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Gray mold, caused by Botrytis sp., is a significant disease in Colombian rose crops and its control depends primarily on the intensive use of chemically synthesized fungicides. Despite the importance of this pathogen, there is limited information in Colombian floriculture about molecular taxonomy of species, fungicide resistance of populations and their genetic mechanism of resistance. In this study, we analyze 12 isolates of this fungus collected from rose-producing crops in the Department of Cundinamarca and conducted phylogenetic analysis using HSP60, G3PDH, and RPB2 gene sequences. Additionally, we realize phenotypic and genotypic characterization of resistance to the fungicides fenhexamid, carboxin, and prochloraz, evaluating the in vitro EC50 and presence of mutations of target genes of each isolate. All isolates were characterized as Botrytis cinerea in the phylogenetic analysis and presents different levels of resistance to each fungicide. These levels are related to mutations in target genes, with predominancy of L195F and L400F in the ERG27 gene to fenhexamid resistance, H272R/Y in the SDHB gene for carboxin resistance, and Y136F in the CYP51 gene for prochloraz resistance. Finally, these mutations were not related to morphological changes. Collectively, this knowledge, presented for the first time to the Colombian floriculture, contribute to a better understanding of the genetic diversity and population of B. cinerea from rose-producing crops in the department of Cundinamarca, and serve as a valuable tool for making informed decisions regarding disease management, future research, and improving crop management and sustainability in the Colombian floriculture industry. [ABSTRACT FROM AUTHOR]

The runoff threshold is the amount of precipitation that exceeds the infiltration capacity of the soil and from which water begins to flow over the land surface. Its spatial variation will determine the appearance of a series of processes and risks such as soil erosion, sediment transport, water pollution or flooding, all of which are of great interest in the field of land planning and management. This article provides an update of the runoff threshold in the territorial scope of the Demarcación Hidrográfica de las Cuencas Mediterráneas Andaluzas and evaluates the spatial changes occurred between 1990 and 2018 because of land use dynamics. For this purpose, the methodology described in Regulation 5.2-IC related to surface drainage, of the Instrucción de Carreteras (Ministerio de Obras Pu'blicas y Urbanismo [MOPU], 1990; Ministerio de Fomento, 2016, 2019) was used. The results obtained show very contrasting runoff threshold values, with maximums in the western sector and minimums in the eastern sector. On the other hand, the dynamics of land use changes have led to a significant increase in impermeable surfaces and sclerophyllous vegetation, which has generated a clear reduction in runoff threshold values in different areas, mainly in the eastern region. Specifically, the former forest areas, currently occupied by sclerophyllous vegetation, stand out, with a reduction of 17 mm over an area of approximately 229.1 km2. The conversion of agricultural lands into urban areas is also noteworthy, with a decrease in their retention capacity by up to 14 mm of precipitation, covering a total area of 97.1 km2. [ABSTRACT FROM AUTHOR]

Objective: To analyze the state-of-the-art technology and the application of the fermentation process, in order to increase the protein value of prickly pear (Opuntia ficus-indica), as a source of fodder, in three technological levels. Design/Methodology/Approach: The technological parameters and mechanization level of three fermenters —employed to increase the protein value of prickly pear fodder— were characterized. The conceptual technical analysis was determined through quantitative and qualitative indicators, based on mathematical expressions and equations. Results: Quantitative indicators showed a fermentation technology with limited efficiency, which provides an opportunity for technical improvements. Findings/Conclusions: The application of fermentation technology results in significant savings in water, soil, and fertilizer resources. Furthermore, it provides an alternative for fodder production and food security for livestock in the semi-arid region of Mexico. [ABSTRACT FROM AUTHOR]

Background: Stress urinary incontinence (SUI) is a common condition with a significant impact on the quality of life of female patients. The limitations of current treatment strategies have prompted the exploration of new effective and minimally invasive alternative approaches, including cell therapy. Methods: A literature search was conducted to update the current clinical status of stem cell therapy in the management of female stress urinary incontinence. Results: Over thirty clinical studies have been designed to assess the feasibility, safety and efficacy of cell therapy for female SUI. Despite differences in cell types and protocols, the overall treatment procedures were similar. Standard subjective and objective assessment tools, and follow-up periods ranged from 6 weeks to 6 years have been used. Cell injection has shown to be a safe therapy in the treatment of female SUI. However, the results from more recent randomized trials have shown less promising results than expected in restoring continence. Heterogeneous research methodologies using different cell types and doses make it difficult to draw conclusions about effectiveness. Several key points remain that need to be further explored in future clinical trials. Conclusion: To advance in the development of cell therapy, it is essential to know the mechanisms involved to be able to direct it properly, its efficacy and the durability of the injected cells. Rigorous and homogenized preclinical and clinical studies that demonstrate its scope and improve its application are necessary for validation in the treatment of female SUI. [ABSTRACT FROM AUTHOR]

In this study, the resistive switching phenomena in TiN/Ti/HfO2/Ti metal–insulator–metal stacks is investigated, mainly focusing on the analysis of set and reset transitions. The electrical measurements in a wide temperature range reveal that the switching transitions require less voltage (and thus, less energy) as temperature rises, with the reset process being much more temperature sensitive. The main conduction mechanism in both resistance states is Space-charge-limited Conduction, but the high conductivity state also shows Schottky emission, explaining its temperature dependence. Moreover, the temporal evolution of these transitions reveals clear differences between them, as their current transient response is completely different. While the set is sudden, the reset process development is clearly non-linear, closely resembling a sigmoid function. This asymmetry between switching processes is of extreme importance in the manipulation and control of the multi-level characteristics and has clear implications in the possible applications of resistive switching devices in neuromorphic computing. [ABSTRACT FROM AUTHOR]