51. Non contiguous-finished genome sequence and description of Bacillus jeddahensis sp. nov
- Author
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Bittar, Fadi, Bibi, Fehmida, Ramasamy, Dhamodharan, Lagier, Jean-Christophe, Azhar, Esam I., Jiman-Fatani, Asif A., Al-Ghamdi, Ahmed K., Nguyen, Ti Thien, Yasir, Muhammad, Fournier, Pierre-Edouard, Raoult, Didier, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), King Fahd Medical Research Center, Department of Medical Microbiology and Parasitology, King Abdulaziz University-Faculty of Medicine, Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), and Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Genome ,Culturomics ,Human feces ,Bacillus jeddahensis ,Taxono-genomics ,Extended Genome Report ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,ComputingMilieux_MISCELLANEOUS - Abstract
Strain JCET was isolated from the fecal sample of a 24-year-old obese man living in Jeddah, Saudi Arabia. It is an aerobic, Gram-positive, rod-shaped bacterium. This strain exhibits a 16S rRNA nucleotide sequence similarity of 97.5 % with Bacillus niacini, the phylogenetically closest species with standing nomenclature. Moreover, the strain JCET presents many phenotypic differences, when it is compared to other Bacillus species, and shows a low MALDI-TOF Mass Spectrometry score that does not allow any identification. Thus, it is likely that this strain represents a new species. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,762,944 bp long genome (1 chromosome but no plasmid) contains 4,654 protein-coding and 98 RNAs genes, including 92 tRNA genes. The strain JCET differs from most of the other closely Bacillus species by more than 1 % in G + C content. In addition, digital DNA-DNA hybridization values for the genome of the strain JCET against the closest Bacillus genomes range between 19.5 to 28.1, that confirming again its new species status. On the basis of these polyphasic data made of phenotypic and genomic analyses, we propose the creation of Bacillus jeddahensis sp. nov. that contains the strain JCET. Electronic supplementary material The online version of this article (doi:10.1186/s40793-015-0024-y) contains supplementary material, which is available to authorized users.
- Published
- 2015