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Postherpetic neuralgia (PHN) is the most common and severe complication of acute herpes zoster. Early treatment of herpes zoster neuralgia is of great significance to reduce the incidence of PHN. This retrospective study evaluated the efficacy and safety of the combination of high-voltage pulsed radiofrequency (PRF) and oxygen-ozone(OOne-hundred patients diagnosed with AZN were classified into two groups (high-voltage PRF group [HP group, n = 50] and high-voltage PRF combined with OPain intensity and sleep quality in both groups at all time points improved after treatment compared to before treatment (P 0.05). After 1 week and 1 month of treatment, NRS and PSQI scores in both groups decreased, but the differences were not statistically significant (P0.05). NRS, PSQI scores, and the dosages of gabapentin and tramadol decreased more significantly in the HPO group than those in the HP group after 3 months (P 0.05). The incidence of PHN was significantly lower in the HPO group than in the HP group (P 0.05). There were no significant differences in adverse events between the groups.High-voltage PRF is a safe and effective method for treating AZN. The combination of high-voltage PRF and O

Carotenoids are the general term of natural pigments. The formation of plant color is probably related to the components of carotenoids. As the yellow variety of celery, it is rich in the composition and content of carotenoids. However, the transcript profiling and roles of the genes related to carotenoids biosynthesis in yellow celery remain unclear. In this study, three yellow celery cultivars at different growth stages were used to analyze the content and composition of carotenoids and transcriptional changes of carotenoid biosynthesis-related genes. The lutein and β-carotene were detected in yellow celery cultivar, while α-carotene and lycopene were not detected. The contents of lutein and β-carotene were higher in leaf blades than in petioles. During the growth and development, the contents of lutein and β-carotene gradually decreased in celery. Compared with the other two cultivars, the contents of lutein and β-carotene were the highest in 'Huangtaiji' of 65 days after sowing (DAS) and 85 DAS and 'Liuhehuangxinqin' of 105 DAS, respectively. The expression levels of AgLCYB and AgPSY2 genes were significantly correlated with lutein and β-carotene contents. This work provided a reference for the further study on carotenoid metabolisms in yellow celery and also made sense on the way of cultivating yellow celery with high carotenoids content.

Sporotrichosis is a fungal disease of the human and other mammals, caused by a complex of Sporothrix schenckii. The disease follows the traumatic inoculation to lead to fixed lesions, regional lymphangitic lesions, or even disseminated lesions including internal involvement, which depends on host immunological status and strain virulence. In this work, we observed the role of CD4+ T cells apoptosis and conversion of Th1/Th2-type cytokines in the cellular immunity regulation on mice model sporotrichosis. The experiments showed that there was more CD4+ T cells apoptosis, by endogenous apoptosis signaling pathway (P < .05), and more conversions of Th1/Th2-type cytokines in more severe and longer duration groups (P < .05). Meanwhile, the trends of the conversions of Th1/Th2-type cytokines were almost consistent with the CD4 + T cell's apoptosis in the corresponding groups. These findings suggest that CD4+ T cells apoptosis and conversion of Th1/Th2-type cytokines are contributing to promoting the progress of sporotrichosis.

Artificial intelligence technologies such as machine learning have been applied to protein engineering, with unique advantages in protein structure, function prediction, catalytic activity, and other issues in recent years. Screening better mutants is still a bottleneck in protein engineering. In this paper, a new sequence-activity relationship method was analyzed for its application in improving the thermal stability of Aspergillus terreus (R)-ω-selective amine transaminase. The experimental data from 6 single-point mutated enzymes were used as a learning dataset to build models and predict the thermostability of 26 mutants. Based on digital signal processing (DSP), this method digitized the amino acid sequence of proteins by fast Fourier transform (FFT) and then established the best model applying partial least squares regression (PLSR) to screen out all possible mutants, especially those with high performance. In protein engineering, the innovative sequence activity relationship (ISAR) method can make a reasonable prediction using limited experimental data and significantly reduce the experimental cost. The half-life ( T 1 / 2 ) of (R)-ω-transaminase was fitted with the amino acid sequence by the ISAR algorithm, resulting in an R 2 of 0.8929 and a cvRMSE of 4.89. At the same time, the mutants with higher T 1 / 2 than the existing ones were predicted, laying the groundwork for better (R)-ω-transaminase in the later stage. The ISAR algorithm is expected to provide a new technique for protein evolution and screening.

Sporotrichosis is a fungal disease of the human and other mammals, caused by a complex of Sporothrix schenckii. The disease follows the traumatic inoculation to lead to fixed lesions, regional lymphangitic lesions, or even disseminated lesions including internal involvement, which depends on host immunological status and strain virulence. In this work, we observed the role of CD4

The paper was aimed to explore the role of serum exosomes induced by hepatic ischemia/reperfusion (I/R) injury in the damage of hippocampus and cerebral cortex of rats. The male Sprague-Dawley (SD) rats were randomly divided into 4 groups: sham operation group (S), hepatic I/R injury group (I/R), serum exosomes from S group treatment group (ES) and serum exosomes from I/R group treatment group (EI). In ES group and EI group, 100 μL serum exosomes from S group and I/R group were injected into the normal rats through tail vein respectively. Another three normal rats were injected intravenously with serum exosomes labeled with PKH26 red fluorescence, and then the expression of fluorescence in the brain tissues was observed by immunofluorescence microscope. The morphology and size of exosomes were observed by transmission electron microscope, the expression of exosomes markers CD63 and CD9 was detected by Western blot, and the damage of liver and brain, levels of apoptosis and oxidative stress response in hippocampus and cerebral cortex were observed by serological and histological indexes. The results showed that the exosomes were a group of round or ovoid membranous vesicles, sized in 30-100 nm. Compared with that in S group, the content of serum exosomes in I/R group was increased (P0.05). Moreover, serum exosomes could go through the blood-brain barrier and enter the brain tissue freely through blood circulation. The index of liver function in I/R group was significantly higher than that in S group (P0.05). There was no significance in the degree of brain damage, apoptosis and oxidative stress in hippocampus and cerebral cortex between S group and ES group. Compared with those in S group and ES group, the serum levels of brain injury markers, apoptosis index (AI) and oxidative stress in hippocampus and cerebral cortex increased in I/R group and EI group (P0.05). Whereas, compared with those in I/R group, the above indicators in EI group decreased (P0.05). Therefore, hepatic I/R injury can lead to the damage of hippocampus and cerebral cortex, and the increased serum exosomes induced by hepatic I/R plays an important role.

Generative adversarial networks (GANs) have made remarkable success in image generations. However, how to deal with the multi-domain particular scenes generation, which converts specific object images to different reasonable scene domians, is still an open problem. In this paper, we propose a multi-domain particular scene generation model named PSceneGAN (Particular Scene Generative Adversarial Nets) that is a novel dual-condition GAN. PSceneGAN is the first model to achieve one-to-many specific scene generation under the guidance of semantics using only one model. In addititon, we collect and label a novel high-quality clothing data set named DRESS and use it to verify our PSceneGAN through a challenging task. The results show that PSceneGAN not only accurately generates corresponding reasonable scene images according to input scene and semantic descriptions, but also achieves desired results in quantitative and qualitative evaluation, among which frechet inception distance (FID) and inception score (IS) are 25.40 and 36.24, respectively.

ζ-Carotene desaturase (ZDS) is one of the key enzymes regulating carotenoids biosynthesis and accumulation. Celery transgenic efficiency is low and it is difficult to obtain transgenic plants. The study on ZDS was limited in celery. Here, the AgZDS gene was cloned from celery and overexpressed in Arabidopsis thaliana and celery to verify its function. The AgZDS has typical characteristic of ZDS protein and is highly conserved in higher plants. Phylogenetic analysis showed that AgZDS has the closest evolutionary relationship with ZDSs from Solanum lycopersicum, Capsicum annuum and Tagetes erecta. Overexpression of AgZDS gene in A. thaliana and celery resulted in increased accumulations of lutein and β-carotene and up-regulated the expression levels of the genes involved in carotenoids biosynthesis. The contents of lutein and β-carotene in two lines, AtL1 and AgL5, were the highest in transgenic A. thaliana and celery, respectively. The relative expression levels of 5 genes (AtPDS, AtZISO, AtZEP, AtNCED3, and AtCCD4) were up-regulated compared to the wild type plants. The relative expression levels of most genes in carotenoids biosynthesis pathway, such as AgPDS, AgCRTISO1, and AgZISO, were up-regulated in transgenic celery plants. The antioxidant capacity of A. thaliana and photosynthetic capacity of celery were also enhanced. This research is the first report on the function of structure gene related to carotenoid biosynthesis in transgenic celery plants. The findings in this study demonstrated the roles of AgZDS in regulating carotenoids metabolism of celery, which laid a potential foundation for quality improvement of celery.

Bentonite has been purified by suspension method and sodiumed with sodium carbonate, and then modified with polyaniline. Finally, characterized by the polarizing microscope, the X-ray diffractometer(XRD) and Fourier infrared spectrometer (FT-IR), in order to determine the optimal preparation technology of polyaniline/bentonite. And the optimal preparation of PAn/MMT is used to research the adsorption dynamics analysis on phenol. The result shows that the sodium ion enters into the bentonite interlayer through ion exchange; When the dosage of bentonite is 5 g, the dosage of FeCl3·6H2O is 2.7 g, the dosage of aniline is 46.5 g, and the dosage of DBSA is 3.26 g, its layer spacing of modified bentonite is 2.823nm, increaseing 1.600nm than the first, so the hydrophobicity and separation effects of the modified bentonite get greatly improvement. And the dosage of PAn/MMT is 0.1 g and the initial concentration of phenol、P-nitrophenol、o-cresol is 10 mg/L, their adsorption rates reach to 51.50%, 80.56%, 62.93%, more high than the sodium bentonite under the same conditinns. It indicates that the modified bentonite is more suitable for the adsorption of benzene homologs.

Oncogenic alterations in the BRAF gene are identified in an estimate of 50% of melanomas and cause melanoma development. BRAF kinase inhibitors (BRAFi), including vemurafenib and dabrafenib, were discovered and used in the clinical treatment of BRAF-mutant metastatic melanoma. Though, BRAFi's therapeutic advantages are short term and short-lived associated with drug resistance. Although a few pathways of developed BRAFi resistance have also been established, in approximately 40% of melanomas, the cause for inherited resistance remains unclear. Recognizing a new process of developed BRAFi resistance might provide new possibilities to successfully treat BRAF mutant melanoma. In this study, we are exploring the compensatory alternative pathway followed by BRAFi/MEKi treated resistant cell for maintaining the long-term integrity and survival.

International audience; One of the most widely used training methods for large-scale machine learning problems is distributed asynchronous stochastic gradient descent (DASGD). However, a key issue in its implementation is that of delays: when a "worker" node asynchronously contributes a gradient update to the "master", the global model parameter may have changed, rendering this information stale. In massively parallel computing grids, these delays can quickly add up if a node is saturated, so the convergence of DASGD is uncertain under these conditions. Nevertheless, by using a judiciously chosen quasilinear step-size sequence, we show that it is possible to amortize these delays and achieve global convergence with probability 1, even under polynomially growing delays, reaffirming in this way the successful application of DASGD to large-scale optimization problems.

To investigate macular thickness (MT) in young myopic Chinese patients using Spectralis optical coherence tomography (OCT) and to assess its association with gender, axial length (AL), spherical equivalent (SE), and intraocular pressure (IOP).Included in this study were 133 myopic Chinese patients, aged 18-30 years. All underwent complete ophthalmic examinations and Spectralis OCT. Inner, central, and outer foveal regions of the macula were measured.The central fovea was the thinnest of the three macular regions (mean thickness, 250.1 ± 15.3 μm). Women had consistently thinner MTs than did men, except in the outer superior and inferior areas. Central foveal thickness (CFT) was positively correlated with AL and negatively correlated with SE. In the inner and outer regions, MT was positively correlated with SE. AL was negatively correlated with MT in all quadrants of the inner and outer sectors, but not the inner superior and outer nasal areas. No macular measurement was significantly correlated with age or IOP in either gender.Variables affecting MT should be considered when evaluating SD-OCT data in young myopic Chinese patients, with and without retinal diseases.

Robust low-level image features have been proven to be effective representations for a variety of visual recognition tasks such as object recognition and scene classification; but pixels, or even local image patches, carry little semantic meanings. For high level visual tasks, such low-level image representations are potentially not enough. In this paper, we propose a high-level image representation, called the Object Bank, where an image is represented as a scale-invariant response map of a large number of pre-trained generic object detectors, blind to the testing dataset or visual task. Leveraging on the Object Bank representation, superior performances on high level visual recognition tasks can be achieved with simple off-the-shelf classifiers such as logistic regression and linear SVM. Sparsity algorithms make our representation more efficient and scalable for large scene datasets, and reveal semantically meaningful feature patterns.

The rheological property of fresh high-strength concrete (HSC) and mechanical properties, self-shrinkage and resistance to chloride ion permeability of hydrated HSC were studied on the basis of the closest packing theory. Results showed that the packing density of composite powder was the highest when the content of microsphere was 30.0%; when the content of zeolite powder added to concrete varied from 0.0% to 4.0%, the rheological and discontinuous property of concrete would be improved and its viscosity would be increased; meanwhile, compared to the standard concrete, the self-shrinkage of concrete with 2.0% zeolite powder at 1d, 3d, 7d and 28d was decreased by 77.1%, 49.6%, 44.0% and 38.1% respectively; finally, the resistance to chloride ion permeability of hydrated HSC with zeolite powder was improved with a result of 18.5% reduction of electric flux at 28d.

The paper studied the effects of single addition of ultrafine fly ash (UFA) and mixed addition of silica fumeultrafine fly ash (SF-UFA) on powder pressure entity dense packing density, mechanical performance and microstructure structure of composite cement paste. The results show that, the ternary system of mixed addition of SF-UFA can effectively further reduced the compacting voidage and more increased packing density of composite powder, compared with the binary system of single addition of UFA. And when the content of SF is 8%, the compacting voidage is the lowest. Next, in the ternary system of mixed with silica fume, ultrafine fly ash and cement (SF-UFA-C), with the increase of silica fume (SF), the total porosity and pore diameter of hardened cementing paste decreased further compared with binary system, and with 8% content of SF and 17% content of UFA, the total porosity of hardened cement paste is the lowest, decreased by 60% than that of reference sample. At last, mixed addition of SF-UFA can improve the long-term strength of composite cement paste either, the compressive strength of 60 days hydration increased by nearly 9% compared with UFA-C and increased 41% compared with the reference sample.

The expansion of cement mortar mixing expansive agent under standard curing and membrane curing conditions and compressive strength in constrained conditions are studied in this paper. Results show that the early expansion effect of calcium sulphoaluminate hydrate-calcium hydroxide expansive agent is obvious, mid-to late effect of magnesia expansive agent increase clearly. Compare with standard curing, the shrinkage of the basic cement mortar increase and expansion effect of expansive agent reduce under membrane curing. The expansion effect of expansive agent B is good and persistent under membrane curing, its applicable to used in the closed environment, such as concrete filled steel tubes. The compressive strength of mortar with expansion agent is a little more than the basic mortar under the condition of limit.

High-rise building with podium is a common seen architecture style in China. Nowadays more designers inclined not to separate the main and podium, but this would lead to the eccentric between the center of mass of the tower and base, which may exceed the code requirement when the ratio of offsetting is larger than 20% and cause complicated seismic response. This paper takes an eccentric high-rise frame-shear wall structure of single tower as example, analyze the inelastic performance of this structure under rare earthquake by Pushover method, and point out the weak parts of the position by the distribution and development of plastic hinges.Draw the structure inelastic response indicators in the rare case of earthquakes, and point out the weak parts of the structure of the distribution and development of plastic hinge (region), put forward reasonable proposals for optimizing the structure design.

An interference imaging system with annular aperture is presented having focal length of 6000 mm, aperture of 1000 mm, and ring width/semi-diameter of 0.2. The energy analyze is carried out to evaluate the optical performance of this system, and imaging restoration processing is also performed by the blind deconvolution algorithm with iteration number of 25. Results show that the quality is close to the diffraction limit and the weight has greatly reduced to 36% of the full-aperture system, which verifies the annular aperture system is a better choice for the space telescope with large-caliber and long focal length.

Mining of the upper protective coal seam is widely practiced in China for coal mine safety, but relief gas may present a new risk of blasting. To control the relief gas effectively, a strain-soften model was built by FLAC3D software to investigate the stress evolution during the process of mining the upper protective coal seam. The results show that the abutment stress changes rapidly within 10 m in front of the coal face, and the maximum abutment stress is approximately twice the original when the coal seam is mined 20–30 m. The abutment stress should break the rock mass and cause the gas to flow easily. In the stable mining period, the change trends of the x-stress and z-stress are different, and these should also pre-break the rock mass. The stress distributions of the rock mass at different distances under the protective coal seam are different, especially near the coal face, which should greatly affect the gas flow when the space of the protective and protected coal seams change over a large range. The relief angle also changes over a large range, increasing to a maximum approximately 30 m behind the coal face, and it decreases gradually when it is far away from the protective coal seam. The results are helpful for designing the coal face of protected coal seams and borehole layouts to control the relief gas.

Ordinary Portland cement in corrosive environment such as saline-alkali land, sea water and chemical pollution can easily be corroded, thus cause infrastructure damage. In this paper, N-vinyl pyrrole was added into cement mortar, to improve sulphate erosive resistance of cement material. Through strength testing, quick sulfate corrosion test, XRD and SEM analysis methods in experiment, the researching results have shown that NVP can effectively improve the property of cement.

Preparation of advanced early strength agent ,the main components including organic A, organic B and calcium nitrate. Then tested advanced early strength agent performance for concrete. Though text, we find, the compressive strength and flexural strength of the specimen, use of the advanced early strength agent is greatly improved than with its much better not to use advanced early strength agent. With the passage of time, advanced early strength agent added does not make the strength of concrete worse, but better.Use SEM and XRD analysis technology text advanced early strength agent by adding performances of concrete, including the degree of cement hydration, the morphology of hydration products and the composition of mineral formation. analysis of the role of advanced early strength agents from the perspective of the mechanism. Identified advanced early strength agent impact on the cement hydration, for the further development of early strength agent to provide a theoretical basis.

In this paper, an equation correlated with normal stress and permeability was developed and FLAC3D software was used to investigate the rock mass stress evolution and distribution to understand the methane flow characteristics. Research results show that the rock mass under the protective coal seam can be divided into three belts (zones) in the vertical direction, including total de-stressed belt, vertical de-stressed belt and original stress belt. Methane in the total de-stressed belt can flow into the working face of protective coal seam by its own pressure gradient. Methane in the vertical de-stressed belt can only be extracted by boreholes. In the horizontal direction, the rock mass was also divided into five zones, including original zone, compression zone, expansion zone, recovering zone and re-compacted zone, which have been proved correct by the field experiments. The rock mass permeability in the de-stressed belts doesn't increase until stepping into the expansion zone, and from then on higher concentration of methane can be extracted. The methane in original regions is difficult to extract because rock stresses stay the same. The division of “three belts and five zones” reveals the rock mass stress and permeability distribution and evolution and supplies theoretical guidance for relieved methane control.

Objectives: This study aimed to investigate whether the sunscreen-containing 2–5% green tea extracts (GTEs) protect ultraviolet irradiation (UVR)-induced photoaging and photoimmunosuppression. Materials and methods: Twenty volunteers were exposed to repetitive solar-simulated UVR (ssUVR) on the upper back at a dosage of 1.5 minimal erythema doses (MED) per day for four consecutive days. Thirty minutes before each UVR and 6, 24, and 48 h after the last UV exposure, the products containing vehicle, and 2–5% GTEs were applied onto five sites on the dorsal skin, respectively. The skin biopsies were obtained 72 h after the last UVR. The thickness of the stratum corneum and epidermis was measured under the microscope and the expression of cytokeratins (CK)-5/6, CK16, metalloproteinases (MMP)-2, MMP-9, and the CD1a+ Langerhans cells (LCs) were determined using immunohistochemistry. Results: Our results showed that UVR substantially induced cutaneous erythema, thickening of the epidermis, overexpression of CK5/6, CK16, MMP-2, MMP-9, and depletion of CD1a+ LCs. The sunscreens containing different concentrations of GTEs conferred significant protection against the photoaging and photoimmunology-related biological events. Interestingly, the protective effects were not parallel to the concentrations of GTEs, with 2% and 3% GTEs showing the most efficacious photoprotection. Conclusions: GTEs-containing sunscreens have potential photoprotective effects on UVR-induced photoaging and photoimmunosuppression.

To investigate the effects of Ku80 depletion on cell growth and sensitization to gamma-radiation and MMC-induced apoptosis in esophageal squamous cell carcinoma lines. Six human carcinoma cell lines (LNcaP, K562, MDA-MB-231, MCF-7, EC9706, and K150) and normal HEK293 cell line were examined for basal levels of Ku80 protein by western blotting analysis. The suppression of Ku80 expression was performed using vector-based shRNA in EC9706 cells. Cell proliferation was determined with MTT assay and colony formation assay and tumorigenicity in a xenograft model in vitro and in vivo. Sensitivity of EC9706 cells treated with shRNA vector to gamma-radiation and MMC was determined with colony formation assay and MTT assay. The cell cycle distribution was determined by Flow cytometry. Apoptosis induced by gamma-radiation and MMC was analyzed using GENMED-TUNEL FACS kit. Ku80 showed higher basal levels in six carcinoma cell lines than in HEK293. The suppression of Ku80 expression decreased cellular proliferation, colony formation and inhibited tumorigenicity in a xenograft model. Furthermore, it sensitized apoptosis of the cancer cells induced by gamma-radiation and MMC. Ku80 plays an important role not only in tumorigenesis but also in radiation resistance and chemotherapy resistance in esophageal cancer cells. Hence Ku80 may serve as a promising therapeutic target, particularly for recurrent esophageal tumors.

Concrete Design of concrete filled steel tube should not only meet the requirements of self-compacting, but also need suitable expansion properties. On the basis of working performance requirement, study impact of expansive agent on concrete working performance, strength and expansion properties, preparation of C60 concrete filled steel to meet the self-compacting and expansion properties. Expander should not be too much, otherwise working performance and strength will be affected. Meanwhile study the correlation between restrained expansion and free expansion of expansive concrete, and analyze the mechanism of expansive concrete.