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Objective To investigate the integration characteristics of hepatitis B virus (HBV) viral DNA in the host genome of hepatocellular carcinoma HepG2.2.15 cells and HepG2-derived cell line HepAD38 by probe-based capture and high-throughput sequencing technology. Methods After the genomic DNA of HepG2.2.15 and HepAD38 cells were extracted, probe-based capture technique was used to capture the integrated DNA fragments of HBV DNA in the obtained genomic sequences, and the intergrated fragments were further studied with high-throughput sequencing technology. The bioinformatics software such as Trimmatic, BBAP, and BWA (Burrows-Wheeler Aligner) were empolyed for data analysis. Finally, the characteristics of integrated DNA were validated through PCR and cloning and sequencing. Results The probe-based capture and high-throughput sequencing technology detected 12 and 7 integrated DNA fragments in HepG2.2.15 and HepAD38 cells, respectively. These integration events were randomly distributed on chromosomes chr1, chr2, chr7, chr9, chr16, chr17, chr19, and chrX. DPP7, TRIM56 and GPC3 appeared with higher frequencies at the upstream and downstream of the integration sites. Conclusion We successfully establish a method to detect and analyze the integrated DNA fragments of HBV DNA in host genome based on probe-based capture and high-throughput sequencing technology. HBV integration events occur randomly on the chromosomes in HepG2.2.15 and HepAD38 cells, and most of integration sites are in the X gene region of the HBV genome.

[Purpose/Significance] When emergencies occur, the information environment becomes complex, users' emotions are strongly affected, and the social mentality fluctuates. This situation also poses new requirements for the management of network public opinion. It is urgent to actively and correctly manage the current social mentality. The research on the current social mentality mainly focuses on the macro level, while the micro-user emotion and the macro-network social mentality need to be further explored. Therefore, it is necessary to explore the fine-grained influencing factors of the network social mentality in public emergencies, so as to promote the effective response to public emergencies in China and manage social mentality. [Method/Process] The meta-synthesis method is used to formulate a reasonable retrieval strategy. Based on the mainstream databases at home and abroad, the literature is screened by topic, and the quality of related topic literature is evaluated to obtain effective literature and use it as the original data. Through the form of three-level coding, the included literature was interpreted, translated and aggregated to test the reliability and validity of the research results and confirm the validity of the results. This paper summarizes the key variables and implicit relations of the existing research results, and constructs a comprehensive and micro-theoretical framework of the influencing factors of network social mentality in public emergencies. The relationship between the dimensions and the main categories in the model was analyzed. [Results/Conclusions] The study finally obtained 90 initial concepts, 31 categories and 13 main categories, which were summarized into a model of influencing factors composed of four dimensions: user, event, government and social environment. As the main body and direct cause of the change of network social mentality, users and events are the core objects of network social mentality management. As the external environment for the change of network social mentality, society and government play an important role in the management and standardization of network social mentality. The theoretical model framework can provide some guidance and reference for promoting public emergency response and social mentality management. It is also of great significance for the study of network social mentality and user emotion. There are still some shortcomings in the article. The research object of this paper has not yet been more official and unified. The definition is a self-summary based on literature reading. In addition, due to the specificity of the research object, there may be a problem of incomplete literature search. In the future research, the management model will be further explored in combination with the development of practice, and the model framework will be revised and expanded.

Abstract Quantum‐based technologies will provide system engineers with new capabilities for securing data communications. The UK AirQKD project has implemented a Free‐Space Optical Quantum Key Distribution (QKD) system to enable the continuous generation of symmetric encryption keys. One of the use cases for the generated keys is to secure Vehicle‐to‐Everything (V2X) communications. V2X applications would benefit from the certificate‐free security provided by QKD for a post‐quantum society. How FSO‐QKD could integrate into a V2X architecture is examined. An overview of V2X is provided with the role that FSO‐QKD could secure V2X data though some obstacles exist. One of the issues with 6G communications is the potential line‐of‐sight (LOS) considerations between the V2X devices. The modelling required for LOS is examined to analyse the outage performance of the building to infrastructure links in the 6G architecture. The results from the model show that further work is required if 6G LOS communications are going to be relied upon for future safety‐critical V2X applications.