Search

In Citizen Science, members of the general public collaborate with scientists to generate and use data relating to the natural world. For the many fields of marine research, this is a particularly powerful approach which should not be overlooked. The sheer scale of coastal and ocean environments mean that it would take several lifetimes for scientists to study them alone. By collaborating with citizens, a much greater number of people can be mobilized to gather a wealth of data and develop new scientific knowledge and understanding. The variety of data types which are amenable to Citizen Science, as outlined in the position paper, are great, meaning that there could be a project to suit everyone. Citizen Science can also enable participants to improve their Ocean Literacy, gain new skills and experiences, and can also empower them to participate in the process of delivering future marine policy. Now, more than ever, marine science research is needed to understand the impacts of a world undergoing change. The rise of Marine Citizen Science to help address this need is therefore timely. This paper highlights opportunities, challenges and best practice in Marine Citizen Science, and sets out a list of high-level strategic recommendations for the future development of Marine Citizen Science in Europe. It presents examples of existing Marine Citizen Science initiatives in Europe to illustrate good practice. Common concerns such as data quality and maintaining engagement are discussed, as are future opportunities such as increased use of technology and potential role of Marine Citizen Science in informing marine policy and conservation. The paper closes with a list of high-level strategic recommendations for the future development of Marine Citizen Science in Europe.

The Ross Sea is one of the most productive areas in the Southern Ocean, and is characterized by a pronounced export of particulate organic carbon to the mesopelagic (up to 50% of surface primary production). This organic carbon downward flux is deeply regulated by its interactions with prokaryotic communities within the entire water column. During austral summer 2017, we performed four microcosm experiments by providing freshly produced algal detritus to free-living prokaryotic communities (

In the Mediterranean, Cystoseira sensu lato (s.l.) (Phaeophyceae) forests have sharply declined and restoration measures are needed to compensate for the loss. Assisted regeneration through the outplanting of seedlings grown ex-situ has proven to be a sustainable option. Optimizing mesocosm culture can maximize survival of the most critical embryonic stages and reduce long-term maintenance costs. Host-microbiome interactions could also play a crucial role in seedling development and welfare. In this context, we aimed to advance a cultivation protocol that stimulates the growth and fitness of Ericaria amentacea (Phaeophyceae) seedlings and identify the associated microbial biofilm communities. Seedlings were cultured in 6 treatments [i.e., filtered seawater (SW, C, Control), von Stoch-enriched SW (VS), VS + algal extract (VSA), algal extract-enriched SW: A1< A2< A3]. After the field, A2 seedlings had the highest cover (1372 ± 53.66 mm2), which was 1.8 and 1.9 times greater than in VS and VSA, respectively. The addition of the algal extract and nutrients significantly affected the structure and composition of the microbial community that shifted over time in each culture medium. We identified a treatment-specific microbial fingerprint. After the mesocosm phase, A2 was characterized by 4 unique taxa: Postechiella, Winogradskyella, Roseovarius and Arenibacter (Bacteria). Given the success of A2 seedlings, we propose the probiotic consortia candidates characterized by the unique treatment-taxa in conjunction with the shared taxon Psychroserpens (Bacteria, present in A1, A2, VSA, VS) and the reminder community. Within the holobiont concept, the effect of algal extract or nutrients on the algae and/or biofilm could have important consequences for tuning the overall interaction networks. Our study has shown that macroalgal restoration could benefit from both the use of commercial algal extract and tailored nutrient enrichment in ex-situ cultures and the identification of probiotic consortia candidates that promote seedling growth.

Chesapeake Bay (CB) and the northern Adriatic Sea (NAS) exhibit contrasting patterns in trophic status and thereby offer contrasting case studies in microbial ecology. In the surface mixed layer in both systems, the abundance and productivity of cyanobacteria and of heterotrophic bacteria exhibit summer maxima and winter minima. Available evidence implicates temperature as a major constraint during winter and spring, and supply of nutrients and/or dissolved organic carbon during summer. Bacterioplankton density is an order of magnitude higher in CB than in the NAS, while productivity varies over similar ranges in both systems, suggesting higher bacterioplankton turnover in the NAS. Where data exist, phages seem to play an important role in constraining cyanobacterial and heterotrophic bacterioplankton abundance. The ratio of viral‐like particles to bacteria was higher in the NAS, however, which may explain the higher turnover rate of heterotrophic bacteria. In the NAS, bacterial abundance has been in decline since the early 2000s, associated with declining nutrient loading, while changes in CB have not been documented. Projected climate‐driven changes in riverine nutrient loading and increases in the duration of the growing season are likely to increase contrasts observed in the microbial ecology of the two systems.

Particulate organic matter (POM) export represents the underlying principle of the biological carbon pump, driving the carbon flux from the sunlit to the dark ocean. The efficiency of this process is tightly linked to the prokaryotic community, as >70% of POM respiration is carried out by particle-associated prokaryotes. In the Ross Sea, one of the most productive areas of the Southern Ocean, up to 50% of the surface primary production is exported to the mesopelagic ocean as POM. Recent evidence suggests that a significant fraction of the POM in this area is composed of intact phytoplankton cells. During austral summer 2017, we set up bottle enrichment experiments in which we amended free-living surface and deep prokaryotic communities with organic matter pools generated from native microplankton, mimicking the particle export that may derive from mild (1 μg of Chlorophyll a L–1) and intense (10 μg of Chlorophyll a L–1) phytoplankton bloom. Over a course of 4 days, we followed free-living and particle-attached prokaryotes’ abundance, the degradation rates of polysaccharides, proteins and lipids, heterotrophic production as well as inorganic carbon utilization and prokaryotic community structure dynamics. Our results showed that several rare or undetected taxa in the initial community became dominant during the time course of the incubations and that different phytodetritus-derived organic matter sources induced specific changes in microbial communities, selecting for peculiar degradation and utilization processes spectra. Moreover, the features of the supplied detritus (in terms of microplankton taxa composition) determined different colonization dynamics and organic matter processing modes. Our study provides insights into the mechanisms underlying the prokaryotic utilization of phytodetritus, a significant pool of organic matter in the dark ocean.

Cells and tissues in our body are continuously subjected to mechanical stress. Mechanical stimuli, such as tensile and contractile forces, and shear stress, elicit cellular responses, including gene and protein alterations that determine key behaviors, including proliferation, differentiation, migration, and adhesion. Several tools and techniques have been developed to study these mechanobiological phenomena, including micro-electro-mechanical systems (MEMS). MEMS provide a platform for nano-to-microscale mechanical stimulation of biological samples and quantitative analysis of their biomechanical responses. However, current devices are limited in their capability to perform single cell micromechanical stimulations as well as correlating their structural phenotype by imaging techniques simultaneously. In this study, a biocompatible and optically transparent MEMS for single cell mechanobiological studies is reported. A silicon nitride microfabricated device is designed to perform uniaxial tensile deformation of single cells and tissue. Optical transparency and open architecture of the device allows coupling of the MEMS to structural and biophysical assays, including optical microscopy techniques and atomic force microscopy (AFM). We demonstrate the design, fabrication, testing, biocompatibility and multimodal imaging with optical and AFM techniques, providing a proof-of-concept for a multimodal MEMS. The integrated multimodal system would allow simultaneous controlled mechanical stimulation of single cells and correlate cellular response.