63 results on '"Gurates, Bilgin"'
Search Results
52. Wilms’ tumor 1 inhibits aromatase P450 expression in human cells and is downregulated in endometriosis vs. endometrium-derived stromal cells
- Author
-
Gurates, Bilgin, primary, Sebastian, Siby, additional, Yang, Sijun, additional, Tamura, Mitsutoshi, additional, Fang, Zong Juan, additional, and Bulun, Serdar E, additional
- Published
- 2002
- Full Text
- View/download PDF
53. Up-regulation of Cyclooxygenase-2 Expression and Prostaglandin Synthesis in Endometrial Stromal Cells by Malignant Endometrial Epithelial Cells
- Author
-
Tamura, Mitsutoshi, primary, Sebastian, Siby, additional, Yang, Sijun, additional, Gurates, Bilgin, additional, Ferrer, Karen, additional, Sasano, Hironobu, additional, Okamura, Kunihiro, additional, and Bulun, Serdar E., additional
- Published
- 2002
- Full Text
- View/download PDF
54. Vascular Endothelial Growth Factor Up-Regulates Cyclooxygenase-2 Expression in Human Endothelial Cells
- Author
-
Tamura, Mitsutoshi, primary, Sebastian, Siby, additional, Gurates, Bilgin, additional, Yang, Sijun, additional, Fang, Zongjuan, additional, and Bulun, Serdar E., additional
- Published
- 2002
- Full Text
- View/download PDF
55. Role of aromatase in endometrial disease
- Author
-
Bulun, Serdar E., primary, Yang, Sijun, additional, Fang, Zongjuan, additional, Gurates, Bilgin, additional, Tamura, Mitsutoshi, additional, Zhou, Jianfeng, additional, and Sebastian, Siby, additional
- Published
- 2001
- Full Text
- View/download PDF
56. Presacral Solitary Giant Neurofibroma Without Neurofibromatosis Type 1 Presenting as Pelvic Mass. Case Report.
- Author
-
TOPSAKAL, Cahide, primary, EROL, Fatih Serhat, additional, OZERCAN, Ibrahim, additional, MURAT, Ayse, additional, and GURATES, Bilgin, additional
- Published
- 2001
- Full Text
- View/download PDF
57. Ghrelin expression of endometrium hyperplasia and endometrioid carcinoma.
- Author
-
Dagli, Adile F., Aydin, Suleyman, Kocdor, Hilal, Gurates, Bilgin, Sahin, İbrahim, Catak, Zekiye, Ozercan, Mehmet R., and Ozercan, İbrahim. H.
- Subjects
GHRELIN ,HYPERPLASIA ,ENDOMETRIAL cancer ,CANCER in women ,PAROTID glands ,DIAGNOSIS - Abstract
Background. Endometrium carcinoma ranks fourth among female carcinomas. Therefore, early diagnosis of endometrium pre-malignant lesions is emphasised, and attempts are made to identify the risk factors. Since hyperplasias, particularly those with atypia, are held responsible for the development of the most common endometrium carcinomas, it is important to definitely distinguish between well-differentiated carcinomas and hyperplasia with atypia. In the present study, we aimed to explore whether ghrelin expression had a role in distinguishing between benign, pre-malignant and malignant lesions of endometrium. Methods. Tissue ghrelin expressions of a total of 60 cases, who were diagnosed in the Pathology Department Laboratory of Fıırat University Medical School, and of whom 10 were in the proliferation phase, 10 had simple hyperplasia without atypia, 10 had simple hyperplasia with atypia, 10 had complex hyperplasia without atypia, 10 had complex hyperplasia with atypia and 10 had endometrioid carcinoma cases, were examined using immunohistochemical method. Additionally, tissue samples were homogenised to analyse tissue ghrelin levels in the supernatants according to RIA method. Samples from the parotid glands were used as positive control for ghrelin. Cells that exhibited cytoplasmic staining with ghrelin antibody were evaluated as positive. Results. Immunohistochemical examination showed that ghrelin expression increased markedly in the proliferation phase, relative to hyperplasias and carcinoma. These results were parallel to ghrelin levels in tissue supernatants. Immunohistochemical and RIA analysis results indicate that ghrelin expression either markedly decreases or is entirely depleted in endometrial carcinomas. Conclusions. Therefore, we think that ghrelin expression can be useful in differentiating not only endometrium carcinomas from benign lesions but also complex hyperplasias with atypia, which pose diagnostic difficulties. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
58. WT1 and DAX-1 regulate SF-1-mediated human P450arom gene expression in gonadal cells
- Author
-
Gurates, Bilgin, Amsterdam, Abraham, Tamura, Mitsutoshi, Yang, Sijun, Zhou, Jianfeng, Fang, Zongjuan, Amin, Sanober, Sebastian, Siby, and Bulun, Serdar E.
- Subjects
- *
TUMOR suppressor genes , *NEPHROBLASTOMA , *GONADS - Abstract
Binding activity of steroidogenic factor-1 (SF-1) to promoters of the majority of steroidogenic genes in response to gonadotropins is a critical mechanism that regulates steroidogenesis in gonads. Thus, the modulation of SF-1 action may be essential for the differential regulation of formation of sex steroids in the ovary. Aromatase P450 (P450arom) is the rate-limiting enzyme for estrogen formation. In this study, we characterize another nuclear receptor half site in the gonadal aromatase promoter which we show to be important for aromatase regulation. We also show herein that the stimulation of P450arom promoter activity by SF-1 in ovarian granulosa, testicular Sertoli and JEG-3 choriocarcinoma cells is inhibited by two transcription factors, Wilms’ tumor suppressor gene (WT1) and dosage sensitive sex reversal adrenal hypoplasia congenita critical region on the X chromosome gene 1 (DAX-1). Given the characterized roles of these transcription factors in gonadal development and function, modulation of SF-1 action by WT1 and DAX-1 may represent an important key mechanism in steroidogenesis. [Copyright &y& Elsevier]
- Published
- 2003
- Full Text
- View/download PDF
59. Vulvar Basal Cell Carcinoma with Cliloral Involvement.
- Author
-
Celik, Husnu, Gurates, Bilgin, Yavuz, Adem, Dogan, Zeynep, Cobanoglu, Bengu, and Saral, Yunus
- Subjects
- *
LETTERS to the editor , *BASAL cell carcinoma - Abstract
A letter to the editor is presented which discusses a medical case study of a 72-year-old woman who has a basal cell carcinoma and its treatment.
- Published
- 2009
- Full Text
- View/download PDF
60. Prostaglandin E2 Via Steroidogenic Factor-1 Coordinately Regulates Transcription of Steroidogenic Genes Necessary for Estrogen Synthesis in Endometriosis
- Author
-
Hideki Tokunaga, Gonca Imir, Erkut Attar, David Redwine, Dale B. Hales, Rukset Attar, Bilgin Gurates, Nobuo Yaegashi, M. Bertan Yilmaz, Serdar E. Bulun, Michael Putman, [Attar, Erkut -- Tokunaga, Hideki -- Imir, Gonca -- Yilmaz, M. Bertan -- Bulun, Serdar E.] Northwestern Univ, Feinberg Sch Med, Dept Obstet & Gynecol, Div Reprod Biol Res, Chicago, IL 60611 USA -- [Attar, Erkut] Istanbul Univ, Capa Sch Med, Dept Obstet & Gynecol, Istanbul, Turkey -- [Tokunaga, Hideki -- Yaegashi, Nobuo] Tohoku Univ, Dept Obstet & Gynecol, Sendai, Miyagi 9808575, Japan -- [Redwine, David] Cumhuriyet Univ, Sch Med, Dept Obstet & Gynecol, Sivas, Turkey -- [Redwine, David] St Charles Hosp, Dept Obstet & Gynecol, Bend, OR 97701 USA -- [Putman, Michael] Baylor Univ Hosp, Dept Obstet & Gynecol, Dallas, TX 75246 USA -- [Gurates, Bilgin] Firat Univ, Dept Obstet & Gynecol, Sch Med, TR-23169 Elazig, Turkey -- [Attar, Rukset] Yeditepe Univ Hosp, Dept Obstet & Gynecol, Istanbul, Turkey -- [Hales, Dale B.] Univ Illinois, Dept Mol Physiol & Biophys, Chicago, IL 60612 USA, and yilmaz, bertan -- 0000-0001-5558-3299
- Subjects
Steroidogenic factor 1 ,Transcription, Genetic ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Steroidogenic Factor 1 ,Endometrium ,Biochemistry ,chemistry.chemical_compound ,Endocrinology ,Obstetrics and gynaecology ,Cytochrome P-450 Enzyme System ,Ovarian Diseases ,Aromatase ,Promoter Regions, Genetic ,Cells, Cultured ,Progesterone ,Regulation of gene expression ,Estradiol ,biology ,Steroidogenic acute regulatory protein ,General Medicine ,Middle Aged ,University hospital ,medicine.anatomical_structure ,Female ,Original Article ,Adult ,endocrine system ,medicine.medical_specialty ,medicine.drug_class ,Endometriosis ,Estrone ,Dinoprostone ,Gene Expression Regulation, Enzymologic ,Young Adult ,Translational Highlights from Jcem ,Internal medicine ,medicine ,Humans ,Molecular Biology ,Estrogen synthesis ,business.industry ,urogenital system ,Biochemistry (medical) ,Promoter ,Phosphoproteins ,chemistry ,Estrogen ,Family medicine ,Molecular physiology ,biology.protein ,business - Abstract
WOS: 000263072700045, PubMed ID: 19001523, Context: Products of at least five specific steroidogenic genes, including steroidogenic acute regulatory protein (StAR), which facilitates the entry of cytosolic cholesterol into the mitochondrion, side chain cleavage P450 enzyme, 3 beta-hydroxysteroid-dehydrogenase-2, 17-hydroxylase/17-20-lyase, and aromatase, which catalyzes the final step, are necessary for the conversion of cholesterol to estrogen. Expression and biological activity of StAR and aromatase were previously demonstrated in endometriosis but not in normal endometrium. Prostaglandin E-2 (PGE(2)) induces aromatase expression via the transcriptional factor steroidogenic factor-1 (SF1) in endometriosis, which is opposed by chicken-ovalbumin upstream- transcription factor (COUP-TF) and Wilms' tumor-1 (WT1) in endometrium. Objective: The aim of the study was to demonstrate a complete steroidogenic pathway leading to estrogen biosynthesis in endometriotic cells and the transcriptional mechanisms that regulate basal and PGE(2)-stimulated estrogen production in endometriotic cells and endometrium. Results: Compared with normal endometrial tissues, mRNA levels of StAR, side chain cleavage P450, 3 beta-hydroxysteroid- dehydrogenase-2, 17-hydroxylase/17-20-lyase, aromatase, and SF1 were significantly higher in endometriotic tissues. PGE(2) induced the expression of all steroidogenic genes; production of progesterone, estrone, and estradiol; and StAR promoter activity in endometriotic cells. Overexpression of SF1 induced, whereas COUP-TFII or WT1 suppressed, StAR promoter activity. PGE(2) induced coordinate binding of SF1 to StAR and aromatase promoters but decreased COUP-TFII binding in endometriotic cells. COUP-TFII or WT1 binding to both promoters was significantly higher in endometrial compared with endometriotic cells. Conclusion: Endometriotic cells contain the full complement of steroidogenic genes for de novo synthesis of estradiol from cholesterol, which is stimulated by PGE(2) via enhanced binding of SF1 to promoters of StAR and aromatase genes in a synchronous fashion. (J Clin Endocrinol Metab 94: 623-631, 2009), National Institutes of Health [HD38691, HD40093]; Friends of Prentice, This work was supported by grants from the National Institutes of Health (HD38691 and HD40093) and Friends of Prentice.
- Published
- 2009
61. A case of vogt-koyanagi-harada disease associated with polycystic ovary syndrome.
- Author
-
Kaya MK, Turgut B, Demir T, Celiker U, and Gurates B
- Abstract
Unlabelled: We report a female patient diagnosed as Vogt-Koyanagi-Harada (VKH) and polycystic ovary syndrome (PCOS). She has diagnosed as VKH with diminished vision, bilateral serous retinal detachment, the signs of fundus fluorescein angiography and the findings of optical coherence tomography. The patient was referred to the gynecology clinic for her complaints as weight gain, hirsutismus and amenorrhea. She has also been diagnosed with PCOS. With oral steroid treatment, visual acuity has improved and the detachments have resolved within a month. VKH disease may be associated with polycystic ovary syndrome. The two conditions may have a common autoimmune pathogenesis., Keywords: Autoimmune pathogenesis; Polycystic ovary syndrome; Vogt-Koyanagi-Harada.
- Published
- 2011
- Full Text
- View/download PDF
62. Combating endometriosis by blocking proteasome and nuclear factor-kappaB pathways.
- Author
-
Celik O, Hascalik S, Elter K, Tagluk ME, Gurates B, and Aydin NE
- Subjects
- Animals, Antigens, CD34 biosynthesis, Azo Compounds pharmacology, Boronic Acids therapeutic use, Bortezomib, Case-Control Studies, Eosine Yellowish-(YS) pharmacology, Female, Ki-67 Antigen biosynthesis, Methyl Green pharmacology, Platelet Endothelial Cell Adhesion Molecule-1 biosynthesis, Proliferating Cell Nuclear Antigen biosynthesis, Pyrazines therapeutic use, Pyrrolidines therapeutic use, Rats, Thiocarbamates therapeutic use, Endometriosis drug therapy, NF-kappa B antagonists & inhibitors, Protease Inhibitors therapeutic use, Proteasome Inhibitors
- Abstract
Background: The objective of this study is to investigate the effect of pyrrolidine dithiocarbamate [PDTC; a nuclear factor-kappaB (NF-kappaB) inhibitor] and bortezomib (Velcade; a proteasome inhibitor) on the development of experimental endometriotic implants in rats., Methods: Endometriosis was surgically induced in 30 rats using the method of Vernon and Wilson. Three weeks later the viability and volume of the implants were recorded and classified. Afterwards, rats were put into three groups with equal numbers. The groups were labelled as the control, the PDTC and the bortezomib groups. Seven days after treatment, a third laparotomy was done and the volume of implants was measured again. The animals were then sacrificed, and the implants were stained with Ki67, proliferating cell nuclear antigen (PCNA), CD34, CD31 and Masson's trichrome histochemical staining., Results: In 80% of the implanted rats, vesicles at the suture region were observed, and the rats graded according to average vesicle diameter (D) as: Grade 1 (no vesicle, 20% of rats), Grade 2 (D < 2 mm, 33.3% of rats), Grade 3 (2 mm
4.5 mm, 26.7% of rats) and Grade 4 (D > 4.5 mm, 20% of rats). After treatment with PDTC or bortezomib, these percentages were decreased for Grades 3 and 4, and increased in Grade 1. The post-treatment implant volumes were decreased in the PDTC and bortezomib groups (P < 0.002 and P < 0.001), and slightly increased in the control group (P = 0.279). In the PDTC and bortezomib groups, CD34, CD31, PCNA and Ki67 expression levels were similar but were significantly reduced compared with the control group., Conclusions: PDTC and bortezomib may represent a novel therapeutic strategy for treatment of endometriosis. - Published
- 2008
- Full Text
- View/download PDF
63. The effect of circadian rhythm changes on fetal and placental development (experimental study).
- Author
-
Gozeri E, Celik H, Ozercan I, Gurates B, Polat SA, and Hanay F
- Subjects
- Animals, Female, Fetal Weight physiology, Models, Animal, Organ Size, Photoperiod, Placenta pathology, Placenta physiology, Pregnancy, Random Allocation, Rats, Rats, Wistar, Circadian Rhythm physiology, Fetal Development physiology, Placentation physiology
- Abstract
Objective: To investigate the effect of circadian rhythm changes on fetal-placental development in pregnant rats., Material and Method: Pregnant rats were randomly allocated to 4 groups with 10 rats in each group. Group 1: 12 hours light 12 hours dark period (n:10, Control Group); Group 2: 6 hours light 6 hours dark period (n:10); Group 3: constant light group (n:10); Group 4: constant dark group (n:10). Rats were made to deliver by cesarean birth at the end of their pregnancy and their placenta, number and weight of the offspring, and placental histopathological changes were examined., Finding: Fetal number in the constant dark group was found significant in comparison to constant light group (p<0.01). Fetal weight in all groups with altered circadian rhythm was significantly lower than that in the control group (p<0.01). The decrease in fetal weight was more evident in the constant light group than in the constant dark group (p<0.01). Comparison of the histopathological findings of the placenta showed that placental edema, fibrin accumulation and leukocyte infiltration were significantly higher in constant light and constant dark groups than in other groups (p<0.01)., Conclusion: Circadian rhythm change in pregnancy adversely affects the fetal-placental development. More detailed studies are needed to clarify this mechanism.
- Published
- 2008
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.