51. Study of the Method for the Determination of Bisphenol A in Vegetable Oil by High Performance Liquid Chromatography
- Author
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LIU Yong-ying, JIANG Wen-jia, HU Rong, and WU Xian-shao
- Subjects
bisphenol a ,vegetable oil ,high performance liquid chromatogrphy ,content determination ,method validation ,Food processing and manufacture ,TP368-456 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Through the research on the determination of bisphenol A in vegetable oil by high-performance liquid chromatography, this article established the method for determining bisphenol A in vegetable oil. Bisphenol A was extracted with acetonitrile.The extract was purified and eluted by the amino solid-phase extraction column and then concentrated and reconstituted. After filtration, the extract was separated by high-performance liquid chromatography, detected by the fluorescence detector, and quantified by the standard external method. The methodological performance analysis showed that the linear range was 0.003 333~0.666 7 μg/mL, the correlation coefficient (r) of the standard curve is greater than 0.999, the detection limit of the method is 0.007 mg/kg, see the quantification limit was 0.013 mg/kg, the recoveries ranged from 90.8% to 103.2%, and the coefficient of variation of seven replicate assays was 2.8%. It was verified that the method had good linearity, high sensitivity, good repeatability, accurate and reliable results, and could meet the requirements of GB/T 27417—2017, providing a technical basis for the scientific evaluation of the content of bisphenol A in vegetable oil.
- Published
- 2022
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