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316 results on '"Hempel, Ute"'

51. Functional und structural insights into TIMP-3- glycosaminoglycan interactions - implications for the design of functional biomaterials improving chronic wound treatment

Author

Moeller Stephanie, Hintze Vera, Hofmann Tommy, Schnabelrauch Matthias, Rademann Jrg, Von Bergen Martin, Pisabarro Mayte, Scharnweber Dieter, Hempel Ute, Blaszkiewicz Joanna, Rother Sandra, Samsonov Sergey, and Kalkof Stefan

Subjects
Chronic wound, Glycosaminoglycan, Histology, business.industry, Biomedical Engineering, medicine, Bioengineering, medicine.symptom, Bioinformatics, business, Biotechnology
Published
2016

55. Hyaluronan/Collagen Hydrogels with Sulfated Hyaluronan for Improved Repair of Vascularized Tissue Tune the Binding of Proteins and Promote Endothelial Cell Growth

Author

Rother, Sandra, primary, Galiazzo, Vanessa D., additional, Kilian, David, additional, Fiebig, Karen M., additional, Becher, Jana, additional, Moeller, Stephanie, additional, Hempel, Ute, additional, Schnabelrauch, Matthias, additional, Waltenberger, Johannes, additional, Scharnweber, Dieter, additional, and Hintze, Vera, additional

Published
2017
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56. Sulfated Hyaluronan Derivatives Modulate TGF-β1:Receptor Complex Formation: Possible Consequences for TGF-β1 Signaling

Author

Koehler, Linda, primary, Samsonov, Sergey, additional, Rother, Sandra, additional, Vogel, Sarah, additional, Köhling, Sebastian, additional, Moeller, Stephanie, additional, Schnabelrauch, Matthias, additional, Rademann, Jörg, additional, Hempel, Ute, additional, Pisabarro, M. Teresa, additional, Scharnweber, Dieter, additional, and Hintze, Vera, additional

Published
2017
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58. Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts

Author

Hempel, Ute, Preissler, Carolin, Vogel, Sarah, Möller, Stephanie, Hintze, Vera, Becher, Jana, Schnabelrauch, Matthias, Rauner, Martina, Hofbauer, Lorenz C., and Dieter, Peter

Subjects
Adult, Male, Osteoblasts, Article Subject, Stem Cells, lcsh:R, lcsh:Medicine, Cell Differentiation, Mesenchymal Stem Cells, glycosaminoglycans, künstlich, extrazellulär, Matrix, GAG, Derivate, TU Dresden, Publikationsfonds, Alkaline Phosphatase, glycosaminoglycans,artificial extracellular matrices, Oversulfated, GAG, derivatives , Osteoblasts, Technical University Dresden, Publication funds, Extracellular Matrix, Calcification, Physiologic, Animals, Humans, Calcium, Cattle, Female, ddc:610, Rats, Wistar, Cell Shape, Cells, Cultured, Research Article, Glycosaminoglycans
Abstract

Sulfated glycosaminoglycans (GAG) are components of the bone marrow stem cell niche and to a minor extent of mature bone tissue with important functions in regulating stem cell lineage commitment and differentiation. We anticipated that artificial extracellular matrices (aECM) composed of collagen I and synthetically oversulfated GAG derivatives affect preferentially the differentiation of osteoblast-precursor cells and early osteoblasts. A set of gradually sulfated chondroitin sulfate and hyaluronan derivatives was used for the preparation of aECM. All these matrices were analysed with human bone marrow stromal cells to identify the most potent aECM and to determine the influence of the degree and position of sulfate groups and the kind of disaccharide units on the osteogenic differentiation. Oversulfated GAG derivatives with a sulfate group at the C-6 position of the N-acetylglycosamine revealed the most pronounced proosteogenic effect as determined by tissue nonspecific alkaline phosphatase activity and calcium deposition. A subset of the aECM was further analysed with different primary osteoblasts and cell lines reflecting different maturation stages to test whether the effect of sulfated GAG derivatives depends on the maturation status of the cells. It was shown that the proosteogenic effect of aECMwasmost prominent in early osteoblasts. [ABSTRACT FROM AUTHOR]

Published
2014

62. Metal release and cell biological compatibility of beta‐type Ti‐40Nb containing indium.

Author

Pilz, Stefan, Gebert, Annett, Voss, Andrea, Oswald, Steffen, Göttlicher, Markus, Hempel, Ute, Eckert, Jürgen, Rohnke, Marcus, Janek, Jürgen, and Calin, Mariana

Abstract

Abstract: Small indium (In) additions up to 5 wt % to the beta‐type Ti‐40Nb alloy effectively improve its mechanical biofunctionality. The impact on its biocompatibility is addressed in this work. Comparative electrochemical polarization studies and inductively coupled plasma optical emission spectrometry analyses were conducted in Tris‐buffered saline (on the basis of 150 mM NaCl) with pH 7.6 and 2.0 at 310 ± 1 K with Ti‐6Al‐4V as reference. The metal ion releases from beta‐type alloys were generally very low, for example, those of In3+ ions from (Ti‐40Nb)‐4In specimens were below 6 × 10−7 mmol/cm2. X‐ray photoelectron spectroscopy revealed the passivation mainly by Ti‐ and Nb‐oxides with traces of In‐oxides as the dominating surface process. In vitro studies demonstrate a better human bone marrow stromal cells (hBMSC) activity on the beta‐type alloys in comparison to CP‐Ti (grade 2), which is mainly due to their high Nb content. At 24 h after seeding on (Ti‐40Nb)‐4In the metabolic activity of hBMSC was 1.5‐fold higher and after 11 days, the tissue non‐specific alkaline phosphatase activity was 1.8‐fold higher relative to values for CP‐Ti. Surface treatments, like chemical etching or plasma oxidation, change the surface topography and the thickness and composition of the oxide layers, but they are not effective in further improving the cell response. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1686–1697, 2018. [ABSTRACT FROM AUTHOR]

Published
2018
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63. A supplement‐free osteoclast–osteoblast co‐culture for pre‐clinical application.

Author

Schulze, Sabine, Wehrum, Diana, Dieter, Peter, and Hempel, Ute

Subjects
MESENCHYMAL stem cells, OSTEOBLASTS, REGENERATION (Biology), CELL culture, IN vitro studies
Abstract

There is increasing demand for efficient and physiological in vitro cell culture systems suitable for testing new pharmaceutical drugs or for evaluating materials for tissue regeneration. In particular, co‐cultures of two or more tissue‐relevant cell types have the advantage to study the response of cells on diverse parameters in a more natural environment with respect to physiological complexity. We developed a direct bone cell co‐culture system using human peripheral blood monocytes (hPBMC) and human bone marrow stromal cells (hBMSC) as osteoclast/osteoblast precursor cells, respectively, strictly avoiding external supplements for the induction of differentiation. The sophisticated direct hPBMC/hBMSC co‐culture was characterized focusing on osteoclast function and was compared with two indirect approaches. Only in the direct co‐culture, hPBMC were triggered by hBMSC into osteoclastogenesis and became active resorbing osteoclasts. Bisphosphonates and sulfated glycosaminoglycans were used to examine the suitability of the co‐culture system for evaluating the influence of certain effectors on bone healing and bone regeneration and the contribution of each cell type thereby. The results show that the investigated substances had more pronounced effects on both osteoblasts and osteoclasts in the co‐culture system than in respective monocultures. [ABSTRACT FROM AUTHOR]

Published
2018
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71. Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts

Author

Hempel, Ute, primary, Preissler, Carolin, additional, Vogel, Sarah, additional, Möller, Stephanie, additional, Hintze, Vera, additional, Becher, Jana, additional, Schnabelrauch, Matthias, additional, Rauner, Martina, additional, Hofbauer, Lorenz C., additional, and Dieter, Peter, additional

Published
2014
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72. Proteomics and Metabolomics forIn SituMonitoring of Wound Healing

Author

Kalkhof, Stefan, primary, Förster, Yvonne, additional, Schmidt, Johannes, additional, Schulz, Matthias C., additional, Baumann, Sven, additional, Weißflog, Anne, additional, Gao, Wenling, additional, Hempel, Ute, additional, Eckelt, Uwe, additional, Rammelt, Stefan, additional, and von Bergen, Martin, additional

Published
2014
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77. Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool for In Vitro Osteogenesis and Bone Tissue Engineering Approaches.

Author

Hempel, Ute, Müller, Katrin, Preissler, Carolin, Noack, Carolin, Boxberger, Sabine, Dieter, Peter, Bornhäeuser, Martin, and Wobus, Manja

Subjects
*MESENCHYMAL stem cells, *BONE growth, *TISSUE engineering, *STEM cell donors, *STATISTICAL correlation, *RUNX proteins, *PARATHYROID hormone
Abstract

Adult human bone marrow stromal cells (hBMSC) are important for many scientific purposes because of their multipotency, availability, and relatively easy handling. They are frequently used to study osteogenesis in vitro. Most commonly, hBMSC are isolated from bone marrow aspirates collected in clinical routine and cultured under the “aspect plastic adherence” without any further selection. Owing to the random donor population, they show a broad heterogeneity. Here, the osteogenic differentiation potential of 531 hBMSC was analyzed. The data were supplied to correlation analysis involving donor age, gender, and body mass index. hBMSC preparations were characterized as follows: (a) how many passages the osteogenic characteristics are stable in and (b) the influence of supplements and culture duration on osteogenic parameters (tissue nonspecific alkaline phosphatase (TNAP), octamer binding transcription factor 4, core-binding factor alpha-1, parathyroid hormone receptor, bone gla protein, and peroxisome proliferator-activated protein γ). The results show that no strong prediction could be made from donor data to the osteogenic differentiation potential; only the ratio of induced TNAP to endogenous TNAP could be a reliable criterion. The results give evidence that hBMSC cultures are stable until passage 7 without substantial loss of differentiation potential and that established differentiation protocols lead to osteoblast-like cells but not to fully authentic osteoblasts. [ABSTRACT FROM AUTHOR]

Published
2016
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82. Regulation of bone mass and osteoclast function depend on the F-actin modulator SWAP-70

Author

Garbe, Annette I, primary, Roscher, Anne, additional, Schüler, Christiane, additional, Lutter, Anne-Helen, additional, Glösmann, Martin, additional, Bernhardt, Ricardo, additional, Chopin, Michael, additional, Hempel, Ute, additional, Hofbauer, Lorenz C, additional, Rammelt, Stefan, additional, Egerbacher, Monika, additional, Erben, Reinhold G, additional, and Jessberger, Rolf, additional

Published
2012
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84. Delayed bone regeneration and low bone mass in a rat model of insulin-resistant type 2 diabetes mellitus is due to impaired osteoblast function

Author

Hamann, Christine, primary, Goettsch, Claudia, additional, Mettelsiefen, Jan, additional, Henkenjohann, Veit, additional, Rauner, Martina, additional, Hempel, Ute, additional, Bernhardt, Ricardo, additional, Fratzl-Zelman, Nadja, additional, Roschger, Paul, additional, Rammelt, Stefan, additional, Günther, Klaus-Peter, additional, and Hofbauer, Lorenz C., additional

Published
2011
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