '"Franceschini, Valeria"'

52. Organizzazione dei sistemi olfattivo e vomeronasale negli ittiopsidi: considerazioni evolutive

Author

FERRANDO S., GALLUS L., GAMBARDELLA C., CANDIANI S., AMAROLI A., VACCHI M., STEVENS J., TAGLIAFIERRO G., VALLARINO M. 72° Congresso dell'Unione Zoologica Italiana, FRANCESCHINI, VALERIA, FERRANDO S., GALLUS L., GAMBARDELLA C., CANDIANI S., AMAROLI A., VACCHI M., STEVENS J., FRANCESCHINI V., TAGLIAFIERRO G., and VALLARINO M. 72° Congresso dell'Unione Zoologica Italiana

Subjects
Sistema olfattivo, Sistema vomeronasale, Ittiopsidi, Evoluzione
Published
2011

53. Inner ear rehabilitation in the deafened cochlea of nod-scid mice following transplantation of human pluripotent mesenchymal stem cells

Author

REVOLTELLA RP, MENINI A, SACCARDI R., FRANCESCHINI, VALERIA, BETTINI, SIMONE, REVOLTELLA RP, FRANCESCHINI V, BETTINI S, MENINI A, and SACCARDI R

Subjects
otorhinolaryngologic diseases, MESENCHYMAL STEM CELLS, INNER EAR, sense organs, REGENERATIVE MEDICINE
Abstract

Objectives. Mesenchymal stem cells (MSC) are currently being investigated in numerous pre-clinical and clinical settings of regenerative medicine. We had previously reported (Revoltella et al., Cell Transplant. 2008; 17, 665-678) that human umbilical cord blood CD133+ stem cells transplanted IV into pre-irradiated nod-scid mice made permanently deaf by ototoxic treatment with kanamycin or intense sound, were able to engraft the cochlea and contribute to inner ear restoration, in vivo. We further investigated here whether human adult MSC derived from either bone marrow or fat (lipid suction), if injected IV to deafened nod-scid mice pre-treated with kanamycin , were able to engraft the damaged cochlea regaining hearing. Materials, Methods & Results. We tested HLA-DQa1 DNA and three human microsatellites (CODIS) as indicators of engrafted cells, finding polymerase chain reaction evidence of chimaerism in various tissues of the host, including the Organ of Corti in the damaged cochlea, at 7, 31 and 60 days following MSC transplant. After 31 days, histology, immunohistochemistry, and lectin staining confirmed the repair process and stimulation ex novo of morphological recovery in the inner ear, contrasting with the lack of morphological repair in control similarly injured but non-transplanted mice. FISH analysis, to detect human genomic sequences from different chromosomes, confirmed persistent engraftment of the regenerating inner ear with a very limited number of chimaeric cells. Dual color FISH analysis provided evidence of a limited positive engraftment in the inner ear and in other mouse tissues, also revealing small numbers of possible heterokaryons, probably resulting from unstable clones derived from fusion of donor with endogenous cells, up to 2 months following transplantation. Stem cells and differentiation pathways focused PCR arrays favoured to select MSC inducing the best response. Conclusions. These findings support the concept that transplanted MSC migrating to the damaged inner ear area may provide conditions for the resumption of a damaged cochlea , emerging as a potential strategy for hearing rehabilitation.

Published
2011

55. Terapia rigenerativa con cellule staminali

Author

FRANCESCHINI, VALERIA, BETTINI, SIMONE, Revoltella R., RICHICHI MATTEO, Franceschini V, Bettini S, and Revoltella R

Subjects
SISTEMA OLFATTIVO, CELLULE STAMINALI
Abstract

L'uomo, pur essendo un animale microsmatico, ha sempre affidato all'olfatto importantissimi compiti per la propria sopravvivenza quali la difesa dai pericoli, la marcatura del territorio, la ricerca del cibo e la riproduzione. L'olfatto costituisce un modello privilegiato per lo studio e la comprensione dei processi molecolari della trasduzione dell'informazione sensoriale laddove la disosmia sembra avere un valore semeiotico forte come sintomo di allarme per molte malattie non solo rinosinusali ma anche neurodegenerative.

Published
2010

77. Human Cord Blood CD133+ Stem Cells Transplanted to Nod-Scid Mice Provide Conditions for Regeneration of Olfactory Neuroepithelium After Permanent Damage Induced by Dichlobenil

Author

Franceschini, Valeria, primary, Bettini, Simone, additional, Pifferi, Simone, additional, Rosellini, Alfredo, additional, Menini, Anna, additional, Saccardi, Ricardo, additional, Ognio, Emanuela, additional, Jeffery, Rosemary, additional, Poulsom, Richard, additional, and Revoltella, Roberto P., additional

Published
2009
Full Text
View/download PDF

79. Cochlear Repair by Transplantation of Human Cord Blood CD133+ Cells to Nod-Scid Mice Made Deaf with Kanamycin and Noise

Author

Revoltella, Roberto P., primary, Papini, Sandra, additional, Rosellini, Alfredo, additional, Michelini, Monica, additional, Franceschini, Valeria, additional, Ciorba, Andrea, additional, Bertolaso, Lucia, additional, Magosso, Sara, additional, Hatzopoulos, Stavros, additional, Lorito, Guiscardo, additional, Giordano, Pietro, additional, Simoni, Edi, additional, Ognio, Emanuela, additional, Cilli, Michele, additional, Saccardi, Riccardo, additional, Urbani, Serena, additional, Jeffery, Rosemary, additional, Poulsom, Richard, additional, and Martini, Alessandro, additional

Published
2008
Full Text
View/download PDF

97. Lectin cytochemical localisation of glycoconjugates in the olfactory system of the lizards Lacerta viridis and Podarcis sicula.

Author

Franceschini, Valeria, Lazzari, Maurizio, and Ciani, Franco

Subjects
LECTINS, IMMUNOGLOBULINS, CYTOCHEMISTRY, GLYCOCONJUGATES, OLFACTORY nerve, LACERTA
Abstract

To investigate the presence of defined carbohydrate moieties on the cell surface of the olfactory and vomeronasal receptor cells and the projections of the latter into the olfactory bulbs, a lectin binding study was performed on the olfactory system of the lizards: Lacerta viridis and Podarcis sicula. Both lizards showed a high lectin binding for N-acetyl-glucosamine in the sensory neurons. The lectin binding patterns in Lacerta indicated that the main olfactory system possessed a moderate density of N-acetyl-galactosamine residues and detectable levels of galactose ones. The vomeronasal system on the other hand contained a high density of N-acetyl-galactosamine moieties and a moderate density of glucosamine ones. In Podarcis the main olfactory system and vomeronasal organ contained respectively detectable and moderate levels of galactose residues. The expression of specific glycoconjugates may be associated with outgrowth, guidance and fasciculation of olfactory and vomeronasal axons. [ABSTRACT FROM AUTHOR]

Published
2000
Full Text
View/download PDF

100. Glial fibrillary acidic protein and vimentin immunoreactivity of astroglial cells in the central nervous system of adult Podarcis sicula(Squamata, Lacertidae)

Author

LAZZARI, MAURIZIO and FRANCESCHINI, VALERIA

Abstract

The present immunoperoxidase cytochemical study describes the distribution of glial intermediate filament molecular markers, glial fibrillary acidic protein (GFAP) and vimentin, in the brain and spinal cord of the adult lizard, Podarcis sicula. GFAP immunoreactivity is abundant and the positive structures are mainly represented by fibres of different lengths which are arranged in a rather regular radial pattern throughout the CNS. They emerge from generally immunopositive radial ependymoglia and are directed from the ventricular wall towards the meningeal surface. The glial fibres give origin to endfeet which are apposed to the blood vessel walls and subpial surface where they form the continous perivascular and subpial glia envelopes, respectively. In the optic tectum and spinal cord, star‐shaped astrocytes coexist with radial glia. In the spinal cord, cell bodies of immunopositive radial glia are displaced from the ependyma. While vimentin immunoreactive elements are almost completely absent in the brain except for a few diencephalic radial fibres, the spinal cord ependyma exhibits a clearly vimentin positivity and no GFAP staining. In the PodarcisCNS the immunocytochemical response of the astroglial intermediate filaments appears typical of mature astroglia cell lineage since it fundamentally expresses GFAP immunoreactivity. Moreover, this immunocytochemical study shows that the Podarcisfibre pattern with predominant radial glial cells is morphologically more immature than in avians and mammalians, a condition suggesting that reptiles represent a fundamental step in the phylogenetic evolution of vertebrate astroglial cells.

Published
2001
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results