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Abstract Background Exercise intervention can significantly improve physical function and bone strength; however, the effect of exercise on fall-related fractures in older adults remains controversial. This study aimed to assess the effectiveness of exercise intervention on fall-related fractures in older adults by conducting a meta-analysis of randomized controlled trials (RCTs). Methods PubMed, EMBASE, and Cochrane databases were systematically searched for RCTs through November 24, 2019 to investigate the effectiveness of exercise intervention on fall-related fractures in older adults. Pooled relative risk (RR) with 95% confidence interval (CI) was calculated using the random-effects model. Sensitivity, subgroup, and publication bias analyses were also conducted. Results A total of 7704 older adults and 428 fall-related fracture events from 20 RCTs were selected for the final meta-analysis. The follow-up duration across included trials ranged from 6.0 months to 7.0 years. The pooled RR suggested that exercise intervention was associated with a reduced fall-related fracture risk in older adults (RR: 0.74; 95% CI: 0.59–0.92; P = 0.007; I 2 = 12.6%). The pooled conclusion was robust and not affected by any individual trial. Subgroup analysis revealed that the significant effect of exercise intervention on fall-related fractures was mainly detected when the study reported results from both male and female subjects, when it did not report the baseline body mass index, when individuals received both home- and center-based interventions, when the follow-up duration was > 1.0 year, and when it was a high-quality study. Conclusions Regular exercise intervention could prevent fall-related fractures in older adults. Further large-scale RCTs should be conducted to assess the effectiveness of different exercise programs on fall-related fractures at various sites.

Abstract Aims/Introduction Elevated serum cancer antigen 19‐9 (CA19‐9) levels have been found in diabetes patients in most observational studies; however, whether there is a causal association between CA19‐9 and diabetes mellitus is unclear. Materials and Methods Our study was carried out based on the Dongfeng‐Tongji cohort comprising 27,009 individuals. We first investigated the associations between serum CA19‐9 levels and incident diabetes mellitus risk in a prospective cohort study (12,700 individuals). Then, we explored the potential causal relationship between CA19‐9 and diabetes mellitus risk in a cross‐sectional study (3,349 diabetes mellitus patients and 8,341 controls) using Mendelian randomization analysis. A weighted genetic risk score was calculated by adding the CA19‐9 increasing alleles in five single‐nucleotide polymorphisms (rs17271883, rs3760776 and rs3760775 in FUT6, rs11880333 in CA11, rs265548 in B3GNT3, and rs1047781 in FUT2), which were identified in a previous genome‐wide association study on serum CA19‐9 levels. Results In the prospective study, a total of 1,004 incident diabetes mellitus patients were diagnosed during a mean 4.54‐year follow‐up period. Elevated serum CA19‐9 level was associated with a higher incident diabetes risk after adjustment for confounders, with a hazard ratio of 1.20 (95% confidence interval 1.11–1.30) per standard deviation (12.17 U/mL) CA19‐9 increase. Using the genetic score to estimate the unconfounded effect, we did not find a causal association of CA19‐9 with diabetes risk (odds ratio per weighted CA19‐9‐increasing allele: 0.99, 95% confidence interval 0.94–1.04; P = 0.61). Conclusions The present study did not support a causal association of serum CA19‐9 with diabetes risk. CA19‐9 might be a potential biomarker of incident diabetes mellitus risk.

Abstract Background Exenatide is an insulinotropic peptide drug for type 2 diabetes treatment with low risk of hypoglycemia, and is administrated by subcutaneous injection. Oral administration is the most preferred route for lifelong treatment of diabetes, but oral delivery of peptide drug remains a significant challenge due to the absorption obstacles in gastrointestinal tract. We aimed to produce exenatide-loaded nanoparticles containing absorption enhancer, protectant and stabilizer using FDA approved inactive ingredients and easy to scale-up method, and to evaluate their long-term oral therapeutic effect in type 2 diabetes db/db mice. Results Two types of nanoparticles, named COM NPs and DIS NPs, were fabricated using anti-solvent precipitation method. In COM NPs, the exenatide was complexed with cholic acid and phosphatidylcholine to increase the exenatide loading efficiency. In both nanoparticles, zein acted as the cement and the other ingredients were embedded in zein nanoparticles by hydrophobic interaction. Casein acted as the stabilizer. The nanoparticles had excellent lyophilization, storage and re-dispersion stability. Hypromellose phthalate protected the loaded exenatide from degradation in simulated gastric fluid. Cholic acid promoted the intestinal absorption of the loaded exenatide via bile acid transporters. The exenatide loading efficiencies of COM NPs and DIS NPs were 79.7% and 53.6%, respectively. The exenatide oral pharmacological availability of COM NPs was 18.6% and DIS NPs was 13.1%. COM NPs controlled the blood glucose level of the db/db mice well and the HbA1c concentration significantly decreased to 6.8% during and after 7 weeks of once daily oral administration consecutively. Both DIS NPs and COM NPs oral groups substantially increased the insulin secretion by more than 60% and promoted the β-cell proliferation by more than 120% after the 7-week administration. Conclusions Both COM NPs and DIS NPs are promising systems for oral delivery of exenatide, and COM NPs are better in blood glucose level control than DIS NPs. Using prolamin to produce multifunctional nanoparticles for oral delivery of peptide drug by hydrophobic interaction is a simple and effective strategy.

Abstract Background Hydrogen gas (H2) is hypothesised to play a role in plants that are coping with stresses by regulating signal transduction and gene expression. Although the beneficial role of H2 in plant tolerance to cadmium (Cd) has been investigated previously, the corresponding mechanism has not been elucidated. In this report, the transcriptomes of alfalfa seedling roots under Cd and/or hydrogen-rich water (HRW) treatment were first analysed. Then, the sulfur metabolism pathways were focused on and further investigated by pharmacological and genetic approaches. Results A total of 1968 differentially expressed genes (DEGs) in alfalfa seedling roots under Cd and/or HRW treatment were identified by RNA-Seq. The DEGs were classified into many clusters, including glutathione (GSH) metabolism, oxidative stress, and ATP-binding cassette (ABC) transporters. The results validated by RT-qPCR showed that the levels of relevant genes involved in sulfur metabolism were enhanced by HRW under Cd treatment, especially the genes involved in (homo)glutathione metabolism. Additional experiments carried out with a glutathione synthesis inhibitor and Arabidopsis thaliana cad2–1 mutant plants suggested the prominent role of glutathione in HRW-induced Cd tolerance. These results were in accordance with the effects of HRW on the contents of (homo)glutathione and (homo)phytochelatins and in alleviating oxidative stress under Cd stress. In addition, the HRW-induced alleviation of Cd toxicity might also be caused by a decrease in available Cd in seedling roots, achieved through ABC transporter-mediated secretion. Conclusions Taken together, the results of our study indicate that H2 regulated the expression of genes relevant to sulfur and glutathione metabolism and enhanced glutathione metabolism which resulted in Cd tolerance by activating antioxidation and Cd chelation. These results may help to elucidate the mechanism governing H2-induced Cd tolerance in alfalfa.

Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients. To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion. The electrostatic complex of PRO and BSA–dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO). The BSA molecules were crosslinked at the oil–water interface by a heat treatment and the PRO chains were simultaneously anchored in the interface. BD emulsion (@BD) without PRO was produced for comparation. Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity. The emulsions had long-term stability. The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice. More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection. No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO. This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.

Background: Positron emission tomography – computed tomography (PET-CT) research has shown that sleep discrepancy recorded by self-report and polysomnography (PSG) may be related to the altered metabolic rate of the anterior insula (aINS) during non-rapid eye movement (NREM) sleep in patients with insomnia disorder. We aim to explore the functional connectivity of aINS across wake and NREM sleep in the patients and to reveal the association between aINS connectivity and sleep discrepancy. Methods: Patients with insomnia disorder (n = 33) and healthy controls (n = 31) underwent simultaneous electroencephalography and functional magnetic resonance imaging (EEG-fMRI) during nighttime sleep, and aINS-based connectivity was calculated across wake and NREM sleep. A linear mixed-effects model was used to assess the main effect of group and group-by-stage (wake, NREM stages 1–3) interaction effect on aINS connectivity. Similar mixed models were used to assess the potential correlation between aINS connectivity and the sleep misperception index (MI). Results: A significant group-by-stage interaction effect on aINS-based connectivity was observed in the bilateral frontal gyrus, right inferior temporal gyrus, bilateral middle occipital gyrus and right postcentral gyrus (p

Fluoride is capable of inducing developmental neurotoxicity, but the mechanisms involved remain unclear. We aimed to explore the role of autophagosome-lysosome fusion in developmental fluoride neurotoxicity, particularly focusing on the interaction between ATG14 and the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex. We developed in vivo models of Sprague–Dawley rats exposed to sodium fluoride (NaF) from the pregnancy of parental rats until the offspring were two months old and in vitro models of NaF and/or Ad-ATG14-treated SH-SY5Y cells. We assessed neurobehavioral changes in offspring and further investigated the effects of NaF exposure on autophagic flux, apoptosis, autophagosome-lysosome fusion, and the interaction between ATG14 and the SNARE complex. NaF exposure impaired offspring learning and memory capabilities and induced the accumulation of autophagosomes and autophagic flux blockage and apoptosis, as indicated by increased LC3-II, p62, and cleaved-caspase-3 expression in vivo and in vitro. In addition, NaF treatment downregulated the protein expression of ATG14 and the SNARE complex and induced autophagosome-lysosome fusion blockage as evidenced by decreased ATG14, STX17, SNAP29, and VAMP8 expression and diminished colocalization of autophagosomes and lysosomes in vivo and in vitro. Furthermore, ATG14 upregulation enhanced the interaction of ATG14 and the SNARE complex to facilitate autophagosome-lysosome fusion, thereby restoring autophagic flux and alleviating NaF-induced apoptosis. In conclusion, NaF exhibited developmental neurotoxicity by restraining the interaction of ATG14 with the SNARE complex and hindering autophagosome-lysosome fusion, thereby participating in the occurrence and development of fluoride neurotoxicity. Notably, ATG14 upregulation protects against developmental fluoride neurotoxicity, and ATG14 may serve as a promising biomarker for further epidemiological investigation.

Double pulse gas metal arc welding (DP-GMAW) is a low-frequency modulated high-frequency pulse welding, which can fully stir the molten pool, and improve the forming and performance of the weld. In this study, a mathematical model was developed using the response surface method (RSM) with three main process parameters (welding current, peak-to-base ratio, welding speed) as input values with three key geometric characteristics parameters, and the mechanical properties of the resulting welds were investigated. The results showed that the parameter model developed in this study had less error and could optimize the process parameters better; the optimal process parameters (welding current, peak-to-base ratio, welding speed) were 160 A, 39.93%, and 83.11 cm min ^−1 , respectively. The improved welding parameters result in better mechanical properties and better weld profile, and more stable welding process. As a result, this experiment provides a new perspective for process parameter optimization and mechanical properties research for weld manufacturing.

Double-wire arc additive manufacturing could manufacture large-sized components at low cost and high efficiency; however, serious heat input and heat accumulation result in poor forming quality and limit further efficiency and application. An auxiliary gas process platform for double-wire additive manufacturing is introduced in this paper to explore the influence mechanism of the auxiliary gas on deposition during the manufacturing process. Monolayer and multilayer test results showed that the auxiliary gas had a stirring effect on a liquid molten pool and a cooling effect on deposition layers, and different auxiliary gas parameters could also influence the welding arc stability, which all acted on the deposition morphology. The angle between the auxiliary gas nozzle and welding torch had the greatest impact on the formation of components, followed by the auxiliary gas flow, and the auxiliary nozzle height had minimal influence. When the angle increased from 0° to 30°, the forming morphology gradually flowed. Furthermore, increasing the auxiliary gas flow rate led to the decreased sample height and a serious flowing defect. However, decreasing the nozzle height increased the gas pressure, which would also reduce the height.

The development of effective and comprehensive methods for mapping and monitoring reservoirs is essential for the utilization of water resources and flood control. Remote sensing has the great advantages of broad spatial coverage and regular revisit to meet the demand of large-scale and long-term tasks of earth observation. Although there already exist some methods for coarse-grained identification of reservoirs at region-level in remote sensing images, it remains a challenge to recognize and localize reservoirs accurately with insufficiency of object details and samples annotated. This study focuses on the fine-grained identification and location of reservoirs with a two-stage CNN framework method, which is comprised of a coarse classification between aquatic and land areas of image patches and a fine detection of reservoirs in aquatic patches with precise geographical coordinates. Moreover, a NIR RCNN detection network is proposed to make use of the multi-spectral characteristics of Sentinel-2 images. To verify the effectiveness of our proposed method, we construct a reservoir and dam dataset of 36 Sentinel-2 images which are sampled in various provinces across China and annotated at the instance level by manual work. The experimental results in the test set show that the two-stage CNN method achieves an average recall of 80.83% nationwide, and the comparison between reservoirs recognized by the proposed model and those provided by the China Institute of Water Resources and Hydropower Research verifies that the model reaches a recall of about 90%. Both the indicator evaluation and visualization of identification results have shown the applicability of the proposed method to reservoir recognition in remote sensing images. Being the first attempt to make a fine-grained identification of reservoirs at the instance level, the two-stage CNN framework, which can automatically identify and localize reservoirs in remote sensing images precisely, shows the prospect to be a useful tool for large-scale and long-term reservoir monitoring.

Inflammatory bowel disease (IBD) is a serious hazard to public health, but the precise etiology of the disease is unclear. High intake of red meat diet is closely related to the occurrence of IBD. In this study, we investigated whether the high intake of red meat can increase the sensitivity of colitis and the underlying mechanism. Mice were fed with different levels of red meat for 8 weeks and then the colonic contents were analyzed by 16S rRNA sequencing. Then 3% dextran sulfate sodium was used to induce colitis in mice. We observed the severity of colitis and inflammatory cytokines. We found that high-dose red meat caused intestinal microbiota disorder, reduced the relative abundance of Lachnospiraceae_NK4A136_group, Faecalibaculum, Blautia and Dubosiella, and increased the relative abundance of Bacteroides and Alistipes. This in turn leads to an increase in colitis and inflammatory cytokine secretion. Moreover, we found that high red meat intake impaired the colon barrier integrity and decreased the expression of ZO-1, claudin, and occludin. We also found high red meat intake induced the production of more inflammatory cytokines such as IL-1β, TNF-α, IL-17, and IL-6 and inflammatory inducible enzymes such as COX-2 and iNOS in dextran sulfate sodium-induced colitis. These results suggest that we should optimize the diet and reduce the intake of red meat to prevent the occurrence of IBD.

Background: The root of Panax ginseng, a member of Araliaceae family, has been used as herbal medicine and functional food in Asia for thousands of years. According to Traditional Chinese medicine, ginseng is the most widely used “Qi-invigorating” herbs, which provides tonic and preventive effects by resisting oxidative stress, influencing energy metabolism, and improving mitochondrial function. Very few reports have systematically measured cell mitochondrial bioenergetics after ginseng treatment. Methods: Here, H9C2 cell line, a rat cardiomyoblast, was treated with ginseng extracts having extracted using solvents of different polarity, i.e., water, 50% ethanol, and 90% ethanol, and subsequently, the oxygen consumption rate in healthy and tert-butyl hydroperoxide–treated live cultures was determined by Seahorse extracellular flux analyzer. Results: The 90% ethanol extracts of ginseng possessed the strongest antioxidative and tonic activities to mitochondrial respiration and therefore provided the best protective effects to H9C2 cardiomyocytes. By increasing the spare respiratory capacity of stressed H9C2 cells up to three-folds of that of healthy cells, the 90% ethanol extracts of ginseng greatly improved the tolerance of myocardial cells to oxidative damage. Conclusion: These results demonstrated that the low polarity extracts of ginseng could be the best extract, as compared with others, in regulating the oxygen consumption rate of cultured cardiomyocytes during mitochondrial respiration. Keywords: Cell protection, Extracellular flux analyzer, Ginseng Radix, Live cell, Reactive oxygen species

Background: Metals exposure from natural environment and pollution have been linked to cardiovascular disease (CVD). However, whether associations existing between plasma multiple metals and incident cardiovascular disease in patients with type 2 diabetes (T2D) is unknown. Objectives: We conducted a prospective cohort study to investigate whether plasma levels of metals are associated with incident CVD risk in patients with T2D. Methods: In a prospective study of 3897 type 2 diabetes embedded in the Dongfeng–Tongji cohort, fasting blood samples were collected in 2008 at baseline and in 2013 in the first follow-up period. Plasma concentrations of 23 metals were measured by inductively coupled plasma mass spectrometry (ICP-MS). The associations between plasma metal concentrations and CVD risk in patients with T2D were investigated with Cox proportional hazards models. Results: During an average of 6.2 years follow-up, 1114 participants developed CVD. In the single-metal models adjusting for established cardiovascular risk factors, plasma zinc and selenium levels were negatively and strontium was positively associated with incident CVD risk in patients with T2D. Similar results were obtained in the multiple-metal model, the HRs (95% CIs) for zinc, selenium, and strontium comparing extreme quartiles were 0.78 (95% CI: 0.65–0.93; P trend = 0.011), 0.76 (95% CI: 0.64–0.91; P trend = 0.001), and 1.51 (95% CI: 1.26–1.81; P trend

Objective: Essential metals play important roles in the carcinogenic process. However, seldom longitudinal investigations have evaluated their roles in lung cancer development. We aimed to investigate the associations between multiple essential metals and lung cancer incidence and to explore the potential mechanisms. Methods: A nested case-control study of 440 incident lung cancer cases and 1:3 frequency matched 1320 healthy controls from the Dongfeng-Tongji Cohort was conducted. The baseline plasma concentrations of 11 essential metals (cobalt, copper, iron, manganese, molybdenum, rubidium, selenium, strontium, stannum, vanadium, and zinc) were measured, and their associations with lung cancer incidence were estimated. Effect of positive metal (zinc) on 4-year telomere attrition was then evaluated among an occupational cohort of 724 workers. We also assessed the transcriptional regulation effects of plasma zinc on mRNA expression profiles, and the expressions of zinc-related genes were further compared in pair-wised lung tumor and normal tissues. Results: Elevated plasma level of zinc was associated with lower incident risk of lung cancer [OR (95% CI) = 0.89 (0.79, 0.99)] and decreased 4-year telomere attrition [β (95% CI) = −0.73 (−1.27, −0.19)]. These effects were pronounced among males. In particularly, zinc could regulate the expressions of 8 cancer-related genes, including SOD1, APE, TP53BP1, WDR33, LAPTM4B, TRIT1, HUWE1, and ZNF813, which were over-expressed in lung tumor tissues. Conclusions: We propose that high plasma zinc could prevent incident lung cancer, probably by slowing down telomere attrition and regulating the expressions of cancer-related genes. These results provided a new insight into lung cancer prevention. Keywords: Essential metals, Zinc, Lung cancer, Cohort study, Telomere attrition, Gene expression

Introduction and aim: It is indicated that high levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) are associated with increased incident type 2 diabetes risk. However, whether serum ALT levels could improve the discrimination of type 2 diabetes remains unclear. Methods: The data was derived from the Dongfeng-Tongji cohort study, which was established in 2008 and followed until October 2013. A total of 17,173 participants free of type 2 diabetes at baseline were included and 1159 participants developed diabetes after 4.51 (0.61) years of follow-up. Cox proportional hazard regression model was used to calculate the hazard ratios (HRs) for the association between ALT and AST levels with incident diabetes risk. Receiver-operating characteristic (ROC) curves analysis was used to evaluate the predictive accuracy of models incorporating traditional risk factors with and without ALT. Results: Compared with the lowest quartile of ALT and AST levels, the highest quartile had a significantly higher risk of developing type 2 diabetes (HR: 2.17 [95% CI: 1.78–2.65] and 1.29 [1.08–1.54], respectively) after adjustment for potential confounders. The addition of ALT levels into the traditional risk factors did not improve the predictive ability of type 2 diabetes, with AUC increase from 0.772 to 0.774; P = 0.86. Conclusions: Although elevated ALT or AST levels increased incident type 2diabetes risk, addition of ALT levels into the prediction model did not improve the discrimination of type 2 diabetes.

N-3 polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), mitigate the progression of obesity-associated insulin resistance. However, the knowledge on the metabolic response and underlying mechanism of α-linolenic acid-rich linseed oil (ALA-LO) on insulin resistance was still limited. The results showed that lard-based high fat diet (HFD) feeding for 16 weeks (60% of total calories from fat) led to imbalanced lipid synthesis-oxidation, defect insulin signaling and serious mitochondrial damage in mice liver, which was attenuated by LO. Moreover, LO blocked chronic HFD-induced mitophagy suppression, and improved mitochondrial biogenesis and fusion process in mice liver. Importantly, the specific location of ALA, and its n-3 derivatives docosapentenoic acid (DPA) and DHA in mitochondrial membrane was observed, concomitant with upregulation of SIRT1, PPARγ coactivator-1α (PGC-1α) and extracellular signal regulated kinase (ERK) in mice liver. LO improves hepatic insulin resistance in obese mice partly by restoring mitochondrial quality control network.

Recently, double-wire gas metal arc welding (GMAW) process is more and more prevalently employed in industrial applications and attracted many academic researches. This paper reviewed the recent trends of researches and applications of this process. Three main aspects were included in this paper. The first was the operational process analysis of the process, and the reviewing work was divided into two catalogues: twin-wire GMAW process and tandem GMAW process, which were two main energy delivery and control formations of the process. This part focused on some negative phenomena, such as arc interruption, during the welding process and their effects on the products quality and process stability. Some works about using different current waveforms combinations and their effects on the welding process were discussed. Also, the works of numerical simulation for this process were mentioned in this part. The second part was about process stability monitoring, and corresponding works focused on establishing quantitative process stability estimation model. The last was the improvement of process control method, and the works mainly focused on the adjustment and modulation of the current waveforms, in order to eliminate negative phenomena, improve the process stability and obtain the products with high quality. Finally, some suggestions about future works were presented. This paper can provide references and enlightens for current academic researches or actual industrial applications in the double-wire GMAW process relative areas.

This study aimed to investigate the effects of quercetin that is natural functional component in food on lysosome damage-mediated autophagy dysfunction in ALD and its possible underlying mechanisms. The C57BL/6J mice were divided into four groups and pair-fed with either regular or ethanol-containing Lieber De Carli liquids diets for 15 weeks. Quercetin was received by gavage. According to the purpose of experiments, primary hepatocytes were pretreated with various pharmacological reagents. Results showed that quercetin alleviated chronic ethanol consumption induced liver injury and autophagic flux suppression. Quercetin decreased the abnormal LC3-II and p62 accumulation and increased the expression of LAMP1, LAMP2 and Rab7. Besides, quercetin reversed the inhibition of TFEB nuclear translocation incited by ethanol and exhibited similar effect to Torin 1 (mTOR activity inhibitor) which could promote TFEB nuclear translocation. Thus, regulating mTOR-TFEB pathway may be a major mechanism of quercetin for ameliorating lysosomal autophagy dysfunction induced by ethanol.

Atherosclerosis (AS) is the main pathological basis for the development of cardio-cerebrovascular diseases. Abnormal accumulation of apoptotic and necrotic cells resulted in plaque enlargement, necrotic core formation and plaque rupture in AS. Under physiological conditions, apoptotic cells (ACs) could be effectively phagocytized and cleared by phagocyte-mediated efferocytosis. In contrast, the clearance efficiency of ACs in AS plaque was much lower because of the impaired efferocytosis in AS. Recent findings have made great progress on the molecular mechanisms of efferocytosis process and dynamic regulation, and its dysfunction on organismal health. Yet, there are still few effective treatments for this process. This article reviews the mechanism of efferocytosis and the role of efferocytosis in AS, highlighting a novel therapeutic strategy for AS, which mainly prevents the progression of plaque by targeting efferocytosis.

In this paper, a double pulse gas metal arc welding (DP-GMAW) for an AA6061-T6 aluminum alloy based on fewer basic welding parameters than the traditional DP-GMAW is proposed. This study compared the difference in pulse base currents (ΔIb) and the difference in the pulse peak currents (ΔIp) by analyzing the electrical signal and morphology properties of welded samples. The results indicated that changing ΔIp caused welding defects or even welding failure easily. The welding stability after changing ΔIb was much better than that after changing ΔIp. The individual fish-scale width of the weld joint remained unchanged when ΔIb was at different values. In addition, the average absorbed work, tensile strength, yield strength and elongation of the weld joints obtained by different ΔIb values reached 31.1%, 60.2%, 52.9% and 37.9% of the base metal, respectively.

Objective To study the correlation between gastrointestinal (GI) symptoms and disease severity in patients with COVID-19.Design We searched six databases including three Chinese and three English databases for all the published articles on COVID-19. Studies were screened according to inclusion and exclusion criteria. The relevant data were extracted and all the statistical analyses were performed using Revman5.3.Result In a meta-analysis of 9 studies, comprising 3022 patients, 479 patients (13.7%, 95% CI 0.125 to 0.149) had severe disease and 624 patients (14.7%, 95% CI 0.136 to 0.159) had GI symptoms. Of 624 patients with GI symptoms, 118 patients had severe disease (20.5%, 95% CI 0.133 to 0.276) and of 2397 cases without GI symptoms, 361 patients had severe disease (18.2%, 95% CI 0.129 to 0.235). Comparing disease severity of patients with and without GI symptoms, the results indicated: I²=62%, OR=1.21, 95% CI 0.94 to 1.56, p=0.13; there was no statistically significant difference between the two groups. The funnel plot was symmetrical with no publication bias.Conclusion Current results are not sufficient to demonstrate a significant correlation between GI symptoms and disease severity in patients with COVID-19.

Abstract Background Emerging studies have investigated the association between different anthropometric indices with diabetes risk but the results were inconsistent. The aims of the study were to examine the associations of different anthropometric indices with incident diabetes risk and whether novel anthropometric indices improve diabetes prediction beyond traditional indices among elderly Chinese. Methods Nine thousand nine hundred sixty-two elderly individuals (age ≥ 60 years old) derived from the prospective Dongfeng-Tongji cohort were included. Hazard ratio (HR) and corresponding 95% confidence interval (CI) were evaluated by Cox proportional hazard model to examine the associations between traditional anthropometric indices (body mass index [BMI], waist circumference [WC], waist-to-height ratio [WHtR]), novel anthropometric indices (visceral adiposity index [VAI], a body shape index [ABSI], body roundness index [BRI]) and diabetes risk. Receiver operating characteristic (ROC) curve and area under curve (AUC) were applied to compare the novel anthropometric indices with the traditional indices in diabetes prediction. Results During mean 4.6 years of follow-up, 614 incident cases of type 2 diabetes (T2D) were identified. Significant positive associations were detected between BMI, WC, WHtR, VAI and BRI and incident T2D risk. For ABSI, no significant association was observed in either men or women. BMI was the strongest predictor in diabetes in men (AUC = 0.655) comparable with the other anthropometric indices (P

Liver is the primary acting site of insulin. In this study, we developed innovative nanoparticles for oral and liver-targeted delivery of insulin by using enterohepatic circulation of bile acids. The nanoparticles were produced from cholic acid and quaternary ammonium modified chitosan derivative and hydroxypropyl methylcellulose phthalate (HPMCP). The nanoparticles had a diameter of 239 nm, an insulin loading efficiency of 90.9%, and a loading capacity of 18.2%. Cell culture studies revealed that the cholic acid groups effectively enhanced the transport of the nanoparticles through Caco-2 cell monolayer and greatly increased the absorption of the nanoparticles in HepG-2 cells via bile acid transporter mechanism. Ex vivo fluorescence images of ileum section, gastrointestinal tract, and liver demonstrated that the HPMCP increased the mucoadhesion of the nanoparticles in ileum, and the cholic acid groups facilitated the absorptions of the nanoparticles in both ileum and liver by use of bile acid transporters via enterohepatic circulation of bile acids. The therapy for diabetic mice displayed that the oral nanoparticle group could maintain hypoglycemic effect for more than 24 h and its pharmacological availability was about 30% compared with the insulin injection group. For the first time, this study demonstrates that using enterohepatic circulation of bile acids is an effective strategy for oral delivery of insulin.

Screening a new biosorbent with low cost and high efficiency from a native plant material is a key to the adsorption of heavy metal ions from wastewater. In this study, the potential of Phytolacca americana L. as a biosorbent for Pb(II) removal from aqueous solutions was investigated. Experiments were performed to evaluate the effect of biosorbent dosage, pH, initial Pb(II) concentration and contact time on the Pb(II) removal. The results indicated that the hydroxyl, carboxyl and amine groups may take part in Pb(II) binding. HNO3-modified P. americana (HPAL) showed a significant higher uptake capacity compared to original P. americana (PAL) (p

Scar hyperplasia at the suture site is an important reason for hindering the repair effect of peripheral nerve injury anastomosis. To address this issue, two repair methods are often used. Biological agents are used to block nerve sutures and the surrounding tissue to achieve physical anti-adhesion effects. Another agent is glucocorticosteroid, which can prevent scar growth by inhibiting inflammation. However, the overall effect of promoting regeneration of the injured nerve is not satisfactory. In this regard, we envision that these two methods can be combined and lead to shared understanding for achieving improved nerve repair. In this study, the right tibial nerve was transected 1 cm above the knee to establish a rat tibial nerve injury model. The incision was directly sutured after nerve transection. The anastomotic stoma was coated with 0.5 × 0.5 cm2 chitosan sheets with betamethasone dipropionate. At 12 weeks after injury, compared with the control and poly (D, L-lactic acid) groups, chitosan-betamethasone dipropionate film slowly degraded with the shape of the membrane still intact. Further, scar hyperplasia and the degree of adhesion at anastomotic stoma were obviously reduced, while the regenerated nerve fiber structure was complete and arranged in a good order in model rats. Electrophysiological study showed enhanced compound muscle action potential. Our results confirm that chitosan-betamethasone dipropionate film can effectively prevent local scar hyperplasia after tibial nerve repair and promote nerve regeneration.

Abstract Background A sedentary lifestyle and poor diet are risk factors for the progression of non-alcoholic fatty liver disease. However, the pathogenesis of hepatic lipid accumulation is not completely understood. Therefore, the present study explored the effects of dietary supplementation of various ratios of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on a high-fat diet-induced lipid metabolism disorder and the concurrent liver damage. Methods Using high-fat diet-fed C57BL/6 J mice as the animal model, diets of various ratios of DHA/EPA (2:1, 1:1, and 1:2) with an n-6/n-3 ratio of 4:1 were prepared using fish and algae oils enriched in DHA and/or EPA and sunflower seed oils to a small extent instead of the high-fat diet. Results Significantly decreased hepatic lipid deposition, body weight, serum lipid profile, inflammatory reactions, lipid peroxidation, and expression of adipogenesis-related proteins and inflammatory factors were observed for mice that were on a diet supplemented with DHA/EPA compared to those in the high-fat control group. The DHA/EPA 1:2 group showed lower serum triglycerides (TG), total cholesterol (TC), and low-density lipoprotein-cholesterol levels, lower SREBP-1C, FAS, and ACC-1 relative mRNA expression, and higher Fra1 mRNA expression, with higher relative mRNA expression of enzymes such as AMPK, PPARα, and HSL observed in the DHA/EPA 1:1 group. Lower liver TC and TG levels and higher superoxide dismutase levels were found in the DHA/EPA 2:1 group. Nonetheless, no other notable effects were observed on the biomarkers mentioned above in the groups treated with DHA/EPA compared with the DHA group. Conclusions The results showed that supplementation with a lower DHA/EPA ratio seems to be more effective at alleviating high-fat diet-induced liver damage in mice, and a DHA/EPA ratio of 1:2 mitigated inflammatory risk factors. These effects of n-3 polyunsaturated fatty acids (PUFA) on lipid metabolism may be linked to the upregulation of Fra1 and attenuated activity of c-Jun and c-Fos, thus ultimately reducing the severity of the lipid metabolism disorder and liver damage to some extent.

Resistance spot welding (RSW) is frequently employed in current industrial occasions. This paper reviewed recent advances of the electrical structure of the RSW system. Two types of electrical structures are used in RSW machines. Single-phase ac power source has a simple structure but low control frequency and power factor, and the measurement and control for the machine have some special operations. While three-phase medium frequency dc power source has a complex structure and high control frequency but high cost, and frequently switching the direction of the output welding current may induce many negative phenomena in the welding transformer. The characteristics of the two structures are presented in detail in this paper. Corresponding measures have been taken to deal with the phenomena during the process, respectively, for the two types of electrical structures, according to the previous works. Though many significant achievements have been gained in previous works, it is expected to be more improvements required in the future. This paper can provide references and enlightens for academic researches or actual production in RSW operations.

To solve the challenge of welding aluminum alloys, a unified adjustment model for Gaussian pulse welding is established. This model can achieve improved welding performance by adjusting the base current of the weak pulse group within a specific range of average welding current inputs. The flat overlaying welding is carried out on the base material: 6061 aluminum alloys with thicknesses of 2 mm, 3 mm, and 5 mm. A stable welding process, indicated by reduced spatter, is produced, with a soft arc sound and good repeatability in the waveforms of the real-time current and voltage. The weld has a shiny surface and regular fish scale ripples. Metallographic analysis shows that the fusion line is clear, and there are no visible defects, while the weld zone has fine dendritic structures. The tensile test results indicate that fractures occur in the heat-affected zone, and that the tensile strength reaches about 68% of that of the base metal.

Wire and arc additive manufacturing (WAAM) is usually for fabricating components due to its low equipment cost, high material utilization rate and cladding efficiency. However, its applications are limited by the large heat input decided by process parameters. Here, four 50-layer stainless steel parts with double-pulse and single-pulse metal inert gas (MIG) welding modes were deposited, and the effect of different duty ratios and current modes on morphology, microstructure, and performance was analyzed. The results demonstrate that the low frequency of the double-pulse had the effect of stirring the molten pool; therefore, the double-pulse mode parts presented a bigger width and smaller height, finer microstructure and better properties than the single-pulse mode. Furthermore, increasing the duty ratio from 35% to 65% enlarged the heat input, which then decreased the specimen height, increased the width, and decreased the hardness and the tensile strength.

Aiming at existing methods unable to reveal the definition of dilution in welding accurately, such as the limited formula’s range of 0~1 mathematically, a new way which can be widely applied to various welds alloying either one or more compositions was proposed. Dilution values obtained using different methods through chemical composition analysis were compared and the effect of heat inputs on dilution was researched. The results show that the proposed method fabricated mathematically agrees well with the definition of dilution in welding. Especially, by dilution increasing to a level higher than the critical value, it reveals the fact that a weld can be concentrated as well when the filler wire is rich in the same composition as the substrate does. Besides, the method demonstrates that the gross dilution for a bead is a combined result that is affected by each individual composition. However, whether a composition is diluted or concentrated, composition’s concentrations are propelled by the increase of heat input toward that of the original substrate. During the process, the dilution value changes sharply before the heat input reaches the transition point (TP) of 0.18 kJ/mm, while once the heat input is beyond TP, the dilution becomes stable.

Serious heat accumulation limits the further efficiency and application in additive manufacturing (AM). This study accordingly proposed a double-wire SS316L stainless steel arc AM with a two-direction auxiliary gas process to research the effect of three parameters, such as auxiliary gas nozzle angle, auxiliary gas flow rate and nozzle-to-substrate distance on depositions, then based on the Box–Behnken Design response surface, a regression equation between three parameters and the total score were established to optimized parameters by an evaluation system. The results showed that samples with nozzle angle of 30° had poor morphology but good properties, and increasing gas flow or decreasing distance would enhance the airflow strength and stiffness, then strongly stir the molten pool and resist the interference. Then a diverse combination of auxiliary process parameters had different influences on the morphology and properties, and an interactive effect on the comprehensive score. Ultimately the optimal auxiliary gas process parameters were 17.4°, 25 L/min and 10.44 mm, which not only bettered the morphology, but refined the grains and improved the properties due to the stirring and cooling effect of the auxiliary gas, which provides a feasible way for quality and efficiency improvements in arc additive manufacturing.

Hepatic steatosis and insulin resistance are highly prevalent and play a vital role in the development of nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM). The present study examined the protective effects of naturally occurring flaxseed lignan secoisolariciresinol diglucoside (SDG) on these metabolic disorders resulting from high fat diet (HFD). SDG (0.05%, w/w) supplementation for 16 weeks attenuated body weight gain, hyperlipidaemia and hepatic steatosis in HFD (60% kcal from fat)-challenged C57BL/6J mice. Moreover, SDG lowered fasting serum glucose and insulin, and improved homeostasis model assessment of insulin resistance (HOMA-IR) index, glucose tolerance, and insulin response in HFD-fed mice. Furthermore, SDG prevented HFD-induced inhibition of protein kinase B, insulin receptor substrate-1 and AMP-activated protein kinase activation in the liver of HFD-fed mice. These findings indicate that SDG supplementation alleviates hepatic steatosis and insulin resistance, at least in part, by enhancing insulin signalling and AMPK activation.

Cordyceps sinensis is an endoparasitic fungus widely used as a tonic and medicinal food in the practice of traditional Chinese medicine (TCM). In historical usage, Cordyceps specifically is referring to the species of C. sinensis. However, a number of closely related species are named themselves as Cordyceps, and they are sold commonly as C. sinensis. The substitutes and adulterants of C. sinensis are often introduced either intentionally or accidentally in the herbal market, which seriously affects the therapeutic effects or even leads to life-threatening poisoning. Here, we aim to identify Cordyceps by DNA sequencing technology. Two different DNA-based approaches were compared. The internal transcribed spacer (ITS) sequences and the random amplified polymorphic DNA (RAPD)-sequence characterized amplified region (SCAR) were developed here to authenticate different species of Cordyceps. Both approaches generally enabled discrimination of C. sinensis from others. The application of the two methods, supporting each other, increases the security of identification. For better reproducibility and faster analysis, the SCAR markers derived from the RAPD results provide a new method for quick authentication of Cordyceps.

We report on the utilization of the amphiphilic poly[quaternized (2-(N,N-dimethylamino) ethyl methacrylate)]-co-(lauryl methacrylate))-b-poly[(oligo ethylene glycol) methyl ether methacrylate] QP(DMAEMA-co-LMA)-b-POEGMA cationic diblock terpolymer aggregates as nanocarriers for insulin delivery applications. QP(DMAEMA-co-LMA)-b-POEGMA random diblock terpolymer is derived from the chemical modification of the precursor amino diblock copolymer via quaternization, producing permanent positive charges on the macromolecular chain. The QP(DMAEMA-co-LMA)-b-POEGMA diblock terpolymer as well as its amino precursor investigated self-assemble in aqueous media, forming aggregates. In vitro cytotoxicity and in vivo biocompatibility studies on QP(DMAEMA-co-LMA)-b-POEGMA and its amino precursor aggregates, showed good cytocompatibility and biocompatibility. QP(DMAEMA-co-LMA)-b-POEGMA aggregates were chosen to be complexed with insulin due to their self-assembly features and the permanent positive charge in each amino group. QP(DMAEMA-co-LMA)-b-POEGMA aggregates were complexed with insulin through electrostatic interactions. Light scattering techniques were used in order to study the ability of the polymer aggregates to complex with insulin, to determine critical physicochemical parameters such as size, mass, and surface charge of the stable complexes and study the effect of salt addition on their properties. The results showed that in both cases, the complexation process was successful and as the insulin concentration increases, nanosized complexes of different physicochemical characteristics (mass, size, surface charge) and spherical morphology are formed. UV-Vis and fluorescence spectroscopy studies showed that no conformational changes of insulin occurred after the complexation.

This work is about the influence rule of inclination of welding torch on the formation and characteristics of weld bead during the pulsed-gas metal arc welding (GMAW) process based on the robotic operation. The inclination of welding torch was an important operation condition during the pulsed-GMAW process, because it can affect the formation and quality of weld bead, which was the output of the process. In this work, the different inclination modes and values were employed to conduct actual welding experiments, and some influence rules can be obtained according to examine the surface topography and cross section. Then, to obtain further rules, serious measurements for the geometry characteristic parameters were conducted and corresponding curve fitting equations between inclination angles and the bead width, penetration and bead height were obtained, and the largest error of these curve fitting equations was 0.117 mm, whose corresponding mean squared error (MSE) was 0.0103. Corresponding verification experiments validated the effectiveness of the curve fittings and showed the second order polynomials were proper, and the largest errors between measurements and curve fitting equations for inclination angle under backward mode were larger than those under forward mode, and were 0.10 mm and 0.15 mm, respectively, which corresponded to the penetration and were below 10%, therefore the equations can be used to predict the geometry of the weld bead. This work can benefit the process and operation optimization of the pulsed-GMAW process, both in the academic researches and actual industrial production.

“Yin-Yang” and “Five Elements” theories are the basis theories of Traditional Chinese Medicine (TCM). To probe and clarify the theoretical basis of these ancient Chinese wisdoms, extensive efforts have been taken, however, without a full success. In the classification of TCM herbs, hot, cold and neutral herbs are believed to possess distinct profile of chemical compositions of which the compounds should have different polarity and mass: this view provides a new perspective for further illustration. To understand the chemical properties of TCMs in the classification of “Yin-Yang” and “Five Elements,” 15 commonly used herbs attributed to spleen-meridian were selected for analyses. Chemically standardized water extracts, 50% ethanol extracts and 90% ethanol extracts were prepared and subjected to different analytic measurements. Principle component analysis (PCA) of full spectrum of HPLC, NMR and LC-MS of the extracts were established. The results revealed that the LC-MS profile showed a strong correlation with the “Yin-Yang” classification criterion. The Yang-stimulating herbs generally contain more compounds with lower molecular weight and less polar property. Additionally, a comprehensive anti-oxidative profiles of selected herbs were developed, and the results showed that its correlation with cold and hot properties of TCM, however, was rather low. Taken together, the “Yin-Yang” nature of TCM is closely related to the physical properties of the ingredients, such as polarity and molecular mass; while such classification has little correlation with anti-oxidative property. Therefore, the present results provide a new direction in probing the basic principle of TCM classification.

Chemo-resistance is an obstacle in therapy of lung cancer. Alternative therapy of using herbal medicine has been proposed to resolve this obstacle. Yu Ping Feng San (YPFS), a common Chinese herbal medicinal mixture, has been reported to show anti-drug resistance on cisplatin (DDP), a common lung cancer drug. To optimize the anti-cancer function of YPFS, different Chinese herbal extracts having known function to overcome lung cancer were screened in combining with YPFS, as to increase the efficacy of DDP in drug resistance lung cancer cell, A549/DDP. Amongst these herbal extracts, Ginkgo Folium exhibited the most promoting sensitized effect. This revised herbal formula, named as YPFS+GF, promoted the DDP-induced toxicity by over 2-fold as compared to that of YPFS alone; this potentiation was confirmed by inducing cell apoptosis. The anti-drug resistance of YPFS, triggered by an increase of intracellular concentration of DDP, was accompanied by an increased expression and activity of WT1, which consequently decreased the transcript level of MVP. In addition, the MVP-mediated downstream effector mTOR2/AKT was disrupted after application of YPFS+GF in DDP-treated A549/DDP cell: this disruption was characterized by the decline of mTORC2 components, e.g., Rictor, p-mTOR, as well as the phosphorylation level of its downstream protein AKT. The disruption on mTORC2/AKT could be reversed by mTORC2 inducer insulin and promoted by mTORC2 inhibitor PP242. Thus, the anti-drug resistance of YPFS+GF in DDP-treated lung cancer cells might be mediated by the down regulation of WT1/MVP axis, as well as the downstream anti-apoptotic pathway of mTORC2/AKT signaling. Herbal medicine is one of the main adjuvant therapies in non-small cell lung cancer, and this novel herbal formula supports the prescription of traditional Chinese medicine in cancer treatment.

Pu-erh tea is a special post-fermented tea product that contains various compounds. Quinocetone (QCT) has been used as a veterinary drug in P.R. China. However, QCT has been proven to cause oxidative DNA damage, inflammation, and apoptosis. We have used Pu-erh black tea extract (BTE) as an intervention for QCT-treated SD rats and analyzed its protective effect. Our data demonstrated that BTE improved QCT-induced functional and organic liver damage. This protective effect was accomplished by activating the Nrf2/HO-1 pathway expression and the potential mechanism consisted in the activation of the extracellular signal-regulated kinase (ERK) pathway by polyphenols contained in BTE. Moreover, some flavonoids and quinone (also contained in BTE) might effectively activate Nrf2/HO-1 pathway expression and protect SD rats from oxidative stress. Thus, the protective effect of BTE against QCT-induced oxidative damage demonstrated new insights into the antioxidative mechanisms of Pu-erh tea.

The applications of plant proteins in the food and beverage industry have been hampered by their precipitation in acidic solution. In this study, pea protein isolate (PPI) with poor dispersibility in acidic solution was used to form complexes with soybean soluble polysaccharide (SSPS), and the effects of PPI aggregates on the structure and stability of PPI/SSPS complex emulsions were investigated. Under acidic conditions, high pressure homogenization disrupts the PPI aggregates and the electrostatic attraction between PPI and SSPS facilitates the formation of dispersible PPI/SSPS complexes. The PPI/SSPS complex emulsions prepared from the PPI containing aggregates prove to possess similar droplet structure and similar stability compared with the PPI/SSPS emulsions produced from the PPI in which the aggregates have been previously removed by centrifugation. The oil droplets are protected by PPI/SSPS complex interfacial films and SSPS surfaces. The emulsions show long-term stability against pH and NaCl concentration changes. This study demonstrates that PPI aggregates can also be used to produce stable complex emulsions, which may promote the applications of plant proteins in the food and beverage industry.

Background/Aims: Curcuminoids are the main bioactive constituents of the rhizome of turmeric. Erythrocytes lesions in diabetes are probably related to hyperglycemia and protein glycation. It has been reported that curcumin prevent lipid peroxidation. However, reports on the effects of demethoxycurcumin and bis-demethoxycurcumin on human erythrocytes at high glucose levels are scarce. Our aim is to investigate the effect of curcuminoids on oxidative stress and membrane of erythrocytes exposed to hyperglycemic condition. Methods: In this study, the different blood samples were treated with two doses of glucose (10 or 30 mM) to mimic hyperglycemia in the presence or absence of three kinds of curcuminoids (5 or 10 μM) in a medium at 37 °C for 24 h (Each experiment consists of 20 blood samples from 10 male and 10 female volunteers). The malondialdehyde was checked by HPLC, antioxidase (GSH and GSSG) were measured by LC/MS, SOD was checked by WST-1 kit, morphology and phospholipid symmetry were detected by flow cytometry, confocal scanning microscope and scanning electron microscope. Results: The results illustrated that all three curcuminoids reduce oxidative stress damage on the membrane and maintain a better profile for erythrocytes. Furthermore, three curcuminoids had benefit effects on antioxidase. Conclusion: The three kinds of curcuminoids supplementation may prevent lipid peroxidation at different intensity and membrane dysfunction of human erythrocytes in hyperglycemia.

Whether cigarette smoking and moderate drinking are associated with non-alcoholic fatty liver disease (NAFLD)has not been fully described. This study investigated the separate and joint effects of smoking and moderate drinking on Chinese men with NAFLD.Across-sectional assay from DFTJ Cohort study was performed with a size of 9432 elderly Chinese men excluding excessive alcohol consumption (

Acori Tatarinowii Rhizome (ATR; the dried rhizome of Acori tatarinowii Schott) is a well-known herb being used for mental disorder in China and Asia. Volatile oil is considered as the active ingredient of ATR, and asarones account for more than 90% of total volatile oil. Here, the protective effects of ATR oil and asarones, both α-asarone and β-asarone, were probed in cultured rat astrocytes. The cyto-protective effect of ATR oil and asarones against tBHP-induced astrocyte injury was revealed, and additionally ATR oil and asarones reduced the tBHP-induced intracellular reactive oxygen species (ROS) accumulation. In parallel, the activity of anti-oxidant response element (ARE) promoter construct (pARE-Luc), being transfected in cultured astrocytes, was markedly induced by application of ATR oil and asarones. The mRNAs encoding anti-oxidant enzymes, e.g. glutathione S-transferase (GST), glutamate-cysteine ligase modulatory subunit (GCLM), glutamate-cysteine ligase catalytic subunit (GCLC) and NAD(P)H quinone oxidoreductase (NQO1) were induced by ATR oil and asarones in a dose-dependent manner. The ATR oil/asarone-induced gene expression could be mediated by Akt phosphorylation; because the applied LY294002, a phosphoinositide 3-kinase inhibitor, fully abolished the induction. These results demonstrated that α-asarone and β-asarone could account, at least partly, the function of ATR being a Chinese medicinal herb.

OBJECTIVES:The aim of this study is to examine the bidirectional association between nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM). METHODS:The data was derived from the Dongfeng-Tongji cohort study, which was established in 2008 and followed until October 2013. NAFLD was classified as none, mild, moderate/severe based on ultrasound examination. The analysis to examine the association between NAFLD and incident T2DM risk included 18,111 participants free of diabetes at baseline and the duration of follow-up was 4.60 ± 0.60 years. Cox proportional regression model was used to calculate the hazard ratio (HR) for the association. The analysis to investigate the association between T2DM and incident NAFLD risk included 12,435 participants free of NAFLD at baseline. Logistic regression model was used to calculate the odd ratio (OR) of NAFLD. RESULTS:Compared with those without NAFLD, individuals with mild or moderate/severe NAFLD had a monotonic elevated risk of developing T2DM (HR: 1.88 [95% CI: 1.63-2.18] and 2.34 [1.85-2.96], respectively) after adjustment for potential confounders. In a parallel analysis, compared to participants with fasting plasma glucose < 6.1 mmol/L, the ORs of developing NAFLD in subjects with impaired fasting glucose and T2DM were 1.35 (95% CI: 1.16-1.57) and 1.40 (95% CI: 1.22-1.62), respectively. CONCLUSIONS:Our results provide compelling evidence that the NAFLD-T2DM association is bidirectional in Chinese population.

Worldwide, there is an unintended pregnancy rate of 40%. That rate is higher in some areas, such as in the United States—where it is nearly 50%. These pregnancies have potentially negative outcomes for the mother and child that include health, economic, social, and psychological aspects. Long-acting reversible contraceptives (LARCs) are highly reliable methods for family planning. Identifying personal characteristics associated with choosing LARCs will better address the unintended pregnancy rates. The purpose of this research project was to evaluate characteristics of adolescents and women at high risk for unintended pregnancies by (a) describing the use of a LARC and selected personal characteristics for the years 2009 and 2014 and (b) exploring relationships among LARC choice and selected personal characteristics of clients seeking family planning services. A retrospective chart review was conducted on 268 randomly selected records, half of which were each from the years 2009 and 2014. One Midwestern U.S. family planning clinic was the site of the research. The characteristics used in the chart reviews were derived from the literature and Healthy People 2020 goals. Key differences between the years 2009 and 2014 included a significant increase in the number of LARCs prescribed to high-risk women. There was a significant relationship between the choice of a LARC and having used one in the past. Personal characteristics influencing LARC birth control choice included White, Hispanic females, those with a history of a teen pregnancy, those who experienced any previous pregnancy or live birth, and those below the poverty level. The findings indicate the importance of educating clients on LARC options. To foster initial use of a LARC, an application, based on the findings of this study, has been developed that provides individualized choices and education on contraceptive methods including LARCs.

Weld bead forming rule is very important during double-pulsed gas metal arc welding (DP-GMAW) process, and this process has more advantages than that of conventional arc welding process. This work employed grey rational analysis to explore the weld bead forming rule. Since the latest twinpulse XT DP control process was employed, the parameters adjustment was easier than that of conventional operation. The grey relational analyses between five main process parameters, which were average welding current, welding speed, twin pulse relation, twin pulse frequency together with twin pulse current change in percent, and three key characteristic parameters, which were bead width, bead height and penetration, were conducted to explore the weld bead forming rule. To accurately calculate the grey relational degree, the negative relevancies were transformed to positive ones. According to calculations and corresponding analyses, it can be concluded that the effects of average welding current and welding speed on the weld bead forming and key characteristic parameters of the weld bead were higher than that of other process parameters. Moreover, the relevancies between key characteristic parameters of the weld bead, and process parameters which included twin pulse relation, average welding current and twin pulse current change in percent were positive, while the relevancies between key characteristic parameters and other two process parameters were negative. The work can supply a new method to evaluate the effects of process parameters during the DP-GMAW process on the weld bead forming or other process characteristics, and references for parameters selection and process optimization.

The double pulsed gas metal arc welding (DP-GMAW) process has been effectively employed to realize joining of steel plates and obtain weld bead surfaces with high quality fish scale ripples. In this work, a DP-GMAW process based on robot operation using the latest twinpulse XT DP control technology was employed to join the stainless-steel base plates. Four key operational parameters, which were robot welding speed, twin pulse frequency, twin pulse relation and twin pulse current change in percent, were selected to be input elements of orthogonal experimental design, which included nine experiments with three levels. To accurately understand the performance and process of weld bead obtained from DP-GMAW operation based on robot operation, the appearance observation and key shape parameters measurement, microstructure analysis, tensile and hardness testing, as well as stability analysis of the electrical signals, were conducted. Correlation analysis showed that the grain size was significantly correlative to the toughness and hardness. Then, to obtain quantitative evaluation results, fuzzy comprehensive evaluation (FCE) was employed to provide quality evaluation of weld beads from the above experiments. The influential levels of the key operational parameters on the appearance, grain size and FCE scores, and corresponding physical analyses, were respectively presented. In addition, optimal parameters combinations for obtaining weld beads with optimal appearance, grain size, and the highest FCE scores of weld bead quality were respectively provided according to the range analysis of the results from orthogonal experimental design. This work can provide an effective analysis method of influential levels of key operational parameters on the performance of the weld bead, optimal operational parameters combination seeking method, and quantitative quality evaluation method for the DP-GMAW process, which can improve the process optimization and increase the production efficiency, both in academic research and actual industrial production.

To reduce weight and improve overall performance of the industrial welding robot, light-weight design using the finite element method and structural topological optimization is presented in this paper. The work analyzed an upper arm of an industrial welding robot in the most unfavorable working condition, both under static and dynamic working situations, using ABAQUS and ADAMS software tools. Then the Tosca unit in ABAQUS was employed to accomplish the structural topological optimization, in order to reduce weight and improve the natural frequencies under the situation of low orders. The analyses results showed that the actual weight had been reduced to 17.9%, and the natural frequencies in low orders had increased. The maximum Mises stress, tensile stress, and elastic displacement of the gyration center had decreased. Lastly, an actual product was produced according to the model obtained from preceding analyses. The experiments of the repeatability tests showed that the overall performance of the optimized upper arm had been improved when compared to the original one. This research can present references and foundations for the kinetic analyses under the static and dynamic working conditions, and structural topological optimization designs for relative industrial welding robots.

This study focuses on the characteristics of the ripples of the weld bead formed during the double-pulsed gas metal arc welding (DP-GMAW) process. As a special output of the process, ripples include many useful information and can reflect the quality of the welding process. The work analyzed the operational characteristics of the DP-GMAW process based on robot operation which used the latest twinpulse XT DP control process, and then selected five key operational parameters, such as average current, welding speed, twin pulse frequency, twin pulse relation, and twin pulse current change in percent, to explore their effects on the formation and characteristics of ripples. A reliable method of measuring the distance of the ripples was used to provide convincing data. According to a series of experimental observations and analyses, the distance of ripples and appearance under different conditions were obtained. Also, curve fitting equations between each operational parameter and corresponding distances of ripples was obtained. To testify the effectiveness of the curve fitting equations, corresponding verifying experiments were conducted, and the results showed that all the errors were below 10%. In addition, the different levels of the operational parameters on the formation and characteristics of ripples were provided. This work can be a reference for the process and quality control improvement for the DP-GMAW process.