51. Solid lipid nanoparticles of anticancer drug andrographolide: formulation, in vitro and in vivo studies
- Author
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Sayeed Ahmad, Rabea Parveen, Mohammed Samim, Farhan Jalees Ahmad, and Zeenat Iqbal
- Subjects
Andrographolide ,Chemistry, Pharmaceutical ,Cmax ,Pharmaceutical Science ,Biological Availability ,Antineoplastic Agents ,Pharmacology ,chemistry.chemical_compound ,Mice ,Pharmacokinetics ,Suspensions ,In vivo ,Cell Line, Tumor ,Drug Discovery ,Solid lipid nanoparticle ,Animals ,Humans ,Particle Size ,Mice, Inbred BALB C ,Chromatography ,Organic Chemistry ,Area under the curve ,Water ,Lipids ,Bioavailability ,body regions ,chemistry ,Solubility ,Area Under Curve ,Lipophilicity ,MCF-7 Cells ,Solvents ,Nanoparticles ,Female ,Diterpenes - Abstract
Diterpenoidal anti-cancer drug andrographolide (AD) was encapsulated into solid lipid nanoparticle (SLN) because of poor aqueous solubility and high lipophilicity. AD-SLNs were prepared by solvent injection method and characterized for droplet size, surface morphology, zeta potential, etc. In vitro drug release was carried out by dialysis-membrane method. A pharmacokinetic study was performed by UPLC/Q-TOF-MS method to determine the maximum plasma concentration (Cmax), area under the curve (AUC), etc. There was an improvement in Cmax and AUC of AD-SLNs when compared with AD, thereby enhancing the bioavailability of AD. The tmax was increased than that of AD suspension, indicating the sustained release pattern of AD-SLNs. The antitumor activity was carried out on Balb/c mice showing better results with AD-SLNs as compared to AD. Thus, the AD-loaded SLNs would be useful for delivering poorly water-soluble AD with enhanced bioavailability and improved antitumor activity.
- Published
- 2013