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Additional file 2: Figure S1. Relative abundance (%) of the major genera present in the fungal microbial communities. Figure S2. Comparison of microbial composition in different environments based on SVM. Figure S3. Comparison of the diversity of the Penicillium between the different crew groups. Figure S4. Box and whisker plots of the Shannon diversity index of the HTS dataset according to the day of isolation (time). Figure S5. Trophic modes of the fungal microbiome in the different groups (G1 and G2). Figure S6. Comparison of community diversity among different occupant groups and different sampling locations. Figure S7. Trophic modes of the fungal microbiome in the different locations (CC, PC and SC). Figure S8. Comparison of the total expression levels of mycotoxin genes among different occupant groups. Figure S9. Comparison of fungal toxin gene expression at different locations. Figure S10. Correlation network analysis of the fungal community.

Additional file 2: Figure S1. (a) Comparison of microbial composition in different environments, according to SVM analysis. (b) Relative abundance (%) of the major genera present in the fungal microbial communities. LP: Lunar Palace 1; ISS: International Space Station. IP: indoor with plants. CO: classroom outdoor. CR: university classroom. Figure S2. Significant differences in richness diversity estimates of the microbial communities between the two crews. Figure S3. Relative sequence abundance, based on amplicon sequencing, of bacterial genera associated with G1 and G2. Figure S4. Relative sequence abundance, based on metagenome sequencing, of bacterial phyla (a) and genera (b) associated with G1 and G2. Figure S5. Comparison of community diversity among the different occupant groups and different sampling locations. (a) Comparison of community diversity among the different occupant groups in the CC. (b) Comparison of community diversity among the different occupant groups in the PC. (c) Comparison of community diversity among the different occupant groups in the SC. (d) Comparison of community diversity among the different sampling sites during the G1 group. (e) Comparison of community diversity among the different sampling sites during the G2 group. CC, comprehensive cabin; PC, plant cabin; SC, solid waste treatment cabin. Figure S6. Microbial functional diversity estimates for the two shifts. (a) Functional diversity between the two crew groups (no significant difference). (b) Functional redundancy index. (c) Functional diversity analysis based on metagenomics between the two crew groups. Figure S7. Correlation network analysis between ARGs and the airborne microbiome (P 0.4). Grey lines between the nodes indicate positive connections between the genera, while red lines indicate negative correlations.

Aim: Our aim in this study was to isolate potentially novel strains of fowl adenovirus serotype-4 (FAdV-4) that is currently circulating in broiler chicken flocks in Guangdong Province, China, and to compare nucleotide and amino acid (AA) sequences of their respective hexon genes. Materials and Methods: The experiment was carried out on poultry farms experiencing outbreaks of FAdV-4-associated hydropericardium syndrome (HPS). Tissue samples from the hearts and livers of deceased chickens were screened for FAdV-4 infection using hexon gene-specific polymerase chain reaction (PCR). Results: New virus isolates were used to infect 7-day-old chicks, which went onto reproduce typical HPS signs. The hypervariable region of the FAdV-4 hexon gene was PCR-amplified and sequenced. The hexon nucleotide and deduced AA sequence identities were 99.8-99.9% and 99.5-99.8%, respectively, among the four novel isolates. In addition, the new isolates were 97-100% and 96.4-99.9% identical to the nucleotide and deduced AA sequences, respectively, of FAdV-4 hexon genes available in the National Center for Biotechnology Information GenBank database. Phylogenetic analyses, based on the hexon gene sequence, revealed that the new isolates, clustered with FAdV-C; the FAdV-A, FAdV-B, FAdV-D, and FAdV-E viruses, were more distantly related. Conclusion: New FAdV-4 isolates from Guangdong Province are similar to those identified in other regions of the world. This information provides critical insight into HPS epidemiology and provides a perspective for monitoring outbreaks and developing strategies for disease prevention.