68 results on '"Heltai, Silvia"'
Search Results
52. Constitutive expression of the heat shock protein 72 kDa in human melanoma cells
- Author
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Protti, Maria Pia, primary, Heltai, Silvia, additional, Bellone, Matteo, additional, Ferrarini, Marina, additional, Manfredi, Angelo A., additional, and Rugarli, Claudio, additional
- Published
- 1994
- Full Text
- View/download PDF
53. Unusual expression and localization of heat-shock proteins in human tumor cells
- Author
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Ferrarini, Marina, primary, Heltai, Silvia, additional, Zocchi, M. Raffaella, additional, and Rugarli, Claudio, additional
- Published
- 1992
- Full Text
- View/download PDF
54. Nef-specific CD45RA+ CD8+ T cells secretingMIP-1β but not IFN-γ are associated withnonprogressive HIV-1 infection.
- Author
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Dembek, Claudia J, Kutscher, Sarah, Heltai, Silvia, Allgayer, Simone, Biswas, Priscilla, Ghezzi, Silvia, Vicenzi, Elisa, Hoffmann, Dieter, Reitmeir, Peter, Tambussi, Giuseppe, Bogner, Johannes R., Lusso, Paolo, Stellbrink, Hans-J, Santagostino, Elena, Vollbrecht, Thomas, Goebel, Frank D., Protzer, Ulrike, Draenert, Rika, Tinelli, Marco, and Poli, Guido
- Subjects
HIV-positive persons ,HIV infections ,ANTIRETROVIRAL agents ,T cells ,MEDICAL research ,BIOTECHNOLOGY - Abstract
Background: Long-term survival of HIV-1 infected individuals is usually achieved by continuous administration of combination antiretroviral therapy (ART). An exception to this scenario is represented by HIV-1 infected nonprogressors (NP) which maintain relatively high circulating CD4+ T cells without clinical symptoms for several years in the absence of ART. Several lines of evidence indicate an important role of the T-cell response in the modulation of HIV-1 infection during the acute and chronic phase of the disease. Results: We analyzed the functional and the differentiation phenotype of Nef- and Tat-specific CD8+ T cells in a cohort of HIV-1 infected NP in comparison to progressors, ART-treated seropositive individuals and individuals undergoing a single cycle of ART interruption. We observed that a distinctive feature of NP is the presence of Nef-specific CD45RA+ CD8+ T cells secreting MIP-1beta but not IFN-gamma. This population was present in 7 out of 11 NP. CD45RA+ IFNgamma
neg MIP-1beta+ CD8+ T cells were not detected in HIV-1 infected individuals under ART or withdrawing from ART and experiencing a rebounding viral replication. In addition, we detected Nef-specific CD45RA+ IFN-gammaneg MIP-1beta+ CD8+ T cells in only 1 out of 10 HIV-1 infected individuals with untreated progressive disease. Conclusion: The novel antigen-specific CD45RA+ IFN-gammaneg MIP-1beta+ CD8+ T cell population represents a new candidate marker of long-term natural control of HIV-1 disease progression and a relevant functional T-cell subset in the evaluation of the immune responses induced by candidate HIV-1 vaccines. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
55. Autoimmune Thyroiditis following Interleukin-2 and Lak Cell Therapy for Metastatic Renal Cell Carcinoma: Correlation with Tumor Regression
- Author
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Besana, Carlo, primary, Sabbadini, Maria Grazia, additional, Corti, Consuelo, additional, Di Lucca, Giuseppe, additional, Foppoli, Marco, additional, Marcatti, Magda, additional, Heltai, Silvia, additional, and Rugarli, Claudio, additional
- Published
- 1991
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- View/download PDF
56. Engagement of CD30 shapes the secretion of cytokines by human ??? T cells.
- Author
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Biswas, Priscilla, Rovere, Patrizia, Filippi, Camilla?De, Heltai, Silvia, Smith, Craig, Dagna, Lorenzo, Poli, Guido, Manfredi, Angelo?A., and Ferrarini, Marina
- Published
- 2000
- Full Text
- View/download PDF
57. Apoptotic cell clearance in systemic lupus erythematosus: II. Role of β2-glycoprotein I.
- Author
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Manfredi, Angelo A., Rovere, Patrizia, Heltai, Silvia, Galati, Giacomo, Nebbia, Gaia, Tincani, Angela, Balestrieri, Genesio, and Sabbadini, Maria Grazia
- Published
- 1998
- Full Text
- View/download PDF
58. Evidence for the Involvement of Phosphatidylinositol 3Kinase in fMLPStimulated Neutrophil Adhesion to ICAM1Transfected Cells
- Author
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Pellegatta, Fabio, Radaelli, Antonella, Heltai, Silvia, Yan, Lu, Chierchia, Sergio L., and Folli, Franco
- Abstract
Phosphatidylinositol 3kinase PI3K controls important intracellular steps involved in inflammation, immunity, and cell growth. PI3K also modulates leukocyte integrin adhesiveness. In this study we evaluated the role of PI3K on neutrophil adhesion to intercellular adhesion molecule1 ICAM1transfected cells. Nformylmethionylleucylphenylalanine fMLPstimulated neutrophil adhesion was inhibited by wortmannin and LY294002, two unrelated PI3K inhibitors, whereas phorbol myristate acetate PMAinduced neutrophil adhesion was not inhibited by them. After fMLP stimulation, a rapid activation of AKT and ERK was observed. However, only activation of AKT was reversed by the PI3K inhibitors. Neutrophil expression of the 2integrins Mac1, lymphocyte functionassociated antigen1LFA1, and gp150.95 was not affected by wortmannin, nor was expression of the activation epitope recognized by MAB24. We conclude that a PI3K is involved in fMLPactivated neutrophil adhesion to ICAM1transfected cells, b the mechanism involved is not mediated by the modulation of 2integrin expression or activation, and c another mechanism seems to involve the adhesion to ICAM1 when a cellular system of adhesion is used.
- Published
- 2001
59. In vivoadministration of GM‐CSF promotes the clearance of apoptotic cells: effects on monocytes and polymorphonuclear leukocytes
- Author
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Galati, Giacomo, Rovere, Patrizia, Citterio, Giovanni, Bondanza, Attilio, Scaglietti, Ugo, Bucci, Eraldo, Heltai, Silvia, Fascio, Umberto, Rugarli, Claudio, and Manfredi, Angelo A.
- Abstract
The clearance of apoptotic cells is crucial to avoid chronic inflammation and autoimmunity. Little is known about the factors that regulate it in vivo.We show that granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) administration to carcinoma patients confers to their leukocytes a significantly higher ability to phagocytose apoptotic cells than before (P< 0.005). GM‐CSF increased the concentration of monocytes and polymorphonuclear leukocytes in the peripheral blood and activated circulating polymorphonuclear leukocytes. Both effects abated early after treatment, whereas phagocytosis of apoptotic cells was still significantly higher after 18 days compared with basal values (P< 0.005 and P< 0.025 for monocytes and polymorphonuclear leukocytes, respectively). On in vitrophagocytosis of apoptotic cells monocytes, but not polymorphonuclear leukocytes, up‐regulated MHC class II membrane expression. These findings are consistent with the possibility that GM‐CSF endows both scavenger and antigen‐presenting leukocytes with the ability to internalize apoptotic tumor cells. J. Leukoc. Biol.67: 174–182; 2000.
- Published
- 2000
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60. Human Melanoma Cells Transfected with the B7-2 Co-Stimulatory Molecule Induce Tumor-Specific CD8Cytotoxic T Lymphocytes In Vitro
- Author
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Imro, Maria Adele, Dellabona, Paolo, Manici, Simona, Heltai, Silvia, Consogno, Giuseppe, Bellone, Matteo, Rugarli, Claudio, and Protti, Maria Pia
- Abstract
ABSTRACTNeoplastic cells express tumor-associated antigens, but tumor rejection seldom occurs in vivo. The absence of an effective immune response may be explained by the inability of tumor cells to deliver co-stimulatory signals. Indeed, transfection of either B7-1 or B7-2 co-stimulatory molecules into mouse tumor cells enhances antitumor immune responses. In this study, we stably transfected human melanoma cells with the cDNA encoding the B7-2 molecule to evaluate in vitro:(i) the induction of anti-melanoma cytotoxic T lymphocytes (CTL) by stimulation of CD8T cells, purified from healthy donors and a melanoma patient, with B7-2 transfected allogeneic HLA-matched melanoma cells; (ii) the tumor specificity and the HLA restriction of the induced CTL; and (iii) the feasibility to propagate long-term antimelanoma CTL lines. We found that B7-2 transfected, but not untransfected or mock-transfected, melanoma cells activated MHC-class I-restricted, melanoma-specific CD8CTL from healthy donors. More importantly, CD8tumor-associated lymphocytes, purified from a tumor-invaded lymph node of a melanoma patient and stimulated with B7-2-transfected melanoma cells, acquired a strong reactivity toward the autologous tumor. CTL lines with specific cytolytic activity could be propagated in long-term culture. These results indicate that: (i) the expression of the B7-2 molecule into human melanoma cells makes them immunogenic and able to act as antigen-presenting cells and (ii) purified CD8cells, stimulated with B7-2allogeneic HLA-matched melanoma cells, preferentially recognize melanoma-specific rather than allogeneic antigens. This study may have clinical implications for passive and/or active immunotherapy in melanoma patients.
- Published
- 1998
- Full Text
- View/download PDF
61. Mycobacterium tuberculosis exploits the CD95/CD95 ligand system of γ δ T cells to cause apoptosis
- Author
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Manfredi, Angelo A., Heltai, Silvia, Rovere, Patrizia, Sciorati, Clara, Paolucci, Clara, Galati, Giacomo, Rugarli, Claudio, Vaiani, Roberto, Clementi, Emilio, and Ferrarini, Marina
- Abstract
Vγ9/Vδ2+ T cells specifically recognize Mycobacterium tuberculosis in vitro and are precociously recruited in early mycobacterial lesions. Even if γ δ T cells are only fortuitously detected in granulomas or bronchoalveolar lavages of patients with active pulmonary tuberculosis, a role in shaping the mature α β T cell response against M. tuberculosis is substantiated. Here we provide a molecular explanation for this paradox: the engagement of the γ δ TCR by mycobacterial antigens induced the expression of CD95 ligand (CD95L) by chronically activated CD95+ /CD95L− γ δ T lymphocytes. The receptor was functional, as CD95/CD95L interaction triggered the bystander death of CD95+ cells by apoptosis. Cell death was abolished by CD95-blocking antibodies. The transient accumulation at the site of infection of CD95L+ γ δ lymphocytes, capable of interacting with CD95+ leukocytes attracted by the response towards the pathogen, may determine the characteristics of the ensuing granulomatous disease.
- Published
- 1998
- Full Text
- View/download PDF
62. Dual-parameter flow cytometric analysis of an early lymphocyte activation antigen (CK226) and DNA content
- Author
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Zocchi, M. Raffaella, primary, Heltai, Silvia, additional, and Poggi, Alessandro, additional
- Published
- 1989
- Full Text
- View/download PDF
63. Role of interleukin-2 in regulating lymphocyte activation and recirculation
- Author
-
Fortis, Claudio, Ferrero, Elisabetta, Heltai, Silvia, Besana, Carlo, Corti, Consuelo, Di Lucca, Giuseppe, Foppoli, Marco, Consogno, Giuseppe, and Rugarli, Claudio
- Published
- 1993
- Full Text
- View/download PDF
64. Modeling multiple myeloma-bone marrow interactions and response to drugs in a 3d surrogate microenvironment
- Author
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Stefania Girlanda, Marina Ferrarini, Lorenza Pecciarini, Elisabetta Ferrero, Federico Caligaris-Cappio, Magda Marcatti, Giovanni Tonon, Maurilio Ponzoni, Antonello Villa, Silvia Heltai, Barbara Vergani, Fabio Ciceri, Daniela Belloni, Belloni, D, Heltai, S, Ponzoni, M, Villa, A, Vergani, B, Pecciarini, L, Marcatti, M, Girlanda, S, Tonon, G, Ciceri, F, Caligaris-Cappio, F, Ferrarini, M, Ferrero, E, Belloni, Daniela, Heltai, Silvia, Ponzoni, Maurilio, Villa, Antonello, Vergani, Barbara, Pecciarini, Lorenza, Marcatti, Magda, Girlanda, Stefania, Tonon, Giovanni, Ciceri, Fabio, Caligaris-Cappio, Federico, Ferrarini, Marina, and Ferrero, Elisabetta
- Subjects
0301 basic medicine ,3D model ,medicine.medical_specialty ,Stromal cell ,Cell Survival ,Cell Culture Techniques ,Drug Resistance ,Cell Communication ,Models, Biological ,Article ,Plasma Cell Disorders ,Bortezomib ,03 medical and health sciences ,Bioreactors ,0302 clinical medicine ,Bone Marrow ,immune system diseases ,hemic and lymphatic diseases ,Internal medicine ,Cell Adhesion ,Tumor Microenvironment ,medicine ,Humans ,Multiple myeloma ,Tumor microenvironment ,Hematology ,Chemistry ,Endothelial Cells ,medicine.disease ,Coculture Techniques ,Bone Marrow Microenvironment ,030104 developmental biology ,medicine.anatomical_structure ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Gelatin ,Bone marrow ,Stromal Cells ,Clone (B-cell biology) ,Multiple Myeloma ,medicine.drug - Abstract
Multiple myeloma develops primarily inside the bone marrow microenvironment, that confers pro-survival signals and drug resistance. 3D cultures that reproduce multiple myeloma-bone marrow interactions are needed to fully investigate multiple myeloma pathogenesis and response to drugs. To this purpose, we exploited the 3D Rotary Cell Culture System bioreactor technology for myeloma-bone marrow co-cultures in gelatin scaffolds. The model was validated with myeloma cell lines that, as assessed by histochemical and electron-microscopic analyses, engaged contacts with stromal cells and endothelial cells. Consistently, pro-survival signaling and also cell adhesion-mediated drug resistance were significantly higher in 3D than in 2D parallel co-cultures. The contribution of the VLA-4/VCAM1 pathway to resistance to bortezomib was modeled by the use of VCAM1 transfectants. Soluble factor-mediated drug resistance could be also demonstrated in both 2D and 3D co-cultures. The system was then successfully applied to co-cultures of primary myeloma cells-primary myeloma bone marrow stromal cells from patients and endothelial cells, allowing the development of functional myeloma-stroma interactions and MM cell long-term survival. Significantly, genomic analysis performed in a high-risk myeloma patient demonstrated that culture in bioreactor paralleled the expansion of the clone that ultimately dominated in vivo. Finally, the impact of bortezomib on myeloma cells and on specialized functions of the microenvironment could be evaluated. Our findings indicate that 3D dynamic culture of reconstructed human multiple myeloma microenvironments in bioreactor may represent a useful platform for drug testing and for studying tumor-stroma molecular interactions.
- Published
- 2018
65. Basophil Recruitment into Tumor-Draining Lymph Nodes Correlates with Th2 Inflammation and Reduced Survival in Pancreatic Cancer Patients
- Author
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Hana Algül, Gilda Magliacane, Emanuela Brunetto, Helios Recalde, Lucia De Monte, Francesca Aleotti, Silvia Heltai, Anna Mondino, Massimo Falconi, Anna Maria Paganoni, Michele Reni, Maria Pia Protti, S Wörmann, Claudio Doglioni, Gianpaolo Balzano, De Monte, Lucia, Wormann, Sonja, Brunetto, Emanuela, Heltai, Silvia, Magliacane, Gilda, Reni, Michele, Paganoni Anna, Maria, Recalde, Helio, Mondino, Anna, Falconi, Massimo, Aleotti, Francesca, Balzano, Gianpaolo, Ul Hana, Alg, Doglioni, Claudio, and Protti Maria, Pia
- Subjects
0301 basic medicine ,Cancer Research ,chemical and pharmacologic phenomena ,Inflammation ,Basophil ,03 medical and health sciences ,Th2 Cells ,0302 clinical medicine ,Immune system ,Pancreatic cancer ,parasitic diseases ,medicine ,Humans ,Interleukin 4 ,Aged ,Neoplasm Staging ,Retrospective Studies ,Tumor microenvironment ,business.industry ,Cancer ,hemic and immune systems ,Middle Aged ,medicine.disease ,Basophils ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Tumor progression ,030220 oncology & carcinogenesis ,Immunology ,Lymph Nodes ,medicine.symptom ,business ,Carcinoma, Pancreatic Ductal - Abstract
In pancreatic ductal adenocarcinomas (PDAC), lymphoid infiltrates, comprised mainly of Th2 cells, predict a poor survival outcome in patients. IL4 signaling has been suggested to stabilize the Th2 phenotype in this setting, but the cellular source of IL4 in PDAC is unclear. Here, we show that basophils expressing IL4 are enriched in tumor-draining lymph nodes (TDLN) of PDAC patients. Basophils present in TDLNs correlated significantly with the Th2/Th1 cell ratio in tumors, where they served as an independent prognostic biomarker of patient survival after surgery. Investigations in mouse models of pancreatic cancer confirmed a functional role for basophils during tumor progression. The recruitment of basophils into TDLN relied partly upon the release of chemokine CCL7/MCP3 by "alternatively activated" monocytes, whereas basophil activation was induced by T-cell–derived IL3. Our results show how basophils recruited and activated in TDLNs under the influence of the tumor microenvironment regulate tumor-promoting Th2 inflammation in PDAC, helping in illuminating a key element of the immune milieu of pancreatic cancer. Cancer Res; 76(7); 1792–803. ©2016 AACR.
- Published
- 2016
66. The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting providing a sensitive alternative to the ELISPOT
- Author
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Claudia J. Dembek, Volker Erfle, Giuseppe Tambussi, Priscilla Biswas, Paolo Lusso, Simone Allgayer, Heike Pohla, Hans Jürgen Stellbrink, Frank D. Goebel, Marco Tinelli, Johannes R. Bogner, Antonio Cosma, Guido Poli, Sarah Kutscher, Birgit Stadlbauer, Silvia Heltai, Mauro S. Malnati, Silvia Nozza, Kutscher, Sarah, Dembek Claudia, J, Allgayer, Simone, Heltai, Silvia, Stadlbauer, Birgit, Biswas, Priscilla, Nozza, Silvia, Tambussi, Giuseppe, Bogner Johannes, R, Stellbrink Hans, J, Goebel Frank, D, Lusso, Paolo, Tinelli, Marco, Poli, Guido, Erfle, Volker, Pohla, Heike, Malnati, Mauro, and Cosma, Antonio
- Subjects
lcsh:Immunologic diseases. Allergy ,biology ,ELISPOT ,Methodology ,Acquired immune system ,Immune system ,Immunity ,Virology ,Immunology ,biology.protein ,Molecular Medicine ,Cytotoxic T cell ,Pharmacology (medical) ,Antibody ,lcsh:RC581-607 ,CD8 ,Intracellular - Abstract
Background T-cell mediated immunity likely plays an important role in controlling HIV-1 infection and progression to AIDS. Several candidate vaccines against HIV-1 aim at stimulating cellular immune responses, either alone or together with the induction of neutralizing antibodies, and assays able to measure CD8 and CD4 T-cell responses need to be implemented. At present, the IFN-γ-based ELISPOT assay is considered the gold standard and it is broadly preferred as primary assay for detection of antigen-specific T-cell responses in vaccine trials. However, in spite of its high sensitivity, the measurement of the sole IFN-γ production provides limited information on the quality of the immune response. On the other hand, the introduction of polychromatic flow-cytometry-based assays such as the intracellular cytokine staining (ICS) strongly improved the capacity to detect several markers on a single cell level. Results The cumulative analysis of 275 samples from 31 different HIV-1 infected individuals using an ICS staining procedure optimized by our laboratories revealed that, following antigenic stimulation, IFN-γ producing T-cells were also producing MIP-1β whereas T-cells characterized by the sole production of IFN-γ were rare. Since the analysis of the combination of two functions decreases the background and the measurement of the IFN-γ+ MIP-1β+ T-cells was equivalent to the measurement of the total IFN-γ+ T-cells, we adopted the IFN-γ+ MIP-1β+ data analysis system to evaluate IFN-γ-based, antigen-specific T-cell responses. Comparison of our ICS assay with ELISPOT assays performed in two different experienced laboratories demonstrated that the IFN-γ+ MIP-1β+ data analysis system increased the sensitivity of the ICS up to levels comparable to the sensitivity of the ELISPOT assay. Conclusion The IFN-γ+ MIP-1β+ data evaluation system provides a clear advantage for the detection of low magnitude HIV-1-specific responses. These results are important to guide the choice for suitable highly sensitive immune assays and to build reagent panels able to accurately characterize the phenotype and function of responding T-cells. More importantly, the ICS assay can be used as primary assay to evaluate HIV-1-specific responses without losing sensitivity in comparison to the ELISPOT assay.
- Published
- 2008
67. Nef Alleles from Human Immunodeficiency Virus Type 1-Infected Long-Term-Nonprogressor Hemophiliacs with or without Late Disease Progression Are Defective in Enhancing Virus Replication and CD4 Down-Regulation.
- Author
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Crotti, Andrea, Neri, Francesca, Corti, Davide, Ghezzi, Silvia, Heltai, Silvia, Baur, Andreas, Poli, Guido, Santagostino, Elena, and Vicenzi, Elisa
- Subjects
- *
HIV , *VIRAL replication , *CELL membranes , *IMMUNOGENETICS , *GENETIC mutation - Abstract
Infection with human immunodeficiency virus (HIV)-encoding defective nef variants may contribute to a relatively benign course of disease in a minority of long-term nonprogressors (LTNP). We have examined the functions of nef alleles from six individuals belonging to the same cohort of hemophiliacs infected with HIV-1 prior to 1985 and classified as LTNP in 1995. Three out of six individuals have progressed to HIV disease (late progressors [LP]), whereas the three remainders have maintained their LTNP status at least up to 2003. The nef alleles were obtained from both plasma virus and peripheral blood mononuclear cells of all six individuals in 1995 and 1998. The proportion of sequences containing mutations not yielding Nef expression significantly diminished in 1998 versus that in 1995. Several previously defined functional regions of intact nef alleles were highly conserved. However, the major variant obtained in 1998 from plasma RNA of five out of six individuals significantly reduced HIV infectivity/replication and impaired Nef-mediated CD4 but not major histocompatibility complex class I antigen down-modulation from the cell surface. Thus, functional alterations of the nef gene are present in both LP and LTNP, suggesting that Nef defectiveness in vitro is not necessarily associated with the long-term maintenance of LTNP status. Of interest is the fact that isolates from three out of three LP showed a dual CCR5/CXCR4 coreceptor use (R5X4), in contrast to those from LTNP, which were exclusively R5. Thus, in vivo evolution of gp120 Env to CXCR4 use appears to be associated with HIV disease progression in individuals infected with nef-defective viruses. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
68. Nef-specific CD45RA+ CD8+ T cells secreting MIP-1beta but not IFN-gamma are associated with nonprogressive HIV-1 infection.
- Author
-
Dembek CJ, Kutscher S, Heltai S, Allgayer S, Biswas P, Ghezzi S, Vicenzi E, Hoffmann D, Reitmeir P, Tambussi G, Bogner JR, Lusso P, Stellbrink HJ, Santagostino E, Vollbrecht T, Goebel FD, Protzer U, Draenert R, Tinelli M, Poli G, Erfle V, Malnati M, and Cosma A
- Abstract
Background: Long-term survival of HIV-1 infected individuals is usually achieved by continuous administration of combination antiretroviral therapy (ART). An exception to this scenario is represented by HIV-1 infected nonprogressors (NP) which maintain relatively high circulating CD4+ T cells without clinical symptoms for several years in the absence of ART. Several lines of evidence indicate an important role of the T-cell response in the modulation of HIV-1 infection during the acute and chronic phase of the disease., Results: We analyzed the functional and the differentiation phenotype of Nef- and Tat-specific CD8+ T cells in a cohort of HIV-1 infected NP in comparison to progressors, ART-treated seropositive individuals and individuals undergoing a single cycle of ART interruption. We observed that a distinctive feature of NP is the presence of Nef-specific CD45RA+ CD8+ T cells secreting MIP-1beta but not IFN-gamma. This population was present in 7 out of 11 NP. CD45RA+ IFN-gammaneg MIP-1beta+ CD8+ T cells were not detected in HIV-1 infected individuals under ART or withdrawing from ART and experiencing a rebounding viral replication. In addition, we detected Nef-specific CD45RA+ IFN-gammaneg MIP-1beta+ CD8+ T cells in only 1 out of 10 HIV-1 infected individuals with untreated progressive disease., Conclusion: The novel antigen-specific CD45RA+ IFN-gammaneg MIP-1beta+ CD8+ T cell population represents a new candidate marker of long-term natural control of HIV-1 disease progression and a relevant functional T-cell subset in the evaluation of the immune responses induced by candidate HIV-1 vaccines.
- Published
- 2010
- Full Text
- View/download PDF
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