51. Fast and sensitive detection of viable Escherichia coli O157:H7 using a microwell-confined and propidium monoazide-assisted digital CRISPR microfluidic platform.
- Author
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Yin, Weihong, Hu, Kai, Yu, Bingwen, Zhang, Tao, Mei, Haohua, Zhang, Bowen, Zou, Zheyu, Xia, Liping, Gui, Yehong, Yin, Juxing, Jin, Wei, and Mu, Ying
- Subjects
FOOD poisoning ,PROPIDIUM monoazide ,FOOD testing ,FOOD safety ,FOOD pathogens - Abstract
Escherichia coli O157:H7 is a major foodborne pathogen that poses a significant threat to food safety and human health. Rapid and sensitive detection of viable Escherichia coli O157:H7 can effectively prevent food poisoning. Here, we developed a microwell-confined and propidium monoazide-assisted digital CRISPR microfluidic platform for rapid and sensitive detection of viable Escherichia coli O157:H7 in food samples. The reaction time is significantly reduced by minimizing the microwell volume, yielding qualitative results in 5 min and absolute quantitative results in 15 min. With the assistance of propidium monoazide, this platform can eliminate the interference from 99% of dead Escherichia coli O157:H7. The direct lysis method obviates the need for a complex nucleic acid extraction process, offering a limit of detection of 3.6 × 10
1 CFU mL−1 within 30 min. Our results demonstrated that the platform provides a powerful tool for rapid detection of Escherichia coli O157:H7 and provides reliable guidance for food safety testing. [ABSTRACT FROM AUTHOR]- Published
- 2024
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