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The synthesis of G- N 2 -(CH 2 ) 3 -G trimethylene DNA interstrand cross-links (ICLs) in a 5'-CG-3' and 5'-GC-3' sequence from oligodeoxynucleotides containing N 2 -G trimethylene DNA interstrand cross-links (ICLs) in a 5'-CG-3' and 5'-GC-3' sequence from oligodeoxynucleotides containing N 2 -(3-aminopropyl)-2'-deoxyguanosine and 2-fluoro- O 6 -(trimethylsilylethyl)inosine is presented. Automated solid-phase DNA synthesis was used for unmodified bases and modified nucleotides were incorporated via their corresponding phosphoramidite reagent by a manual coupling protocol. The preparation of the phosphoramidite reagents for incorporation of N 2 -(3-aminopropyl)-2'-deoxyguanosine is reported. The high-purity trimethylene DNA interstrand cross-link product is obtained through a nucleophilic aromatic substitution reaction between the N 2 -(3-aminopropyl)-2'-deoxyguanosine and 2-fluoro- O 6 -(trimethylsilylethyl)inosine containing oligodeoxynucleotides.

We performed a case-control study to assess the relationship between six single nucleotide polymorphisms (SNPs) of xeroderma pigmentosum complementation group F (XPF) on glioma risk in a Chinese population. The six SNPs were genotyped in 330 glioma cases and 652 cancer-free controls using a 384-well plate format on the Sequenom MassARRAY platform (Sequenom, San Diego, USA). Rs1800067 did not follow the Hardy-Weinberg equilibrium in the control group, and the genotype distributions differed significantly between the two groups for SNPs rs1800067 and rs2276466. For rs1800067, the variant genotype T/T was strongly associated with an increased risk of glioma when compared with the A/A genotype (OR=3.77, 95% CI=2.38-6.01). Individuals with the rs1800067 G allele had a relatively high risk of glioma in a dominant model (OR=3.47, 95% CI=2.26-5.37). The rs2276466 G/G genotype was significantly associated with a moderate increased risk of glioma (OR=1.82, 95% CI=1.10-3.02) in a codominant model, and variation of rs25489 was associated with a 1.31- and 1.78-fold glioma risk in dominant and recessive models, respectively. Our study is the first to identify polymorphisms in rs1800067 and rs2276466 as correlated with glioma susceptibility., (© 2013 Elsevier B.V. All rights reserved.)