149 results on '"Lidan, Xu"'
Search Results
52. Look at Me! Or Don’t… : How Mere Social Presence Impacts Innovation Adoption
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Ravi Mehta, Kelly B. Herd, and Lidan Xu
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Marketing ,Social risk ,Economics and Econometrics ,0502 economics and business ,05 social sciences ,050211 marketing ,Uniqueness ,Business ,Innovation adoption ,050203 business & management ,Applied Psychology - Abstract
While the adoption of innovative products offers an opportunity for consumers to stand out and signal their uniqueness, such adoption also may also be associated with social risk. The curre...
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- 2019
53. Association between polymorphisms in MRE11 and HIV-1 susceptibility and AIDS progression in a northern Chinese MSM population
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Songbin Fu, Haiming Sun, Jie Wu, Xueyuan Jia, Jiawei Wu, Kaili Wang, Chang Liu, Qingbu Mei, Qiuyan Li, Yuandong Qiao, Lidan Xu, Xuelong Zhang, and Wenjing Sun
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Adult ,Male ,0301 basic medicine ,Microbiology (medical) ,China ,Adolescent ,Genotype ,030106 microbiology ,Population ,HIV Infections ,Single-nucleotide polymorphism ,Biology ,Polymorphism, Single Nucleotide ,Linkage Disequilibrium ,Young Adult ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Gene Frequency ,Odds Ratio ,Humans ,Genetic Predisposition to Disease ,Pharmacology (medical) ,030212 general & internal medicine ,Homosexuality, Male ,Allele ,education ,Allele frequency ,Gene ,Alleles ,Aged ,Aged, 80 and over ,Pharmacology ,Genetics ,Acquired Immunodeficiency Syndrome ,MRE11 Homologue Protein ,education.field_of_study ,Haplotype ,Middle Aged ,Viral Load ,Infectious Diseases ,Bonferroni correction ,Haplotypes ,MRN complex ,Case-Control Studies ,HIV-1 ,symbols - Abstract
BACKGROUND Previous studies reported that DNA damage repair (DDR) genes may play an important role in HIV-1 infection. The MRE11 gene, a member of the MRN complex, plays an essential part in the homologous recombination pathway, which is one of the classical DDR pathways. Previous reports have demonstrated that MRE11 has an effect on HIV-1 replication. However, the role of SNPs in the MRE11 gene and their impact on HIV-1 infection and AIDS progression remain unknown. METHODS In this study, 434 MSM HIV-1-infected patients in northern China and 431 age-matched healthy controls were enrolled. Five SNPs (rs2155209, rs10831234, rs13447720, rs601341 and rs11020803) at the MRE11 gene were genotyped. Another series of cases (409 MSM HIV-1-infected patients) and controls (403 age-matched healthy males) were recruited as the validation set. RESULTS In our study, rs10831234 showed differences in allele frequencies between cases and controls (P = 0.005). Additionally, there was an association between rs10831234 and HIV-1 infection susceptibility in dominant and additive models (P = 0.005 and P = 0.006, respectively). All significant associations were replicated in the validation set, and the associations were still significant after Bonferroni correction for multiple testing when the two data sets were combined. Furthermore, in haplotype association analyses between the case and control groups, the frequencies of the haplotypes Crs11020803Crs10831234 and Trs11020803Trs10831234 showed significant differences (P = 0.0181 and P = 0.0068, respectively). CONCLUSIONS We demonstrated that the MRE11 rs10831234-T allele may confer increased risk of HIV-1 infection.
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- 2019
54. Retrieval model for total nitrogen concentration based on UAV hyper spectral remote sensing data and machine learning algorithms – A case study in the Miyun Reservoir, China
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Qun'ou, Jiang, primary, Lidan, Xu, additional, Siyang, Sun, additional, Meilin, Wang, additional, and Huijie, Xiao, additional
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- 2021
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55. Disease causing property analyzation of variants in 12 Chinese families with polycystic kidney disease
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Rongwei Guan, Jie Wu, Jing Bai, Hui Su, Guohua Ji, Peng Liu, Kexian Dong, Songbin Fu, Xueyuan Jia, Yun Huang, Xianli Zhou, Wenjing Sun, Zhang Xuelong, Xiaogang Liu, Wei Ji, Lidan Xu, and Huanhuan Miao
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Adult ,Male ,0301 basic medicine ,Candidate gene ,medicine.medical_specialty ,TRPP Cation Channels ,lcsh:QH426-470 ,Autosomal dominant polycystic kidney disease ,Consanguinity ,030105 genetics & heredity ,Biology ,03 medical and health sciences ,symbols.namesake ,Gene Frequency ,Protein Domains ,autosomal dominant ,Genetics ,Polycystic kidney disease ,medicine ,Humans ,inheritance ,Molecular Biology ,Genetics (clinical) ,Exome sequencing ,Sanger sequencing ,Polycystic Kidney Diseases ,novel variants ,polycystic kidney disease ,PKD1 ,pedigree ,Original Articles ,Middle Aged ,medicine.disease ,lcsh:Genetics ,030104 developmental biology ,Mutation ,symbols ,Medical genetics ,Female ,Original Article - Abstract
Background Polycystic kidney disease (PKD) is an inherited disease that is life‐threatening. Multiple cysts are present in the bilateral kidneys of PKD patients. The progressively enlarged cysts cause structural damage and loss of kidney function. Methods This study examined and analyzed 12 families with polycystic kidney disease. Whole exome sequencing (WES) or whole genome sequencing (WGS) of the probands was performed to detect the pathogenic genes. The candidate gene segments for lineal consanguinity in the family were amplified by the nest PCR followed by Sanger sequencing. The variants were assessed by pathogenic and conservational property prediction analysis and interpreted according to the American College of Medical Genetics and Genomics. Results Nine of the 12 pedigrees were identified the disease causing variants. Among them, four novel variants in PKD1, c.6930delG:p.C2311Vfs*3, c.1216T>C:p.C406R, c.8548T>C:p.S2850P, and c.3865G>A:p.V1289M (NM_001009944.2) were detected. After assessment, the four novel variants were considered to be pathogenic variants and cause autosomal dominant polycystic kidney disease in family. The detected variants were interpreted. Conclusion The four novel variants in PKD1, c.6930delG:p.C2311Vfs*3, c.1216T>C:p.C406R, c.8548T>C:p.S2850P, and c.3865G>A:p.V1289M (NM_001009944.2) are pathogenic variants and cause autosomal dominant polycystic kidney disease in family., This study examined and analyzed 12 families with polycystic kidney disease using whole exome sequencing or whole genome sequencing and several prediction tools. After assessment and interpretion, the four novel variants were considered to be pathogenic variants and cause autosomal dominant polycystic kidney disease in family. The present study enriched the knowledge of the pathogenicity for autosomal dominant polycystic kidney disease and would improve the understanding of this autosomal dominant disorder.
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- 2020
56. Association Between Nonsyndromic Cleft Lip and Palate and 2 Polymorphic Loci: A Meta-Analysis
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Jie Wu, Yuandong Qiao, Wenjing Sun, Yusi Wang, Xueyuan Jia, Ping Wang, Qiuyan Li, Lidan Xu, and Xuelong Zhang
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0301 basic medicine ,Genetics ,biology ,business.industry ,Cleft Lip ,Single-nucleotide polymorphism ,030105 genetics & heredity ,Polymorphism, Single Nucleotide ,Cleft Palate ,03 medical and health sciences ,030104 developmental biology ,Otorhinolaryngology ,Methylenetetrahydrofolate reductase ,Meta-analysis ,Case-Control Studies ,biology.protein ,Medicine ,Humans ,Genetic Predisposition to Disease ,Polymorphic locus ,Oral Surgery ,Noggin ,Craniofacial ,business - Abstract
Objectives: The relationship between Noggin ( NOG) and methylenetetrahydrofolate reductase and nonsyndromic cleft lip and palate (NSCLP) has been reported participate in craniofacial development but need further evidence. To indicate the susceptibility between the 2 genes and NSCLP, rs227731 and rs1801131 polymorphisms were included in the present research. This research may provide some genetic clues for disease detection and surveillance. Design: Seventeen studies including 4023 cases and 5691 controls were provided for meta-analysis, and odds ratio (OR) with 95% CI were obtained to estimate NSCLP risk. Results: Our analysis suggested potential association of rs227731C on increasing the risk of NSCLP in the Caucasian group and total group but not Asian group under all models: allele (OR = 1.45, 95% CI = 1.21-1.75, P < .0001), homozygote (OR = 2.03, 95% CI = 1.42-2.90, P < .0001), heterozygote (OR = 1.44, 95% CI = 1.19-1.73, P = .0001), dominant (OR = 1.61, 95% CI = 1.27-2.04, P < .0001), and recessive models (OR = 1.63, 95% CI = 1.25-2.12, P = .0003). Besides, increased risk is related to rs1801131 in Asian group under 3 models: allele (OR = 1.24, 95% CI = 1.06-1.44, P = .006), heterozygote (OR = 1.24, 95% CI = 1.02-1.52, P = .03), and dominant models (OR = 1.29, 95% CI = 1.06-1.56, P = .009). Conclusions: Our analysis indicates polymorphisms rs227731 and rs1801131 are associated with NSCLP, with predominance of different ethnic group and deepen understanding of NSCLP.
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- 2020
57. Spatial Structure of a Potential Ecological Network in Nanping, China, Based on Ecosystem Service Functions
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Li Cui, Ling Xiao, Qun’ou Jiang, Haiming Yan, Lidan Xu, and Meilin Wang
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010504 meteorology & atmospheric sciences ,Biodiversity ,landscape connectivity ,010501 environmental sciences ,01 natural sciences ,Ecosystem services ,lcsh:Agriculture ,Urbanization ,ecosystem service function ,ecological network ,0105 earth and related environmental sciences ,Nature and Landscape Conservation ,Global and Planetary Change ,Ecology ,business.industry ,Environmental resource management ,least-cost path ,Fragmentation (computing) ,lcsh:S ,Ecological network ,Nanping City ,Geography ,Habitat ,Common spatial pattern ,business ,Landscape connectivity - Abstract
The increasing scale of urbanization and human activities has resulted in the fragmentation of natural habitats, leading to the reduction of ecological landscape connectivity and biodiversity. Taking Nanping as the study area, the core areas with good connectivity were extracted as ecological sources using a morphological spatial pattern analysis (MSPA) and landscape connectivity index. Then the ecosystem service functions of the ecological sources were evaluated based on the InVEST model. Finally, we extracted the potential ecological corridor based on the land type, elevation and ecosystem service functions. The results showed that the ecological source with higher landscape connectivity is distributed in the north and there are clear landscape connectivity faults in the northern and southern regions. Moreover, the areas with high habitat quality, soil retention and water production are mainly distributed in the northern ecological source areas. The 15 potential ecological corridors extracted were distributed unevenly. Among them, the important ecological corridors formed a triangle network, while the general ecological corridors were concentrated in the northwest. Therefore, it is suggested that the important core patches in the north be protected, and the effective connection between the north and south be improved. These results can provide a scientific basis for ecological construction and hierarchical management of the ecological networks.
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- 2020
58. Air pollution and DNA methylation in adults: A systematic review and meta-analysis of observational studies
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Chunmei Guo, Quanman Li, Yanyan Zhang, Shengbing Huang, Min Cheng, Minghui Han, Yang Zhao, Xingjin Yang, Fulan Hu, Yang Li, Xiaoyan Wu, Dechen Liu, Qionggui Zhou, Gang Tian, Yuying Wu, Pei Qin, Yifei Feng, Lidan Xu, Dongsheng Hu, Ranran Qie, Yunhong Zeng, and Ming Zhang
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Genetics ,Candidate gene ,Air Pollutants ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,Locus (genetics) ,General Medicine ,Methylation ,Environmental Exposure ,010501 environmental sciences ,Biology ,DNA Methylation ,Toxicology ,01 natural sciences ,Pollution ,Observational Studies as Topic ,Differentially methylated regions ,CpG site ,Meta-analysis ,Air Pollution ,DNA methylation ,Particulate Matter ,0105 earth and related environmental sciences ,Genetic association - Abstract
This systematic review and meta-analysis aimed to investigate the association between air pollution and DNA methylation in adults from published observational studies. PubMed, Web of Science and Embase databases were systematically searched for available studies on the association between air pollution and DNA methylation published up to March 9, 2021. Three DNA methylation approaches were considered: global methylation, candidate-gene, and epigenome-wide association studies (EWAS). Meta-analysis was used to summarize the combined estimates for the association between air pollutants and global DNA methylation levels. Heterogeneity was assessed with the Cochran Q test and quantified with the I2 statistic. In total, 38 articles were included in this study: 16 using global methylation, 18 using candidate genes, and 11 using EWAS, with 7 studies using more than one approach. Meta-analysis revealed an imprecise but inverse association between exposure to PM2.5 and global DNA methylation (for each 10-μg/m3 PM2.5, combined estimate: 0.39; 95% confidence interval: 0.97 - 0.19). The candidate-gene results were consistent for the ERCC3 and SOX2 genes, suggesting hypermethylation in ERCC3 associated with benzene and that in SOX2 associated with PM2.5 exposure. EWAS identified 201 CpG sites and 148 differentially methylated regions that showed differential methylation associated with air pollution. Among the 307 genes investigated in 11 EWAS, a locus in nucleoredoxin gene was found to be positively associated with PM2.5 in two studies. Current meta-analysis indicates that PM2.5 is imprecisely and inversely associated with DNA methylation. The candidate-gene results consistently suggest hypermethylation in ERCC3 associated with benzene exposure and that in SOX2 associated with PM2.5 exposure. The Kyoto Encyclopedia of Genes and Genomes (KEGG) network analyses revealed that these genes were associated with African trypanosomiasis, Malaria, Antifolate resistance, Graft-versus-host disease, and so on. More evidence is needed to clarify the association between air pollution and DNA methylation.
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- 2020
59. Genetic association of G2/M checkpoint genes with susceptibility to HIV-1 infection and AIDS progression in northern Chinese MSM population
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Jiawei Wu, Xuelong Zhang, Kaili Wang, Lidan Xu, Bangquan Liu, Wenjing Sun, Yuanting Hu, Xueyuan Jia, Haiming Sun, Jie Wu, Yun Huang, Wei Ji, Komal Saleem, Songbin Fu, and Yuandong Qiao
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virus diseases ,biological phenomena, cell phenomena, and immunity - Abstract
Background MSM has a high risk of HIV infection. Previous studies showed that the cell cycle regulation played an important role in HIV-1 infection, especially in G2/M checkpoint. The ATR, Chk1, Cdc25C and CDK1 are key genes in G2/M checkpoint. However, the association between the SNPs of these genes and susceptibility to HIV-1 infection and AIDS progression remains unknown. Results 42 tSNPs of G2/M checkpoint genes were genotyped to analyze the association between these tSNPs and susceptibility to HIV-1 infection and AIDS progression among MSM (529 HIV − 1 seropositive men and 529 HIV-1 seronegative men) in northern China. The results showed that rs34660854-A and rs75368165-A in ATR gene and rs3756766-A in Cdc25C gene could increase the risk of HIV-1 infection (P = 0.049, P = 0.020 and P = 0.010, respectively), and ATR rs75069062 and Chk1 rs10893405 were significantly associated with AIDS progression (P = 0.026 and P = 0.029, respectively). Besides, rs34660854 and rs75368165 in ATR gene, rs12576279 and rs540436 in Chk1 gene, rs3756766 in Cdc25C gene and rs139245206 in CDK1 gene were significantly associated with HIV-1 infection under different models (P
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- 2020
60. Combinatorial approach of in silico and in vitro evaluation of MLH1 variant associated with Lynch syndrome like metastatic colorectal cancer
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Shuhan Si, Jie Wu, Siqi Zhu, Songbin Fu, Kexian Dong, Qian Qin, Wenjing Sun, Wei Ji, Xueyuan Jia, Chunhui Zhang, Tahir Zaib, Yusi Wang, Lidan Xu, Hanfei Yu, and Komal Saleem
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0301 basic medicine ,Sanger sequencing ,In silico ,Biophysics ,Microsatellite instability ,Cell Biology ,Biology ,medicine.disease ,MLH1 ,Biochemistry ,digestive system diseases ,Lynch syndrome ,03 medical and health sciences ,symbols.namesake ,030104 developmental biology ,0302 clinical medicine ,Germline mutation ,030220 oncology & carcinogenesis ,medicine ,symbols ,Cancer research ,DNA mismatch repair ,Molecular Biology ,Exome sequencing - Abstract
Colorectal cancer (CRC) is the third most developing cancer worldwide and Lynch syndrome (LS) accounts for 3–4% of CRC. Genetic alteration in any of DNA mismatch repair (MMR) gene is the major cause of LS that disrupt the normal upstream and downstream MMR events. Germline mutation of MLH1 in heterozygous state have an increased risk for CRC. Defective MMR pathway mostly results in microsatellite instability (MSI) that occurs in high percentage of CRC associated tumors. Here, we reported a patient with LS like metastatic CRC (mCRC) associated with other related cancers. Whole exome sequencing (WES) of the proband was performed to identify potential causative gene. Genetic screening validated by Sanger sequencing identified a heterozygous missense mutation in exon 12 of MLH1 (c.1151T>A, p.V384D). The clinical significance of identified variant was elucidated on the basis of clinicopathological data, computational predictions and various in vitro functional analysis. In silico predictions classified the variant to be deleterious and evolutionary conserved. In vitro functional studies revealed a significant decrease in protein expression because of stability defect leading to loss of MMR activity. Mutant residue found in MutL transducer domain of MLH1 that localized in the nucleus but translocation was not found to be significantly disturbed. In conclusion, our study give insight into reliability of combinatorial prediction approach of in silico and in vitro expression analysis. Hence, we highlighted the pathogenic correlation of MLH1 variant with LS associated CRC as well as help in earlier diagnosis and surveillance for improved management and genetic counselling.
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- 2020
61. SNPs in folate pathway are associated with the risk of nonsyndromic cleft lip with or without cleft palate, a meta-analysis
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Tahir Zaib, Xueyuan Jia, Lidan Xu, Wenjing Sun, Qiuyan Li, Komal Saleem, and Songbin Fu
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0301 basic medicine ,Oncology ,Folate ,MTRR ,Biochemistry ,0302 clinical medicine ,Gene Frequency ,Risk Factors ,Genotype ,Medicine ,Research Articles ,biology ,Brain ,Genomics ,Cleft Palate ,Ferredoxin-NADP Reductase ,Muscle Spasticity ,Meta-analysis ,Homocystinuria ,China ,medicine.medical_specialty ,Bioinformatics ,Cleft Lip ,Biophysics ,SNP ,Single-nucleotide polymorphism ,Subgroup analysis ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,Folic Acid ,Asian People ,Internal medicine ,Genetic model ,Humans ,Genetic Predisposition to Disease ,Molecular Biology ,Methylenetetrahydrofolate Reductase (NADPH2) ,business.industry ,030206 dentistry ,Cell Biology ,Publication bias ,030104 developmental biology ,Psychotic Disorders ,Case-Control Studies ,Methylenetetrahydrofolate reductase ,Mutation ,MTHFR ,biology.protein ,business - Abstract
Background: Prenatal intake of folic acid is important for prevention of NSCL/P (nonsyndromic cleft lip with or without cleft palate). Associated genes in folate pathway are major enzymes of folic acid metabolism that is crucial for preventing birth defects. The present meta-analysis aims to investigate the association between four SNPs in folate pathway genes and the risk of NSCL/P. Methods: Comprehensive bioinformatics analysis was used to predict the functional pathogenicity of genetic variation. The PubMed, Embase database and Google Scholar were searched by two researchers. Stata 11.0 software was used to analyze the results. Subgroup analysis was carried out to assess the influence of genetic background. Sensitivity analysis, regression analysis and publication analysis were also conducted to enhance the strength of our results. Results: It is estimated that the probability of two missense mutation rs1801133 in MTHFR and rs1801394 in MTRR are more likely to be damaging by bioinformatics analysis. A significant association between rs1801133 and risk of NSCL/P in two genetic models: TT genotype vs CC genotype (OR = 1.333 95%CI = 1.062–1.674, P = 0.013), and recessive model (OR = 1.325 95%CI = 1.075–1.634, P = 0.008). A significant protective association between rs1801394 GG genotype and NSCL/P in Asian (GG vs AA, OR = 0.520 95%CI = 0.321–0.841, P = 0.008) was observed. Meta-regression, sensitivity analysis, and publication bias analysis confirmed that the results of the present study were statistically significant. Conclusions: The present study identified that rs1801133 in MTHFR is associated with the risk of NSCL/P, and rs1801394 GG genotype in MTRR play a protective role in Asian. Further, larger studies should be performed to confirm these findings.
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- 2020
62. Identification of key genes associated with multiple sclerosis based on gene expression data from peripheral blood mononuclear cells
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Mingming Zhang, Ruijie Zhang, Zhenwei Shang, Xueyuan Jia, Songbin Fu, Lidan Xu, and Wenjing Sun
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Candidate gene ,Microarray ,Interaction ,Bioinformatics ,Data Mining and Machine Learning ,SNP ,lcsh:Medicine ,Single-nucleotide polymorphism ,Computational biology ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Multiple sclerosis ,03 medical and health sciences ,0302 clinical medicine ,Enrichment anclysis ,microRNA ,Gene expression ,medicine ,Gene ,030304 developmental biology ,miRNA ,0303 health sciences ,General Neuroscience ,Statistics ,lcsh:R ,General Medicine ,Protein protein interaction networks ,medicine.disease ,General Agricultural and Biological Sciences ,030217 neurology & neurosurgery - Abstract
The aim of this study was to identify the potential key candidate genes of multiple sclerosis (MS) and uncover mechanisms in MS. We combined data from the microarray expression profile of three MS stages and performed bioinformatics analysis. Differentially expressed genes (DEGs) were identified among the distinct stages of MS and healthy controls, and a total of 349 shared DEGs were identified. Gene ontology (GO) and pathway enrichment analyses showed that the DEGs were significantly enriched in the biological processes (BPs) of purine-related metabolic processes and signaling, especially the common DEGs, which were enriched in some immunological processes. Most of the DEGs were enriched in signaling pathways associated with the immune system, some immune diseases and infectious disease pathways. Through a protein–protein interaction (PPI) network analysis and a gene expression regulatory network constructed with MS-related miRNAs, we confirmed FOS, TP53, VEGFA, JUN, HIF1A, RB1, PTGS2, CXCL8, OAS2, NFKBIA and OAS1 as candidate genes of MS. Furthermore , we explored the potential SNPs associated with MS by database mining. In conclusion, this study provides the identified genes, SNPs, biological processes, and cellular pathways associated with MS. The uncovered candidate genes may be potential biomarkers involved in the diagnosis and therapy of MS.
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- 2020
63. Functional Characterization of a Missense Variant of MLH1 Identified in Lynch Syndrome Pedigree
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Songbin Fu, Wei Ji, Lidan Xu, Chunhui Zhang, Hanfei Yu, Jie Wu, Wei Gao, Hanif Khan, Siqi Zhu, Yanqiao Zhang, Hongtao Song, Wenjing Sun, Yun Huang, Xueyuan Jia, Komal Saleem, Yusi Wang, Tahir Zaib, and Qian Qin
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0301 basic medicine ,Adult ,Male ,Medicine (General) ,medicine.medical_specialty ,China ,congenital, hereditary, and neonatal diseases and abnormalities ,Genetic counseling ,Clinical Biochemistry ,Mutation, Missense ,Down-Regulation ,Biology ,MLH1 ,03 medical and health sciences ,symbols.namesake ,R5-920 ,0302 clinical medicine ,Genotype ,Exome Sequencing ,Genetics ,medicine ,PMS2 ,Missense mutation ,Humans ,Genetic Predisposition to Disease ,Molecular Biology ,Genetic Association Studies ,Mismatch Repair Endonuclease PMS2 ,Sanger sequencing ,Biochemistry (medical) ,High-Throughput Nucleotide Sequencing ,General Medicine ,Middle Aged ,medicine.disease ,Colorectal Neoplasms, Hereditary Nonpolyposis ,Lynch syndrome ,digestive system diseases ,Pedigree ,030104 developmental biology ,030220 oncology & carcinogenesis ,symbols ,Medical genetics ,Female ,MutL Protein Homolog 1 ,Research Article - Abstract
Lynch syndrome (LS) is the most common hereditary colorectal cancer (CRCs) inherited in an autosomal-dominant manner. Here, we reported a multigeneration Chinese family clinically diagnosed with LS according to the Amsterdam II criteria. To identify the underlying causative gene for LS in this family, whole-exome sequencing (WES) was performed. A germline missense variant (c.2054C>T:p.S685F) in exon 18 of MLH1 was successfully identified by WES. Sanger sequencing verified the results of WES and also confirmed the cosegregation of the MLH1 missense variant in all affected members of the family including two unaffected family members. Bioinformatic tools predicted the identified MLH1 variant as deleterious. Immunohistochemistry (IHC) staining showed loss of MLH1 and PMS2 protein expression. In vitro expression analysis also revealed that the identified MLH1 missense variant (c.2054C>T:p.S685F) results in reduced expression of both MLH1 and PMS2 proteins. Based on the American College of Medical Genetics and Genomics (ACMG) guidelines, the missense mutation c.2054C>T in MLH1 was classified as a “pathogenic” variant. Two unaffected family members were later recommended for colonoscopy and other important cancer diagnostic inspections every 1-2 years as both were at higher risk of LS. In conclusion, our findings widen the genotypic spectrum of MLH1 mutations responsible for LS. This study increases the phenotypic spectrum of LS which will certainly help the clinicians in diagnosing LS in multigeneration families. This study also puts emphasis on the importance of genetic counselling for the benefit of asymptomatic carriers of MMR gene variants who are at higher risk of LS.
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- 2020
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64. Association of single nucleotide polymorphisms in TCF2 with type 2 diabetes susceptibility in a Han Chinese population.
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Xuelong Zhang, Hong Qiao, Yanling Zhao, Xi Wang, Haiming Sun, An Liu, Lidan Xu, Donglin Sun, Yan Jin, Yang Yu, Xiangning Meng, Jing Bai, Feng Chen, and Songbin Fu
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Medicine ,Science - Abstract
Hepatocyte nuclear factor 1β (HNF1β), a transcription factor encoded by the transcription factor 2 gene (TCF2), plays a critical role in pancreatic cell formation and glucose homeostasis. It has been suggested that single nucleotide polymorphisms (SNPs) of TCF2 are associated with susceptibility to type 2 diabetes (T2D). However, published results are inconsistent and inclusive. To further investigate the role of these common variants, we examined the association of TCF2 polymorphisms with the risk of T2D in a Han population in northeastern China. We genotyped five SNPs in 624 T2D patients and 630 healthy controls by using a SNaPshot method, and evaluated the T2D risk conferred by individual SNPs and haplotypes. In the single-locus analysis, we found that rs752010, rs4430796 and rs7501939 showed allelic differences between T2D patients and healthy controls, with an OR of 1.26 (95% CI 1.08-1.51, P = 0.003), an OR of 1.23 (95% CI 1.06-1.55, P = 0.001) and an OR of 1.28 (95% CI 1.10-1.61, P = 0.001), respectively. Genotype association analysis of each locus also revealed that the homozygous carriers of the at-risk allele had a significant increased T2D risk compared to homozygous carriers of the other allele (OR 1.78, 95% CI 1.20-2.64 for rs752010; OR 1.82, 95% CI 1.24-2.67 for rs4430796; OR 1.95, 95% CI 1.31-2.90 for rs7501939), even after Bonferroni correction for multiple comparisons. Besides, the haplotype-based analysis demonstrated that AGT in block rs752010-rs4430796-rs7501939 was associated with about 30% increase in T2D risk (OR 1.31, 95% CI 1.09-1.57, P = 0.01). Our findings suggested that TCF2 variants may be involved in T2D risk in a Han population of northeastern China. Larger studies with ethnically diverse populations are warranted to confirm the results reported in this investigation.
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- 2012
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65. In situ monitoring of cytoplasmic precursor and mature microRNA using gold nanoparticle and graphene oxide composite probes
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Meifang Li, Lidan Xu, Bo Tang, Min Hong, Qiaoli Yue, Chen-Zhong Li, Hongxiao Sun, and Guodong Shen
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0301 basic medicine ,In situ ,Fluorescence-lifetime imaging microscopy ,Metal Nanoparticles ,Nanoparticle ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Cell Line ,Analytical Chemistry ,03 medical and health sciences ,microRNA ,Humans ,Environmental Chemistry ,Spectroscopy ,Detection limit ,Chemistry ,Oxides ,0104 chemical sciences ,MicroRNAs ,030104 developmental biology ,Cytoplasm ,Molecular Probes ,Cancer cell ,Nucleic acid ,Biophysics ,Graphite ,Gold - Abstract
This study strategically fabricates a nucleic acid functionalized gold nanoparticle and graphene oxide composite probe (AuNP/GO probe) to achieve both the recognition and in situ monitoring of cytoplasmic target precursor microRNAs (pre-miRNAs) and mature microRNAs (miRNAs) in living cells. The pre-miRNA-21 detection with AuNP probes has a good linear range of 0–300 nM and a limit of detection (LOD) of 4.5 nM, whereas the GO probe has a linear relationship with mature miRNA-21 from 0.1 to 10 nM with a LOD of 1.74 nM. This assay was utilized to directly visualize the relative expression levels of pre- and mature forms of miRNA-21 and let-7a. The results suggested that the expression levels of precursor miRNAs remain constant in cancer cells and normal cells. However, the expression levels of mature miRNAs vary widely, demonstrating the “up-regulation” of miRNA-21 and “down-regulation” of let-7a in cancer cells in contrast to that in normal cells. The practicality of this strategy was verified by in situ monitoring changes in cytoplasmic pre-miRNA-21 and mature miRNA-21 in response to small-molecule inhibitors of miRNA-21.
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- 2018
66. Two SNPs rs1801133 and rs1801394 in folate pathway are associated with the risk of nonsyndromic cleft lip with or without cleft palate
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Lidan Xu, Qiuyan Li, Songbin Fu, Tahir Zaib, Wenjing Sun, Komal Saleem, and Xueyuan Jia
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business.industry ,Medicine ,Single-nucleotide polymorphism ,business ,Bioinformatics - Abstract
Background Prenatal intake of folic acid is important for prevention of nonsyndromic cleft lip with or without cleft palate (NSCL/P). Associated genes in folate pathway are major enzymes of folic acid metabolism that is crucial for preventing birth defects. The present study aims to investigate the association between four single nucleotide polymorphisms (SNPs) in folate pathway genes and the risk of NSCL/P. Methods Comprehensive bioinformatics analysis was used to predict the functional pathogenicity of genetic variation. The PubMed, Embase database and Google Scholar were intensively searched by two researchers to ascertain all relevant studies. Stata 11.0 software was used to analyze the results. Subgroup analysis was carried out to assess the influence of genetic background. Sensitivity analysis, regression analysis and publication analysis were also conducted to enhance the strength of our results. Results It is estimated that the probability of two missense mutation rs1801133 in MTHFR and rs1801394 in MTRR are more likely to be damaging by bioinformatics analysis. A total of 34 publications were included in the present study analysis. Our results showed a significant association between rs1801133 and risk of NSCL/P in two genetic models: TT allele vs CC allele (OR=1.333 95%CI=1.062-1.674, P =0.013), and recessive model (OR=1.325 95%CI=1.075-1.634, P =0.008). A significant association between rs1801394 and the risk of NSCL/P in Asian (GG allele vs AA allele, OR=0.520 95%CI=0.321-0.841, P =0.008) was also observed. Meta-regression, sensitivity analysis, and publication bias analysis confirmed that the results of the present study were statistically significant. Conclusions The present study highlights two SNPs rs1801133 in MTHFR and rs1801394 in MTRR, associated with the increasing risk of NSCL/P. Further, larger studies should be performed to confirm these findings.
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- 2019
67. Association between the SNPs of the TOB1 gene and gastric cancer risk in the Chinese Han population of northeast China
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Hui Wang, Huiting Hao, Xuelong Zhang, Haonan Guo, Lidan Xu, Jingcui Yu, and Yuanyuan Wang
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0301 basic medicine ,medicine.medical_specialty ,Population ,Chromosomal translocation ,Single-nucleotide polymorphism ,genetic risk ,Gastroenterology ,TOB-1 ,03 medical and health sciences ,0302 clinical medicine ,Chinese han population ,single nucleotide polymorphism ,Internal medicine ,Genotype ,medicine ,education ,Gene ,education.field_of_study ,business.industry ,gastric cancer ,Haplotype ,Cancer ,medicine.disease ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,business ,Research Paper - Abstract
The TOB1 (ErbB-2,1) gene is an anti-proliferative factor that has the potential to regulate cell growth and encodes a member of the transducer of erbB-2/B-cell translocation gene protein. The association between the polymorphisms of the TOB1 gene and gastric cancer (GC) risk is still unclear. In this study, 506 GC cases and 548 healthy controls (HCs) were collected to evaluate the association between the eleven SNPs (rs35220381, rs12950561, rs7221352, rs61482741, rs9303568, rs34700818, rs12949115, rs9903822, rs12601477, rs11656976 and rs4626) of the TOB1 gene and GC risk in the population of northeast China. The results showed that there were significant associations of haplotype GCCTTGC, haplotype ATCTTGG, and haplotype GCCACGC with GC risk (P < 0.05, P < 0.001, and P
- Published
- 2018
68. The distribution of three candidate cold-resistant SNPs in six minorities in North China
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Xuelong Zhang, Wenjing Sun, Jie Wu, Qiuyan Li, Lidan Xu, Kexian Dong, Xueyuan Jia, and Songbin Fu
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0301 basic medicine ,Fatty Acid Desaturases ,Linkage disequilibrium ,China ,Genotype ,lcsh:QH426-470 ,FADS2 ,lcsh:Biotechnology ,Zoology ,Distribution (economics) ,SNP ,Single-nucleotide polymorphism ,Biology ,Polymorphism, Single Nucleotide ,CPT1A ,Linkage Disequilibrium ,03 medical and health sciences ,North China ,Asian People ,Gene Frequency ,lcsh:TP248.13-248.65 ,Genetics ,Ethnicity ,Humans ,Allele frequency ,Phylogeny ,Minorities ,chemistry.chemical_classification ,FADS ,Carnitine O-Palmitoyltransferase ,business.industry ,Adaptation, Physiological ,Cold Temperature ,lcsh:Genetics ,030104 developmental biology ,chemistry ,business ,Biotechnology ,Polyunsaturated fatty acid ,Research Article - Abstract
Background Heilongjiang Province located in northeast China is a multi-ethnic region with people who have lived in cold conditions for several generations. Fatty acids are important to people with cold resistance. CPT1A encodes a protein that imports long-chain fatty acids into the mitochondria for fatty-acid oxidation. FADS is an essential enzyme for the synthesis of long-chain polyunsaturated fatty acids. Results In the present study, we investigated the distributions of three cold resistance-related SNPs (rs80356779 G > A in CPT1A, rs7115739 T > G in FADS3 and rs174570 C > T in FADS2) from six populations that included 1093 individuals who have lived in Heilongjiang Province for at least three generations. The frequencies of rs174570 and rs7115739 were different in our six north minorities compared to the Chinese Dai in Xishuangbanna (CDX) in southern China. All the SNPs in Hezhen were significantly different from other five studied populations. In addition, the genetic distribution of rs174570 in Daur was significantly different from Manchu and Korea, and the frequency of rs7115739 in Ewenki was significantly different from the other populations. The results also showed that the frequencies of the three SNPs in the six minorities were different from those of Greenlandic Inuit and Siberian population. Conclusions Our results showed the distributions of the three cold resistance-related SNPs from six populations that included 1093 individuals in northern China. Distributions of the allele frequencies for the cold resistance-related SNPs in northern China were statistically different from those in southern China. These data help to establish the DNA genome database for the six populations and fully preserve existing minority genetic information. Electronic supplementary material The online version of this article (10.1186/s12864-018-4524-1) contains supplementary material, which is available to authorized users.
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- 2018
69. Receptivity of the Boundary Layer over a Blunt Wedge with Distributed Roughness at Mach 6
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Mingfang Shi, Lidan Xu, Xiaojun Tang, Xiaokun Sun, and Zhenqing Wang
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020301 aerospace & aeronautics ,Hypersonic speed ,Materials science ,Article Subject ,lcsh:Motor vehicles. Aeronautics. Astronautics ,Direct numerical simulation ,Aerospace Engineering ,02 engineering and technology ,Acoustic wave ,Mechanics ,Surface finish ,01 natural sciences ,Wedge (geometry) ,010305 fluids & plasmas ,Vortex ,Boundary layer ,symbols.namesake ,0203 mechanical engineering ,Mach number ,0103 physical sciences ,symbols ,lcsh:TL1-4050 - Abstract
A hypersonic flow field over a blunt wedge with or without roughness is simulated by a direct numerical simulation method. The effect of isolated and distributed roughnesses on the steady and unsteady hypersonic flow field and boundary layer is analyzed. The shape of roughness is controlled by cubic polynomial. The evolution of disturbance waves caused by slow acoustic wave in the boundary layer is investigated by fast Fourier spectrum analysis. The results show that there is a great influence of roughness on the evolution of disturbance waves in the hypersonic boundary layer. The disturbance waves are promoted in the upstream-half region of roughness while suppressed in the downstream-half region of roughness. There is always a mode competition among different modes both in the temporal domain and in the frequency domain in the boundary layer, and mode competition is affected by roughness. The location of the dominant mode which is changed to a second-order harmonic mode from the fundamental mode moves upstream. The vortices caused by roughness also impact the evolution of disturbance waves in the boundary layer. The fundamental mode is suppressed in the vortex region while other harmonic modes are promoted.
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- 2018
70. COX-2 expression in ovarian cancer: an updated meta-analysis
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Xuelong Zhang, Yan Jin, Lidan Xu, Donglin Sun, Haiming Sun, Yuandong Qiao, and Xueyuan Jia
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,Pathology ,Subgroup analysis ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,Internal medicine ,medicine ,Stage (cooking) ,Survival rate ,business.industry ,Hazard ratio ,COX-2 ,medicine.disease ,Confidence interval ,meta-analysis ,ovarian cancer ,030104 developmental biology ,030220 oncology & carcinogenesis ,Meta-analysis ,prognosis ,Ovarian cancer ,business ,Research Paper - Abstract
The prognostic role of COX-2 expression in ovarian cancer patients has been studied for years, while results remain controversial. Thus we performed a meta-analysis to evaluate the prognostic impact of COX-2 expression on survival of ovarian cancer patients. The databases PubMed, Embase and CNKI were searched. Summary hazard ratio (HR) and 95% confidence intervals (CIs) were calculated to analyze the correlations between COX-2 expression and overall survival (OS), and disease-free survival (DFS). A total of 1,867 patients from 18 studies were enrolled in the final analysis. The results showed that patients with higher COX-2 expression had a poor OS (HR: 1.48; 95% CI: 1.19-1.85) and DFS (HR: 1.81, 95% CI: 1.28-2.55). Subgroup analysis showed that there had significant associations between COX-2 expression and survival rate in most of the subgroups. Furthermore, there were significant associations between COX-2 expression and several clinical parameters such as FIGO stage, histological type and age. These results showed the patients with higher COX-2 expression had a significantly poorer survival rate, COX-2 expression had the potential to be a prognostic marker of ovarian cancer.
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- 2017
71. Fatigue behavior of glass-fiber-reinforced epoxy composites embedded with shape memory alloy wires
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Zhenqing Wang, Mingfang Shi, Lidan Xu, Xiaoyu Sun, and Jingbiao Liu
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Materials science ,Scanning electron microscope ,Glass fiber ,02 engineering and technology ,Shape-memory alloy ,Epoxy ,Composite laminates ,021001 nanoscience & nanotechnology ,SMA ,Residual strength ,020303 mechanical engineering & transports ,0203 mechanical engineering ,visual_art ,Ultimate tensile strength ,Ceramics and Composites ,visual_art.visual_art_medium ,Composite material ,0210 nano-technology ,Civil and Structural Engineering - Abstract
The tensile fatigue behavior of glass-fiber-reinforced epoxy (GF/epoxy) composites embedded with shape memory alloy (SMA) wires prepared by vacuum assisted resin infusion (VARI) processing was investigated. The experiments were performed under cyclic tensile load. The damage mechanism of SMA composites under different stress levels of cyclic loadings was revealed. The fracture surface and fatigue failure modes of the specimens were examined and analyzed according to the damage mechanism under different stress levels. The stress-number (S-N) curves, the residual strength and residual stiffness of specimens were calculated after the experiment. The results show that the fatigue life of SMA composites is more than twice higher than GF/epoxy composites. Microstructural analysis using the scanning electron microscopy (SEM) technique was conducted on fractured surfaces to fully understand the failure mechanism. The fatigue failure modes in SMA composite laminates were revealed.
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- 2017
72. Numerical analysis of mechanical performances of shape memory alloy hybrid composites
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Ming-Fang Shi, Hong Wang, Jitao Zhao, Zhenqing Wang, Lidan Xu, Gang Xue, Xiangyu Zhang, and Xiaoyu Sun
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Materials science ,Numerical analysis ,Composite number ,Glass fiber ,02 engineering and technology ,Shape-memory alloy ,Epoxy ,Composite laminates ,010402 general chemistry ,021001 nanoscience & nanotechnology ,SMA ,01 natural sciences ,Finite element method ,0104 chemical sciences ,Mechanics of Materials ,visual_art ,Materials Chemistry ,visual_art.visual_art_medium ,General Materials Science ,Composite material ,0210 nano-technology - Abstract
The effects of the content and position of SMA wires on the static properties of glass fiber reinforced epoxy (GF/epoxy) composite laminates prepared by vacuum-assisted resin infusion (VARI) process were investigated. According to the influence of different embedding content and position of SMA wire on mechanical performances of composite laminates, the optimal content and optimal position of SMA were found out. The static mechanical finite element models of GF/epoxy composite and shape memory alloy hybrid composites (SMAHCs) are established. The validity of the models is verified by comparing the simulation results with the experimental results. According to the finite element results, the influence of SMA embedding content and position on the mechanical performances of composites is described quantitatively.
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- 2021
73. Experimental and numerical investigation on the low-velocity impact response of shape memory alloy hybrid composites
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Xiangyu Zhang, Zhenqing Wang, Gang Xue, Mingfang Shi, and Lidan Xu
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Materials science ,Computer simulation ,Composite number ,02 engineering and technology ,Epoxy ,Shape-memory alloy ,010402 general chemistry ,021001 nanoscience & nanotechnology ,SMA ,Threshold energy ,01 natural sciences ,0104 chemical sciences ,Mechanics of Materials ,Energy absorption ,visual_art ,Materials Chemistry ,visual_art.visual_art_medium ,General Materials Science ,Composite material ,0210 nano-technology ,Energy (signal processing) - Abstract
The impact response of shape memory alloy hybrid composite (SMAHC) prepared by vacuum-assisted resin infusion was investigated experimentally and numerically. The impact properties of shape memory alloy composites under three impact stages were characterized. A numerical simulation method is used to simulate the impact damage evolution process of SMAHCs. The experimental results are consistent with the numerical results, which further verified the effectiveness of the laminate against impact load after embedding SMA. The impact threshold energy value of impact damage of SMAHCs was found by numerical simulation. The results show that the impact failure of SMAHC laminates requires more energy than GF/epoxy composites due to the excellent energy absorption capacity of SMA.
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- 2021
74. So Close I Can Almost Sense It: The Interplay between Sensory Imagery and Psychological Distance
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Morgan Poor, Ann E. Schlosser, Lidan Xu, and Ryan S. Elder
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Marketing ,Economics and Econometrics ,Persuasion ,Communication ,business.industry ,media_common.quotation_subject ,05 social sciences ,Sensory system ,Stimulus (physiology) ,050105 experimental psychology ,Sight ,Stimulus modality ,Arts and Humanities (miscellaneous) ,Anthropology ,Perception ,0502 economics and business ,050211 marketing ,0501 psychology and cognitive sciences ,Construal level theory ,Business and International Management ,Psychology ,business ,media_common ,Cognitive psychology - Abstract
Across the five sensory modalities we examine an unexplored difference in imagery: psychological distance. In particular, we propose that imagined senses can be psychologically more proximal or distal based on the maximum physical distance typically required for a stimulus to be sensed. Specifically, we propose that imagined senses that require close proximity to the body in order to be sensed (i.e., taste, touch) will feel more psychologically proximal than senses that do not require such close proximity (i.e., hearing, sight). We obtain support for our theoretical framework across a pilot study, four lab studies, and one field study by examining how images evoked using different sensory modalities differentially influence variables shown in past research to vary along psychological distance: (1) the imagined distance between the consumer and the stimulus, (2) product perceptions on other dimensions of psychological distance, and (3) persuasion when matched with other dimensions of psychological distance.
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- 2017
75. Association between the BRCA2 rs144848 polymorphism and cancer susceptibility: a meta-analysis
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Chang Liu, Ning He, Haiming Sun, Rongwei Guan, Jingcui Yu, Lidan Xu, Qiuyan Li, Yuandong Qiao, Xueyuan Jia, and Xuelong Zhang
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,Population ,Subgroup analysis ,Review ,susceptibility ,polymorphism ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Risk Factors ,Neoplasms ,Internal medicine ,Genetic model ,medicine ,cancer ,Humans ,Genetic Predisposition to Disease ,education ,BRCA2 Protein ,education.field_of_study ,Polymorphism, Genetic ,business.industry ,Case-control study ,Publication bias ,Prognosis ,medicine.disease ,BRCA2 ,meta-analysis ,030104 developmental biology ,Systematic review ,Case-Control Studies ,030220 oncology & carcinogenesis ,Meta-analysis ,business - Abstract
The BRCA2 gene plays an important role in cancer carcinogenesis, and polymorphisms in this gene have been associated with cancer risk. The BRCA2 rs144848 polymorphism has been associated with several cancers, but results have been inconsistent. In the present study, a meta-analysis was performed to assess the association between the rs144848 polymorphism and cancer risk. Literature was searched from the databases of PubMed, Embase and Google Scholar before April 2016. The fixed or random effects model was used to calculate pooled odd ratios on the basis of heterogeneity. Meta-regression, sensitivity analysis, subgroup analysis and publication bias assessment were also performed using STATA 11.0 software according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2009. A total of 40 relevant studies from 30 publications including 34,911 cases and 48,329 controls were included in the final meta-analysis. Among them, 22 studies focused on breast cancer, seven on ovarian cancer, five on non-Hodgkin lymphoma, and the remaining six studies examined various other cancers. The meta-analysis results showed that there were significant associations between the rs144848 polymorphism and cancer risk in all genetic models. Stratified by cancer type, the rs144848 polymorphism was associated with non-Hodgkin lymphoma. Stratified by study design, the allele model was associated with breast cancer risk in population-based studies. The meta-analysis suggests that the BRCA2 rs144848 polymorphism may play a role in cancer risk. Further well-designed studies are warranted to confirm these results.
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- 2017
76. Fried-food consumption and risk of cardiovascular disease and all-cause mortality: a meta-analysis of observational studies.
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Pei Qin, Ming Zhang, Minghui Han, Dechen Liu, Xinping Luo, Lidan Xu, Yunhong Zeng, Qing Chen, Tieqiang Wang, Xiaoliang Chen, Qionggui Zhou, Quanman Li, Ranran Qie, Xiaoyan Wu, Dongsheng Hu, Fulan Hu, Qin, Pei, Zhang, Ming, Han, Minghui, and Liu, Dechen
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CARDIOVASCULAR disease related mortality ,CARDIOVASCULAR diseases risk factors ,CARDIOVASCULAR diseases ,HEART failure ,CORONARY disease ,MORTALITY ,CAUSES of death ,RESEARCH ,CONVENIENCE foods ,META-analysis ,RESEARCH methodology ,DISEASE incidence ,WORLD health ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies - Abstract
Objective: We performed a meta-analysis, including dose-response analysis, to quantitatively determine the association of fried-food consumption and risk of cardiovascular disease and all-cause mortality in the general adult population.Methods: We searched PubMed, EMBASE and Web of Science for all articles before 11 April 2020. Random-effects models were used to estimate the summary relative risks (RRs) and 95% CIs.Results: In comparing the highest with lowest fried-food intake, summary RRs (95% CIs) were 1.28 (1.15 to 1.43; n=17, I2=82.0%) for major cardiovascular events (prospective: 1.24 (1.12 to 1.38), n=13, I2=75.7%; case-control: 1.91 (1.15 to 3.17), n=4, I2=92.1%); 1.22 (1.07 to 1.40; n=11, I2=77.9%) for coronary heart disease (prospective: 1.16 (1.05 to 1.29), n=8, I2=44.6%; case-control: 1.91 (1.05 to 3.47), n=3, I2=93.9%); 1.37 (0.97 to 1.94; n=4, I2=80.7%) for stroke (cohort: 1.21 (0.87 to 1.69), n=3, I2=77.3%; case-control: 2.01 (1.27 to 3.19), n=1); 1.37 (1.07 to 1.75; n=4, I2=80.0%) for heart failure; 1.02 (0.93 to 1.14; n=3, I2=27.3%) for cardiovascular mortality; and 1.03 (95% CI 0.96 to 1.12; n=6, I2=38.0%) for all-cause mortality. The association was linear for major cardiovascular events, coronary heart disease and heart failure.Conclusions: Fried-food consumption may increase the risk of cardiovascular disease and presents a linear dose-response relation. However, the high heterogeneity and potential recall and misclassification biases for fried-food consumption from the original studies should be considered. [ABSTRACT FROM AUTHOR]- Published
- 2021
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77. Fluorescence Imaging of Intracellular Telomerase Activity Using Enzyme-Free Signal Amplification
- Author
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Min Hong, Lidan Xu, Qingwang Xue, Bo Tang, and Lu Li
- Subjects
Fluorescence-lifetime imaging microscopy ,Telomerase ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,Humans ,Sulfhydryl Compounds ,Repeated sequence ,Enzyme Assays ,Chemistry ,Optical Imaging ,Nucleic acid sequence ,Oxides ,DNA ,021001 nanoscience & nanotechnology ,Fluorescence ,0104 chemical sciences ,Biochemistry ,Colloidal gold ,Nucleic acid ,Nanoparticles ,Graphite ,Gold ,Primer (molecular biology) ,0210 nano-technology ,HeLa Cells - Abstract
A novel enzyme-free signal amplification-based assay for highly sensitive in situ fluorescence imaging and detection of intracellular telomerase activity was developed by using a gold nanoflare probe-triggered mimic-hybridization chain reaction (mimic-HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. The nanoflare probe consists of gold nanoparticles (AuNPs) functionalized with a dense shell of nucleic acid sequences by Au-S bond formation. The nucleic acid sequence is composed of three segments: a long thiol-labeled sequence (HS-DNA) and two short sequences (a telomerase primer sequence, "Primer-DNA", and an FAM-terminated reporter sequence, "Flare-DNA"), both of which are complementary to HS-DNA. The mimic-HCR system is formed by two FAM-modified hairpin sequences that are adsorbed on GO. Upon endocytosis of the AuNP/GO combinatorial probe, the Primer-DNA can be extended by intracellular telomerase at its 3' end to produce the telomeric repeated sequence, which leads to inner chain substitution and not only releases the Flare-DNA to turn on the fluorescence of FAM but also initiates the subsequent signal amplification and enrichment for the mimic-HCR system anchored on GO. The proposed approach can sensitively detect telomerase activity in living cells, distinguish normal cells from cancer cells, and monitor the change in telomerase activity in response to a telomerase inhibitor.
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- 2016
78. Two SNPs rs1801133 and rs1801394 in folate pathway are associated with the risk of nonsyndromic cleft lip with or without cleft palate
- Author
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Qiuyan Li, Lidan Xu, Xueyuan Jia, Tahir Zaib, Wenjing Sun, and Songbin Fu
- Abstract
Background Prenatal intake of folic acid is important for prevention of nonsyndromic cleft lip with or without cleft palate (NSCL/P), genes participant in folate pathway are crucial for preventing birth defects. The present study aims to investigate the associations between four single nucleotide polymorphisms (SNPs) in folate pathway genes and the risk of NSCL/P. Methods Prediction by bioinformatics was conducted to assess the function of genetic variation. The PubMed, Embase database and Google Scholar were searched by two researchers to identify all relevant studies. Stata 11.0 software was used to calculate the results. Subgroup analysis was conducted to assess the influence of the genetic background. Sensitivity analysis, regression analysis and publication analysis were conducted to improve the strength of the results. Results Two genetic variations rs1801394 in MTRR gene and rs1801133 in MTHFR were predicted damaging. A total of 34 publications were included in the present analysis. The results showed that there were a significant association between rs1801133 and NSCL/P risk in two genetic models TT allele vs CC allele (OR=1.333 95%CI=1.062-1.674, P=0.013) and recessive model (OR=1.325 95%CI=1.075-1.634, P=0.008) and there were a significant association between rs1801394 and NSCL/P risk in Asian (GG allele vs AA allele, OR=0.520 95%CI=0.321-0.841, P=0.008). Meta-regression, sensitivity analysis, and publication bias analysis confirmed that the results of the present study were statistically robust. Conclusions This present study suggests that rs1801133 of the MTHFR gene and rs1801394 of the MTRR gene are risk factors for NSCL/P. Additional, larger studies should be performed to confirm these findings.
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- 2019
79. The Association Between
- Author
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Zhaohui, Xing, Guiying, Guo, Xinling, Pan, Lidan, Xu, Chaopu, Guo, and Ruihua, An
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Male ,China ,MutS Homolog 2 Protein ,Genotype ,Humans ,Female ,Middle Aged ,MutL Protein Homolog 1 ,DNA Mismatch Repair ,Polymorphism, Single Nucleotide ,Genetic Association Studies ,Kidney Neoplasms ,Retrospective Studies - Published
- 2019
80. The association of TNF-α -308G/A and -238G/A polymorphisms with type 2 diabetes mellitus: a meta-analysis
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Chang Liu, Xiaoliang Guo, Chenxi Li, Qingbu Mei, Lidan Xu, Wenjing Sun, Jiawei Wu, and Songbin Fu
- Subjects
0301 basic medicine ,Oncology ,238G/A ,medicine.medical_specialty ,Candidate gene ,Genotype ,Biophysics ,T2DM ,Single-nucleotide polymorphism ,Biology ,Biochemistry ,Polymorphism, Single Nucleotide ,White People ,Molecular Bases of Health & Disease ,03 medical and health sciences ,single nucleotide polymorphisms ,0302 clinical medicine ,Insulin resistance ,Asian People ,Risk Factors ,Internal medicine ,medicine ,Humans ,Genetic Predisposition to Disease ,030212 general & internal medicine ,Allele ,Risk factor ,Promoter Regions, Genetic ,Molecular Biology ,Alleles ,Genetic Association Studies ,Research Articles ,Diabetes & Metabolic Disorders ,Tumor Necrosis Factor-alpha ,Type 2 Diabetes Mellitus ,Cell Biology ,Odds ratio ,medicine.disease ,030104 developmental biology ,308G/A ,Diabetes Mellitus, Type 2 ,Meta-analysis ,TNF-α ,Mutation ,Software ,meta analysis - Abstract
Tumor necrosis factor-α (TNF-α) is involved in insulin resistance and has long been a candidate gene implicated in type 2 diabetes mellitus (T2DM), however the association between TNF-α polymorphisms -308G/A and -238G/A and T2DM remains controversial. The present study sought to verify associations between these polymorphisms and T2DM susceptibility using a meta-analysis approach. A total of 49 case–control studies were selected up to October 2018. Statistical analyses were performed by STATA 15.0 software. The odds ratios (ORs) and 95% confidence intervals were calculated to estimate associations. Meta-analyses revealed significant associations between TNF-α −308G/A and T2DM in the allele model (P=0.000); the dominant model (P=0.000); the recessive model (P=0.001); the overdominant model (P=0.008) and the codominant model (P=0.000). Subgroup analyses also showed associations in the allele model (P=0.006); the dominant model (P=0.004) and the overdominant model (P=0.005) in the Caucasian and in the allele model (P=0.007); the dominant model (P=0.014); the recessive model (P=0.000) and the codominant model (P=0.000) in the Asian. There were no associations between TNF-α −238G/A and T2DM in the overall and subgroup populations. Meta-regression, sensitivity analysis and publication bias analysis confirmed that results and data were statistically robust. Our meta-analysis suggests that TNF-α −308G/A is a risk factor for T2DM in Caucasian and Asian populations. It also indicates that TNF-α −238G/A may not be a risk factor for T2DM. More comprehensive studies will be required to confirm these associations.
- Published
- 2019
81. Identification of a pathogenic mutation in a Chinese pedigree with polycystic kidney disease
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Jie Wu, Rongwei Guan, Siqi Zhu, Guohua Ji, Xuelong Zhang, Wenjing Sun, Xianli Zhou, Jing Bai, Lidan Xu, Songbin Fu, Jiawei Sun, Xueyuan Jia, Hao Wang, Hui Su, Huanhuan Miao, Jingcui Yu, Kexian Dong, Han Wu, and Wei Ji
- Subjects
Adult ,0301 basic medicine ,Proband ,Cancer Research ,Candidate gene ,TRPP Cation Channels ,Genotype ,PKD1 ,duplication variant ,Biology ,urologic and male genital diseases ,Biochemistry ,PC1 ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Gene Duplication ,Exome Sequencing ,Gene duplication ,Genetics ,Polycystic kidney disease ,medicine ,Humans ,Missense mutation ,Genetic Predisposition to Disease ,Molecular Biology ,Genetic Association Studies ,Exome sequencing ,ADPKD ,Sanger sequencing ,Polycystic Kidney Diseases ,urogenital system ,pedigree ,Articles ,Middle Aged ,medicine.disease ,female genital diseases and pregnancy complications ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Mutation ,symbols ,Molecular Medicine ,Female - Abstract
Polycystic kidney disease (PKD) is a life‑threatening inherited disease with a morbidity of 1:500‑1,000 worldwide. Numerous progressively enlarging cysts are observed in the bilateral kidneys of patients with PKD, inducing structural damage and loss of kidney function. The present study analyzed one family with PKD. Whole exome sequencing of the proband was performed to detect the pathogenic gene present in the family. Candidate gene segments for lineal consanguinity in the family were amplified by nest polymerase chain reaction, followed by Sanger sequencing. One novel duplication variant (NM_001009944.2:c.9359dupA:p.Y3120_E3121delinsX) and one missense mutation (c.G9022A:p.V3008M) were detected in PKD1. Additionally, the pathogenic substitutions in PKD1 published from the dataset were analyzed. Following analysis and confirmation, the duplication variant NM_001009944.2:c.9359dupA:p.Y3120_E3121delinsX in PKD1, within the polycystin‑1, lipoxygenase, α‑toxin domain, was considered to be the pathogenic factor in the examined family with autosomal dominant PKD. Additionally, based on the analysis of 4,805 pathogenic substitutions in PKD1 within various regions, the presence of the missense mutation in the N‑terminal domain of polycystin‑1 may present high pathogenicity in ADPKD.
- Published
- 2019
82. The role of identity salience in creative thinking
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Ravi Mehta, Darren W. Dahl, and Lidan Xu
- Subjects
Salience (language) ,Creative thinking ,Psychology ,Social psychology - Published
- 2019
83. Associations between PTPN2 gene polymorphisms and psoriasis in Northeastern China
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Yuzhen Li, Yuandong Qiao, Jie Wu, Wenjing Sun, Chang Liu, Jingcui Yu, Jiawei Wu, Qiuyan Li, Xuelong Zhang, Lidan Xu, Songbin Fu, Qingbu Mei, and Xueyuan Jia
- Subjects
0301 basic medicine ,Adult ,Male ,China ,Adolescent ,Disease ,Protein tyrosine phosphatase ,Biology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Asian People ,Gene Frequency ,Psoriasis ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,Stat signaling ,Allele ,Child ,Gene ,Genetic Association Studies ,Aged ,Protein Tyrosine Phosphatase, Non-Receptor Type 2 ,Effector ,General Medicine ,Middle Aged ,medicine.disease ,030104 developmental biology ,030220 oncology & carcinogenesis ,Case-Control Studies ,Child, Preschool ,Immunology ,Etiology ,Female - Abstract
Psoriasis is a chronic immune-mediated disease with a complex etiology involving various genetic and immunological factors as well as environmental factors. Psoriasis is thought to be mediated by T-cells polarized to a Th17 fate. PTPN2 encodes the T-cell protein tyrosine phosphatase, which acts as a negative regulator of the JAK/STAT signaling pathways downstream of cytokines and plays a prominent role in T-cell activation, signaling and/or effector function. To evaluate the association between PTPN2 gene polymorphisms and psoriasis in the Northeastern Chinese population. A case-control study was conducted, and 398 patients with psoriasis and 397 healthy controls were genotyped for thirteen genetic polymorphisms in PTPN2. Allele analysis revealed that rs2847297, rs657555 and rs482160 polymorphisms were significantly associated with psoriasis (p = 0.0018, p = 0.0017 and p = 0.0086, respectively). Genotype analysis also revealed that these polymorphisms were significantly associated with psoriasis under different models (codominant, dominant and recessive models) (p 0.05). In this study, three haplotypes (H1, H7 and H11) were also found to be associated with psoriasis (p = 0.0015, p = 0.0094, and p = 0.0124, respectively). These results indicate that PTPN2 genetic polymorphisms are associated with psoriasis in the Northeastern Chinese population.
- Published
- 2018
84. Organotin(IV) complexes derived from Schiff base N’-[(1E)-(2-hydroxy-3-methoxyphenyl)methylidene]pyridine-3-carbohydrazone: Synthesis, in vitro cytotoxicities and DNA/BSA interaction
- Author
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Guoliang Chang, Jichun Cui, Yuanguang Yang, Lidan Xu, Min Hong, Dacheng Li, and Chen-Zhong Li
- Subjects
chemistry.chemical_classification ,Schiff base ,010405 organic chemistry ,Ligand ,Stereochemistry ,Organic Chemistry ,Intercalation (chemistry) ,Hydrazone ,Nuclear magnetic resonance spectroscopy ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Medicinal chemistry ,0104 chemical sciences ,Inorganic Chemistry ,chemistry.chemical_compound ,Trigonal bipyramidal molecular geometry ,chemistry ,Pyridine ,Materials Chemistry ,Chelation ,Physical and Theoretical Chemistry - Abstract
Five organotin(IV) hydrazone compounds have been synthesized from N’-[(1E)-(2-hydroxyl-3-methoxyphenyl)methylidene]pyridine-3-carbohydrazone and the corresponding organotin(IV). Solid state structures of five complexes were determined by elemental analysis, IR, NMR spectroscopy and for 1, 2, 3, 4 and 5 single crystal X-ray diffraction analysis. For compounds 4 and 5, structural analysis shows that each complex is a monomer with the tin atom five-coordinated in a distorted trigonal bipyramid chelating by the Schiff base ligand in an enolic tridentate fashion. Fascinatingly, in compounds 1, 2 and 3, structural analysis of them shows that each complex is a centrosymmetric trimers, in which the central Sn atom adopts six-coordinated geometry and the other Sn atom adopts five-coordinated geometry. In vitro cytotoxicities of five compounds on three human drug resistant tumor cells lines (A549, HeLa and MCF-7) were assessed by MTT assay. The interaction of the complexes with calf thymus DNA (CT-DNA) has been explored by absorption titration method, which revealed that complexes interact with CT-DNA through groove-binding and partial intercalation of the extended planar ligand with the DNA base stack. Furthermore, the protein fluorescence quenching studies reveal that there are strong binding interactions between compounds and bovine serum albumins (BSA). Studies reveal that di-n-butyltin(IV) complexes 2 and 4 with significant antiproliferative effects in the cells show stronger DNA/BSA interaction than the other complexes.
- Published
- 2016
85. In situ synthesized Au–Ag nanocages on graphene oxide nanosheets: a highly active and recyclable catalyst for the reduction of 4-nitrophenol
- Author
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Jifeng Liu, Fangli Wang, Shuling Xu, Min Hong, Haibo Li, Chen-Zhong Li, and Lidan Xu
- Subjects
Graphene ,Oxide ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Heterogeneous catalysis ,Borohydride ,01 natural sciences ,Catalysis ,0104 chemical sciences ,law.invention ,chemistry.chemical_compound ,Nanocages ,chemistry ,Chemical engineering ,law ,Materials Chemistry ,0210 nano-technology ,Hybrid material ,Graphene oxide paper - Abstract
Graphene oxide-supported hollow Au–Ag alloy nanocages (AuAgNC/GO) with tunable shell composition were in situ fabricated by employing a galvanic replacement reaction (GRR) between HAuCl4 and Ag nanoparticles loaded on graphene oxide nanosheets (AgNP/GO). AgNPs on the graphene oxide nanosheets were first synthesized as sacrificial templates by the microwave method. The as-synthesized hybrid materials were characterized by TEM, EDX, HAADF-STEM, XRD and XPS. On the well-known 4-nitrophenol (4-NP) reduction to 4-aminophenol (4-AP) in the presence of excess borohydride, AuAgNC/GO as a solid phase heterogeneous catalyst has been investigated, which exhibited a short induction time, high catalytic activity and good stability against agglomeration for the reduction.
- Published
- 2016
86. Associations between two single-nucleotide polymorphisms (rs1801278 and rs2943641) of insulin receptor substrate 1 gene and type 2 diabetes susceptibility: a meta-analysis
- Author
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Chuntao Wang, Guangfa Zhang, Yuandong Qiao, Lidan Xu, Xuelong Zhang, and Qiuyan Li
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Black People ,030209 endocrinology & metabolism ,Subgroup analysis ,Single-nucleotide polymorphism ,Type 2 diabetes ,Polymorphism, Single Nucleotide ,White People ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Asian People ,Polymorphism (computer science) ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Genetic Predisposition to Disease ,Genetic Association Studies ,Genetics ,business.industry ,medicine.disease ,Random effects model ,IRS1 ,030104 developmental biology ,Diabetes Mellitus, Type 2 ,Case-Control Studies ,Meta-analysis ,Insulin Receptor Substrate Proteins ,Female ,business ,Genome-Wide Association Study - Abstract
The objective of the study is to assess the association between rs1801278 and rs2943641 of insulin receptor substrate 1 gene (IRS1) and the susceptibility to type 2 diabetes. A literature search strategy was conducted to identify all references lists of relevant studies. The fixed or random effect model was used to calculate the pooled ORs on the basis of heterogeneity. Further analyses were performed to explore the sources of heterogeneity by sensitivity analysis, meta-regression analysis, and subgroup analysis. There was significant association between rs1801278 and type 2 diabetes risk in recessive model (AA vs. GA + GG, p = 0.043) and codominant model (AA vs. GG, p = 0.007). Subgroup analysis showed that the association between rs1801278 and type 2 diabetes risk was significant in dominant model (GA + AA vs. GG, p = 0.044), codominant model (GA vs. GG, p = 0.039), codominant model (AA vs. GG, p = 0.044), overdominant model (GG + AA vs. GA, p = 0.037) in Asian and codominant model (AA vs. GG, p = 0.039) in Caucasian of rs1801278. The association between rs2943641 and type 2 diabetes risk was significant in codominant model (CT vs. CC, p = 0.023) in Caucasian. This meta-analysis suggests that rs1801278 may play a role in type 2 diabetes risk, especially in Asian. It also indicates that rs2943641 may be associated with type 2 diabetes risk in Caucasian. Further larger studies should be performed to warrant confirmation.
- Published
- 2015
87. Additional file 1: Figure S1. of The distribution of three candidate cold-resistant SNPs in six minorities in North China
- Author
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Qiuyan Li, Kexian Dong, Lidan Xu, Xueyuan Jia, Wu, Jie, Wenjing Sun, Xuelong Zhang, and Songbin Fu
- Abstract
The location of populations. The six minority populations from Hezhen, Daur, Manchu, Korea, Mongolian and Ewenki are located in Heilongjiang Province (around 45°44’N126°39′E). CHB indicates Han Chinese in Beijing (around39°55’N116°27′E). CHS indicates Southern Han Chinese located in Sichuan province (around 30°22’N103°26′E). CDX indicates Chinese Dai in Xishuangbanna (around 22°3’N100°49′E). Greenlandic Inuit population live in area around 71°43’N42°24’W. Northeast Siberian population live in the northeast of Russia (around 78°50’N112°50′E). (DOCX 109 kb)
- Published
- 2018
- Full Text
- View/download PDF
88. Additional file 2: Table S1. of The distribution of three candidate cold-resistant SNPs in six minorities in North China
- Author
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Qiuyan Li, Kexian Dong, Lidan Xu, Xueyuan Jia, Wu, Jie, Wenjing Sun, Xuelong Zhang, and Songbin Fu
- Abstract
The frequencies of three polymorphisms in 11 populations Table S2 The P values for rs7115739 compared between six minorities in northern China and three populations in southern China. (DOCX 16Â kb)
- Published
- 2018
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89. Respite Service for the Elderly of the Belt and Road: A Zhejiang Story
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Lidan Xu, Wenjie Ge, Shiming Tang, and Changjuan Guo
- Subjects
Service (business) ,Nursing ,Respite care ,Business - Published
- 2018
90. Health Care for an Aging Society on the Belt and Road: A Zhejiang Story
- Author
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Changjuan Guo, Shiming Tang, Lidan Xu, and Wenjie Ge
- Subjects
Gerontology ,business.industry ,Health care ,Sociology ,Aging society ,business - Published
- 2018
91. The Study of Thermal, Mechanical and Shape Memory Properties of Chopped Carbon Fiber-Reinforced TPI Shape Memory Polymer Composites
- Author
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Jingbiao Liu, Jianming Guo, Lidan Xu, Zhenqing Wang, and Xiaoyu Sun
- Subjects
Materials science ,Polymers and Plastics ,Scanning electron microscope ,02 engineering and technology ,mechanical properties ,010402 general chemistry ,01 natural sciences ,Article ,lcsh:QD241-441 ,thermodynamics ,Differential scanning calorimetry ,lcsh:Organic chemistry ,polymer composite ,Fiber ,Composite material ,Tensile testing ,chemistry.chemical_classification ,shape memory properties ,General Chemistry ,Polymer ,Dynamic mechanical analysis ,Shape-memory alloy ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Shape-memory polymer ,chemistry ,0210 nano-technology - Abstract
Trans-l,4-polyisoprene (TPI) shape memory polymer composites with different chopped carbon fiber mass fractions were prepared to study the effects of different chopped carbon fiber mass fractions and temperatures on the TPI shape memory polymer composites in this paper. While guaranteeing the shape memory effect of TPI shape memory polymers, the carbon fiber fillers also significantly enhanced the mechanical properties of the polymers. The thermodynamic properties and shape memory properties of TPI shape memory polymers were studied by a differential scanning calorimeter (DSC) test, dynamic mechanical analysis (DMA) test, thermal conductivity test, static tensile test, mechanical cycle test, thermodynamic cycling test and shape memory test. Furthermore, the tensile fracture interface of TPI shape memory polymer composites was analyzed by scanning electron microscopy. The experimental results show that when the chopped carbon mass fraction fiber is 8%, TPI shape memory polymers have good shape memory properties and the best mechanical properties.
- Published
- 2017
- Full Text
- View/download PDF
92. Study of the effect of molecular structure and alkyl groups bound with tin(<scp>iv</scp>) on their cytotoxicity of organotin(<scp>iv</scp>) 2-phenyl-4-selenazole carboxylates
- Author
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Chen-Zhong Li, Min Hong, Yuanguang Yang, Lidan Xu, Dacheng Li, and Chuan Li
- Subjects
chemistry.chemical_classification ,chemistry ,Stereochemistry ,General Chemical Engineering ,Carboxylic acid ,Tetrahedral molecular geometry ,Molecule ,General Chemistry ,Nuclear magnetic resonance spectroscopy ,Crystal structure ,Cytotoxicity ,Alkyl ,In vitro - Abstract
Five organotin(IV) compounds Ph3SnL (1), (R2Sn)4O2L4 [R = n-Bu (2), n-Oct (3)], (R2Sn)4O2L2Cl2 [R = n-Bu (4), Me (5)], have been synthesized from the reactions of 2-phenyl-4-selenazole carboxylic acid (HL) with the corresponding organotin(IV) oxide or chlorides. These compounds have been characterized by elemental analysis, IR, 1H, 13C and 119Sn NMR spectroscopy, and single crystal X-ray diffraction analysis. Structural studies reveal that compound 1 exhibits a mononuclear four-coordinated tetrahedral geometry. Differently, the crystal structures of compounds 2–5 reveal the formation of the tetranuclear species containing a planar Sn4O4 core. All compounds were screened for their in vitro cytotoxic activities toward three cancer cell lines (Caco-2, A549, and HCT-116) and one normal rat hepatocyte cell line (BRL). The results indicate that both di-n-butyltin(IV) and triphenyltin(IV) derivatives not only show excellent cytotoxic activities on cisplatin-sensitive lung cancer cell line A549 and but also exhibit good cytotoxicity against cisplatin-insensitive colon cancer cell lines HCT-116 and Caco-2. Whereas, dimethyltin(IV) and di-n-octyltin(IV) complexes exhibit lower or no cytotoxic activity. The structure–activity relationship of the cytotoxicity of the title complexes has also been discussed.
- Published
- 2015
93. RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments
- Author
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Jie Wu, Rongwei Guan, Yan Jin, Lidan Xu, Yang Yu, Wenjing Sun, Jing Bai, Feng Chen, Zehua Bian, Songbin Fu, and Chao Quan
- Subjects
Ribosomal Proteins ,Cancer Research ,Paclitaxel ,Transcription, Genetic ,RNA Stability ,Cell ,Biology ,Biochemistry ,Transcription (biology) ,Cell Line, Tumor ,Reference genes ,Genetics ,medicine ,Humans ,RNA, Messenger ,Molecular Biology ,Gene ,Ovarian Neoplasms ,Oncogene ,Gene Expression Profiling ,Computational Biology ,Cell cycle ,Antineoplastic Agents, Phytogenic ,Molecular biology ,Gene expression profiling ,medicine.anatomical_structure ,Oncology ,Cancer cell ,Molecular Medicine ,Camptothecin ,Female - Abstract
Gene transcription analysis is important in cancer research, and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) has been demonstrated to be an effective method to evaluate gene transcription in cancer. RT‑qPCR requires an internal reference gene with a consistent level of mRNA transcription across various experimental conditions. However, it has been suggested that different treatments, including anticancer therapy, may influence the transcriptional stability of internal reference genes. Paclitaxel (PTX) and 10‑hydroxycamptothecin (HCPT) are widely used to treat various types of cancer, and a suitable internal reference gene is required in order to analyze the transcription profiles of the cells following treatment. In the current study, the transcriptional stability of 30 candidate reference genes was investigated in cancer cells following treatment with PTX and HCPT. The two ovarian cancer cell lines, UACC‑1598 and SKOV3, were treated with PTX and HCPT for 24 and 48 h, and the transcriptional levels of the candidate reference genes were subsequently evaluated by RT‑qPCR analysis. The transcriptional stability of the selected genes was then analyzed using qbase+ and NormFinder software. A total of 9 genes were demonstrated to exhibit high transcriptional stability and one of these genes, ribosomal protein L13a (RPL13A), was identified to exhibit high transcriptional stability in every group. The current study identified various reference genes suitable under different circumstances, while RPL13A was indicated to be the most suitable reference gene for analyzing the transcription profile of ovarian cancer cells following treatment with PTX and HCPT.
- Published
- 2014
94. Effect of a Roughness Element on the Hypersonic Boundary Layer Receptivity Due to Different Types of Free-Stream Disturbance with a Single Frequency
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Hongqing Lv, Mingfang Shi, Zhenqing Wang, and Lidan Xu
- Subjects
hypersonic boundary layer ,Hypersonic speed ,Fast Fourier transform ,Receptivity ,General Physics and Astronomy ,lcsh:Astrophysics ,02 engineering and technology ,Surface finish ,01 natural sciences ,Article ,FFT ,010305 fluids & plasmas ,0203 mechanical engineering ,lcsh:QB460-466 ,0103 physical sciences ,Physics::Chemical Physics ,lcsh:Science ,Physics ,020301 aerospace & aeronautics ,freestream disturbances ,Finite difference method ,Acoustic wave ,Mechanics ,lcsh:QC1-999 ,Boundary layer ,Amplitude ,lcsh:Q ,lcsh:Physics ,receptivity - Abstract
The hypersonic flow field around a blunt cone was simulated using a high-order finite difference method. Fast acoustic waves, slow acoustic waves, entropy waves, and vortical waves were introduced into the free-stream to determine the influence of a free-stream with disturbances on the hypersonic flow field and boundary layer. The effect of disturbance type on the evolution of perturbations in the hypersonic boundary layer was analyzed. Fast Fourier Transform was adopted to analyze the effect of the disturbance type on the evolution of different modes in the boundary layer. A roughness element was introduced into the flow field to reveal the impact of the roughness element on hypersonic boundary layer receptivity. The results showed that a free-stream with disturbances affected the hypersonic flow field and boundary layer, acoustic waves had the greatest influence. The impact of slow acoustic waves on the flow field was mainly concentrated in the region between the shock and the boundary layer, whereas the influence of fast acoustic waves was mainly concentrated in the boundary layer. Multi-mode perturbations formed in the boundary layer were caused by the free-stream with disturbances, wherein the fundamental mode was the dominant mode of the perturbations in the boundary layer caused by fast acoustic waves, entropy waves, and vortical waves. The dominant modes of the perturbations in the boundary layer caused by slow acoustic waves were both the fundamental mode and the second harmonic mode. The roughness element changed the propagation process of different modes of perturbations in the boundary layer. In the downstream region of the roughness element, perturbations in the boundary layer caused by the slow acoustic waves had the greatest influence. The second harmonic mode in the boundary layer was significantly suppressed, and the fundamental mode became the dominant mode. The effects of fast acoustic waves and entropy waves on the boundary layer receptivity were similar, except the amplitude of the perturbations in the boundary layer caused by the fast acoustic waves was larger.
- Published
- 2019
95. Effect of a New Grain Refiner (Al–Ti–Mg–Ce) on Hardness, Tensile, and Impact Properties of Al–7Si Alloy
- Author
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Mingfang Shi, Jitao Zhao, Lidan Xu, and Zhenqing Wang
- Subjects
lcsh:TN1-997 ,Materials science ,microstructure ,Alloy ,aluminum-silicon alloy ,02 engineering and technology ,engineering.material ,01 natural sciences ,Al–Ti–Mg–Ce ,0103 physical sciences ,Ultimate tensile strength ,General Materials Science ,lcsh:Mining engineering. Metallurgy ,Eutectic system ,Refining (metallurgy) ,010302 applied physics ,Impact toughness ,Metallurgy ,Metals and Alloys ,refine ,SHPB ,Strain rate ,021001 nanoscience & nanotechnology ,Microstructure ,mechanical property ,engineering ,0210 nano-technology ,Bar (unit) - Abstract
Through studying the effects of the Al&ndash, 5Ti-x(Mg&ndash, 30%Ce) refiner on the microstructure of the Al&ndash, 7Si alloy, we investigated the hardness, tensile, and impact properties of the Al&ndash, Si alloy before and after refinement. The refinement results show that Mg&ndash, 30%Ce can improve the refinement effect of Al&ndash, 5Ti on Al&ndash, 7Si, and also refine &alpha, Al and eutectic Si in Al&ndash, 7Si. When 2%Mg&ndash, 30%Ce is added, the refining effect of the aluminum&ndash, silicon alloy is most obvious. After refinement, the hardness and tensile properties of the Al&ndash, 7Si alloy improve. When 2%Mg&ndash, 30%Ce is added to the refiner, the tensile properties of the Al&ndash, Si alloy are the best. Dynamic mechanical tests were conducted on refined Al&ndash, 7Si alloy. The impact toughness of Al&ndash, 7Si&ndash, (Al&ndash, 5Ti-2(Mg&ndash, 30%Ce) improved and the impact toughness reached 34.91 J/cm2, which is 78.7% higher than that of Al&ndash, 5Ti) specimens. The separate Hopkinson pressure bar test results show that, under a high strain rate, the strain rate sensitivity of the prepared Al&ndash, Si alloy is significant. The specimens of Al&ndash, 30%Ce) demonstrated the best anti-impact performance at the same velocity.
- Published
- 2019
96. Combinatorial approach of in silico and in vitro evaluation of MLH1 variant associated with Lynch syndrome like metastatic colorectal cancer.
- Author
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Saleem, Komal, Zaib, Tahir, Wei Ji, Chunhui Zhang, Qian Qin, Yusi Wang, Lidan Xu, Hanfei Yu, Siqi Zhu, Kexian Dong, Shuhan Si, Xueyuan Jia, Jie Wu, Songbin Fu, and Wenjing Sun
- Subjects
HEREDITARY nonpolyposis colorectal cancer ,COLORECTAL cancer ,DNA mismatch repair ,METASTASIS ,GENETIC testing ,GENETIC counseling - Abstract
Colorectal cancer (CRC) is the third most developing cancer worldwide and Lynch syndrome (LS) accounts for 3-4% of CRC. Genetic alteration in any of DNA mismatch repair (MMR) gene is the major cause of LS that disrupt the normal upstream and downstream MMR events. Germline mutation of MLH1 in heterozygous state have an increased risk for CRC. Defective MMR pathway mostly results in microsatellite instability (MSI) that occurs in high percentage of CRC associated tumors. Here, we reported a patient with LS like metastatic CRC (mCRC) associated with other related cancers. Whole exome sequencing (WES) of the proband was performed to identify potential causative gene. Genetic screening validated by Sanger sequencing identified a heterozygous missense mutation in exon 12 of MLH1 (c.1151T>A, p.V384D). The clinical significance of identified variant was elucidated on the basis of clinicopathological data, computational predictions and various in vitro functional analysis. In silico predictions classified the variant to be deleterious and evolutionary conserved. In vitro functional studies revealed a significant decrease in protein expression because of stability defect leading to loss of MMR activity. Mutant residue found in MutL transducer domain of MLH1 that localized in the nucleus but translocation was not found to be significantly disturbed. In conclusion, our study give insight into reliability of combinatorial prediction approach of in silico and in vitro expression analysis. Hence, we highlighted the pathogenic correlation of MLH1 variant with LS associated CRC as well as help in earlier diagnosis and surveillance for improved management and genetic counselling. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
97. SNPs in folate pathway are associated with the risk of nonsyndromic cleft lip with or without cleft palate, a meta-analysis.
- Author
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Qiuyan Li, Lidan Xu, Xueyuan Jia, Komal Saleem, Tahir Zaib, Wenjing Sun, and Songbin Fu
- Subjects
- *
CLEFT lip , *CLEFT palate , *FOLIC acid , *FOLIC acid metabolism , *GENETIC models , *HUMAN abnormalities - Abstract
Background: Prenatal intake of folic acid is important for prevention of NSCL/P (nonsyndromic cleft lip with or without cleft palate). Associated genes in folate pathway are major enzymes of folic acid metabolism that is crucial for preventing birth defects. The present meta-analysis aims to investigate the association between four SNPs in folate pathway genes and the risk of NSCL/P. Methods: Comprehensive bioinformatics analysis was used to predict the functional pathogenicity of genetic variation. The PubMed, Embase database and Google Scholar were searched by two researchers. Stata 11.0 software was used to analyze the results. Subgroup analysis was carried out to assess the influence of genetic background. Sensitivity analysis, regression analysis and publication analysis were also conducted to enhance the strength of our results. Results: It is estimated that the probability of two missense mutation rs1801133 in MTHFR and rs1801394 in MTRR are more likely to be damaging by bioinformatics analysis. A significant association between rs1801133 and risk of NSCL/P in two genetic models: TT genotype vs CC genotype (OR = 1.333 95%CI = 1.062-1.674, P = 0.013), and recessive model (OR = 1.325 95%CI = 1.075-1.634, P = 0.008). A significant protective association between rs1801394 GG genotype and NSCL/P in Asian (GG vs AA, OR = 0.520 95%CI = 0.321-0.841, P = 0.008) was observed. Meta-regression, sensitivity analysis, and publication bias analysis confirmed that the results of the present study were statistically significant. Conclusions: The present study identified that rs1801133 in MTHFR is associated with the risk of NSCL/P, and rs1801394 Gg genotype in MTRR play a protective role in Asian. Further, larger studies should be performed to confirm these findings. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
98. The association of TNF-α-308G/A and -238G/A polymorphisms with type 2 diabetes mellitus: a meta-analysis.
- Author
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Xiaoliang Guo, Chenxi Li, Jiawei Wu, Qingbu Mei, Chang Liu, Wenjing Sun, Lidan Xu, and Songbin Fu
- Subjects
TUMOR necrosis factors ,META-analysis ,TYPE 2 diabetes - Abstract
Tumor necrosis factor-α (TNF-α) is involved in insulin resistance and has long been a candidate gene implicated in type 2 diabetes mellitus (T2DM), however the association between TNF-α polymorphisms -308G/A and -238G/A and T2DM remains controversial. The present study sought to verify associations between these polymorphisms and T2DM susceptibility using ameta-analysis approach. A total of 49 case-control studies were selected up to October 2018. Statistical analyses were performed by STATA 15.0 software. The odds ratios (ORs) and 95% confidence intervals were calculated to estimate associations. Meta-analyses revealed significant associations between TNF-α-308G/A and T2DM in the allele model (P=0.000); the dominant model (P=0.000); the recessive model (P=0.001); the overdominant model (P=0.008) and the codominant model (P=0.000). Subgroup analyses also showed associations in the allele model (P=0.006); the dominant model (P=0.004) and the overdominant model (P=0.005) in the Caucasian and in the allele model (P=0.007); the dominant model (P=0.014); the recessive model (P=0.000) and the codominant model (P=0.000) in the Asian. There were no associations between TNF-α-238G/A and T2DM in the overall and subgroup populations. Meta-regression, sensitivity analysis and publication bias analysis confirmed that results and data were statistically robust. Our meta-analysis suggests that TNF-α-308G/A is a risk factor for T2DM in Caucasian and Asian populations. It also indicates that TNF-α-238G/A may not be a risk factor for T2DM. More comprehensive studies will be required to confirm these associations. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
99. The polymorphisms of MSH6 gene are associated with AIDS progression in a northern Chinese population
- Author
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Ning He, Xueyuan Jia, Songbin Fu, Xuelong Zhang, Yuandong Qiao, Chunyan Zhao, Haiming Sun, Guangfa Zhang, Qiuyan Li, Lidan Xu, Chuntao Wang, and Jingcui Yu
- Subjects
0301 basic medicine ,Microbiology (medical) ,Adult ,CD4-Positive T-Lymphocytes ,Male ,Adolescent ,Gene Expression ,Single-nucleotide polymorphism ,Biology ,Microbiology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Gene Frequency ,Genotype ,Genetics ,Humans ,Genetic Predisposition to Disease ,Allele ,Homosexuality, Male ,Molecular Biology ,Allele frequency ,Ecology, Evolution, Behavior and Systematics ,Alleles ,Aged ,Acquired Immunodeficiency Syndrome ,Clinical Trials as Topic ,Haplotype ,Middle Aged ,CD4 Lymphocyte Count ,MSH6 ,DNA-Binding Proteins ,030104 developmental biology ,Infectious Diseases ,Haplotypes ,MSH2 ,030220 oncology & carcinogenesis ,Case-Control Studies ,Immunology ,Disease Progression ,HIV-1 ,DNA mismatch repair - Abstract
It has been reported that DNA repair genes play an important role in HIV-1 infection and AIDS progression. One DNA repair pathway, the mismatch repair (MMR) is associated with a wide variety of tumors. However, the role of single nucleotide polymorphisms (SNPs) in the MMR genes and their importance in HIV-1 infection and AIDS progression remain unclear. In the present study, 479 HIV-1-infected and 487 healthy individuals from northern China were genotyped for nine SNPs in the MSH2 gene (rs13019654, rs4608577, rs4952887, rs6726691, rs10191478, rs12999145, rs1981929, rs2042649, rs2303428) and five SNPs in the MSH6 gene (rs2348244, rs3136245, rs3136329, rs2072447, rs7562048). Our results showed that the rs7562048 G allele frequency was significantly higher in the cases with the CD4+ T-lymphocyte count 200 cells/μl (P = 0.001, OR = 1.811, 95% CI 1.255–2.614), which is in agreement with the result of the Bonferroni correction. The frequencies of the rs2348244 C allele and rs3136245 T allele were higher in the cases at clinical phase IV than those at clinical phase I + II + III (P = 0.026, OR = 1.591, 95% CI 1.056–2.398 and P = 0.019, OR = 1.749, 95% CI 1.096–2.791, respectively); however, this difference is not supported by the Bonferroni correction. There were no significant differences in the frequency of allele, genotype and haplotype of the 14 SNPs between HIV-1-infected individuals and healthy controls (P > 0.05). These results suggest that the rs7562048 is associated with the clinical features and that the MSH6 gene polymorphisms likely play an important role in the progression of AIDS in the northern Chinese population.
- Published
- 2016
100. A direct assay of carboxyl-containing small molecules by SALDI-MS on a AgNP/rGO-based nanoporous hybrid film
- Author
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Chen-Zhong Li, Lidan Xu, Min Hong, Huaisheng Wang, Haibo Li, Zhirong Geng, and Fangli Wang
- Subjects
Materials science ,Oxide ,Analytical chemistry ,02 engineering and technology ,010402 general chemistry ,Mass spectrometry ,01 natural sciences ,Biochemistry ,Silver nanoparticle ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,law ,Desorption ,Electrochemistry ,Environmental Chemistry ,Spectroscopy ,Nanocomposite ,Nanoporous ,Graphene ,021001 nanoscience & nanotechnology ,Small molecule ,0104 chemical sciences ,Chemical engineering ,chemistry ,0210 nano-technology - Abstract
Silver nanoparticles (AgNPs) and reduced graphene oxide (rGO) hybrid nanoporous structures fabricated by the layer-by-layer (LBL) electrostatic self-assembly have been applied as a simple platform for the rapid analysis of carboxyl-containing small molecules by surface-assisted laser desorption/ionization (D/I) mass spectrometry (SALDI-MS). By the simple one-step deposition of analytes onto the (AgNP/rGO)9 multilayer film, the MS measurements of various carboxyl-containing small molecules (including amino acids, fatty acids and organic dicarboxylic acids) can be done. In contrast to other energy transfer materials relative to AgNPs, the signal interferences of a Ag cluster (Agn(+) or Agn(-)) and a C cluster (Cn(+) or Cn(-)) have been effectively reduced or eliminated. The effects of various factors, such as the pore structure and composition of the substrates, on the efficiency of D/I have been investigated by comparing with the (AgNP)9 LBL nanoporous structure, (AgNP/rGO)9/(SiO2NP)6 LBL multilayer film and AgNP/prGO nanocomposites.
- Published
- 2016
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