51. Able to talk again? - A screen of gun1 suppressors to identify factors in chloroplast retrograde signaling
- Author
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Veciana, Nil and Monte, Elena
- Subjects
food and beverages - Abstract
Resumen del póster presentado al Congreso 'At the Forefront of Plant Research', celebrado en Barcelona (España) del 6 al 8 de mayo de 2019., Plant development is influenced by multiple internal and external factors. Among these, light is of utmost importance due to its function as source of energy and also as an information signal. After the first light exposure, seedlings undergo deetiolation, whereby development switches from skotomorphogenic to photomorphogenic, characterized by the opening and expansion of the cotyledons and chloroplast development to support autotrophic growth. This process is accompanied by a massive reprogramming of the transcriptome. However, an excess of light can cause chloroplast damage, triggering a retrograde signal (RS) to the nucleus that suppresses photomorphogenesis by inhibiting the switch in gene expression that takes place during deetiolation. In this process, the chloroplast-localized protein GENOMES UNCOUPLED1 (GUN1) is a key factor mediating the RS, as gun1 mutants do not show the suppression of photomorphogenesis after chloroplast damage. However, how GUN1 mediates the RS transmitted to the nucleus remains unclear. We are performing a gain-of-function approach using the FOX (Full-length cDNA Over-eXpressing) gene hunting system (a library of about 10.000 independent cDNAs expressed under the 35S promoter) to identify suppressors of the gun1 mutant phenotype. These suppressors will potentially correspond to factors downstream of GUN1 in the RS pathway that are able to restore the communication between the chloroplast and the nucleus in the gun1 mutant.
- Published
- 2019