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1,358 results on '"Huang, Qiaoyun"'

51. Geologically younger ecosystems are more dependent on soil biodiversity for supporting function

Author

National Key Research and Development Program (China), National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities (China), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), European Commission, Junta de Andalucía, Feng, Jiao [0000-0003-2879-3498], Liu, Yurong [0000-0003-1112-4255], Eldridge, David J. [0000-0002-2191-486X], Huang, Qiaoyun [0000-0002-2733-8066], Tan, Wenfeng [0000-0002-3098-2928], Delgado-Baquerizo, Manuel [0000-0002-6499-576X], Feng, Jiao, Liu, Yurong, Eldridge, David J., Huang, Qiaoyun, Tan, Wenfeng, Delgado-Baquerizo, Manuel, National Key Research and Development Program (China), National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities (China), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), European Commission, Junta de Andalucía, Feng, Jiao [0000-0003-2879-3498], Liu, Yurong [0000-0003-1112-4255], Eldridge, David J. [0000-0002-2191-486X], Huang, Qiaoyun [0000-0002-2733-8066], Tan, Wenfeng [0000-0002-3098-2928], Delgado-Baquerizo, Manuel [0000-0002-6499-576X], Feng, Jiao, Liu, Yurong, Eldridge, David J., Huang, Qiaoyun, Tan, Wenfeng, and Delgado-Baquerizo, Manuel

Abstract

Soil biodiversity contains the metabolic toolbox supporting organic matter decomposition and nutrient cycling in the soil. However, as soil develops over millions of years, the buildup of plant cover, soil carbon and microbial biomass may relax the dependence of soil functions on soil biodiversity. To test this hypothesis, we evaluate the within-site soil biodiversity and function relationships across 87 globally distributed ecosystems ranging in soil age from centuries to millennia. We found that within-site soil biodiversity and function relationship is negatively correlated with soil age, suggesting a stronger dependence of ecosystem functioning on soil biodiversity in geologically younger than older ecosystems. We further show that increases in plant cover, soil carbon and microbial biomass as ecosystems develop, particularly in wetter conditions, lessen the critical need of soil biodiversity to sustain function. Our work highlights the importance of soil biodiversity for supporting function in drier and geologically younger ecosystems with low microbial biomass. © The Author(s) 2024.

Published
2024

52. Metabolic coupling between soil aerobic methanotrophs and denitrifiers in rice paddy fields

Author

National Key Research and Development Program (China), National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities (China), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Junta de Andalucía, European Commission, Feng, Jiao [0000-0003-2879-3498], Yang, Ziming [0000-0003-0312-8408], Huang, Qiaoyun [0000-0002-2733-8066], Delgado-Baquerizo, Manuel [0000-0002-6499-576X], Cai, Peng [0000-0002-3103-1542], Tang, Wenfeng [0000-0002-3098-2928], Liu, Yu-Rong [0000-0003-1112-4255], Chen, Kang-Hua, Feng, Jiao, Bodelier, Paul L. E., Yang, Ziming, Huang, Qiaoyun, Delgado-Baquerizo, Manuel, Cai, Peng, Tang, Wenfeng, Liu, Yu-Rong, National Key Research and Development Program (China), National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities (China), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Junta de Andalucía, European Commission, Feng, Jiao [0000-0003-2879-3498], Yang, Ziming [0000-0003-0312-8408], Huang, Qiaoyun [0000-0002-2733-8066], Delgado-Baquerizo, Manuel [0000-0002-6499-576X], Cai, Peng [0000-0002-3103-1542], Tang, Wenfeng [0000-0002-3098-2928], Liu, Yu-Rong [0000-0003-1112-4255], Chen, Kang-Hua, Feng, Jiao, Bodelier, Paul L. E., Yang, Ziming, Huang, Qiaoyun, Delgado-Baquerizo, Manuel, Cai, Peng, Tang, Wenfeng, and Liu, Yu-Rong

Abstract

Paddy fields are hotspots of microbial denitrification, which is typically linked to the oxidation of electron donors such as methane (CH4) under anoxic and hypoxic conditions. While several anaerobic methanotrophs can facilitate denitrification intracellularly, whether and how aerobic CH4 oxidation couples with denitrification in hypoxic paddy fields remains virtually unknown. Here we combine a ~3300 km field study across main rice-producing areas of China and 13CH4-DNA-stable isotope probing (SIP) experiments to investigate the role of soil aerobic CH4 oxidation in supporting denitrification. Our results reveal positive relationships between CH4 oxidation and denitrification activities and genes across various climatic regions. Microcosm experiments confirm that CH4 and methanotroph addition promote gene expression involved in denitrification and increase nitrous oxide emissions. Moreover, 13CH4-DNA-SIP analyses identify over 70 phylotypes harboring genes associated with denitrification and assimilating 13C, which are mostly belonged to Rubrivivax, Magnetospirillum, and Bradyrhizobium. Combined analyses of 13C-metagenome-assembled genomes and 13C-metabolomics highlight the importance of intermediates such as acetate, propionate and lactate, released during aerobic CH4 oxidation, for the coupling of CH4 oxidation with denitrification. Our work identifies key microbial taxa and pathways driving coupled aerobic CH4 oxidation and denitrification, with important implications for nitrogen management and greenhouse gas regulation in agroecosystems.

Published
2024

57. Impact of endometrial thickness and its combined effect with maternal age on singleton adverse neonatal outcomes in frozen–thawed embryo transfer cycles.

Author

Wang, Jie, Gao, Lina, Huang, Qiaoyun, Jiang, Weihua, Chen, Linjun, and Wang, Shanshan

Subjects
LOW birth weight, MATERNAL age, EMBRYO transfer, GESTATIONAL age, PREMATURE labor, FROZEN human embryos
Abstract

Background: Thin endometrial thickness (EMT) and advanced age are both common risk factors for adverse neonatal outcomes (ANOs). However, studies evaluating the impact of EMT and combined effect of EMT and age on ANOs remain scarce with conflicts. Method: A retrospective cohort study was conducted on 7,715 singleton deliveries from frozen embryo transfer (FET) cycles between 2017 and 2021. The participants were categorized into four groups based on EMT quartiles (≤8.5, 8.6–9.5, 9.6–10.5, and >10.5 mm). The primary outcome was preterm birth (PTB), and the secondary outcome included early PTB (EPTB), small/large for gestational age (SGA/LGA), and low birth weight (LBW). Results: Baseline characteristics were unevenly distributed across EMT groups. After adjusting for the confounders, EMT was found to be associated with the incidence of PTB (aOR 0.922, 95% CI 0.874–0.973, p = 0.003), EPTB (aOR 0.795, 95% CI 0.663–0.954, p = 0.014), LBW (aOR 0.886, 95% CI 0.796–0.986, p = 0.027), and LGA (aOR 1.038, 95% CI 1.004–1.074, p = 0.030). Furthermore, the rates of LBW in the group of EMT at 9.6–10.5 mm (aOR 0.551, 95% CI 0.339–0.895, p = 0.016) and >10.5 mm (aOR 0.536, 95% CI 0.332–0.865, p = 0.011) were lower compared to those with EMT ≤8.5 mm. Among women aged over 35, EMT of 9.6–10.5 mm was associated with a significantly lower incidence of LBW compared to thinner EMT, without increasing the risk of ANOs related to thicker EMT. Conclusions: Our study demonstrated the independent nonlinear impact of EMT on PTB, EPTB, LGA, and LBW. It provided new insights into the combined effects of EMT and age in FET cycles and offered valuable references for the clinical management and treatment strategies aimed at EMT. [ABSTRACT FROM AUTHOR]

Published
2025
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66. Impact of metal oxide nanoparticles on in vitro DNA amplification

Author

Gao, Chun-Hui, Mortimer, Monika, Zhang, Ming, Holden, Patricia A, Cai, Peng, Wu, Shan, Xin, Yuexing, Wu, Yichao, and Huang, Qiaoyun

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Genetics, Bioengineering, Nanotechnology, Nanoparticle, DNA polymerase, Metal oxides, DNA replication, Genotoxiciy, Genotoxicity, Biological Sciences, Medical and Health Sciences
Abstract

Polymerase chain reaction (PCR) is used as an in vitro model system of DNA replication to assess the genotoxicity of nanoparticles (NPs). Prior results showed that several types of NPs inhibited PCR efficiency and increased amplicon error frequency. In this study, we examined the effects of various metal oxide NPs on inhibiting PCR, using high- vs. low-fidelity DNA polymerases; we also examined NP-induced DNA mutation bias at the single nucleotide level. The effects of seven major types of metal oxide NPs (Fe2O3, ZnO, CeO2, Fe3O4, Al2O3, CuO, and TiO2) on PCR replication via a low-fidelity DNA polymerase (Ex Taq) and a high-fidelity DNA polymerase (Phusion) were tested. The successfully amplified PCR products were subsequently sequenced using high-throughput amplicon sequencing. Using consistent proportions of NPs and DNA, we found that the effects of NPs on PCR yield differed depending on the DNA polymerase. Specifically, the efficiency of the high-fidelity DNA polymerase (Phusion) was significantly inhibited by NPs during PCR; such inhibition was not evident in reactions with Ex Taq. Amplicon sequencing showed that the overall error rate of NP-amended PCR was not significantly different from that of PCR without NPs (p > 0.05), and NPs did not introduce single nucleotide polymorphisms during PCR. Thus, overall, NPs inhibited PCR amplification in a DNA polymerase-specific manner, but mutations were not introduced in the process.

Published
2019

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