51. Production of tetra-methylpyrazine using engineered Corynebacterium glutamicum
- Author
-
Eng, Thomas, Sasaki, Yusuke, Herbert, Robin A, Lau, Andrew, Trinh, Jessica, Chen, Yan, Mirsiaghi, Mona, Petzold, Christopher J, and Mukhopadhyay, Aindrila
- Subjects
Biological Sciences ,Industrial Biotechnology ,Corynebacterium glutamicum ,Alkaloids ,Terpene ,2 ,3 ,5 ,6-Tetra-methylpyrazine ,Isopentenol ,Bioreactor ,Biochemistry and cell biology ,Medical biochemistry and metabolomics - Abstract
Corynebacterium glutamicum ATCC 13032 is an established and industrially-relevant microbial host that has been utilized for the expression of many desirable bioproducts. Tetra-methylpyrazine (TMP) is a naturally occurring alkylpyrazine with broad applications spanning fragrances to resins. We identified an engineered strain of C. glutamicum which produces 5 g/L TMP and separately, a strain which can co-produce both TMP and the biofuel compound isopentenol. Ionic liquids also stimulate TMP production in engineered strains. Using a fed batch-mode feeding strategy, ionic liquid stimulated strains produced 2.2 g/L of tetra-methylpyrazine. We show that feedback from a specific heterologous gene pathway on host physiology leads to acetoin accumulation and the production of TMP.
- Published
- 2020