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3,175 results on '"Lippard, Stephen J."'

55. Mobile zinc as a modulator of sensory perception

Author

Massachusetts Institute of Technology. Department of Chemistry, Goldberg, Jacob M, Lippard, Stephen J, Massachusetts Institute of Technology. Department of Chemistry, Goldberg, Jacob M, and Lippard, Stephen J

Abstract

Mobile zinc is an abundant transition metal ion in the central nervous system, with pools of divalent zinc accumulating in regions of the brain engaged in sensory perception and memory formation. Here, we present essential tools that we developed to interrogate the role(s) of mobile zinc in these processes. Most important are (a) fluorescent sensors that report the presence of mobile zinc and (b) fast, Zn-selective chelating agents for measuring zinc flux in animal tissue and live animals. The results of our studies, conducted in collaboration with neuroscientist experts, are presented for sensory organs involved in hearing, smell, vision, and learning and memory. A general principle emerging from these studies is that the function of mobile zinc in all cases appears to be downregulation of the amplitude of the response following overstimulation of the respective sensory organs. Possible consequences affecting human behavior are presented for future investigations in collaboration with interested behavioral scientists.

Published
2023

68. Identification and Rescue of α-Synuclein Toxicity in Parkinson Patient-Derived Neurons

Author

Chung, Chee Yeun, Khurana, Vikram, Auluck, Pavan K., Tardiff, Daniel F., Mazzulli, Joseph R., Söldner, Frank, Baru, Valeriya, Lou, Yali, Freyzon, Yelena, Cho, Sukhee, Mungenast, Alison E., Muffat, Julien, Mitalipova, Maisam, Pluth, Michael D., Jui, Nathan T., Schüle, Birgitt, Lippard, Stephen J., Tsai, Li-Huei, Krainc, Dimitri, Buchwald, Stephen L., Jaenisch, Rudolf, and Lindquist, Susan

Published
2013
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76. Sensing intracellular calcium ions using a manganese-based MRI contrast agent

Author

Barandov, Ali, Bartelle, Benjamin B., Williamson, Catherine G., Loucks, Emily S., Lippard, Stephen J., Jasanoff, Alan, Barandov, Ali, Bartelle, Benjamin B., Williamson, Catherine G., Loucks, Emily S., Lippard, Stephen J., and Jasanoff, Alan

Abstract

© 2019, The Author(s). Calcium ions are essential to signal transduction in virtually all cells, where they coordinate processes ranging from embryogenesis to neural function. Although optical probes for intracellular calcium imaging have been available for decades, the development of probes for noninvasive detection of intracellular calcium signaling in deep tissue and intact organisms remains a challenge. To address this problem, we synthesized a manganese-based paramagnetic contrast agent, ManICS1-AM, designed to permeate cells, undergo esterase cleavage, and allow intracellular calcium levels to be monitored by magnetic resonance imaging (MRI). Cells loaded with ManICS1-AM show changes in MRI contrast when stimulated with pharmacological agents or optogenetic tools; responses directly parallel the signals obtained using fluorescent calcium indicators. Introduction of ManICS1-AM into rodent brains furthermore permits MRI-based measurement of neural activation in optically inaccessible brain regions. These results thus validate ManICS1-AM as a calcium sensor compatible with the extensive penetration depth and field of view afforded by MRI.

Published
2022

100. Mobile zinc as a modulator of sensory perception.

Author

Goldberg, Jacob M. and Lippard, Stephen J.

Subjects
*SENSORY perception, *SENSE organs, *ZINC, *TRANSITION metal ions, *CHELATING agents
Abstract

Mobile zinc is an abundant transition metal ion in the central nervous system, with pools of divalent zinc accumulating in regions of the brain engaged in sensory perception and memory formation. Here, we present essential tools that we developed to interrogate the role(s) of mobile zinc in these processes. Most important are (a) fluorescent sensors that report the presence of mobile zinc and (b) fast, Zn‐selective chelating agents for measuring zinc flux in animal tissue and live animals. The results of our studies, conducted in collaboration with neuroscientist experts, are presented for sensory organs involved in hearing, smell, vision, and learning and memory. A general principle emerging from these studies is that the function of mobile zinc in all cases appears to be downregulation of the amplitude of the response following overstimulation of the respective sensory organs. Possible consequences affecting human behavior are presented for future investigations in collaboration with interested behavioral scientists. [ABSTRACT FROM AUTHOR]

Published
2023
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