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619 results on '"Li, Haopeng"'

53. Tau and tubulin protein aggregation characterization by solid-state nanopore method and atomic force microscopy.

Author

Acharjee, Mitu C., Li, Haopeng, Rollings, Ryan, Ma, Bo, Tung, Steve, and Li, Jiali

Subjects
*TAU proteins, *ATOMIC force microscopy, *SINGLE molecules, *SOLUTION (Chemistry), *FOCUSED ion beams, *SILICON nitride
Abstract

In this study, a silicon nitride nanopore-based sensing system was used to measure tau and tubulin monomers and their aggregations in salt solution at a single molecule level. Nanopores (6–30 nm) were fabricated on silicon nitride membranes supported by silicon substrates using a combination of focused ion beam milling and ion beam sculpting. When a charged protein molecule in the salt solution passes through a nanopore driven by an applied voltage, the protein molecule increases pore resistivity, which induces an ionic current drop that can be measured. The current drop amplitude is directly proportional to the local excluded volume of the protein molecule in the nanopore. We measured the monomers and aggregations of tau and tubulin proteins at biased voltages from 60 to 210 mV in a solution of pH 7.0–10. Our results showed that (1) the nanopore method was able to differentiate tau and tubulin proteins in their monomer and aggregated forms by their excluded volumes; (2) the most probable aggregation form was dimer for α- and β-tubulin and pentamer for αβ tubulin plus tau under experimental conditions; (3) the protein excluded volumes measured by the nanopore method depended on the applied voltage, and this observation could be explained by the nonuniform charge distribution of proteins. The monomer and aggregated proteins were further analyzed using atomic force spectroscopy (AFM), and protein volumes estimated by AFM were consistent with nanopore results. [ABSTRACT FROM AUTHOR]

Published
2023
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62. Study on the Post-Irradiation Effects of Single-Use Protective Clothing for Medical Use

Author

WANG Jingjun;LI Bolong;SUN QiZhong;ZHANG Yue;LI Haopeng;LI Mengyuan;WANG Liancai;SHEN Yiling;DAI Pei

Subjects
single-use protective clothing for medical use, radiation sterilization, mechanical properties, filtering efficiency, Nuclear and particle physics. Atomic energy. Radioactivity, QC770-798
Abstract

In this paper the single-use protective clothing for medical use was sterilized by γ-ray irradiation. Compared with the non-irradiated control, the apparent morphology of PP melt-blown fiber and amboss had no fracture. According to the national standard of GB/T 19082-2009, the mechanical properties, resistance to synthetic blood penetration, resistance to water penetration and filtering efficiency were measured. The results showed that the post-irradiation effects did not decrease the resistance to synthetic blood penetration or water penetration. Moreover, the filtering efficiency remained higher than 99.8%. On the other hand, the mechanical properties were reduced significantly due to the degradation of poly chain of PP induced by γ-ray irradiation. It has been assured that the important properties of single-use protective clothing for medical use are still above the quality level after γ-ray irradiation sterilization process. which can supply reference for the produce single-use protection of clothing of medical purpose at high quality.

Published
2021
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67. An immunogenic cell death-related classification predicts prognosis and response to immunotherapy in kidney renal clear cell carcinoma

Author

Wang, Licheng, primary, Zhu, Yaru, additional, Ren, Zhen, additional, Sun, Wenhuizi, additional, Wang, Zhijing, additional, Zi, Tong, additional, Li, Haopeng, additional, Zhao, Yan, additional, Qin, Xin, additional, Gao, Dacheng, additional, Zhang, Libo, additional, He, Ziyang, additional, Le, Wei, additional, Wu, Qiang, additional, and Wu, Gang, additional

Published
2023
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82. Identification and validation of MSMB as a critical gene for prostate cancer development in obese people

Author

Chen, Xi, Li, Haopeng, Liu, Boya, Wang, Xin’an, Zhou, Weidong, Wu, Gang, and Xu, Chengdang

Subjects
Brief Communication
Abstract

Prostate cancer (PCA) is one of the most common types of cancer and can seriously endanger the health of older men. Obesity is prevalent all around the world and triggered by lots of factors such as diet, environment and fat metabolism disorder can cause many neoplasms, including PCA. Evidence suggests that genetic changes increase the risk of PCA and obesity. However, the specific obesity-related genes leading to PCA are unknown. Obesity-related genes associated with PCA were identified and analyzed though three public electronic databases: Gene Expression Omnibus, The Cancer Genome Atlas, and Chinese Prostate Cancer Genome and Epigenome Atlas. The effect of obesity-related genes in PCA were analyzed using clinical data from different databases, while associations with immune cells were determined by TIMER web tool. The expression and function of obesity-related genes were verified using clinical samples from obese patients with PCA and PCA cells. We found that four genes, MSMB, BMP5, THBS4, and POPDC3, may lead to PCA occurrence in patients with obesity. In Gene Expression Omnibus database, MSMB and BMP5 were downregulated, while THBS4 and POPDC3 were upregulated. This trend was mainly preserved in the other electronic databases. We also discovered MSMB and THBS4 can affect PCA progression, and all these genes were risk factors for castration-resistant prostate cancer. Moreover, MSMB can impact disease-free survival status of patients with PCA. These obesity-related genes were also correlated with immune cells and immune cell infiltration in PCA. We further uncovered that MSMB was downregulated in clinical PCA and castration-resistant prostate cancer samples from patients with obesity and MSMB decreased PCA cells proliferation. These results indicate that MSMB is essential for PCA development in people with obesity and can be a biomarker for predicting PCA occurrence and progression in obese people.

Published
2023

96. Macrophage Extracellular Traps Exacerbate Secondary Spinal Cord Injury by Modulating Macrophage/Microglia Polarization via LL37/P2X7R/NF-κB Signaling Pathway

Author

Zhang, Chengyi, primary, Guo, Dong, additional, Qiao, Hao, additional, Li, Jie, additional, Li, Jiaxi, additional, Yang, Yubing, additional, Chang, Su’e, additional, Li, Fengtao, additional, Wang, Dong, additional, Li, Haopeng, additional, He, Xijing, additional, and Wang, Fang, additional

Published
2022
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