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376 results on '"Pan, Hung-Chuan"'

67. The signals of FGFs on the neurogenesis of embryonic stem cells

Author

Lin Shinn-Zong, Lee Kun-Hsiung, Pan Hung-Chuan, Chiu Ing-Ming, Shen Shih-Cheng, Liu Chin-San, Chen Ching-Wen, and Su Hong-Lin

Subjects
Medicine
Abstract

Abstract Background Neural induction is a complex process and the detailed mechanism of FGF-induced neurogenesis remains unclear. Methods By using a serum-free neural induction method, we showed that FGF1 dose-dependently promoted the induction of Sox1/N-cadherin/nestin triple positive cells, which represent primitive neuroblasts, from mouse embryonic stem (ES) cells. Results We demonstrated that FGF1, FGF2, and FGF4, but not FGF8b, enhanced this neurogenesis. Especially, FGF-enhanced neurogenesis is not mediated through the rescue of the apoptosis or the enhancement of the proliferation of Sox1+ cells. We further indicated that the inactivation of c-Jun N-terminal kinase-1 (JNK-1) and extracellular signal-related kinase-2 (ERK-2), but not p38 mitogen-activated protein kinase (MAPK), inhibited the neural formation through the inhibition of ES differentiation, but not through the formation of endomesodermal cells. Conclusions These lines of evidence delineated the roles of FGF downstream signals in the early neural differentiation of ES cells.

Published
2010
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68. Escalated regeneration in sciatic nerve crush injury by the combined therapy of human amniotic fluid mesenchymal stem cells and fermented soybean extracts, Natto

Author

Pan Hung-Chuan, Yang Dar-Yu, Ho Shu-Peng, Sheu Meei-Ling, Chen Chung-Jung, Hwang Shiaw-Min, Chang Ming-Hong, and Cheng Fu-Chou

Subjects
Medicine
Abstract

Abstract Attenuation of inflammatory cell deposits and associated cytokines prevented the apoptosis of transplanted stem cells in a sciatic nerve crush injury model. Suppression of inflammatory cytokines by fermented soybean extracts (Natto) was also beneficial to nerve regeneration. In this study, the effect of Natto on transplanted human amniotic fluid mesenchymal stem cells (AFS) was evaluated. Peripheral nerve injury was induced in SD rats by crushing a sciatic nerve using a vessel clamp. Animals were categorized into four groups: Group I: no treatment; Group II: fed with Natto (16 mg/day for 7 consecutive days); Group III: AFS embedded in fibrin glue; Group IV: Combination of group II and III therapy. Transplanted AFS and Schwann cell apoptosis, inflammatory cell deposits and associated cytokines, motor function, and nerve regeneration were evaluated 7 or 28 days after injury. The deterioration of neurological function was attenuated by AFS, Natto, or the combined therapy. The combined therapy caused the most significantly beneficial effects. Administration of Natto suppressed the inflammatory responses and correlated with decreased AFS and Schwann cell apoptosis. The decreased AFS apoptosis was in line with neurological improvement such as expression of early regeneration marker of neurofilament and late markers of S-100 and decreased vacuole formation. Administration of either AFS, or Natto, or combined therapy augmented the nerve regeneration. In conclusion, administration of Natto may rescue the AFS and Schwann cells from apoptosis by suppressing the macrophage deposits, associated inflammatory cytokines, and fibrin deposits.

Published
2009
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71. Effect of refinement of skull surface measurement on Gamma knife radiosurgery

Author

Pan, Hung-Chuan, Chen, Yen-Ju, Shen, Chiung-Chi, Chen, Clayton Chi-Chang, Chen, Chung-Jung, and Sheehan, Jason M.D.

Subjects
Craniometry -- Usage, Craniometry -- Comparative analysis, Magnetic resonance imaging -- Usage, Magnetic resonance imaging -- Health aspects, Magnetic resonance imaging -- Comparative analysis, Radiosurgery -- Methods, Radiosurgery -- Health aspects, Radiosurgery -- Research, Health
Abstract

Objective: The calculation of radiation dose and estimation of a skull collision in Gamma Knife radiosurgery (GKRS) are based on measurement of the surface of skull by a skull scaling instrument. This study was conducted to evaluate the measurement discrepancy between the Gamma Knife (GK) skull scaling instrument and actual surface as determined by MRI. Methods: 168 consecutive GKRS patients were included. Discrepancies between those values computed using the skull scaling instrument and the actual MRI were expressed as the difference between the two divided by that measured by MRI. Results: The dosage discrepancy over the deep, middle, and top targets was 3.15 A [+ or -] 0.31%, 3.51 A [+ or -] 0.37%, and 4.49 A [+ or -] 0.41%, respectively (p Keywords: Gamma knife radiosurgery | Skull volume | Collision | Radiation dose, Introduction Gamma Knife has become an important tool in neurosurgery; it is commonly employed in the treatment of patient's with brain tumors, arteriovenous malformations, or functional disorders such as trigeminal [...]

Published
2009

74. Melatonin set out to ER stress signaling thwarts epithelial mesenchymal transition and peritoneal dissemination via calpain-mediated C/EBPβ and NFκ B cleavage

Author

Wu, Sheng-Mao, primary, Lin, Wan-Yu, additional, Shen, Chin-Chang, additional, Pan, Hung-Chuan, additional, Keh-Bin, Wang, additional, Chen, Yi-Ching, additional, Jan, Yee-Jee, additional, Lai, De-Wei, additional, Tang, Shu-Ching, additional, Tien, Hsing-Ru, additional, Chiu, Chien-Shan, additional, Tsai, Tsung-Chih, additional, Lai, Yi-Liang, additional, and Sheu, Meei-Ling, additional

Published
2015
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80. Outcome of hearing preservation related to tumor morphologic analysis in acoustic neuromas treated by gamma knife radiosurgery.

Author

Szu-Yen Pan, Shih-An Liu, Ming-Hsi Sun, Hsi-Kai Tsou, Shinh-Dung Lee, Yen-Ju Chen, Sheehan, Jason, Meei-Ling Sheu, Hung-Chuan Pan, Pan, Szu-Yen, Liu, Shih-An, Sun, Ming-Hsi, Tsou, Hsi-Kai, Lee, Shinh-Dung, Chen, Yen-Ju, Sheu, Meei-Ling, and Pan, Hung-Chuan

Subjects
ACOUSTIC neuroma, ACOUSTIC tumors, RADIOSURGERY, RADIOTHERAPY, NEUROSURGERY, HEARING, THERAPEUTICS, LONGITUDINAL method, TREATMENT effectiveness
Abstract

Background: Gamma Knife radiosurgery (GKRS) is an important part of the neurosurgical armamentarium in the treatment of acoustic neuromas. However, the treatment outcome related to the morphology of the tumor has not been rigorously studied. In this cohort, we evaluated the morphological features of the tumor in the tumor response and neurological outcomes after GKRS.Material and Methods: From July 2003 to December 2008, there were 93 cases of acoustic neuromas treated upfront with GKRS with 64 cases with serviceable hearing and 29 cases without serviceable hearing to fulfill the margin dose of 12Gy with at least follow up 5 years.Results: The duration of symptom before GKRS in serviceable /no serviceable hearing was 7.9 ± 1.2 and 15.3 ± 3.1 months (p < 0.001) and associated no-hearing symptom was 70% and 35%, respectively (p < 0.001). There was 81.2% of hearing preservation after GKRS in serviceable hearing group including 27 cases of pear type (84%), 14 of linear type (70%), and 9 cases of sphere type (90%) (p < 0.01); however, there was no case of hearing improvement in the no-serviceable hearing group (0 of 29). There were 85% of patients with decreased tinnitus in serviceable hearing groups as compared to 61.5% of patients in no serviceable hearing group (p < 0.05). In multivariate analysis, the tumor morphology was highly correlated to hearing preservation rate (p < 0.01).Conclusion: In the limited case of this cohort, we found that the tumor morphology and timing of treatment was highly correlated to the rate of hearing preservation. The sphere type of tumor morphology was associated with the best chance of hearing preservation. [ABSTRACT FROM AUTHOR]

Published
2017
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88. Exome Sequencing Identifies GNB4 Mutations as a Cause of Dominant Intermediate Charcot-Marie-Tooth Disease

Author

Soong, Bing-Wen, primary, Huang, Yen-Hua, additional, Tsai, Pei-Chien, additional, Huang, Chien-Chang, additional, Pan, Hung-Chuan, additional, Lu, Yi-Chun, additional, Chien, Hsin-Ju, additional, Liu, Tze-Tze, additional, Chang, Ming-Hong, additional, Lin, Kon-Ping, additional, Tu, Pang-Hsien, additional, Kao, Lung-Sen, additional, and Lee, Yi-Chung, additional

Published
2013
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89. Melatonin set out to ER stress signaling thwarts epithelial mesenchymal transition and peritoneal dissemination via calpain-mediated C/ EBP β and NF κB cleavage.

Author

Wu, Sheng‐Mao, Lin, Wan‐Yu, Shen, Chin‐Chang, Pan, Hung‐Chuan, Keh‐Bin, Wang, Chen, Yi‐Ching, Jan, Yee‐Jee, Lai, De‐Wei, Tang, Shu‐Ching, Tien, Hsing‐Ru, Chiu, Chien‐Shan, Tsai, Tsung‐Chih, Lai, Yi‐Liang, and Sheu, Meei‐Ling

Subjects
MELATONIN, ENDOPLASMIC reticulum, EPITHELIAL cells, MESENCHYMAL stem cells, CALPAIN, NF-kappa B
Abstract

Peritoneal dissemination of tumor has high mortality and is associated with the loss of epithelial features, acquisition of motile mesenchymal morphology characteristics, and invasive properties by tumor cells. Melatonin is an endogenously produced molecule in all plant species that is known to exert antitumor activity, but to date, its underlying mechanisms and antiperitoneal metastasis efficacy is not well defined. This study determined the antiperitoneal dissemination potential of melatonin in vivo and assessed its association with the inhibition of epithelial-to-mesenchymal transition ( EMT) signaling mechanism by endoplasmic reticulum ( ER) stress, which may be a major molecular mechanism of melatonin against cancer. The results demonstrate that melatonin inhibited peritoneal metastasis in vivo and activated ER stress in Cignal ERSE Reporter Assay, organelle structure in transmission electron microscopy images, calpain activity, and protein biomarkers like p-elf2 α. Moreover, the overexpression of transcription factor C/ EBP β in gastric cancer interacted with NF κB and further regulates COX-2 expression. These were dissociated and downregulated by melatonin, as proven by immunofluorescence imaging, immunoprecipitation, EMSA, and Ch IP assay. Melatonin or gene silencing of C/ EBP β decreased the EMT protein markers (E-cadherin, Snail, and Slug) and Wnt/beta-catenin activity by Topflash activity, and increased ER stress markers. In an animal study, the results of melatonin therapy were consistent with those of in vitro findings and attenuated systemic proangiogenesis factor production. In conclusion, C/ EBP β and NF κB inhibition by melatonin may impede both gastric tumor growth and peritoneal dissemination by inducing ER stress and inhibiting EMT. [ABSTRACT FROM AUTHOR]

Published
2016
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93. Dual regeneration of muscle and nerve by intravenous administration of human amniotic fluid–derived mesenchymal stem cells regulated by stromal cell–derived factor-1α in a sciatic nerve injury model

Author

Yang, Dar-Yu, primary, Sheu, Meei-Ling, additional, Su, Hong-Lin, additional, Cheng, Fu-Chou, additional, Chen, Ying-Ju, additional, Chen, Chun-Jung, additional, Chiu, Wen-Ta, additional, Yiin, Jia-Jean, additional, Sheehan, Jason, additional, and Pan, Hung-Chuan, additional

Published
2012
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