77 results on '"Hoyd, Rebecca"'
Search Results
52. Bayesian structural time series for biomedical sensor data: A flexible modeling framework for evaluating interventions
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Liu, Jason, primary, Spakowicz, Daniel J., additional, Ash, Garrett I., additional, Hoyd, Rebecca, additional, Ahluwalia, Rohan, additional, Zhang, Andrew, additional, Lou, Shaoke, additional, Lee, Donghoon, additional, Zhang, Jing, additional, Presley, Carolyn, additional, Greene, Ann, additional, Stults-Kolehmainen, Matthew, additional, Nally, Laura M., additional, Baker, Julien S., additional, Fucito, Lisa M., additional, Weinzimer, Stuart A., additional, Papachristos, Andrew V., additional, and Gerstein, Mark, additional
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- 2021
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53. Proton pump inhibitor use (PPI) in patients treated with immune checkpoint inhibitors (ICI) for advanced cancer: Survival and prior therapy.
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Husain, Marium, primary, Xu, Menglin, additional, Patel, Sandipkumar, additional, Johns, Andrew, additional, Grogan, Madison, additional, Li, Mingjia, additional, Lopez, Gabrielle, additional, Miah, Abdul, additional, Hoyd, Rebecca, additional, Liu, YunZhou, additional, Muniak, Mitchell, additional, Haddad, Tyler, additional, Tinoco, Gabriel, additional, Kendra, Kari Lynn, additional, Otterson, Gregory Alan, additional, Presley, Carolyn J, additional, Spakowicz, Daniel, additional, and Owen, Dwight Hall, additional
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- 2021
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54. Microbiome signature, global methylation and immune landscape in early onset colorectal cancer.
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Jin, Ning, primary, Mo, Xiaokui, additional, Hoyd, Rebecca, additional, Yilmaz, Ayse Selen, additional, Liu, YunZhou, additional, Jagjit Singh, Malvenderjit, additional, Muniak, Mitchell, additional, Hampel, Heather, additional, and Spakowicz, Daniel, additional
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- 2021
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55. The sarcoma microbiome as a diagnostic and therapeutic target.
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Tinoco, Gabriel, primary, Husain, Marium, additional, Hoyd, Rebecca, additional, Jagjit Singh, Malvenderjit, additional, Liu, YunZhou, additional, Mo, Xiaokui, additional, Chen, James Lin, additional, Liebner, David A., additional, and Spakowicz, Daniel, additional
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- 2021
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56. Investigating intra-tumor microbes, blood microbes, and CEA for development of non-invasive biomarkers in colorectal cancer.
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Malalur, Pannaga G., primary, Mo, Xiaokui, additional, Hoyd, Rebecca, additional, Hays, John L., additional, Carbone, David Paul, additional, and Spakowicz, Daniel, additional
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- 2021
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57. Bayesian Structural Time Series for Mobile Health and Sensor Data: A Flexible Modeling Framework for Evaluating Interventions
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Liu, Jason, Spakowicz, Daniel J., Hoyd, Rebecca, Ash, Garrett I., Lou, Shaoke, Lee, Donghoon, Zhang, Jing, Presley, Carolyn, Greene, Ann, Papachristos, Andrew V, and Gerstein, Mark
- Abstract
The development of mobile health technology has the potential to contribute greatly to personalized medicine. Wearable sensors can assist with determining the proper treatment plans for individuals, provide quantitative information to physicians, or give individuals an objective measurement of their health. However, though treatments and interventions have become more targeted and specific, measuring the causal impact of these actions require more careful considerations of complex covariate structures as well as temporal and spatial properties of the data. Thus, emerging data from sensors and wearables in the near future will make use of and require complex models. Here, we describe a general statistical framework for sensor and wearable data that applies a Bayesian structural time series model to analyze and understand various behavior and health data collected in different environments. We show the wide applicability of this modelling framework, and how it corrects for covariates and biases to provide accurate assessments of intervention. Furthermore, it allows for a time dependent confidence interval of impact through its use of Bayesian estimation. We give three main examples, physical sensor data, environmental air sensors and longitudinal behavioral data to show the effect of various interventions through parameter estimation and comparison in pre- and post-intervention periods. The Bayesian structural time series model shows robust performance in a wide variety of tasks, further supporting its applicability to current and future mobile health and sensor data types.
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- 2020
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58. Additional file 1 of Inferring the role of the microbiome on survival in patients treated with immune checkpoint inhibitors: causal modeling, timing, and classes of concomitant medications
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Spakowicz, Daniel, Hoyd, Rebecca, Muniak, Mitchell, Marium Husain, Bassett, James S., Wang, Lei, Tinoco, Gabriel, Sandip H. Patel, Burkart, Jarred, Miah, Abdul, Mingjia Li, Johns, Andrew, Grogan, Madison, Carbone, David P., Verschraegen, Claire F., Kendra, Kari L., Otterson, Gregory A., Li, Lang, Presley, Carolyn J., and Owen, Dwight H.
- Abstract
Additional file 1: Figure S1. Causal diagram with references. Figure S2. Number of medications prescribed across all cancers and the frequency of multiple medications. Figure S3. Number of antibiotics, separated by class, prescribed across all cancers and the frequency of multiple antibiotics. Figure S4. Number of corticosteroids, separated by class, prescribed across all cancers and the frequency of multiple antibiotics.
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- 2020
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59. Additional file 2 of Inferring the role of the microbiome on survival in patients treated with immune checkpoint inhibitors: causal modeling, timing, and classes of concomitant medications
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Spakowicz, Daniel, Hoyd, Rebecca, Muniak, Mitchell, Marium Husain, Bassett, James S., Wang, Lei, Tinoco, Gabriel, Sandip H. Patel, Burkart, Jarred, Miah, Abdul, Mingjia Li, Johns, Andrew, Grogan, Madison, Carbone, David P., Verschraegen, Claire F., Kendra, Kari L., Otterson, Gregory A., Li, Lang, Presley, Carolyn J., and Owen, Dwight H.
- Abstract
Additional file 2: Table S1. References for the antibiotic susceptibilities of bacterial taxa found to be enriched in responders or non-responders to ICI therapy
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- 2020
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60. Additional file 1 of Approaches for integrating heterogeneous RNA-seq data reveal cross-talk between microbes and genes in asthmatic patients
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Spakowicz, Daniel, Shaoke Lou, Barron, Brian, Gomez, Jose L., Tianxiao Li, Liu, Qing, Grant, Nicole, Xiting Yan, Hoyd, Rebecca, Weinstock, George, Chupp, Geoffrey L., and Gerstein, Mark
- Abstract
Additional file 1:Figures S1-S11. Supplemental Figures.
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- 2020
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61. Abstract 3338: Intra-tumoral microbes correlate with immune cell fractions in lung cancer biopsies not other cancers
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Mo, Xiaokui, primary, Spakowicz, Daniel, additional, and Hoyd, Rebecca, additional
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- 2020
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62. Platelets impact the responsiveness of immune checkpoint blockade therapy in solid tumors.
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Riesenberg, Brian P, primary, Li, Mingjia, additional, Spakowicz, Daniel, additional, Hoyd, Rebecca, additional, Beane, Joal, additional, Yang, Yuanquan, additional, Oezkan, Filiz, additional, He, Kai, additional, Patel, Sandip H., additional, Johns, Andrew, additional, Grogan, Madison, additional, Miah, Abdul, additional, Husain, Marium, additional, Bertino, Erin Marie, additional, Otterson, Gregory Alan, additional, Kendra, Kari Lynn, additional, Presley, Carolyn J, additional, Carbone, David Paul, additional, Li, Zihai, additional, and Owen, Dwight Hall, additional
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- 2020
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63. Intra-tumoral microbes and overall survival in colorectal cancer patients.
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Malalur, Pannaga G., primary, Mo, Xiaokui, additional, Hoyd, Rebecca, additional, Carbone, David Paul, additional, and Spakowicz, Daniel, additional
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- 2020
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64. Abstract B30: Intratumoral microbes correlate with tumor-infiltrating lymphocytes in lung cancer RNAseq
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Spakowicz, Daniel, primary, Hoyd, Rebecca, additional, Liu, YunZhou, additional, Sahasrabudhe, Janhavi, additional, Singh, Malvenderjit J., additional, Arefi, Isaac, additional, Denney, Andrew, additional, Carbone, David, additional, and Mo, Xiaokui, additional
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- 2020
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65. Bayesian Structural Time Series for Biomedical Sensor Data: A Flexible Modeling Framework for Evaluating Interventions
- Author
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Liu, Jason, primary, Spakowicz, Daniel J., additional, Ash, Garrett I., additional, Hoyd, Rebecca, additional, Zhang, Andrew, additional, Lou, Shaoke, additional, Lee, Donghoon, additional, Zhang, Jing, additional, Presley, Carolyn, additional, Greene, Ann, additional, Stults-Kolehmainen, Matthew, additional, Nally, Laura, additional, Baker, Julien S., additional, Fucito, Lisa M., additional, Weinzimer, Stuart A., additional, Papachristos, Andrew V, additional, and Gerstein, Mark, additional
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- 2020
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66. Inferring the role of the microbiome on survival in patients treated with immune checkpoint inhibitors: causal modeling, timing, and classes of concomitant medications
- Author
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Spakowicz, Daniel, primary, Hoyd, Rebecca, additional, Muniak, Mitchell, additional, Husain, Marium, additional, Bassett, James S., additional, Wang, Lei, additional, Tinoco, Gabriel, additional, Patel, Sandip H., additional, Burkart, Jarred, additional, Miah, Abdul, additional, Li, Mingjia, additional, Johns, Andrew, additional, Grogan, Madison, additional, Carbone, David P., additional, Verschraegen, Claire F., additional, Kendra, Kari L., additional, Otterson, Gregory A., additional, Li, Lang, additional, Presley, Carolyn J., additional, and Owen, Dwight H., additional
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- 2019
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67. Is immunotherapy toxicity associated with improved overall survival among older adults with advanced cancer?
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Johns, Andrew, primary, Grogan, Madison, additional, Hoyd, Rebecca, additional, Bridges, John F.P, additional, Wei, Lai, additional, Patel, Sandipkumar, additional, Li, Mingjia, additional, Husain, Marium, additional, Kendra, Kari Lynn, additional, Otterson, Gregory Alan, additional, Burkart, Jarred Thomas, additional, Rosko, Ashley Elizabeth, additional, Andersen, Barbara L., additional, Carbone, David Paul, additional, Owen, Dwight Hall, additional, Spakowicz, Daniel, additional, and Presley, Carolyn J, additional
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- 2019
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68. Re-evaluating the neutrophil-to-lymphocyte ratio: Machine learning-based variable selection for predicting survival at twelve months in late-stage cancer patients receiving immunotherapy.
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Spakowicz, Daniel, primary, Li, Mingjia, additional, Hoyd, Rebecca, additional, Burkart, Jarred Thomas, additional, Patel, Sandip H., additional, Husain, Marium, additional, He, Kai, additional, Presley, Carolyn J, additional, Bertino, Erin Marie, additional, Shields, Peter G., additional, Carbone, David Paul, additional, Shah, Hiral A., additional, Tinoco, Gabriel, additional, Folefac, Edmund, additional, Bhateja, Priyanka, additional, Verschraegen, Claire F., additional, Otterson, Gregory Alan, additional, Li, Lang, additional, Kendra, Kari Lynn, additional, and Owen, Dwight Hall, additional
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- 2019
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69. De-correlating immune checkpoint inhibitor toxicity and response in melanoma via the microbiome.
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Williams, Nyelia, Wheeler, Caroline E., Husain, Marium, Hoyd, Rebecca, Meara, Alexa Simon, Lynn, Mari, Bibi, Amna, Conrad, Bailey, Gray, Shannon, Bodnar, Michael, Arya, Namrata, Roberts, Scott, Hoang, Phuong, Apparicio, Jessica, Merrill, Deanna, Wu, Richard Cheng Han, Verschraegen, Claire F., Burd, Christin Elizabeth, Kendra, Kari Lynn, and Spakowicz, Daniel
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- 2023
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70. An Explainable Graph Neural Framework to Identify Cancer-Associated Intratumoral Microbial Communities.
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Liu Z, Sun Y, Li Y, Ma A, Willaims NF, Jahanbahkshi S, Hoyd R, Wang X, Zhang S, Zhu J, Xu D, Spakowicz D, Ma Q, and Liu B
- Abstract
Microbes are extensively present among various cancer tissues and play critical roles in carcinogenesis and treatment responses. However, the underlying relationships between intratumoral microbes and tumors remain poorly understood. Here, a MIcrobial Cancer-association Analysis using a Heterogeneous graph transformer (MICAH) to identify intratumoral cancer-associated microbial communities is presented. MICAH integrates metabolic and phylogenetic relationships among microbes into a heterogeneous graph representation. It uses a graph transformer to holistically capture relationships between intratumoral microbes and cancer tissues, which improves the explainability of the associations between identified microbial communities and cancers. MICAH is applied to intratumoral bacterial data across 5 cancer types and 5 fungi datasets, and its generalizability and reproducibility are demonstrated. After experimentally testing a representative observation using a mouse model of tumor-microbe-immune interactions, a result consistent with MICAH's identified relationship is observed. Source tracking analysis reveals that the primary known contributor to a cancer-associated microbial community is the organs affected by the type of cancer. Overall, this graph neural network framework refines the number of microbes that can be used for follow-up experimental validation from thousands to tens, thereby helping to accelerate the understanding of the relationship between tumors and intratumoral microbiomes., (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
- Published
- 2024
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71. The Tumor Microbiome as a Predictor of Outcomes in Patients with Metastatic Melanoma Treated with Immune Checkpoint Inhibitors.
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Dravillas CE, Coleman SS 4th, Hoyd R, Caryotakis G, Denko L, Chan CHF, Churchman ML, Denko N, Dodd RD, Eljilany I, Hardikar S, Husain M, Ikeguchi AP, Jin N, Ma Q, McCarter MD, Osman AEG, Robinson LA, Singer EA, Tinoco G, Ulrich CM, Zakharia Y, Spakowicz D, Tarhini AA, and Tan AC
- Subjects
- Humans, Male, Female, Middle Aged, Aged, Adult, Aged, 80 and over, Young Adult, Treatment Outcome, Skin Neoplasms drug therapy, Skin Neoplasms microbiology, Skin Neoplasms immunology, Skin Neoplasms pathology, Neoplasm Metastasis, Prognosis, Melanoma drug therapy, Melanoma microbiology, Melanoma immunology, Melanoma secondary, Immune Checkpoint Inhibitors therapeutic use, Immune Checkpoint Inhibitors pharmacology, Microbiota drug effects
- Abstract
Emerging evidence supports the important role of the tumor microbiome in oncogenesis, cancer immune phenotype, cancer progression, and treatment outcomes in many malignancies. In this study, we investigated the metastatic melanoma tumor microbiome and its potential roles in association with clinical outcomes, such as survival, in patients with metastatic disease treated with immune checkpoint inhibitors (ICI). Baseline tumor samples were collected from 71 patients with metastatic melanoma before treatment with ICIs. Bulk RNA sequencing (RNA-seq) was conducted on the formalin-fixed, paraffin-embedded and fresh frozen tumor samples. Durable clinical benefit (primary clinical endpoint) following ICIs was defined as overall survival >24 months and no change to the primary drug regimen (responders). We processed RNA-seq reads to carefully identify exogenous sequences using the {exotic} tool. The age of the 71 patients with metastatic melanoma ranged from 24 to 83 years, 59% were male, and 55% survived >24 months following the initiation of ICI treatment. Exogenous taxa were identified in the tumor RNA-seq, including bacteria, fungi, and viruses. We found differences in gene expression and microbe abundances in immunotherapy-responsive versus nonresponsive tumors. Responders showed significant enrichment of bacteriophages in the phylum Uroviricota, and nonresponders showed enrichment of several bacteria, including Campylobacter jejuni. These microbes correlated with immune-related gene expression signatures. Finally, we found that models for predicting prolonged survival with immunotherapy using both microbe abundances and gene expression outperformed models using either dataset alone. Our findings warrant further investigation and potentially support therapeutic strategies to modify the tumor microbiome in order to improve treatment outcomes with ICIs., Significance: We analyzed the tumor microbiome and interactions with genes and pathways in metastatic melanoma treated with immunotherapy and identified several microbes associated with immunotherapy response and immune-related gene expression signatures. Machine learning models that combined microbe abundances and gene expression outperformed models using either dataset alone in predicting immunotherapy responses., (©2024 The Authors; Published by the American Association for Cancer Research.)
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- 2024
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72. The Tumor Microbiome Reacts to Hypoxia and Can Influence Response to Radiation Treatment in Colorectal Cancer.
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Benej M, Hoyd R, Kreamer M, Wheeler CE, Grencewicz DJ, Choueiry F, Chan CHF, Zakharia Y, Ma Q, Dodd RD, Ulrich CM, Hardikar S, Churchman ML, Tarhini AA, Robinson LA, Singer EA, Ikeguchi AP, McCarter MD, Tinoco G, Husain M, Jin N, Tan AC, Osman AEG, Eljilany I, Riedlinger G, Schneider BP, Benejova K, Kery M, Papandreou I, Zhu J, Denko N, and Spakowicz D
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- Animals, Mice, Humans, Microbiota radiation effects, Cell Line, Tumor, Female, Colorectal Neoplasms radiotherapy, Colorectal Neoplasms microbiology, Tumor Hypoxia radiation effects, Mice, Inbred BALB C, Mice, Nude
- Abstract
Tumor hypoxia has been shown to predict poor patient outcomes in several cancer types, partially because it reduces radiation's ability to kill cells. We hypothesized that some of the clinical effects of hypoxia could also be due to its impact on the tumor microbiome. Therefore, we examined the RNA sequencing data from the Oncology Research Information Exchange Network database of patients with colorectal cancer treated with radiotherapy. We identified microbial RNAs for each tumor and related them to the hypoxic gene expression scores calculated from host mRNA. Our analysis showed that the hypoxia expression score predicted poor patient outcomes and identified tumors enriched with certain microbes such as Fusobacterium nucleatum. The presence of other microbes, such as Fusobacterium canifelinum, predicted poor patient outcomes, suggesting a potential interaction between hypoxia, the microbiome, and radiation response. To experimentally investigate this concept, we implanted CT26 colorectal cancer cells into immune-competent BALB/c and immune-deficient athymic nude mice. After growth, in which tumors passively acquired microbes from the gastrointestinal tract, we harvested tumors, extracted nucleic acids, and sequenced host and microbial RNAs. We stratified tumors based on their hypoxia score and performed a metatranscriptomic analysis of microbial gene expression. In addition to hypoxia-tropic and -phobic microbial populations, analysis of microbial gene expression at the strain level showed expression differences based on the hypoxia score. Thus, hypoxia gene expression scores seem to associate with different microbial populations and elicit an adaptive transcriptional response in intratumoral microbes, potentially influencing clinical outcomes., Significance: Tumor hypoxia reduces radiotherapy efficacy. In this study, we explored whether some of the clinical effects of hypoxia could be due to interaction with the tumor microbiome. Hypoxic gene expression scores associated with certain microbes and elicited an adaptive transcriptional response in others that could contribute to poor clinical outcomes., (©2024 The Authors; Published by the American Association for Cancer Research.)
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- 2024
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73. Dynamic Human Gut Microbiome and Immune Shifts During an Immersive Psychosocial Therapeutic Program.
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Zhou X, Ganz AB, Rayner A, Cheng TY, Oba H, Rolnik B, Lancaster S, Lu X, Li Y, Johnson JS, Hoyd R, Spakowicz DJ, Slavich GM, and Snyder MP
- Abstract
Background: Depression is a leading cause of disability worldwide yet its underlying factors, particularly microbial associations, are poorly understood., Methods: We examined the longitudinal interplay between the microbiome and immune system in the context of depression during an immersive psychosocial intervention. 142 multi-omics samples were collected from 52 well-characterized participants before, during, and three months after a nine-day inquiry-based stress reduction program., Results: We found that depression was associated with both an increased presence of putatively pathogenic bacteria and reduced microbial beta-diversity. Following the intervention, we observed reductions in neuroinflammatory cytokines and improvements in several mental health indicators. Interestingly, participants with a Prevotella -dominant microbiome showed milder symptoms when depressed, along with a more resilient microbiome and more favorable inflammatory cytokine profile, including reduced levels of CXCL-1., Conclusions: Our findings reveal a protective link between the Prevotella-dominant microbiome and depression, associated with a less inflammatory environment and moderated symptoms. These insights, coupled with observed improvements in neuroinflammatory markers and mental health from the intervention, highlight potential avenues for microbiome-targeted therapies in depression management., Competing Interests: CONFLICT OF INTEREST M.P.S. is a co-founder and the scientific advisory board member of Personalis, Qbio, January, SensOmics, Filtricine, Akna, Protos, Mirvie, NiMo, Onza, Oralome, Marble Therapeutics, and Iollo. He is also on the scientific advisory board of Danaher, Genapsys, and Jupiter. A. B. G. is a founding partner at Arben Ventures and Xthena Partners. The fund she manages through Arben Ventures is an advisor to Elemind Technologies, Northstar Care, and Bloch Quantum Imaging. These organizations had no role in planning, writing, editing, or reviewing this article, or in deciding to submit this article for publication. All other authors report no biomedical financial interests or potential conflicts of interest.
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- 2024
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74. Exogenous Sequences in Tumors and Immune Cells (Exotic): A Tool for Estimating the Microbe Abundances in Tumor RNA-seq Data.
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Hoyd R, Wheeler CE, Liu Y, Jagjit Singh MS, Muniak M, Jin N, Denko NC, Carbone DP, Mo X, and Spakowicz DJ
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- Humans, RNA-Seq, Sequence Analysis, RNA, RNA, Neoplasm, Neoplasms genetics, Microbiota genetics
- Abstract
The microbiome affects cancer, from carcinogenesis to response to treatments. New evidence suggests that microbes are also present in many tumors, though the scope of how they affect tumor biology and clinical outcomes is in its early stages. A broad survey of tumor microbiome samples across several independent datasets is needed to identify robust correlations for follow-up testing. We created a tool called {exotic} for "exogenous sequences in tumors and immune cells" to carefully identify the tumor microbiome within RNA sequencing (RNA-seq) datasets. We applied it to samples collected through the Oncology Research Information Exchange Network (ORIEN) and The Cancer Genome Atlas. We showed how the processing removes contaminants and batch effects to yield microbe abundances consistent with non-high-throughput sequencing-based approaches and DNA-amplicon-based measurements of a subset of the same tumors. We sought to establish clinical relevance by correlating the microbe abundances with various clinical and tumor measurements, such as age and tumor hypoxia. This process leveraged the two datasets and raised up only the concordant (significant and in the same direction) associations. We observed associations with survival and clinical variables that are cancer specific and relatively few associations with immune composition. Finally, we explored potential mechanisms by which microbes and tumors may interact using a network-based approach. Alistipes, a common gut commensal, showed the highest network degree centrality and was associated with genes related to metabolism and inflammation. The {exotic} tool can support the discovery of microbes in tumors in a way that leverages the many existing and growing RNA-seq datasets., Significance: The intrinsic tumor microbiome holds great potential for its ability to predict various aspects of cancer biology and as a target for rational manipulation. Here, we describe a tool to quantify microbes from within tumor RNA-seq and apply it to two independent datasets. We show new associations with clinical variables that justify biomarker uses and more experimentation into the mechanisms by which tumor microbiomes affect cancer outcomes., (© 2023 The Authors; Published by the American Association for Cancer Research.)
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- 2023
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75. Improving combination therapies: targeting A2B-adenosine receptor to modulate metabolic tumor microenvironment and immunosuppression.
- Author
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Evans JV, Suman S, Goruganthu MUL, Tchekneva EE, Guan S, Arasada RR, Antonucci A, Piao L, Ilgisonis I, Bobko AA, Driesschaert B, Uzhachenko RV, Hoyd R, Samouilov A, Amann J, Wu R, Wei L, Pallerla A, Ryzhov SV, Feoktistov I, Park KP, Kikuchi T, Castro J, Ivanova AV, Kanagasabai T, Owen DH, Spakowicz DJ, Zweier JL, Carbone DP, Novitskiy SV, Khramtsov VV, Shanker A, and Dikov MM
- Subjects
- Humans, Animals, Mice, Receptor, Adenosine A2B metabolism, Tumor Microenvironment, Immunosuppression Therapy, Adenosine metabolism, Phosphates, Cell Line, Tumor, Carcinoma, Non-Small-Cell Lung drug therapy, Lung Neoplasms drug therapy, Lung Neoplasms pathology
- Abstract
Background: We investigated the role of A2B-adenosine receptor in regulating immunosuppressive metabolic stress in the tumor microenvironment. Novel A2B-adenosine receptor antagonist PBF-1129 was tested for antitumor activity in mice and evaluated for safety and immunologic efficacy in a phase I clinical trial of patients with non-small cell lung cancer., Methods: The antitumor efficacy of A2B-adenosine receptor antagonists and their impact on the metabolic and immune tumor microenvironment were evaluated in lung, melanoma, colon, breast, and epidermal growth factor receptor-inducible transgenic cancer models. Employing electron paramagnetic resonance, we assessed changes in tumor microenvironment metabolic parameters, including pO2, pH, and inorganic phosphate, during tumor growth and evaluated the immunologic effects of PBF-1129, including its pharmacokinetics, safety, and toxicity, in patients with non-small cell lung cancer., Results: Levels of metabolic stress correlated with tumor growth, metastasis, and immunosuppression. Tumor interstitial inorganic phosphate emerged as a correlative and cumulative measure of tumor microenvironment stress and immunosuppression. A2B-adenosine receptor inhibition alleviated metabolic stress, downregulated expression of adenosine-generating ectonucleotidases, increased expression of adenosine deaminase, decreased tumor growth and metastasis, increased interferon γ production, and enhanced the efficacy of antitumor therapies following combination regimens in animal models (anti-programmed cell death 1 protein vs anti-programmed cell death 1 protein plus PBF-1129 treatment hazard ratio = 11.74 [95% confidence interval = 3.35 to 41.13], n = 10, P < .001, 2-sided F test). In patients with non-small cell lung cancer, PBF-1129 was well tolerated, with no dose-limiting toxicities; demonstrated pharmacologic efficacy; modulated the adenosine generation system; and improved antitumor immunity., Conclusions: Data identify A2B-adenosine receptor as a valuable therapeutic target to modify metabolic and immune tumor microenvironment to reduce immunosuppression, enhance the efficacy of immunotherapies, and support clinical application of PBF-1129 in combination therapies., (© The Author(s) 2023. Published by Oxford University Press.)
- Published
- 2023
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76. The tumor microbiome as a predictor of outcomes in patients with metastatic melanoma treated with immune checkpoint inhibitors.
- Author
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Wheeler CE, Coleman SS 4th, Hoyd R, Denko L, Chan CHF, Churchman ML, Denko N, Dodd RD, Eljilany I, Hardikar S, Husain M, Ikeguchi AP, Jin N, Ma Q, McCarter MD, Osman AEG, Robinson LA, Singer EA, Tinoco G, Ulrich CM, Zakharia Y, Spakowicz D, Tarhini AA, and Tan AC
- Abstract
Emerging evidence supports the important role of the tumor microbiome in oncogenesis, cancer immune phenotype, cancer progression, and treatment outcomes in many malignancies. In this study, we investigated the metastatic melanoma tumor microbiome and potential roles in association with clinical outcomes, such as survival, in patients with metastatic disease treated with immune checkpoint inhibitors (ICIs). Baseline tumor samples were collected from 71 patients with metastatic melanoma before treatment with ICIs. Bulk RNA-seq was conducted on the formalin-fixed paraffin-embedded (FFPE) tumor samples. Durable clinical benefit (primary clinical endpoint) following ICIs was defined as overall survival ≥24 months and no change to the primary drug regimen (responders). We processed RNA-seq reads to carefully identify exogenous sequences using the {exotic} tool. The 71 patients with metastatic melanoma ranged in age from 24 to 83 years, 59% were male, and 55% survived >24 months following the initiation of ICI treatment. Exogenous taxa were identified in the tumor RNA-seq, including bacteria, fungi, and viruses. We found differences in gene expression and microbe abundances in immunotherapy responsive versus non-responsive tumors. Responders showed significant enrichment of several microbes including Fusobacterium nucleatum, and non-responders showed enrichment of fungi, as well as several bacteria. These microbes correlated with immune-related gene expression signatures. Finally, we found that models for predicting prolonged survival with immunotherapy using both microbe abundances and gene expression outperformed models using either dataset alone. Our findings warrant further investigation and potentially support therapeutic strategies to modify the tumor microbiome in order to improve treatment outcomes with ICIs., Competing Interests: Conflicts of Interest AAT: Contracted research grants with institution from Bristol Myers Squib, Genentech-Roche, Regeneron, Sanofi-Genzyme, Nektar, Clinigen, Merck, Acrotech, Pfizer, Checkmate, OncoSec. Personal consultant/advisory board fees from Bristol Myers Squibb, Merck, Easai, Instil Bio Clinigin, Regeneron, Sanofi-Genzyme, Novartis, Partner Therapeutics, Genentech/Roche, BioNTech, Concert AI, AstraZeneca outside the submitted work. EAS: Astellas/Medivation: research support (clinical trial); Johnson & Johnson: advisory board; Merck: advisory board; Vyriad: advisory board; Aura Biosciences: data safety monitoring board CC: None related to this project. Other unrelated projects and clinical trials (research support from Checkmate Pharmaceuticals, Regeneron, Angiodynamics, Optimum Therapeutics) YZ: Advisory Board: Bristol Myers Squibb, Amgen, Roche Diagnostics, Novartis, Janssen, Eisai, Exelixis, Castle Bioscience, Genzyme Corporation, Astrazeneca, Array, Bayer, Pfizer, Clovis, EMD Serono, Myovant. Grant/research support from: Institution clinical trial support from NewLink Genetics, Pfizer, Exelixis, Eisai. DSMC: Janssen Research and Development Consultant honorarium: Pfizer, Novartis JC: Roche/Genentech CEW, SC, RH, LD, MC, ND, RDD, SH, MH, API, NJ, QM, MM, AEGO, LAR, GT, CMU, DS, ACT: None to report
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- 2023
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77. A bioinformatics tool for identifying intratumoral microbes from the ORIEN dataset.
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Wang C, Ma A, McNutt ME, Hoyd R, Wheeler CE, Robinson LA, Chan CHF, Zakharia Y, Dodd RD, Ulrich CM, Hardikar S, Churchman ML, Tarhini AA, Singer EA, Ikeguchi AP, McCarter MD, Denko N, Tinoco G, Husain M, Jin N, Osman AEG, Eljilany I, Tan AC, Coleman SS 4th, Denko L, Riedlinger G, Schneider BP, Spakowicz D, and Ma Q
- Abstract
Evidence supports significant interactions among microbes, immune cells, and tumor cells in at least 10-20% of human cancers, emphasizing the importance of further investigating these complex relationships. However, the implications and significance of tumor-related microbes remain largely unknown. Studies have demonstrated the critical roles of host microbes in cancer prevention and treatment responses. Understanding interactions between host microbes and cancer can drive cancer diagnosis and microbial therapeutics (bugs as drugs). Computational identification of cancer-specific microbes and their associations is still challenging due to the high dimensionality and high sparsity of intratumoral microbiome data, which requires large datasets containing sufficient event observations to identify relationships, and the interactions within microbial communities, the heterogeneity in microbial composition, and other confounding effects that can lead to spurious associations. To solve these issues, we present a bioinformatics tool, MEGA, to identify the microbes most strongly associated with 12 cancer types. We demonstrate its utility on a dataset from a consortium of 9 cancer centers in the Oncology Research Information Exchange Network (ORIEN). This package has 3 unique features: species-sample relations are represented in a heterogeneous graph and learned by a graph attention network; it incorporates metabolic and phylogenetic information to reflect intricate relationships within microbial communities; and it provides multiple functionalities for association interpretations and visualizations. We analyzed 2704 tumor RNA-seq samples and MEGA interpreted the tissue-resident microbial signatures of each of 12 cancer types. MEGA can effectively identify cancer-associated microbial signatures and refine their interactions with tumors., Competing Interests: CONFLICTS OF INTEREST Carlos Chan: None related to this project. Other unrelated projects and clinical trials (Research support from Checkmate Pharmaceuticals, Regeneron, Angiodynamics, Optimum Therapeutics) Yousef Zakharia: Advisory Board: Bristol Myers Squibb, Amgen, Roche Diagnostics, Novartis, Janssen, Eisai, Exelixis, Castle Bioscience, Genzyme Corporation, Astrazeneca, Array, Bayer, Pfizer, Clovis, EMD serono, Myovant. Grant/research support from: Institution clinical trial support from NewLink Genetics, Pfizer, Exelixis, Eisai. DSMC: Janssen Research and Development Consultant honorarium: Pfizer, Novartis Ahmad Tarhini: Contracted research grants with institution from Bristol Myers Squib, Genentech-Roche, Regeneron, Sanofi-Genzyme, Nektar, Clinigen, Merck, Acrotech, Pfizer, Checkmate, OncoSec. Personal consultant/advisory board fees from Bristol Myers Squibb, Merck, Easai, Instil Bio Clinigin, Regeneron, Sanofi-Genzyme, Novartis, Partner Therapeutics, Genentech/Roche, BioNTech, Concert AI, AstraZeneca outside the submitted work. Eric Singer: Astellas/Medivation: research support (clinical trial); Johnson & Johnson: advisory board; Merck: advisory board; Vyriad: advisory board; Aura Biosciences: data safety monitoring board Gregory Riedlinger: AstraZeneca advisory board Bryan Schneider: Genentech-Research support (drug supply only); Pfizer-Research support (Drug supply only); Foundation Medicine-research support (sequencing support)
- Published
- 2023
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