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Dicyanomethylene-4H-benzopyran derivatives dyes containing D- π -A-type structure have been prepared and fully characterized. The Z configuration of all three structures has been confirmed by SCXRD studies. Dyes 1–6 present a strong absorption in the 450–550 nm region with ε values over 43 000 M−1 cm−1 1–4 showed fluorescence properties while 5 and 6 are very weakly fluorescent. The electronic structure of the first singlet excited state S 1 was investigated to further explain the evolution of fluorescence quantum yields. The two-photon excitation spectra were recorded with a similar behaviour upon two-photon excitation, with two absorption bands, one in the 800–850 nm region with σ 2 of 200–300 GM and another one at about 1000 nm with σ 2 of 300–400 GM. Dyes 3 and 4 have shown interesting properties (both in intensity and FLIM modes) in two-photon excited microscopy. [Display omitted] • Push-pull conjugated chromene-derivatives have been synthesized and characterized. • The trans conformation of all three structures has been confirmed by SCXRD studies. • Two of the compounds show interesting properties in two-photon excited microscopy. [ABSTRACT FROM AUTHOR]

Background: Urinary incontinence is a common and distressing condition affecting women worldwide. However, urinary incontinence during pregnancy was less studied. The study aims to investigate the prevalence and risk factors of urinary incontinence during pregnancy, its impact on health-related quality of life as well as associated help-seeking behavior.Methods: Eligible women were enrolled in the obstetric wards of a tertiary maternity hospital. Urinary incontinence, generic and specific health-related quality of life were assessed using the International Consultation on Incontinence Modular Questionnaire-Urinary Incontinence Short Form (ICIQ-UI SF), the 12-Item Short Form Health Survey version 2 (SF-12v2), Urogenital Distress Inventory short form (UDI-6) and Incontinence Impact Questionnaire short form (IIQ-7), respectively. Multiple logistic regression and multiple linear regression analysis were used to examine risk factors of urinary incontinence during pregnancy and the impact of incontinence on health-related quality of life of pregnant women, respectively.Results: A total of 1243 women were enrolled in the study. The prevalence of urinary incontinence during pregnancy was 52.0%. Most women suffered from mild or moderate incontinence. Five risk factors were identified by multiple logistic regression. Urinary incontinence before pregnancy was the strongest predictor for incontinence during pregnancy (OR = 4.178, 95% CI = 2.690-6.490), followed by history of vaginal birth, coffee consumption, childhood enuresis and history of urinary tract infection. Urinary incontinence had significant impact on health-related quality of life during pregnancy. Only 14.8% of pregnant women sought professional help for urinary symptoms.Conclusions: Urinary incontinence was highly prevalent in pregnant women, with a broad detrimental effect on health-related quality of life. Five factors were confirmed to be associated with increasing the risk of developing urinary incontinence during pregnancy. The help-seeking behavior during pregnancy was discouraging. Targeted interventions are warranted to facilitate the prevention of urinary incontinence and improvement of health-related quality of life in pregnant women. [ABSTRACT FROM AUTHOR]

Objective: Postpartum women experience thyroid dysfunction at twice the prevalence of the general population. Adequate biosynthesis of thyroid hormones depends on three trace elements: iodine, selenium and iron. This study aimed to investigate thyroid dysfunction within a cohort of women at six months postpartum in relation to iodine, selenium and iron status. Design: This cross‐sectional study was part of an observational longitudinal cohort Mother and Infant Nutrition Investigation; data obtained at six months postpartum are reported. Subjects: Mother‐infant pairs (n = 87) were recruited at three months postpartum and followed up at six months postpartum (n = 78). Measurements: Thyroid hormones (free triiodothyronine, free thyroxine, thyroid‐stimulating hormone) and thyroid peroxidase antibodies were measured. Urinary iodine concentration, breast milk iodine concentration, serum thyroglobulin, plasma selenium, serum ferritin and serum soluble transferrin receptors were determined. Nonparametric data were expressed as median (25th, 75th percentile). Results: Thyroid dysfunction was found in 18% of women, and 4% of women had iron deficiency. Median urinary iodine concentration was 85 (43, 134) µg/L, median breast milk iodine concentration was 59 (39, 109) µg/L, and median serum thyroglobulin at 11.4 (8.6, 18.6) µg/L, indicating iodine deficiency. Median plasma selenium concentration was 105.8 (95.6, 115.3) µg/L. Women with marginally lower plasma selenium concentration were 1.12% times more likely to have abnormal TSH concentrations (p =.001). Conclusions: There was a high prevalence of thyroid dysfunction. Plasma selenium concentration was the only significant predictor of the likelihood that women had thyroid dysfunction within this cohort, who were iodine deficient and mostly had adequate iron status. Strategies are required to improve both iodine and selenium status to better support maternal thyroid function. [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate the effects of dietary supplementation of Bacillus licheniformis derived-protease, alone or in combination with valine to low protein diet on growth performance, nutrient digestibility and carcass quality grade in growing-finishing pigs. A total of 180 crossbred ([Landrace × Yorkshire] × Duroc) growing pigs with an initial body weight of 22.56 ± 1.59 kg were used in a 17-week feeding trial. Pigs were randomly allotted into three treatments: CON, basal diet; PROT, low protein diet with 0.05% protease; PROTV, PROT diet with 0.08% valine. During the finishing phase (wk 7–17) and overall period (wk 0–17), gain: feed (G:F) ratio was increased (P < 0.05) in pigs fed the PROTV diet compared with those fed the CON diet. Although the supplementation of B. licheniformis derived-protease in combination with valine to low protein diet increased the G:F ratio of finishing phase and overall, compared with CON, other indexes of growth performance, nutrient digestibility and carcass quality grade were not impacted. Dietary supplementation with a combination of B. licheniformis derived-protease and valine in a low crude protein diet had no deleterious effects on growth performance, nutrient digestibility and carcass quality grade in growing-finishing pigs. [ABSTRACT FROM AUTHOR]

Background Motoric Cognitive Risk (MCR) syndrome, a predementia syndrome characterized by cognitive complaints and slow gait, may have an underlying vascular etiology. Elevated blood levels of homocysteine, a known vascular risk factor, have been linked to physical and cognitive decline in older adults, though the relationship with MCR is unknown. We aimed to identify the association between homocysteine and MCR risk. Methods We examined the association between baseline homocysteine levels and incident MCR using Cox proportional hazard models in 1826 community-dwelling older adults (55% women) from 2 cohorts (Einstein Aging Study [EAS] and Quebec Longitudinal Study on Nutrition and Successful Aging [NuAge]). We calculated hazard ratios (HR) with 95% confidence intervals (CI), for each cohort as well as stratified by sex and vascular disease/risk factors. Results Median follow-up time was 2.2 years in EAS and 3.0 years in NuAge. Individuals with elevated baseline homocysteine levels (>14 µmol/L) had a significantly higher risk of incident MCR compared to those with normal levels in NuAge (HR 1.41, 95% CI: 1.01–1.97, p  = .04), after adjusting for covariates. Our exploratory stratified analyses found that these associations were significant only in men with vascular disease/risk factors. Conclusions Higher blood homocysteine levels are associated with an increased risk of developing MCR in older adults, particularly in men with vascular disease or vascular risk factors. [ABSTRACT FROM AUTHOR]

Objective: Chemoradiotherapy (CRT) is the standard treatment for limited-stage small cell lung cancer (LS-SCLC), which can exert anti-tumor effects by regulating immune cells. Different immune cell subsets are associated with a specific sensitivity to CRT. The purpose of this study was to characterize the proportion or composition of peripheral lymphocytes in patients with LS-SCLC before and after CRT, and evaluate their prognostic value. Methods: A total of 98 patients with LS-SCLC were enrolled. The expression of CD3, CD4, CD8, CD45RA, CD45RO, CD38, CD56, and CD19 on the surface of peripheral blood cells was detected by flow cytometry and retrospectively analyzed. The relationship between the proportion of lymphocyte subsets, progression-free survival (PFS), and overall survival (OS) was evaluated using a log-rank test and Cox regression model. Results: The median PFS was 12.3 months and the median OS was 21.7 months. Compared with the pre-treatment specimens, post-treatment lymphocytes had increased proportions of CD3+, CD3+CD8+, CD8+CD38+ T cells, and NKT cells, and a decreased proportion of CD3+CD4+ T cells, CD4+CD45RA+ T cells, B cells, NK cells, and CD4/CD8 ratio. Univariate and multivariate analyses showed that prophylactic cranial irradiation, high percentages of CD4+CD45RA+, CD8+CD38+ T cells after CRT independently predicted superior PFS. Male patients with a high baseline CD4+CD45RO+ T cell ratio predicted a poor OS. Conclusions: CRT induced changes in the proportion of circulating lymphocyte subsets in LS-SCLC, which is helpful for designing a regimen of immune drugs to be combined with CRT. The prognostic value of the proportion of lymphocytes aids in understanding the role of peripheral immune profiles in LS-SCLC. [ABSTRACT FROM AUTHOR]

Dispersity (Đ) of polymers has a great effect on the properties of polymeric materials, and therefore how to control Đ is very important but still a huge challenge in polymer synthesis, especially for reversible-deactivation radical polymerization (RDRP) strategy. Herein, we successfully developed a novel strategy to adjust Đ of polymers by visible light-controlled reversible complexation mediated living radical polymerization (RCMP) and combination of single-electron transfer-degenerative chain transfer living radical polymerization (SET-DTLRP) at room temperature. In RCMP system, 2-iodo-2-methylpropionitrile (CP-I) and ethyl 2-iodo-2-phenylacetate (EIPA) were used as alkyl iodide initiators, by using methyl methacrylate (MMA) as the model monomer and n-butylacrylate (BA) as the end-capping reagent to regulate Đ of polymers. Subsequently, we successfully prepared the block copolymer PMMA-b-PBA with adjustable Đ by reactivating the polymer end-chains via SET-DTLRP in the presence of copper wire, fully demonstrating that it is a promising strategy that can keep the "living" feature of polymers while regulating their molar mass dispersities easily. [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical characteristics, treatment and prognosis of fetal intracranial hemorrhage in pregnancy and to improve the level of diagnosis and treatment. Methods: We retrospectively analyzed the clinical data of eight cases of fetal intracranial hemorrhage in our hospital from 2014 to 2017, including the clinical manifestations, etiology, imaging features, treatment and prognosis. Results: All the cases were diagnosed by prenatal color ultrasound or magnetic resonance imaging (MRI); one of the cases had decreased fetal movements and abnormal fetal heart rate monitoring, and the remaining seven cases had no special clinical symptoms. No clear cause was found in all the cases. Two patients with grade I fetal intracranial hemorrhage and 1 patient with grade II had a cesarean delivery, and no neurological sequelae were found in these neonates after 6months of follow-up. There was one patient with grade III and four patients with grade IV fetal intracranial hemorrhage; one of the patients with grade IV was stillborn at the time of the discovery, and cesarean section was selected due to scarring of the uterus; intraamniotic injection of ethacridine lactate was selected to induce labor in three cases, and vaginal delivery was selected; one of the patients with grade IV chose vaginal delivery, and the neonatal cranial brain magnetic resonance imaging after delivery showed no increase in intracranial lesions but showed incomplete development of the remaining nervous system. Conclusion: Fetal intracranial hemorrhage can be diagnosed by prenatal color ultrasound and MRI, yet it is often impossible to determine the cause. The prognosis of fetal intracranial hemorrhage is related to grade, and the prognosis of cerebral hemorrhage in patients with grades III-IV is poor. [ABSTRACT FROM AUTHOR]

Aim: To investigate the safety and feasibility of taking low‐concentration carbohydrate solution at 2 h before induction of anesthesia for gestational diabetes mellitus (GDM) patients. Methods: GDM patients undergoing cesarean section were randomly assigned to experimental group (n = 43) and control group (n = 42). Two hours before induction of anesthesia, participants in experimental group orally received 300 mL low‐concentration carbohydrate solution, while those in control group received equivalent warm water. Blood glucose and serum insulin were measured at 2 h before induction of anesthesia, right before induction of anesthesia, and the morning of postoperative day 1. Neonatal blood glucose level was monitored at birth. Maternal gastrointestinal function and well‐being were assessed perioperatively. Results: The levels of blood glucose and serum insulin right before induction of anesthesia in the experimental group were significantly higher than those in the control group. There were four cases with hypoglycemia in the experimental group and 19 cases in the control group right before induction of anesthesia (9.3% vs 45.2%, p < 0.001). The incidence of neonatal hypoglycemia was 2.3% in the experimental group and 7.1% in the control group with no significance. Hunger score of the participants between the two groups right before induction of anesthesia was significantly different. No aspiration, nausea, and vomiting occurred in both groups before, during, and after surgery. No significant difference was found in the time to the first flatus and abdominal distension between the two groups. Conclusion: Taking low‐concentration carbohydrate solution is safe and feasible for patients with GDM undergoing elective cesarean section. [ABSTRACT FROM AUTHOR]

• EA can reverse and raise the pain threshold in hyperalgesic priming model rats. • EA regulates the expression of p-p38, IL-33, and ST2 in astrocytes in SDHs. • EA prevents acute to chronic pain development by inhibiting IL-33/ST2 signaling. • This will help clinicians understand the mechanisms of EA analgesia. Chronic pain is a complex health issue. Compared to acute pain, which has a protective value, chronic pain is defined as persistent pain after tissue injury. Few clinical advances have been made to prevent the transition from acute to chronic pain. Electroacupuncture (EA), the most common form of acupuncture, is widely used in clinical practice to relieve pain. The hyperalgesic priming model, established via a carrageenan injection followed by a prostaglandin E 2 injection, was used to investigate the development or establishment of chronic pain. We observed the hyperalgesic effect of EA on rats and investigated the expression p38 mitogen-activated protein kinase, interleukin-33 (IL-33), and its receptor ST2 in astrocytes in the L4–L6 spinal cord dorsal horns (SDHs) after EA. The IL-33/ST2 signaling pathway in SDH is associated with the development of chronic pain. EA can reverse the pain threshold in hyperalgesic priming model rats and regulates the expression of phosphorylated p38, IL-33, and ST2 in astrocytes in the L4–L6 SDHs. We discovered that EA raises the pain threshold. This suggests that EA can prevent the development or establishment of chronic pain by inhibiting IL-33/ST2 signaling in the lower central nervous system. EA can alleviate the development or establishment of chronic pain by modulating IL-33/ST2 signaling in SDHs. Our findings will help clinicians understand the mechanisms of EA analgesia. [ABSTRACT FROM AUTHOR]

China's large-scale high-speed rail (HSR) construction has raised heated debates worldwide. Differing from previous research on metropolitan areas, this study examines on the economic impacts on periphery cities connected by HSR lines. Using a unique panel dataset of 237 Chinese non-core cities at the prefecture level spanning from 2002 to 2016, this study adopts a spatial difference-in-differences model to investigate the impact of HSR on service industry output. The estimation results suggest that the operation of HSR has a significant negative impact on service industry output of non-core cities, confirming the existence of a siphoning effect of HSR. Such negative impact varies with the profile of the non-core cities. HSR-connected non-core cities in the relatively well-developed coastal region, medium-sized and located within half-an-hour's travel time to mega cities tended to be associated with the most pronounced reductions. These findings provide important implications to the Chinese government and could be cogent for those planning or constructing HSR lines in other countries, given the numerous ongoing HSR initiatives around the globe. • This study adopts a spatial DID model to investigate the economic impact of HSR in connected non-core cities. • Non-core HSR cities exhibit lower service industry output due to the siphoning effect induced by HSR. • Non-core cities located in the better-developed coastal regions experience more service industry losses compared to the interiors. • The medium-sized non-core cities suffer more losses than both the large-sized and small-sized cities. • The non-core cities close to the core cities experience more reductions compared to the faraway. [ABSTRACT FROM AUTHOR]

Intravenous ibuprofen, a nonsteroidal anti-inflammatory drug, is widely used as an antipyretic and analgesic in adults and children. This study was designed to evaluate ethnic differences by comparing the pharmacokinetics of intravenous ibuprofen in Caucasian and Chinese populations using physiologically based pharmacokinetics (PBPK) modeling and simulation. A PBPK model for intravenous ibuprofen was developed in adults and children utilizing the Simcyp Simulator. The model was tested and verified against published literature and unpublished data obtained from the Caucasian adult population, Caucasian pediatric population and Chinese adult population. The developed PBPK model could adequately pilot the pharmacokinetics of intravenous ibuprofen, and the major observed values were within the 90% prediction interval in both adults and children. Both folding errors of the maximum peak concentration (C max) and area under the concentration–time curve (AUC) were 1.5-fold less in the Caucasian and Chinese populations. In addition, no significant differences in weight-normalized C max and AUC were observed between the Caucasian and Chinese adult populations. Moreover, there were no obvious pharmacokinetic differences between the Caucasian and Chinese pediatric populations with intravenous infusion (10 minutes) of 10 mg/kg by age group. This study indicates that the pharmacokinetic profile and the parameters of intravenous ibuprofen are analogous in Caucasian and Chinese populations, either adults or children. In addition, this study provides effective evidence that the dosing scheme of intravenous ibuprofen in Chinese children can remain the same as the regimen that the original company (Caldolor®) provided. [Display omitted] [ABSTRACT FROM AUTHOR]

Background: CPGs are not uniformly successful in improving care and several instances of implementation failure have been reported. Performing a comprehensive assessment of the barriers and enablers is key to developing an informed implementation strategy. Our objective was to investigate determinants of guideline implementation and explore associations of self-reported adherence to guidelines with characteristics of participants in China.Methods: This is a cross-sectional survey, using multi-stage stratified typical sampling based on China's economic regional divisions (the East, the Middle, the West and the Northeast). 2-5 provinces were selected from each region. 2-3 cities were selected in each province, and secondary and tertiary hospitals from each city were included. We developed a questionnaire underpinned by recommended methods for the design and conduct of self-administered surveys and based on conceptual framework of guideline use, in-depth related literature analysis, guideline development manuals, related behavior change theory. Finally, multivariate analyses were performed using logistic regression to produce adjusted odds ratios (OR) and 95% confidence intervals (95% CI).Results: The questionnaire consisted of four sections: knowledge of methodology for developing guidelines; barriers to accessing guideline; barriers to guideline implementation; and methods for improving guideline implementation. There were 1732 participants (87.3% response rate) from 51 hospitals. Of these, 77.2% reported to have used guidelines frequently or very frequently. The key barriers to guideline use were lack of education or training (46.2%), and overly simplistic wording or overly broad scope of recommendations (43.8%). Level of adherence to guidelines was associated with geographical regions (the northeast P < 0.001; the west P = 0.02; the middle P < 0.001 compared with the east), hospital grades (P = 0.028), length of practitioners' practice (P = 0.006), education background (Ph.D., P = 0.027; Master, P = 0.002), evidence-based medicine skills acquired in work unit (P = 0.012), and medical specialty of practitioner (General Practice, P = 0.006; Surgery, P = 0.043).Conclusion: Despite general acknowledgement of the importance of guidelines, the use of guidelines was not as frequent as might have been expected. To optimize the likelihood of adherence to guidelines, guideline implementation should follow an actively developed dissemination plan incorporating features associated with adherence in our study. [ABSTRACT FROM AUTHOR]

Objective: The association between a (GT)n dinucleotide length polymorphism in the promoter region of heme oxygenase 1 (HMOX1) and the risk of neonatal hyperbilirubinemia remains controversial. This meta-analysis was, therefore, performed with aims to examine the correlation between the HMOX1 (GT)n repeat length polymorphism and neonatal hyperbilirubinemia susceptibility.Materials and methods: We searched the databases including PubMed, Embase, Cochrane Library, China national knowledge infrastructure (CNKI), and Wanfang Data, with all reviewed studies published before 28 June 2018. After the evaluation of quality, we used RevMan to perform the meta-analyses. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to assess the effect of HMOX1 gene promoter polymorphisms on the risk of neonatal hyperbilirubinemia.Results: Seven studies, involving 584 patients with neonatal hyperbilirubinemia and 1655 controls, were included. A statistically significant association was found between the HMOX1 (GT)n repeat length polymorphism and risk of neonatal hyperbilirubinemia under the allele (allele S vs. allele L: OR = 1.81, 95% CI = 1.22-2.67, p = .003), recessive (genotype SS vs. genotypes LS + LL: OR = 1.38, 95% CI = 1.02-1.86, p = .04), dominant (genotypes SS + LS vs. LL: OR = 1.37, 95% CI = 1.01-1.76, p = .01), and homozygous genetic models (genotype SS vs. genotype LL: OR = 1.47, 95% CI = 1.02-2.11, p = .003), but not under the heterozygous genetic model. Interestingly, subgroup analysis revealed that the cutoffs of the S allele < 25 showed significant associations in any of the five genetic models (allele S vs. allele L: OR = 2.26, 95% CI = 1.68-3.05, p < .00001; genotype SS vs. genotypes LS + LL: OR = 2.56, 95% CI = 1.41-4.65, p = .002; genotypes SS + LS vs. genotype LL: OR = 1.82, 95% CI = 1.28-2.59, p = .0009; genotype SS vs. genotype LL: OR = 3.09, 95% CI = 1.50-6.36, p = .002; genotype LS vs. genotype LL: OR = 1.64, 95% CI = 1.11-2.42, p = .01); however, this association was not observed in the cutoffs of the S allele ≥25.Conclusion: The results of this study indicate that there is a significant association between the HMOX1 (GT)n repeat length polymorphism and susceptibility to neonatal hyperbilirubinemia. Newborns carrying shorter (GT)n repeats in the HMOX1 gene promoter may have a higher risk of neonatal hyperbilirubinemia. [ABSTRACT FROM AUTHOR]

Cyclical fluctuations of the ovarian hormones estrogen (E2) and progesterone (PROG) have multiple effects on reproduction and development. However, little is known about the roles of E2 and PROG in women's social behaviors. Here, based on evolutionary theory suggesting social sensitivity and inhibition ability are conductive to maintaining social relationships, we provide evidence for the association between menstrual phases and social orientation. In Study 1, 78 women provided saliva samples and reported their intensity of behavioral activation/inhibition system (BAS/BIS) and interpersonal sensitivity at either of two phases of the menstrual cycle: late follicular phase (FP), and mid-luteal phase (LP). A significant between-subject association emerged, revealing that women with higher PROG levels reported higher levels of social feedback sensitivity, and women with relatively high PROG levels showed a positive association between their E2 levels and inhibitory response. In Study 2, 30 women reported their interpersonal anxiety and finished the social value orientation (SVO) measures at both late FP and mid-LP. A significant within-person effect emerged: women in the mid-LP, which is characterized by higher PROG levels, reported higher levels of interpersonal anxiety and SVO. In sum, these findings revealed that women's social orientation could fluctuate naturally with ovarian hormones across the menstrual cycle. [ABSTRACT FROM AUTHOR]

Long chain unsaturated fatty acids (LCUFAs) are emerging as critical contributors to inflammation and its resolution. Sensitive and accurate measurement of LCUFAs in biological samples is thus of great value in disease diagnosis and prognosis. In this work, a fluorous-derivatization approach for UPLC-MS/MS quantification of LCUFAs was developed by employing a pair of fluorous reagents, namely 3-(perfluorooctyl)-propylamine (PFPA) and 2-(perfluorooctyl)-ethylamine (PFEA). With this method, the LCUFAs in biological samples were perfluoroalkylated with PFPA and specifically retained on a fluorous-phase LC column, which largely reduced matrix interferences-induced quantitation deviation. Moreover, PFEA-labeled LCUFAs standards were introduced as one-to-one internal standards to farthest ensure unbiased results. Application of the proposed method enabled a reliable determination of eight typical LCUFAs with high sensitivity (LLOQ ranged from 30 amol to 6.25 fmol) and low matrix interferences (almost less than 10%). Such a high sensitivity could facilitate the determination of small-volume and low-concentration bio-samples. Further metabolic characterization of these targeted LCUFAs was monitored in OVA-induce asthma mice, requiring only 5 μL serum sample. Our results showed that asthmatic attack led to significant disturbances not only in the concentrations but also in the ratio among these LCUFAs. In view of the favorable advantages in sensitivity and accuracy, the present fluorous-paired derivatization approach will be expected to serve as a new avenue for dissecting the physiological and clinical implications of LCUFAs, thereby shedding light on the management of diseases related to their disturbances. Image 1 • A pair of fluorous reagents was employed for profiling of LCUFAs. • Matrix effects were largely reduced by the specific fluorophilicity between fluorinated-LCUFAs. • PFEA-labeled LCUFAs standards were used as one-to-one ISs to farthest ensure unbiased results. • An extreme low detection limit was achieved at an atto-molar level. [ABSTRACT FROM AUTHOR]

Aims Vascular calcification is highly prevalent in atherosclerosis, diabetes, and chronic kidney disease. It is associated with increased morbidity and mortality in patients with cardiovascular disease. Matrix metalloproteinase 3 (MMP-3), also known as stromelysin-1, is part of the large matrix metalloproteinase family. It can degrade extracellular matrix components of the arterial wall including elastin, which plays a central role in medial calcification. In this study, we sought to determine the role of MMP-3 in medial calcification. Methods and results We found that MMP-3 was increased in rodent models of medial calcification as well as in vascular smooth muscle cells (SMCs) cultured in a phosphate calcification medium. It was also highly expressed in calcified tibial arteries in patients with peripheral arterial disease (PAD). Knockdown and inhibition of MMP-3 suppressed phosphate-induced SMC osteogenic transformation and calcification, whereas the addition of a recombinant MMP-3 protein facilitated SMC calcification. In an ex vivo organ culture model and a rodent model of medial calcification induced by vitamin D3, we found that MMP-3 deficiency significantly suppressed medial calcification in the aorta. We further found that medial calcification and osteogenic transformation were significantly reduced in SMC-specific MMP-3-deficient mice, suggesting that MMP-3 in SMCs is an important factor in this process. Conclusion These findings suggest that MMP-3 expression in vascular SMCs is an important regulator of medial calcification and that targeting MMP-3 could provide a therapeutic strategy to reduce it and address its consequences in patients with PAD. [ABSTRACT FROM AUTHOR]

Spillover effect theory posits that work stressors can have spillover effects into workers' home lives. Although job insecurity spillover into the home domain has been explored extensively, potential spillback effects into the work domain have not. We posit that daily job insecurity represents a negative subjective perception that can spillover into the home domain and lead to insomnia, which will damage the recovery of self‐regulatory resources and make employees unable to regulate their own behavior, ultimately resulting in next‐day counterproductive work behavior. We hypothesized that self‐compassion, as an individual trait, weakens the spillover effect of job insecurity and moderates the indirect effect of job insecurity on next‐day counterproductive work behavior via insomnia. Our analyses of data collected from 132 full‐time employees across 10 consecutive working days showed that insomnia mediates the relationship between daily job insecurity and next‐day counterproductive work behavior, and further showed that this relationship was moderated by self‐compassion. Overall, our research captures the cascading effects of daily job insecurity and contributes to a more complete understanding of the spillover effect of job insecurity. [ABSTRACT FROM AUTHOR]

The selective sensing of neurochemicals is essential for understanding the chemical basis of brain function and pathology. Interfacing the excellent recognition features of aptamers with in vivo compatible carbon fiber microelectrode (CFE)‐based electroanalytical systems offers a plausible means to achieve this end. However, this is challenging in terms of coupling chemistry, stability, and versatility. Here, we present a new interfacial functionalization strategy based on the assembly of aptamer cholesterol amphiphiles (aptCAs) on the alkyl chain‐functionalized CFE. The noncovalent cholesterol‐alkyl chain interactions effectively immobilize aptamers onto the CFE surface, allowing the generation of a highly selective system for probing neurochemical dynamics in living systems and opening up a vast array of new opportunities for designing in vivo sensors for exploring brain chemistry. [ABSTRACT FROM AUTHOR]

The selective sensing of neurochemicals is essential for understanding the chemical basis of brain function and pathology. Interfacing the excellent recognition features of aptamers with in vivo compatible carbon fiber microelectrode (CFE)‐based electroanalytical systems offers a plausible means to achieve this end. However, this is challenging in terms of coupling chemistry, stability, and versatility. Here, we present a new interfacial functionalization strategy based on the assembly of aptamer cholesterol amphiphiles (aptCAs) on the alkyl chain‐functionalized CFE. The noncovalent cholesterol‐alkyl chain interactions effectively immobilize aptamers onto the CFE surface, allowing the generation of a highly selective system for probing neurochemical dynamics in living systems and opening up a vast array of new opportunities for designing in vivo sensors for exploring brain chemistry. [ABSTRACT FROM AUTHOR]

The current study examined a role-related difference in the use of playback (one form of repetition) in medical discourse. We adopted a language and social psychology approach and invoked communication accommodation theory (CAT) to explore this discourse. Thirty doctor/older adult dyads were recorded and transcribed verbatim. Findings suggested that the deployment of repetition is a strategy used by the addresser to either complement or converge to the other interlocutor, in order to ensure that the medical instrumental task is efficiently accomplished and interpersonal rapport is established. Our results show that discourse management is the dominant strategy used by speakers when playing back the other's utterances. Our analysis also demonstrates that CAT strategies work interdependently. We conclude that CAT is a valuable framework to elucidate the dynamics of, and the social psychological processes underlying, the practice of repetition in medical interviews. [ABSTRACT FROM AUTHOR]

Objective: This study aimed to identify the disease‐causing mutation of congenital cataract disease in a large northeastern Chinese family. Materials and Methods: The subjects' peripheral blood was collected, their genomic DNA was extracted, mutation screening of candidate genes was performed using polymerase chain reaction, and the amplified products were sequenced. Recombinant C‐terminal enhanced green fluorescent protein‐tagged wild‐type or mutant CRYGD was expressed in HEK293T cells, and the expression pattern was observed under a fluorescence microscope. The CRYGD protein mutation was analyzed via bioinformatics analysis. Results: c.475delG, a novel frameshift mutation in CRYGD, was identified in the affected family members. This mutation causes premature termination of the polypeptide, resulting in truncated p.(Ala159ProfsTer9). According to the bioinformatics analysis results, compared with wild‐type CRYGD, p.(Ala159ProfsTer9) exhibits significantly decreased hydrophilicity. Fluorescence microscopy revealed that p.(Ala159ProfsTer9) aggregates in the cell in the form of granular deposits. Conclusion: In this study, the novel frameshift mutation c.475delG, p.(Ala159ProfsTer9) in CRYGD was identified to cause congenital cataracts in a large Chinese family; increased hydrophobicity of p.(Ala159ProfsTer9) protein may be the underlying mechanism. [ABSTRACT FROM AUTHOR]

The behavioral activation system (BAS) and the behavioral inhibition system (BIS) have been proposed to relate to stable traits that predict inter-individual differences in motivation. Prior reports point dopamine (DA) pathways, mainly including ventral tegmental area (VTA) and substantia nigra (SN), implicate in subserving reward-related functions associated with BAS and inhibitory functions related with BIS. However, as an important factor that affects DA releasing, it remains an open question whether the ovarian hormones may also be related to BIS/BAS. Here, to investigate effects of the estradiol (E2) and progesterone (PROG) on BIS/BAS and related DA pathways, we employed a BIS/BAS scale and the resting-state functional magnetic resonance imaging (fMRI) during the late follicular phase (FP) and the mid-luteal phase (LP). On the behavioral level, when women had high PROG levels, their E2 levels were found positively correlated with BIS scores, but those women whose PROG levels were low, their E2 levels were negative correlation with BIS scores. On the neural level, we demonstrated BAS was related with the VTA pathway, included brain reward regions of nucleus accumbens (NAc) and orbitofrontal cortex (OFC). Meanwhile, the BIS was correlated with the SN-dorsolateral prefrontal cortex (dlPFC) pathway. ROI-based resting-state functional connectivity (RSFC) analyses further revealed that, RSFC between the SN and dlPFC was modulated by ovarian hormones. With higher PROG levels, increased E2 levels among women were accompanied by stronger RSFC of the SN-dlPFC, but when PROG levels were low, E2 levels were negatively correlated with the SN-dlPFC RSFC. These findings revealed a combined enhancement effect of E2 and PROG on BIS, and the SN-dlPFC pathway was mainly involved in this process. [ABSTRACT FROM AUTHOR]

A correction to this paper has been published: https://doi.org/10.1007/s00262-021-02929-0 [ABSTRACT FROM AUTHOR]

Single-ion monitoring is a key requirement for many energetic heavy-ion experiments, e.g., the laboratory simulation of the single event effect of semiconductor devices under heavy ion bombardments. We have developed a two-dimensional position-sensitive and timing monitor of individual ions. It is composed of a thin aluminum foil, a pair of microchannel plate detectors, and electrostatic and magnetic fields. When energetic heavy ions pass through the aluminum foil, secondary electrons generated on each side of the foil are guided by the fields to the corresponding detector. Both the hitting position and the arrival time of the secondary electrons on corresponding detectors are measured in coincidence. A test with an 241Am α source shows that the present monitor is capable of discriminating true events from heavy background radiations. A position resolution of 1.0 mm and a recording time resolution of 50 ns have been realized in the test. [ABSTRACT FROM AUTHOR]

The crystal of a new tetrachlorocobalt(II) anion salt, [BzPy]2[CoCl4] (1) ([BzPy]+ = benzyl pyridinium), was grown by the slow evaporation solution growth method at room temperature. The salt 1 crystallizes in the monoclinic space group P2(1)/c and consists of two [BzPy]+ cations and one [CoCl4]2− anion in which the Co(II) ion is coordinated by four Cl atoms in a close to tetrahedral geometry. The crystal packing is stabilized by electrostatic interactions and C − H···Cl hydrogen bonds between [BzPy]+ cations and [CoCl4]2− anions. Powder XRD diffraction study confirms the crystalline nature and purity of the title compound. The Fourier transform infrared (FT-IR) spectrum was recorded and analyzed with the aid of DFT calculations in order to make a suitable assignment of the observed bands. The thermal stability of the hybrid crystal was analyzed by TG-DTA techniques revealing that the title crystal was stable up to 195 °C. The title salt exhibits violet and cyan fluorescence emissions upon excitation at 345 nm and weak antiferromagnetic interactions in the temperature range 2-300 K. The nonlinear optical (NLO) effect of this compound was predicted using DFT, suggesting that the title crystal is a good material for NLO applications. [ABSTRACT FROM AUTHOR]

This research examines the performance of the previously proposed cutoff values of alternative fit indices (i.e., change in comparative fit index [ ΔCFI ], change in Tucker–Lewis index [ ΔTLI ], and change in root mean squared error of approximation [ Δ RMSEA ]) to evaluate measurement invariance for exploratory structural equation modeling (ESEM) models with simulated data. It is important to revisit these cutoff values because they were used widely in validity studies utilizing ESEM models to evaluate measurement invariance for ordinal indicators, but in fact, these cutoff values were developed under confirmatory factor analysis models with continuous indicators. Results of this study show that different cutoff values of ΔCFI , ΔTLI , and Δ RMSEA should be used for ESEM models with ordinal indicators. Evaluation of partial invariance for ESEM models is also discussed. [ABSTRACT FROM AUTHOR]

Background. Both experimental and clinical studies have shown that electroacupuncture (EA) administration ameliorates chronic inflammatory pain (CIP). However, the multifaceted mechanism underlying the effects of EA on CIP is poorly understood. In this study, the mRNA transcriptome was used to study various therapeutic targets of EA. Methods. Using RNA-sequencing, protein-coding mRNA expression profiles of the L4-L5 dorsal root ganglion (DRG) were examined in the control (CN), complete Freund's adjuvant- (CFA-) induced CIP, and EA-treated CIP groups. A series of bioinformatics analyses was performed; "EA-reversed upregulated genes with CIP" (up-DEGs) and "EA-reversed downregulated genes with CIP" (down-DEGs) were identified. Thereafter, based on up-DEGs and down-DEGs, biological functions and signaling pathways were enriched using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses. Results. In total, 189 DEGs were identified, including 134 up- and 55 down-DEGs, which were enriched in arachidonic acid metabolism (rno00590), glutamatergic synapse (rno04724), serotonergic synapse (rno04726), FoxO signaling pathway (rno04068), insulin signaling pathway (rno04910), amyotrophic lateral sclerosis (rno05014), cholinergic synapse (rno04725), ECM-receptor interaction (rno04512), and choline metabolism in cancer (rno05231). Conclusion. We identified a few GOs, pathways, and genes that could play key roles in the amelioration of CIP by EA. Hence, this study may provide a theoretical basis for CIP amelioration by EA. [ABSTRACT FROM AUTHOR]

Objective: We carried out a meta-analysis of case-control studies to determine whether epoxide hydrolase 1 (EPHX1) gene polymorphism rs1051740 was related to the risk of ovarian cancer. Methods: Electronic databases were searched for relevant articles published in English or Chinese language. We calculated crude odds ratios (ORs) with their 95% confidence intervals (95% CIs) to assess the relationship of EPHX1 polymorphism rs1051740 with ovarian cancer risk. In addition, subgroup analyses were also conducted based on ethnicity and control source. Between-study heterogeneity was inspected with Q test and I2 statistic. Results: Five eligible studies with a total of 1919 ovarian cancer patients and 1829 controls were ultimately included in the present meta-analysis. Overall results demonstrated that the association between EPHX1 polymorphism rs1051740 and ovarian cancer risk had no statistical significance either in total analysis or in subgroup analyses by ethnicity and source of control. Conclusion:EPHX1 polymorphism rs1051740 may have no independent effect on ovarian cancer susceptibility. [ABSTRACT FROM AUTHOR]

Dissolved gas analysis (DGA) is an effective technique to evaluate the operation status of the power transformer, and to guarantee the safe operation of the power system. Using density functional theory (DFT), we proposed the application of Pd-doped SnS2 (Pd-SnS2) monolayer as a sensing material to detect H2 and C2H2 in transformer oil. The electronic and geometric structures of Pd-doping behavior on SnS2 were also analyzed to give insight into the physicochemical property of the active surface. The adsorption configuration, density of state (DOS), frontier molecular orbital theory as well as recovery property of the adsorption systems were comprehensively studied to estimate the suitability of our proposed material for sensing application. Our calculations showed that Pd-doping exerts some deformations on the SnS2 monolayer and leads to the narrowed bandgap of such surface. Meanwhile, Pd-SnS2 monolayer has stronger adsorption performance upon C2H2 compared to H2. And the desirable interaction with C2H2 makes it possible for application of a resistance-type sensor given the good electrical response and recovery property. Our calculations are meaningful to suggest novel sensing material for application in the field of electrical engineering. [ABSTRACT FROM AUTHOR]

Aims and objectives: To compare the differences in psychosocial behaviour reactions, psychosocial needs, anxiety and depression before and after colostomy surgery and to explore the predictors of psychosocial behaviour reactions. Background: Colorectal cancer is increasing worldwide. Abdominoperineal resection accompanied by permanent colostomy can cause complicated psychosocial reactions and needs. However, colostomy patients' trajectories of psychosocial adjustment at different time points must be discussed. Design: A longitudinal study. Methods: Using a convenience sampling method, 67 patients planning to undergo colostomy surgery were recruited from a tertiary cancer centre in southern China from January 2013–January 2014. Data collection consisted of two phases, conducted 1–2 days before surgery and 1–2 days prior to discharge. The Ostomy Psychosocial Behaviour Reaction Questionnaire, the Ostomy Psychosocial Needs Questionnaire, the Hospital Anxiety and Depression Scale and a self‐designed demographic questionnaire were used to collect data. STROBE guidelines were followed. Results: The participants had a moderate level of psychosocial behaviour reactions, a deep and wide range of psychosocial needs and a high incidence of anxiety and depression before and after surgery. Anxiety before surgery was significantly higher than that after surgery. Psychosocial needs and anxiety were significant predictors of psychosocial behaviour reactions. Conclusions: Patients' anxiety was alleviated after surgery. The combination of ascertaining and meeting various psychosocial needs and managing negative emotions was helpful in alleviating patients' stress responses before and after surgery. Relevance to clinical practice: An increased focus on the relationships among psychosocial behaviour reactions, psychosocial needs, anxiety and depression at different time points in colostomy patients is necessary for future research and practice. [ABSTRACT FROM AUTHOR]

Bone and tooth, fundamental parts of the craniofacial skeleton, are anatomically and developmentally interconnected structures. Notably, pathological processes in these tissues underwent together and progressed in multilevels. Extracellular vesicles (EVs) are cell‐released small organelles and transfer proteins and genetic information into cells and tissues. Although EVs have been identified in bone and tooth, particularly EVs have been identified in the bone formation and resorption, the concrete roles of EVs in bone and tooth development and diseases remain elusive. As such, we review the recent progress of EVs in bone and tooth to highlight the novel findings of EVs in cellular communication, tissue homeostasis, and interventions. This will enhance our comprehension on the skeletal biology and shed new light on the modulation of skeletal disorders and the potential of genetic treatment. [ABSTRACT FROM AUTHOR]

Wastewater treatment is a complex process that involves many uncertainties, leading to fluctuations in effluent quality and costs, and environmental risks. Artificial intelligence (AI) can handle complex nonlinear problems and has become a powerful tool for exploring and managing wastewater treatment systems. This study provides a summary of the current status and trends in AI research as applied to wastewater treatment, based on published papers and patents. Our results indicate that, at present, AI is primarily used to evaluate removal of pollutants (conventional, typical, and emerging contaminants), optimize models and process parameters, and control membrane fouling. Future research will likely continue to focus on removal of phosphorus, organic pollutants, and emerging contaminants. Moreover, analyzing microbial community dynamics and achieving multi-objective optimization are promising directions of research. The knowledge map shows that there may be future technological innovation related to predicting water quality under specific conditions, integrating AI with other information technologies and utilizing image-based AI and other algorithms in wastewater treatment. In addition, we briefly review development of artificial neural networks (ANNs) and explore the evolutionary path of AI in wastewater treatment. Our findings provide valuable insights into potential opportunities and challenges for researchers applying AI to wastewater treatment. [Display omitted] • Utilizing patents and papers of AI in wastewater treatment for data-driven analysis. • Analysis of the current state of research through bibliometrics. • Analyzed the technological evolution of AI in wastewater treatment. • The future trends and technological innovation directions are foreseen. [ABSTRACT FROM AUTHOR]

We report a 36-year-old male patient died of V. vulnificus-induced septicaemia and multiple organ failure syndrome after oyster consumption at a restaurant. We isolated and identified V. vulnificus vv16015 from the patient's blood sample and antibiotic susceptibility tests indicated sensitivity to all 21 antibiotics. Oyster samples were subsequently collected from the restaurant's supplier and three strains of V. vulnificus were isolated. Whole genome sequencing and analysis revealed vv16015 to be distantly related to these strains and confirmed that V. vulnificus contamination was present in the seafood of the restaurant and supplier. Using a Galleria mellonella larvae infection model, the virulence of vv16015 was determined to be higher than that of comparison strains isolated from a surviving patient (vv15018) and an oyster (vv220015). The human and environment distribution of V. vulnificus in Shenzhen is sporadic and heterogeneous, and vv16015 is highly virulent compared to other strains. [ABSTRACT FROM AUTHOR]

Objective: The aim of this study is to investigate potential risk factors associated with peripheral nervous system lesions in primary Sjögren's syndrome (pSS) through a retrospective analysis of clinical manifestations, examination characteristics, and clinical electrophysiological features. Materials and methods: A retrospective case–control study was conducted at Nanfang Hospital, including 108 patients diagnosed with pSS following the criteria revised by the American College of Rheumatology in 2016. The study spanned from January 2015 to October 2020. The patient cohort was divided into two groups, an experimental group (N = 27) consisting of patients with primary Sjögren's syndrome-peripheral nervous system lesions (pSS-PNS), and a control group (N = 81) comprising patients without peripheral neurological impairment, i.e., primary Sjögren's syndrome-non peripheral nervous system lesions (pSS-nPNS). Results: The results showed a significant correlation between immunoglobulin G (IgG), α-Fodrin immunoglobulin G (α-FIgG), platelet counts (PLT), dry mouth and peripheral neuropathy of Sjogren's syndrome (p < 0.01). The research also revealed that α-FIgG (OR 2.03; 95% CI 1.14–3.64), IgG (OR 1.23; 95% CI 1.06–1.42), and PLT (OR 1.01; 95% CI 1.00–1.01) were identified as risk factors for the onset of peripheral neuropathy of Sjogren's syndrome, while dry mouth had a negative correlation (OR 0.08; 95% CI 0.02–0.40). Remarkably, the total risk assessment of the independent variables demonstrated a high AUC (95%CI) of 0.923 (0.861–0.986; p < 0.001), indicating an excellent prediction of pSS-PNS occurrence through the ROC analysis. Additionally, high platelet counts and strong positive anti-SSB antibody titer were found to be risk factors for dual motor and sensory nerve damages among pSS-PNS patients. Conclusion: IgG, α-FIgG, and PLT were identified as independent risk factors for patients with pSS-PNS. The likelihood of peripheral neuropathy appeared to increase in tandem with the elevated levels of above three factors. Interestingly, we found that dry mouth might play a protective role in this context. Our study further noted that both high platelet counts and strong positive anti-SSB antibody titer may be associated with increased risk of both motor and sensory nerve involvement in pSS-PNS patients. These findings have significant implications for both the etiologies and therapeutics of pSS-PNS. [ABSTRACT FROM AUTHOR]

Greenspaces at the city scale, like greenbelts, green-wedges or green-grids, have become well-known instruments for shaping urban economic activity and land use. The economic impacts of such instruments are complex and hard to measure. This article addresses one of the rarely studied problems of measuring the economic impacts of alternative greenspace configurations in fast growing cities. In such cities, there is an uncertain basis for making such greenspace related decisions, for example the assumptions about the cities' total population and economic activity. Decision makers have few tools to measure and predict the potential economic costs and benefits of alternative greenspace configurations. We present a new model that allows tracking over time of both non-divisible land use decisions and a multitude of gradual adaptations made by businesses and consumers. The model is applied to Greater Beijing, one of the fast growing cities that is actively exploring alternative greenspace configurations to control urban expansion. The modelling results suggest that compared with the trend-development scenario of no greenspace intervention, a strict greenbelt would decrease the overall consumer surplus in Beijing by US$3.3 billion, while an adaptive mix of green-wedges and green-grid would increase consumer surplus by US$3.6 billion per year in 2030. The adaptive configuration also reduces car journeys by 11% in Beijing. More generally, modelling results show that a flexible design of strategic greenspaces and careful siting of new development around metro stations within the designated greenspaces could benefit consumers and promote sustainable travel. [ABSTRACT FROM AUTHOR]

This paper presents an average dwell-time method for fault-tolerant control of a class of uncertain switched linear time-delay systems and also designs an algorithm to determine the parameters pertaining to the dwell-time condition. Compared to the literature results, this paper features the following: 1) the proposed method is independent of switching polices if switchings are slow enough in the average sense; 2) the proposed control design guarantees exponential stabilization of this class of time-delay linear systems with actuator faults; and 3) this method treats all actuators in a unified manner without a need to identify which are faulty or healthy actuators. The proposed method is applied to the water pollution control problem, for the first time, to deal with different ratios of stream flow rates to effluent flow rates. [ABSTRACT FROM AUTHOR]

Abstract Renal fibrosis is the common final outcome of nearly all progressive chronic kidney diseases (CKD) that eventually develop into end-stage renal failure, which threatens the lives of patients. Currently, there are no effective drugs for the treatment of renal fibrosis. However, studies have shown that certain plant natural products have a fibrosis-alleviating effect. Thus, we have screened a large number of natural products for their ability to protect against renal fibrosis and found that bisdemethoxycurcumin has a good therapeutic effect in renal fibrosis according to the data obtained in a mouse model of unilateral ureteral obstruction (UUO). The results indicate that bisdemethoxycurcumin can efficiently attenuate renal fibrosis induced by UUO. Additional studies of the bisdemethoxycurcumin mechanism of action in the treatment of renal fibrosis demonstrated that the therapeutic effect of bisdemethoxycurcumin is mediated by the specific induction of fibroblast apoptosis at a concentration of 20 μM. bisdemethoxycurcumin can efficiently protect against renal fibrosis both in vitro and in vivo. This discovery will provide new ideas for renal fibrosis treatment in clinics and a new direction for the development of effective drug therapy of renal fibrosis. [ABSTRACT FROM AUTHOR]

Vulnerability segmentation (VS), an important mechanism for protecting plants from drought, hypothesizes that the distal organs of a plant should be more susceptible to embolism than the basal organs. However, experimental studies testing the VS hypothesis for trees are limited and have reached inconsistent conclusions. Here, we tested the VS hypothesis with three angiosperms and four conifers co-existing in a temperate forest in northeastern China. The results showed that the difference in vulnerability to cavitation between leaves and branches (P 50leaf–branch) was positive for the conifers but negative for the angiosperms, implying that the conifers rather than the angiosperms exhibited VS. The conifers had lower leaf hydraulic safety margins and more embolism-resistant branches than the angiosperms. Although the angiosperms did not display VS, they took a hydraulic compensatory strategy (e.g. great leaf and branch hydraulic conductivities) to maintain the water supply of their leaves. In addition, we found a significant trade-off between the sapwood-specific hydraulic conductivity (K SS) and xylem pressure inducing 50% loss of hydraulic conductivity (P 50branch) across all species. Both K SS and P 50branch increased with the area-based light-saturated photosynthetic rate (A area), suggesting that increased embolism resistance of branches comes at the cost of reduced hydraulic efficiency, which in turn constrains the photosynthesis. A area was negatively correlated with P 50leaf–branch, further indicating that the conifers had strong VS and were associated with a conservative strategy. Conversely, the angiosperms displayed an acquisitive strategy, tending to have higher A area, leaf and branch hydraulic conductivities, but lower embolism resistance. These differentiations in the functional traits between the angiosperms and conifers provide potential mechanisms for their co-existence in this temperate forest community. [ABSTRACT FROM AUTHOR]

Abstract We show that a regular (paratopological) semitopological group G admits a homeomorphic embedding as a subgroup into a product of strongly metrizable (paratopological) semitopological groups if and only if G is strictly ω -balanced with a countable index of regularity. Also, we prove that a topological group G admits a homeomorphic embedding as a subgroup into a product of strongly metrizable groups if and only if G is strictly ω -balanced. Using this fact we obtain a characterization of closed subgroups of a product of strongly metrizable groups. [ABSTRACT FROM AUTHOR]

Abstract Different structures and different Ag+-response behaviors of a pair of pyrene-based S-containing atropisomers, namely s -BSPPy and a -BSPPy , have been investigated. The calculated high rotation barrier indicates that the two atropisomers are non-interconvertible. In polar solvents, s -BSPPy exhibits excimer emission but a -BSPPy does not. Based on the X-ray structures and the DFT calculated intermolecular interactions, the excimer of s -BSPPy is suggested to take a non-parallel edge-to-face λ -shaped geometry. Especially, s -BSPPy exhibits ratiometric excimer-off and monomer-on emission as a unique response to Ag+ in solutions, while no ratiometric Ag+-response was observed for a -BSPPy. Graphical abstract Unlabelled Image Highlights • In solution, s -BSPPy exhibits excimer emission, a -BSPPy does not. • Edge-to-face λ -shaped excimer of s -BSPPy. • s -BSPPy shows excimer-to-monomer emission in response to Ag+, a -BSPPy does not. • s -BSPPy and a -BSPPy atropisomers are non-interconvertible. [ABSTRACT FROM AUTHOR]

The Fe 52 Co 34 Hf 7 B 6 Cu 1 amorphous alloy ribbons were prepared by single-roller-quenching method and then treated by medium-frequency magnetic pulse. The microstructural changes of specimens before and after magnetic pulse treatment were observed by TEM and Mössbauer spectroscopy techniques. The magnetostriction coefficient of specimens before and after the medium-frequency magnetic pulse was measured by homemade magnetostriction coefficient measurement system. The results showed that the specimens were nano-crystallized after treated by medium-frequency magnetic pulse. The crystalline phase was α-FeCo which dispersed in the residual amorphous matrix phase to form the two-phase nanocrystalline alloys. The magnetostriction coefficient of specimens after treated by medium-frequency magnetic pulse was composed of the positive magnetostriction coefficient of amorphous matrix phase and the negative magnetostriction coefficient of nanocrystalline phase. With the increase of the crystallization volume fraction, the magnetostriction coefficient λ decreased. And accompanied with the increase of the applied DC magnetic field intensity H , the magnetostriction coefficient λ firstly increased and then decreased. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • Liposomal drug delivery systems have shown good encapsulation efficiency for curcumin. • Liposomal curcumin ideally overcomes the low solubility issues with natural curcumin. • Liposomal curcumin provides extended release providing improved patient compliance. • The curcumin liposomes showed suppression of important pro-inflammatory markers. • Molecular modelling data reveals the formation of liposomal structure. Abstract Curcumin a component of turmeric, which is derived from Curcuma longa is used as a colouring agent and as a dietary spice for centuries. Extensive studies have been done on the anti-inflammatory activity of curcumin along with its molecular mechanism involving different signalling pathways. However, the physicochemical and biological properties such as poor solubility and rapid metabolism of curcumin have led to low bioavailability and hence limits its application. Current therapies for asthma such as bronchodilators and inhaled corticosteroids (ICS) are aimed at controlling disease symptoms and prevent asthma exacerbation. However, this approach requires lifetime therapy and is associated with a constellation of side effects. This creates a clear unmet medical need and there is an urgent demand for new and more-effective treatments. The present study is aimed to formulate liposomes containing curcumin and evaluate for its anti-inflammatory effects on lipopolysaccharide (LPS)-induced inflammation on BCi-NS1.1 cell line. Curcumin and salbutamol liposomes were formulated using lipid hydration method. The prepared liposomes were characterized in terms of particle size, zeta potential, encapsulation efficiency and in-vitro release profile. The liposomes were tested on BCI-NS1.1 cell line to evaluate its anti-inflammatory properties. The various pro-inflammatory markers studied were Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-1β (IL-1β) and Tumour Necrosis Factor-a (TNF-a). Additionally, molecular mechanics simulations were used to elucidate the positioning, energy minimization, and aqueous dispersion of the liposomal architecture involving lecithin and curcumin. The prepared curcumin formulation showed an average size and zeta potential of 271.3 ± 3.06 nm and −61.0 mV, respectively. The drug encapsulation efficiency of liposomal curcumin is 81.1%. Both curcumin-loaded liposomes formulation (1 μg/mL, 5 μg/mL) resulted in significant (p < 0.05) reduction in the level of pro-inflammatory marker expression such as IL-6, IL-8, IL-1β and TNF-a compared to positive control group. Liposomal curcumin with the dose of 1 μg/mL reduced the inflammatory markers more effectively compared to that of 5 μg/mL. Liposomal curcumin could be a promising intervention for asthma therapy showing their efficacy in suppressing the important pro-inflammatory markers involved in the pathogenesis of asthma. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • An UHPLC-LTQ-Orbitrap oriented 3-nitrophenylhydrazine-based derivatization method was developed and validated. • Short-chain fatty acids (SCFAs) in bronchoalveolar lavage fluid was firstly determined. • Wide linear ranges, high sensitivity and accuracy of this method were demonstrated. • The universality of this method gives a support to deduce the origin of SCFAs in lung. Abstract Recent studies have demonstrated the important role of short-chain fatty acids (SCFAs) in the maintenance of homeostasis of respiratory immunity. However, there is still no report focus on the determination of SCFAs level in bronchoalveolar lavage fluid (BALF), the most common sample used for screening biomarkers of the pulmonary diseases. Herein, an ultra-high-performance liquid chromatography with LTQ-Orbitrap mass spectrometer (UHPLC-LTQ-Orbitrap) oriented 3-nitrophenylhydrazine (3-NPH)-based derivatization method was developed for the quantification of SCFAs in BALF. To achieve accurate quantitation, d4-acetate was used as internal standard to compensate for the matrix effects. Method validation showed a good linearity (R 2 > 0.9992) with wide concentration range, and the intra-day and inter-day precision for determination of eight SCFAs in BALF samples was ≤ 14.79%. The quantitation accuracy, assessed by relative recoveries, ranged from 90% to 110% for target SCFAs at three concentration levels. Matrix effects ranged from 85% to 115%, and the lower limits of quantification of these targeted SCFAs were varied from 3 to 24 nmol/L. The SCFAs-targeted method was then applied to determine the changed levels in BALF samples from OVA-induced asthma mice and normal mice. In addition, the universality of our developed method was also demonstrated by determining the SCFAs concentrations in feces, serum and lung tissue samples from asthma and normal mice. These results indicate that 3-NPH derivatization based UHPLC-LTQ-Orbitrap provides accurate view of global SCFAs alternation in different samples, giving a support to deduce the origin of SCFAs in lung. The present study is of great importance for understanding the role of SCFAs in modulation of host metabolism and immunity. [ABSTRACT FROM AUTHOR]

Dasatinib shows remarkable activity against imatinib-refractory chronic myelogenous leukemia (CML) and Philadelphia chromosome positive acute lymphoblastic leukemia (Ph + ALL). However, severe cardiovascular toxicity limits the clinical applications of dasatinib. Since the underlying mechanism of dasatinib-induced cardiotoxicity is still elusive, we aim to clarify this. Recent studies have shown that necroptosis and apoptosis participate in multiple toxicity development. Here, we first report that dasatinib could directly induce cardiomyocytes death, as analyzed by the Sulforhodamine B (SRB) assay. This type of cardiomyocytes death was mediated by the necrosis pathway rather than apoptosis, as determined by using flow cytometry to characterize the mode of dasatinib-induced cell death. Inhibition of receptor-interacting protein kinase 1 (RIP1)activity and knockdown of receptor-interacting protein kinase 3 (RIP3)expression can block dasatinib-evoked cardiotoxicity, which further confirmed the involvement of necroptosis. We next found that the classic substrates of RIP3, mixed lineage kinase domain-like protein (MLKL) and Ca 2+ -calmodulin-dependent protein kinase II (CaMKII) were not involved in dasatinib-induced cardiomyocytes necroptosis. What’s more, unlike the inflammation-associated necroptosis, dasatinib-triggered necroptosis was dependent on intracellular instead of secreted High-mobility group box 1 (HMGB1) protein. Collectively, our study revealed that dasatinib-induced cardiotoxicity acted via leading cardiomyocytes to HMGB1-mediated necroptosis, indicating a viable strategy for prevention of dasatinib-induced cardiotoxicity. [ABSTRACT FROM AUTHOR]

Although small talk has conventionally been treated as marginal and unimportant compared with core business talk, its value in understanding institutional norms and power relations has been recognized in many workplace contexts. However, in-depth analysis of the dynamics of small talk is still under-researched in clinical contexts. This paper explores where and how small talk is positioned, initiated, and closed between participants in two types of medical practices that co-exist in China: Traditional Chinese Medicine and Western Medicine. Analysis of 69 consultations suggests that small talk permeates into the boundaries of talk. The findings also demonstrate a marked clinical difference in relation to the distribution and discourse functions of small talk. [ABSTRACT FROM AUTHOR]

Periodontitis is a bacteria-induced disease that often leads to alveolar bone damage. We sought to determine the role and mechanism of switched memory B cells in alveolar bone destruction during periodontitis. Sensitized B cells were sorted and cultured, then their expression of receptor activator for nuclear factor-κB ligand (RANKL), interleukin-6 (IL-6), and interleukin-12 (IL-12) was detected. Using these cells, we prepared adoptive transfer models in which we induced periodontitis. We found that switched memory B cells produced more RANKL in terms of both protein and mRNA levels than other subpopulations. Switched memory B cells expressed more IL-6 and IL-12 mRNA than other subpopulations, but differences in respective protein levels were not significant. Moreover, we found that switched memory B cell transfer resulted in increased alveolar bone loss and periodontal osteoclastogenesis. Moreover, switched memory B cell transfer increased the proportion of Th1 and Th17 cells as well as the expression of RANKL, osteoprotegerin (OPG), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IL-1β, IL-6, IL-17A in gingiva, and cervical lymph nodes (CLNs). The outcomes of the present study indicate that switched memory B cells regulate alveolar bone homeostasis via enhancing cytokine expression and increasing proliferation of Th1 and Th17 cells. [ABSTRACT FROM AUTHOR]