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In this study, the mitochondrial genome of Melanotaenia boesemani (Allen & Cross, 1980 ) (Atheriniformes: Melanotaeniidae) was sequenced for the first time. The assembled mitogenome consisting of 16 493 bp, includes 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and 1 putative control region. The overall base composition of M. boesemani is 27.88% for A, 29.92% for C, 15.74% for G, 26.47% for T and shows 96% identities to Lake Kutubu rainbowfish, Melanotaenia lacustris. These data would provide useful molecular information for phylogenetic relationships within the family Melanotaeniidae species.

In this study, the complete mitogenome sequence of the Parkinson's Rainbowfish, Melanotaenia parkinsoni (Atheriniformes: Melanotaeniidae) has been sequenced by next-generation sequencing method. The assembled mitogenome is 16 529 bp in length including 13 protein-coding genes, 22 transfer RNAs and 2 ribosomal RNAs genes. The overall base composition of Parkinson's Rainbowfish is 28.1% A, 29.7% C, 15.7% G, 26.5% T and shows 93% identity with Lake Kutubu Rainbowfish, M. lacustris. The complete mitogenome of the Parkinson's Rainbowfish provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for Rainbowfish phylogeny.

The midas cichlid (Amphilophus citrinellus) is an important aquarium fish that has served as a model organism for studying sympatric speciation. In this study, we sequenced the complete mitochondrial genome of the midas cichlid. We report that the cichlid’s mitochondrial genome is a circular DNA double strand of 16,521 bp length, which contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. The overall-base compositions of the H-strand are as follows: A, 28.56%; C, 30.69%; G, 15.11%; T, 25.64%. This study provides important genomic data to further the research of the genetic evolution of cichlids.

This paper focuses on China Carbon Sequestration Market (CSM) and establishes the reliability assessment index frame of Carbon Sequestration Market simulation. It draws the conclusion that the CSM simulation is “More reliable” totally and the multi-agent dynamic gaming simulation of forest sequestration market is reliable and necessary. But these results also mean that this research exist some flaws to some extent. So more testify is needed in the aspect of simulation necessities. In terms of simulation condition, we also should enhance the construction of research basis and improve the quality of researchers continuously. It lays down the foundation for future study on China Sequestration Market.

A high g accelerometer based on Silicon-On-Insulator (SOI) piezoresistive material with cantilever beam is described, which can measure the acceleration up to 105 g. The excellent electrical and mechanical properties of SOI material may be suitable in the applications of the high-g force accelerometer as an electromechanical sensor. One kind of SOI Wheatstone bridge strain silicon gauge is developed, and the size of the SOI silicon gauge is 1.8mm×1.6mm×0.2mm. A kind of cantilever beam structure is designed to endure the impact of high-g acceleration. The SOI silicon gauge is bonded on the cantilever beam by the way of AuSn eutectic. The fabricated devices have been subjected to shock tests up to 105 g by using a Hopkinson’s bar, the resonance frequency of which may be up to 40 kHz. The design modeling procedures and results are reported and the fabrication steps are described.Copyright © 2007 by ASME

A method has been developed for the determination of selenomethionine in selenium-enriched yeast by gas chromatography-mass spectrometry (GC-MS). Three extraction methods were compared for extraction efficiency of selenomethionine from the samples. Selenomethionine in the samples was extracted for 24 h with proteinase in Tris buffer. The selenomethionine was derivatized with butanol and trifluoroacetic acid (TFA). The derivatization was accomplished in two steps, starting with the esterification of the carboxyl group of the selenoamino acid using butanol, followed by the acylation of the amino group with trifluoroacetic acid anhydride. The selected ion for monitoring selenomethionine was at m/z 349. The instrument operating conditions were optimized. The samples were analyzed by GC-MS with external standard method. Standard GC-MS chromatograms and mass spectra for selenomethionine were also obtained. The method was proved to be accurate and reliable. The recoveries of 98.5%-103.7% with relative standard deviations (RSDs) of 0.9%-2.4% (n = 6) and the correlation coefficient of 0.9978 were obtained. The detection limit of selenomethionine for the method was 0.5 mg/L (S/N = 3) and the selenomethionine contents of real sample was given.

We performed an investigation of 50 accessory auricles on subjects encountered during 1983 through 1985. Fifteen of the families were found to have the same accessory auricular deformity among the probands' family members. From these 15 families, we selected seven pedigrees that were not accompanied by auricular deformities who had filial generations for analysis. Pedigrees 1 through 6 showed autosomal dominant inheritance; two also had irregular dominant properties (pedigrees 4 and 7), and one (pedigree 7) could not be excluded from the possibility of having an X-linked recessive inheritance. During the investigation, there were latent accessory auricles in which the cartilages were seen to be subcutaneous only or protruding slightly. Genetically, the pathogenesis of an accessory auricle should be related to the accessory auricular gene.