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Wheat (Triticum aestivum L.) wild relatives are rich genetic resources for genetic improvement of wheat. The short arm of rye (Secale cereale L.) chromosome 1 (1RS) contains many beneficial genes for resistance to drought, insects, and diseases, therefore the 1RS wheat translocations have been widely used in wheat breeding programs worldwide. To facilitate marker‐assisted identification of 1RS in wheat breeding, we designed a set of Kompetitive allele‐specific polymerase chain reaction (KASP) markers to detect the translocations of 1RS with long arm from wheat chromosome 1A (1RS.1AL) and 1RS with long arm from wheat chromosome 1B (1RS.1BL) based on the sequence flanking single nucleotide polymorphisms (SNPs) generated by genotyping‐by‐sequencing (GBS) between wheat and rye panels. We identified a set of three SNP markers for diagnosis and differentiation of the two 1RS translocations in wheat. Among the three KASP markers, 1AS‐05056 is 1RS specific, and clearly separated the genotypes with 1RS (1RS.1AL and 1RS.1BL) from those without the two 1RS translocations; whereas 1AS‐29875 clearly identified the 1RS.1AL translocation and 1BS‐16654 separated 1RS.1BL from 1RS.1AL. The three new KASP markers together accurately identified the presence of 1RS translocation on either 1A or 1B chromosome in a panel of 95 representative U.S. winter wheat accessions, which were further validated in two additional U.S. hard winter wheat panels. Therefore, they can be used for routine screening of 1RS.1BL and 1RS.1AL translocation lines in wheat breeding programs. Core Ideas: 1RS, 1AS, and 1BS specific SNPs were identified by analyzing GBS‐SNPs from both wheat and rye panels.Three chromosome‐arm‐specific KASPs clearly detected the 1RS.1AL and 1RS.1BL translocations in wheat.Three KASP markers were diagnostic for 1RS.1AL and 1RS.1BL in two U.S. hard winter wheat panels. [ABSTRACT FROM AUTHOR]

In order to use single top flavors with roasted sweet aroma in heated tobacco products for flavor enhancement, puff-by-puff release of six roasted sweet aroma compounds in the aerosols of heated tobacco products was measured using GC-MS. The six aroma components were added into the tobacco rod at 0.01% and 0.05% of tobacco mass, and the puff-by-puff transfer rates were determined. The results showed that: 1) The puff-by-puff releases of the six aroma components in the aerosol of heated tobacco products increased first and then decreased with the puff number. 2) When added at 0.01% and 0.05%, their puff-by-puff transfer rates were between 0.5%-15.6% and 1.0%-9.6%, respectively, and the per puff transfer rate peaked in the third or fourth puff. 3) There was a good linear positive correlation between the total of puff-by-puff transfer rates of the six single flavors in aerosols and the puff number. 4) At the two addition rates, the total transfer rates of the six single flavors in aerosols were between 34.2%-73.1% and 23.3%-63.7% respectively. [ABSTRACT FROM AUTHOR]

In order to investigate the effects of cigarette paper properties on the combustion coal fallout propensity of burning slim cigarette, cigarette paper with different parameters ( grammage, air permeability, hemp pulp content, ash content, burn promoter content and proportion of potassium salts in burn promoter) was selected and the samples of fine cigarette were made. The parameters were set optimization. The results showed that the combustion coal fallout propensity of slim cigarettes decreased rapidly with the increase of paper grammage. The presence of air permeability, hemp pulp content and ash content in cigarette papers was able to promote the fallout propensity of combustion coal, in a content-dependent manner. Meanwhile, air permeability of cigarette paper exhibited a positive correlation to the combustion coal fallout propensity. The change of burn promoter content had no significant effect on reducing the tendency of the fallout propensity of combustion coal, while the excessively high proportion of potassium salts in burn promoter would cause the tendency of the fallout propensity of combustion coal to fall rapidly. The four parameters of air permeability, grammage, burn promoter content and proportion of potassium salts in burn promoter were sorted in descending order of the influence on the combustion coal fallout propensity as below: air permeability > grammage > proportion of potassium salts in burn promoter > burn promoter content. The combustion coal fallout propensity decreased from6. 3% to 2.5% with the optimum cigarette paper parameters of air permeability 50 CU, grammage 34 ⋅ m-2, proportion of potassium salts in burn promoter 50%, burn promoter content 1.5%. [ABSTRACT FROM AUTHOR]

BACKGROUND Understanding the variance of antioxidant in wheat grain responses to irrigation and nitrogen (N) fertiliser management will improve the nutrient quality of wheat grain. Four N rates (0, 180, 240, and 300 kg ha−1) combined with irrigation times (I0, no irrigation; I1, jointing time irrigation; I2, jointing + flowering time irrigation), were used to determine the effect of N fertilisation and irrigation on total phenolic content (TPC), phenolic acid composition, and antioxidant activity (AOA) of wheat grain. RESULTS Irrigation, N fertilisation and their interactions had significant effect on TPC, total flavonoid content (TFC), AOA, p-coumaric acid (PCA), as well as vanillic acid (VA) and chlorogenic acid (CA). I1N300 treatment had the highest TPC at Zhengzhou and Wenxian (1451.5 µg g−1 and 1397.9 µg g−1, respectively) location, while I1N240 resulted in the highest TFC (0.75 mg g−1) and VA (19.77 µg g−1) at Wenxian. TPC, TFC, AOA, ferulic acid (FA), PCA and VA increased with N application rate (from 180 to 300 kg N ha−1). CONCLUSION An appropriate irrigation and N management improved antioxidant content and AOA in wheat grain. Generally, I1N240 and I1N300 treatment resulted in the higher TPC, TFC, AOA, as well as phenolic acid, i.e. FA and VA. © 2014 Society of Chemical Industry [ABSTRACT FROM AUTHOR]