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The Siegert relation relates field and intensity temporal correlations. After a historical review of the Siegert relation and the Hanbury Brown and Twiss effect, we discuss the validity of this relation in two different domains. We first show that this relation can be used in astrophysics to determine the fundamental parameters of stars, and that it is especially important for the observation with stellar emission lines. Second, we check the validity of this relation for moving quantum scatterers illuminated by a strong driving field., (© The Author(s) 2022.)

Superradiance has been extensively studied in the 1970s and 1980s in the regime of superfluorescence, where a large number of atoms are initially excited. Cooperative scattering in the linear-optics regime, or "single-photon superradiance," has been investigated much more recently, and superradiant decay has also been predicted, even for a spherical sample of large extent and low density, where the distance between atoms is much larger than the wavelength. Here, we demonstrate this effect experimentally by directly measuring the decay rate of the off-axis fluorescence of a large and dilute cloud of cold rubidium atoms after the sudden switch off of a low-intensity laser driving the atomic transition. We show that, at large detuning, the decay rate increases with the on-resonance optical depth. In contrast to forward scattering, the superradiant decay of off-axis fluorescence is suppressed near resonance due to attenuation and multiple-scattering effects.

Background: Written comments by medical student supervisors provide written foundation for grade narratives and deans' letters and play an important role in student's professional development. Written comments are widely used but little has been published about the quality of written comments. We hypothesized that medical students share an understanding of qualities inherent to a high-quality and a low-quality narrative comment and we aimed to determine the features that define high- and low-quality comments., Methods: Using the well-established anthropological pile-sort method, medical students sorted written comments into 'helpful' and 'unhelpful' piles, then were interviewed to determine how they evaluated comments. We used multidimensional scaling and cluster analysis to analyze data, revealing how written comments were sorted across student participants. We calculated the degree of shared knowledge to determine the level of internal validity in the data. We transcribed and coded data elicited during the structured interview to contextualize the student's answers. Length of comment was compared using one-way analysis of variance; valence and frequency comments were thought of as helpful were analyzed by chi-square., Results: Analysis of written comments revealed four distinct clusters. Cluster A comments reinforced good behaviors or gave constructive criticism for how changes could be made. Cluster B comments exhorted students to continue non-specific behaviors already exhibited. Cluster C comments used grading rubric terms without giving student-specific examples. Cluster D comments used sentence fragments lacking verbs and punctuation. Student data exhibited a strong fit to the consensus model, demonstrating that medical students share a robust model of attributes of helpful and unhelpful comments. There was no correlation between valence of comment and perceived helpfulness., Conclusions: Students find comments demonstrating knowledge of the student and providing specific examples of appropriate behavior to be reinforced or inappropriate behavior to be eliminated helpful, and comments that are non-actionable and non-specific to be least helpful. Our research and analysis allow us to make recommendations helpful for faculty development around written feedback.

Since Dicke's seminal paper on coherence in spontaneous radiation by atomic ensembles, superradiance has been extensively studied. Subradiance, on the contrary, has remained elusive, mainly because subradiant states are weakly coupled to the environment and are very sensitive to nonradiative decoherence processes. Here, we report the experimental observation of subradiance in an extended and dilute cold-atom sample containing a large number of particles. We use a far detuned laser to avoid multiple scattering and observe the temporal decay after a sudden switch-off of the laser beam. After the fast decay of most of the fluorescence, we detect a very slow decay, with time constants as long as 100 times the natural lifetime of the excited state of individual atoms. This subradiant time constant scales linearly with the cooperativity parameter, corresponding to the on-resonance optical depth of the sample, and is independent of the laser detuning, as expected from a coupled-dipole model.

We experimentally investigate the Bragg reflection of light at one-dimensionally ordered atomic structures by using cold atoms trapped in a laser standing wave. By a fine-tuning of the periodicity, we reach the regime of multiple reflection due to the refractive index contrast between layers, yielding an unprecedented high reflectance efficiency of 80%. This result is explained by the occurrence of a photonic band gap in such systems, in accordance with previous predictions.

We address the problem of achieving a random laser with a cloud of cold atoms, in which gain and scattering are provided by the same atoms. In this system, the elastic scattering cross-section is related to the complex atomic polarizability. As a consequence, the random laser threshold is expressed as a function of this polarizability, which can be fully determined by spectroscopic measurements. We apply this idea to experimentally evaluate the threshold of a random laser based on Raman gain between non-degenerate Zeeman states and find a critical optical thickness on the order of 200, which is within reach of state-of-the-art cold-atom experiments.

We address the problem of achieving an optical random laser with a cloud of cold atoms, in which gain and scattering are provided by the same atoms. The lasing threshold can be defined using the on-resonance optical thickness b0 as a single critical parameter. We predict the threshold quantitatively, as well as power and frequency of the emitted light, using two different light transport models and the atomic polarizability of a strongly pumped two-level atom. We find a critical b0 on the order of 300, which is within reach of state-of-the-art cold-atom experiments. Interestingly, we find that random lasing can already occur in a regime of relatively low scattering.

We realize a laser with a cloud of cold rubidium atoms as gain medium, placed in a low-finesse cavity. Three different regimes of laser emission are observed corresponding, respectively, to Mollow, Raman, and four-wave mixing mechanisms. We measure an output power of up to 300 microW and present the main properties of these different lasers in each regime.

We have cloned and characterized novel oxygenolytic ortho-dehalogenation (ohb) genes from 2-chlorobenzoate (2-CBA)- and 2,4-dichlorobenzoate (2,4-dCBA)-degrading Pseudomonas aeruginosa 142. Among 3,700 Escherichia coli recombinants, two clones, DH5alphaF'(pOD22) and DH5alphaF'(pOD33), converted 2-CBA to catechol and 2,4-dCBA and 2,5-dCBA to 4-chlorocatechol. A subclone of pOD33, plasmid pE43, containing the 3,687-bp minimized ohb DNA region conferred to P. putida PB2440 the ability to grow on 2-CBA as a sole carbon source. Strain PB2440(pE43) also oxidized but did not grow on 2,4-dCBA, 2,5-dCBA, or 2,6-dCBA. Terminal oxidoreductase ISPOHB structural genes ohbA and ohbB, which encode polypeptides with molecular masses of 20,253 Da (beta-ISP) and 48,243 Da (alpha-ISP), respectively, were identified; these proteins are in accord with the 22- and 48-kDa (as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) polypeptides synthesized in E. coli and P. aeruginosa parental strain 142. The ortho-halobenzoate 1,2-dioxygenase activity was manifested in the absence of ferredoxin and reductase genes, suggesting that the ISPOHB utilized electron transfer components provided by the heterologous hosts. ISPOHB formed a new phylogenetic cluster that includes aromatic oxygenases featuring atypical structural-functional organization and is distant from the other members of the family of primary aromatic oxygenases. A putative IclR-type regulatory gene (ohbR) was located upstream of the ohbAB genes. An open reading frame (ohbC) of unknown function that overlaps lengthwise with ohbB but is transcribed in the opposite direction was found. The ohbC gene codes for a 48,969-Da polypeptide, in accord with the 49-kDa protein detected in E. coli. The ohb genes are flanked by an IS1396-like sequence containing a putative gene for a 39,715-Da transposase A (tnpA) at positions 4731 to 5747 and a putative gene for a 45,247-Da DNA topoisomerase I/III (top) at positions 346 to 1563. The ohb DNA region is bordered by 14-bp imperfect inverted repeats at positions 56 to 69 and 5984 to 5997.

Under anaerobic conditions and in the absence of alternative electron acceptors, growth of the magnetic bacterium Aquaspirillum magnetotacticum MSI was iron concentration dependent. Weak chelation of the iron (with quinate, oxalate, or 2,3-dihydroxybenzoate) enhanced growth, whereas strong chelation (with EDTA, citrate, or nitrilotriacetic acid) retarded the growth of strain MSI relative to that of controls lacking chelators. Growth was proportional to the percentage of unchelated iron in medium containing EDTA in various molar ratios to iron. Addition of the respiratory inhibitors antimycin A (5 muM), NaCN (10 mM), and NaN(3) (10 mM) inhibited growth with Fe(III) or NO(3) as the terminal electron acceptor. Growth with O(2) and NO(3) was inhibited by 2-heptyl-4-hydroxyquinolone-N-oxide (HOQNO) but not with 2 mM Fe(III). Under strongly reducing conditions, strain MS1 survived but grew poorly and became irreversibly nonmagnetic. Growth and iron reduction in anaerobic cultures were stimulated by the provision of small amounts of O(2) or H(2)O(2). Slow infusion of air to cultures which had reduced virtually all of the Fe(III) in the medium (2 mM) supported a high rate of iron reoxidation (relative to killed controls) and growth in proportion to the amount of iron reoxidized. Oxygen consumption by iron-reducing cultures was predominantly biological, since NaCN and HOQNO both inhibited consumption. Inhibition of oxygen consumption (and iron reoxidation) by the addition of ferrozine and the inhibition of iron oxidation (and oxygen consumption) by the addition of HOQNO suggest that iron oxidation by strain MS1 is an aerobic respiratory process, perhaps tied to energy conservation. Iron oxidation was also necessary for magnetite synthesis, since in microaerobic denitrifying cultures, sequestration of reduced iron by ferrozine present in 10-fold molar excess to the available iron resulted in loss of magnetism and a severe drop in the average magnetosome number of the cells.

The polycyclic aromatic hydrocarbon phenanthrene was mineralized in two stages by soil, estuarine water, and sediment microbial populations. At high concentrations, phenanthrene was degraded, with the concomitant production of biomass and accumulation of Folin-Ciocalteau-reactive aromatic intermediates. Subsequent consumption of these intermediates resulted in a secondary increase in biomass. Analysis of intermediates by high-performance liquid chromatography, thin-layer chromatography, and UV absorption spectrometry showed 1-hydroxy-2-naphthoic acid (1H2NA) to be the predominant product. A less pronounced two-stage mineralization pattern was also observed by monitoring CO(2) production from low concentrations (0.5 mg liter) of radiolabeled phenanthrene. Here, mineralization of C-labeled 1H2NA could explain the incremental CO(2) produced during the later part of the incubations. Accumulation of 1H2NA by isolates obtained from enrichments was dependent on the initial phenanthrene concentration. The production of metabolites during polycyclic aromatic hydrocarbon biodegradation is discussed with regard to its possible adaptive significance and its methodological implications.

Paired surface microlayer and bulk water samples from five sites in the Great Bay Estuary, New Hampshire, were examined with regard to numbers of bacteria,(14)C-phenanthrene biodegradation potentials, and organic and inorganic chemical characteristics. Microlayer samples were generally enriched in nutrients (N and P), dissolved organic matter, and culturable heterotrophic bacteria compared with their corresponding bulk waters. Microlayer samples from marina environments were also enriched in aromatic hydrocarbons, as determined by UV spectrophotometric and fluorometric analyses, and demonstrated substantial phenanthrene biodegradation activity in the assay employed. Biodegradation activity of marina bulk water samples ranged from nil to levels exceeding those exhibited by microlayer samples. No diminution of biodegradation activity was observed after filtration (1.2 μm effective retention) of microlayer water, indicating that the responsible organisms were not particle-associated. Phenanthrene-degrading bacteria, enumerated by counting clearing zones in a crystalline phenanthrene overlay after colony development on a phenanthrene/toluene agar (PTA) medium, were superior to epifluorescence direct counts or standard plate counts on PTA or estuarine nutrient agar in predicting(14)C-phenanthrene biodegradative activity.