Search

Your search keyword '"Kobayashi, Keita"' showing total 428 results
Start Over Author "Kobayashi, Keita"
428 results on '"Kobayashi, Keita"'

54. Precise and Facile Endotracheal Lung-tumor-implantation Mouse Model Visualized by GFP Expression.

Author

YUSUKE AOKI, KOBAYASHI, KEITA J., VARSHNEY, NUNDINI, KUBOTA, YUTARO, MASAKI, NORIYUKI, OBARA, KOYA, WANG, JUSTIN D., YASUNORI TOME, BOUVET, MICHAEL, KOTARO NISHIDA, and HOFFMAN, ROBERT M.

Subjects
TREATMENT of lung tumors, GREEN fluorescent protein, OSTEOSARCOMA, X-ray imaging, ANESTHESIA
Abstract

Background/Aim: In osteosarcoma, lung metastasis is a major cause of cancer-related death, as the 5-year survival rate for patients with metastases is approximately only 20-30%. To develop improved therapeutic strategies against lungmetastatic osteosarcoma, an experimental lung-tumorimplantation mouse model is needed for basic research. In the present study, we developed a precise and facile endotracheal lung-tumor-implantation technique. Materials and Methods: For establishment of the lung-tumor-implantation mouse model of metastatic osteosarcoma, 5 mice were used. A 15-mm longitudinal incision was made in the center of the neck to expose the salivary glands. The salivary glands were then split, exposing the trachea covered by the sternohyoid muscles. The trachea was then clearly exposed by cutting the sternohyoid muscles longitudinally. A 22 G gavage needle was tilted slightly toward the left side of the mouse and inserted from the oral cavity into the bronchus, with confirmation of the position of the tip of the gavage needle visualized through the tracheal wall, followed by injection of 0.5% crystal violet to first confirm the accuracy of endotracheal injection in the lung. A 143B-GFP cell suspension (2.0×106 cells/50 µl PBS) was then injected endotracheally in other mice. Results: The procedure, including anesthesia and suturing, took approximately 10 minutes. The left lobe of the lung, in which crystal violet was injected endotracheally, was stained in 3 out of 3 mice (100%). 143B-GFP-osteosarcoma tumors were detected with GFP fluorescence in the left lobe of the lung in 3 out of 4 mice (75%), 5 weeks after endotracheal injection. One mouse died 4 weeks after 143B-GFP-cell implantation. Conclusion: This novel technique of establishing tumors in the lung via endotracheal injection of cancer cells is precise and facile and can be used widely, since neither a surgical microscope nor Xray imaging are needed. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

61. Bladder cancer prospective cohort study on high‐risk non‐muscle invasive bladder cancer after photodynamic diagnosis‐assisted transurethral resection of the bladder tumor (BRIGHT study)

Author

Kobayashi, Keita, primary, Matsuyama, Hideyasu, additional, Kawai, Taketo, additional, Ikeda, Atsushi, additional, Miyake, Makito, additional, Nishimoto, Koshiro, additional, Matsushita, Yuto, additional, Komura, Kazumasa, additional, Abe, Takashige, additional, Kume, Haruki, additional, Nishiyama, Hiroyuki, additional, Fujimoto, Kiyohide, additional, Oyama, Masafumi, additional, Miyake, Hideaki, additional, Inoue, Keiji, additional, Mitsui, Takahiko, additional, Kawakita, Mutsushi, additional, Ohyama, Chikara, additional, Mizokami, Atsushi, additional, and Kuroiwa, Hajime, additional

Published
2022
Full Text
View/download PDF

64. Formation Mechanism and Toxicological Significance of Biogenic Mercury Selenide Nanoparticles in Human Hepatoma HepG2 Cells

Author

Tanaka, Yu-ki, primary, Usuzawa, Hana, additional, Yoshida, Miyu, additional, Kumagai, Kazuhiro, additional, Kobayashi, Keita, additional, Matsuyama, Satoshi, additional, Inoue, Takato, additional, Matsunaga, Akihiro, additional, Shimura, Mari, additional, Ruiz Encinar, Jorge, additional, Costa-Fernández, José M., additional, Fukumoto, Yasunori, additional, Suzuki, Noriyuki, additional, and Ogra, Yasumitsu, additional

Published
2021
Full Text
View/download PDF

77. Serotonin neurons in the median raphe nucleus bidirectionally regulate somatic signs of nicotine withdrawal in mice

Author

Nishitani, Naoya, 1000080597659, Ohmura, Yu, Kobayashi, Keita, Murashita, Taichi, Yoshida, Takayuki, 1000040182729, Yoshioka, Mitsuhiro, Nishitani, Naoya, 1000080597659, Ohmura, Yu, Kobayashi, Keita, Murashita, Taichi, Yoshida, Takayuki, 1000040182729, and Yoshioka, Mitsuhiro

Abstract

In chronic smokers, nicotine withdrawal symptoms during tobacco cessation can lead to smoking relapse. In rodent models, chronic exposure to nicotine elicited physical dependence, whereas acute antagonism of nicotinic acetylcholine receptors (nAChRs) immediately precipitated withdrawal symptoms. Although the central serotonergic system plays an important role in nicotine withdrawal, the exact serotonergic raphe nuclei regulating these symptoms remain unknown. We used transgenic mice expressing archaerhodopsinTP009 or channelrhodopsin-2[C128S] exclusively in the central serotonergic neurons to selectively manipulate serotonergic neurons in each raphe nucleus. Nicotine withdrawal symptoms were precipitated by an acute injection of mecamylamine, a nonspecific nAChR antagonist, following chronic nicotine consumption. Somatic signs were used as measures of nicotine withdrawal symptoms. Acute mecamylamine administration significantly increased ptosis occurrence in nicotine drinking mice compared with that in control-drinking mice. Optogenetic inhibition of the serotonergic neurons in the median raphe nucleus (MRN), but not of those in the dorsal raphe nucleus (DRN), mimicked the symptoms observed during mecamylamine-precipitated nicotine withdrawal even in nicotine-na & iuml;ve mice following the administration of acute mecamylamine injection. Optogenetic activation of the serotonergic neurons in the MRN nearly abolished the occurrence of ptosis in nicotine drinking mice. The serotonergic neurons in the MRN, but not those in the DRN, are necessary for the occurrence of somatic signs, a nicotine withdrawal symptom, and the activation of these neurons may act as a potential therapeutic strategy for preventing the somatic manifestations of nicotine withdrawal. (c) 2021 Delhi Orthopedic Association. All rights reserved.

Published
2021

83. Magnification endoscopy in combination with acetic acid enhancement and narrow-band imaging for the accurate diagnosis of colonic neoplasms

Author

SHIBAGAKI, Kotaro, Ishimura, Norihisa, Yuki, Takafumi, Taniguchi, Hideaki, Aimi, Masahito, Kobayashi, Keita, KOTANI, Satoshi, YAZAKI, Tomotaka, Yamashita, Noritsugu, Tamagawa, Yuji, MISHIRO, Tsuyoshi, Ishihara, Shunji, Yasuda, Akira, Kinshita, Yoshikazu, SHIBAGAKI, Kotaro, Ishimura, Norihisa, Yuki, Takafumi, Taniguchi, Hideaki, Aimi, Masahito, Kobayashi, Keita, KOTANI, Satoshi, YAZAKI, Tomotaka, Yamashita, Noritsugu, Tamagawa, Yuji, MISHIRO, Tsuyoshi, Ishihara, Shunji, Yasuda, Akira, and Kinshita, Yoshikazu

Published
2020

85. A Validation Study of the National Database of Health Insurance Claims and Specific Health Checkups of Japan Regarding the Annual Number of Tooth Extractions Performed Across the Entire Japanese Population

Author

Suzuki, Seitaro, primary, Sugihara, Naoki, additional, Kamijo, Hideyuki, additional, Morita, Manabu, additional, Kawato, Takayuki, additional, Tsuneishi, Midori, additional, Kobayashi, Keita, additional, Hasuike, Yoshihiro, additional, and Sato, Tamotsu, additional

Published
2021
Full Text
View/download PDF

87. Noninvasive measurement of heartbeat, respiration, snoring and body movements of a subject in bed via a pneumatic method

Author

Watanabe, Kajiro, Watanabe, Takashi, Watanabe, Harumi, Ando, Hisanori, Ishikawa, Takayuki, and Kobayashi, Keita

Subjects
Heart beat -- Measurement, Pneumatic tools -- Usage, Biological sciences, Business, Computers, Health care industry
Abstract

We have developed a noninvasive pneumatics-based system by which to measure heartbeat, respiration, snoring, and body movements of a subject in bed. A thin, air-sealed cushion is placed under the bed mattress of the subject and the small movements attributable to human automatic vital functions are measured as changes in pressure using a pressure sensor having an almost flat frequency response from 0.1 to 5 kHz and a sensitivity of 56 mV/Pa. Using the newly developed system, heartbeat, respiration, apnea, snoring and body movements are clearly measured. In addition, the optimal signal-to-noise (S/N) ratio by which to evaluate the reliability of the heart rate measurement is presented. Heart rates were measured for four different body postures, 13 different subjects, four different bed mattresses, and three different sensor positions. For these measurements, the S/N ratios ranged from 15.9 to 23.5 dB, and so were determined to be reliable. Index Terms--Body movements, health monitoring, heartbeat, noninvasive measurement, pressure sensor, respiration, snoring, vital sign.

Published
2005

94. Magnification endoscopy in combination with acetic acid enhancement and narrow-band imaging for the accurate diagnosis of colonic neoplasms

Author

Shibagaki, Kotaro, additional, Ishimura, Norihisa, additional, Yuki, Takafumi, additional, Taniguchi, Hideaki, additional, Aimi, Masahito, additional, Kobayashi, Keita, additional, Kotani, Satoshi, additional, Yazaki, Tomotaka, additional, Yamashita, Noritsugu, additional, Tamagawa, Yuji, additional, Mishiro, Tsuyoshi, additional, Ishihara, Shunji, additional, Yasuda, Akira, additional, and Kinshita, Yoshikazu, additional

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results