81 results on '"Ricci, Serena"'
Search Results
52. Circulating tumor cells in diagnosing lung cancer: clinical and morphologic analysis
- Author
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Antonia, Feola, Adelaide, Auletta, Letizia, Cito, Rosamaria, Iorio, Alfonso, Fiorelli, Matteo, Pierdiluca, Ricci, Serena, DI CARLO, Angelina, and Marina Di Domenico
- Subjects
lung cancer ,Circulating tumor cells - Published
- 2016
53. Matrix Metalloproteinasi-9: emergente biomarcatore nei meningiomi?
- Author
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Ricci, Serena, Dario, Bruzzese, Elia, Guadagno, Marialaura Del Basso De Caro, Carmela, Peca, Sgulò, Francesco G., Francesco, Maiuri, and DI CARLO, Angelina
- Published
- 2016
54. Secreted miR-210-3p as non-invasive biomarker in clear cell renal cell carcinoma
- Author
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Petrozza, Vincenzo, primary, Pastore, Antonio Luigi, additional, Palleschi, Giovanni, additional, Tito, Claudia, additional, Porta, Natale, additional, Ricci, Serena, additional, Marigliano, Chiara, additional, Costantini, Manuela, additional, Simone, Giuseppe, additional, Di Carlo, Angelina, additional, Gallucci, Michele, additional, Carbone, Antonio, additional, and Fazi, Francesco, additional
- Published
- 2017
- Full Text
- View/download PDF
55. A peptide antagonist of Prep1-p160 interaction improves ceramide-induced insulin resistance in skeletal muscle cells
- Author
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Cimmino, Ilaria, primary, Lorenzo, Virginia, additional, Fiory, Francesca, additional, Doti, Nunzianna, additional, Ricci, Serena, additional, Cabaro, Serena, additional, Liotti, Antonietta, additional, Vitagliano, Luigi, additional, Longo, Michele, additional, Miele, Claudia, additional, Formisano, Pietro, additional, Beguinot, Francesco, additional, Ruvo, Menotti, additional, and Oriente, Francesco, additional
- Published
- 2017
- Full Text
- View/download PDF
56. Beta Oscillatory Changes and Retention of Motor Skills during Practice in Healthy Subjects and in Patients with Parkinson's Disease
- Author
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Nelson, Aaron B., primary, Moisello, Clara, additional, Lin, Jing, additional, Panday, Priya, additional, Ricci, Serena, additional, Canessa, Andrea, additional, Di Rocco, Alessandro, additional, Quartarone, Angelo, additional, Frazzitta, Giuseppe, additional, Isaias, Ioannis U., additional, Tononi, Giulio, additional, Cirelli, Chiara, additional, and Ghilardi, M. Felice, additional
- Published
- 2017
- Full Text
- View/download PDF
57. SEEG assistant: a 3DSlicer extension to support epilepsy surgery
- Author
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Narizzano, Massimo, primary, Arnulfo, Gabriele, additional, Ricci, Serena, additional, Toselli, Benedetta, additional, Tisdall, Martin, additional, Canessa, Andrea, additional, Fato, Marco Massimo, additional, and Cardinale, Francesco, additional
- Published
- 2017
- Full Text
- View/download PDF
58. Evaluation of matrix metalloproteinase type IV-collagenases in serum of patients with tumors of the central nervous system
- Author
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Ricci, Serena, primary, Guadagno, Elia, additional, Bruzzese, Dario, additional, Del Basso De Caro, Marialaura, additional, Peca, Carmela, additional, Sgulò, Francesco G., additional, Maiuri, Francesco, additional, and Di Carlo, Angelina, additional
- Published
- 2016
- Full Text
- View/download PDF
59. Serum levels of gelatinases, their inhibitors and neutrophil gelatinase-associated lipocalin: worthwhile non-invasive biomarkers for bladder cancer patients' managment
- Author
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Ricci, Serena, Bruzzese, Dario, and DI CARLO, Angelina
- Subjects
TIMPs ,Bladder cancer ,MMPs ,NGAL - Published
- 2015
60. Malvidin's effects on blood brain barrier alterations during hypoperfusion/ reperfusion injury
- Author
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Lapi, D, Mastantuono, T, Chiaruzzi, M, Battiloro, L, Di Maro, M, Ricci, Serena, DI CARLO, Angelina, and Colantuoni, A
- Subjects
pial microcirculation ,reperfusion ,metalloproteinases - Published
- 2015
61. Different forms of sera gelatinases in human intracranial neoplasias
- Author
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Ricci, Serena, Bruzzese, Dario, Guadagno, Elia, Peca, Carmela, Sgulò, Francesco G., Del Basso De Caro, Marialaura, Maiuri, Francesco, and DI CARLO, Angelina
- Published
- 2015
62. Evaluation of matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinse-2 (MMP-2) in sera of intracranial tumor patients
- Author
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Elia, Guadagno, Marialaura Del Basso De Caro, Dario, Bruzzese, Ricci, Serena, Carmela, Peca, Sgulò, Francesco G., Francesco, Maiuri, and DI CARLO, Angelina
- Subjects
Matrix metalloproteinases ,zymography ,intracranial tumors ,sera - Published
- 2015
63. Multifaceted Breast Cancer: The Molecular Connection With Obesity
- Author
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Feola, Antonia, primary, Ricci, Serena, additional, Kouidhi, Soumaya, additional, Rizzo, Antonietta, additional, Penon, Antonella, additional, Formisano, Pietro, additional, Giordano, Antonio, additional, Di Carlo, Angelina, additional, and Di Domenico, Marina, additional
- Published
- 2016
- Full Text
- View/download PDF
64. Malvidin’s Effects on Rat Pial Microvascular Permeability Changes Due to Hypoperfusion and Reperfusion Injury
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Lapi, Dominga, primary, Chiurazzi, Martina, additional, Di Maro, Martina, additional, Mastantuono, Teresa, additional, Battiloro, Laura, additional, Sabatino, Lina, additional, Ricci, Serena, additional, Di Carlo, Angelina, additional, Starita, Noemy, additional, Guida, Bruna, additional, Santillo, Mariarosaria, additional, and Colantuoni, Antonio, additional
- Published
- 2016
- Full Text
- View/download PDF
65. Evaluation of MMP-2, MMP-9, TIMP-1, TIMP-2, NGAL and MMP-9/NGAL complex in urine and sera from patients with bladder cancer
- Author
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RICCI, SERENA, primary, BRUZZESE, DARIO, additional, and DI CARLO, ANGELINA, additional
- Published
- 2015
- Full Text
- View/download PDF
66. Substrate-zymography: a still worthwhile method for gelatinases analysis in biological samples
- Author
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Ricci, Serena, primary, D’Esposito, Vittoria, additional, Oriente, Francesco, additional, Formisano, Pietro, additional, and Di Carlo, Angelina, additional
- Published
- 2015
- Full Text
- View/download PDF
67. Substrate-zymography: a still worthwhile method for gelatinases analysis in biological samples.
- Author
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Ricci, Serena, D'Esposito, Vittoria, Oriente, Francesco, Formisano, Pietro, and Di Carlo, Angelina
- Subjects
- *
MATRIX metalloproteinases , *ENDOPEPTIDASES , *GELATINASES , *CANCER diagnosis , *CANCER treatment - Abstract
Matrix metallo-proteinases (MMPs) are a family of zinc-dependent endopeptidases, capable of degrading all the molecular components of extracellular matrix. A class of MMPs is gelatinases which includes gelatinase A or MMP-2 (72 kDa) and gelatinase B or MMP-9 (92 kDa), which have been shown to play critical roles in pathophysiology of many human disease and, in particular, cancer progression. For these reasons they obtained a great interest as potential non-invasive biomarker in providing useful clinical information in cancer diagnosis and therapy. A sensitive and unexpensive method for analysis of gelatinases is the gelatine zymography, which allows to measure the relative amounts of active and inactive enzymes in body fluids and tissue extracts. The procedure involves the electrophoretic separation of proteins under denaturing but non reducing conditions through a polyacrylamide gel containing a synthetic substrate (gelatin). The aim of this mini-review has been to describe the general principles of gelatine zymography technique, underling the main advantages and disadvantages. Even though an improvement of this method is necessary for a better applicability in laboratory medicine, gelatine zymography represents the most convenient method to detect the activity of the different gelatinases from a wide range of biological samples. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
68. Matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in diagnosis of pleural effusion of malignant origin.
- Author
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Fiorelli, Alfonso, Ricci, Serena, Feola, Antonia, Mazzella, Antonio, D'Angelo, Luigi, Santini, Mario, Di Domenico, Marina, and Di Carlo, Angelina
- Published
- 2016
- Full Text
- View/download PDF
69. Prep1 Deficiency Affects Olfactory Perception and Feeding Behavior by Impairing BDNF-TrkB Mediated Neurotrophic Signaling
- Author
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Serena Cabaro, Davide Viggiano, Francesco Oriente, Francesco Beguinot, Francesca Fiory, Ilaria Cimmino, Rosa Spinelli, Serena Ricci, Antonietta Liotti, Giuseppe Perruolo, Angelina Di Carlo, Pietro Formisano, Ricci, Serena, Viggiano, Davide, Cimmino, Ilaria, Perruolo, Giuseppe, Cabaro, Serena, Liotti, Antonietta, Fiory, Francesca, Spinelli, Rosa, Di Carlo, Angelina, Beguinot, Francesco, Formisano, Pietro, and Oriente, Francesco
- Subjects
0301 basic medicine ,BDNF ,Calbindin ,Olfactory bulb ,Prep1 ,TrkB ,Cellular and Molecular Neuroscience ,Neuroscience (miscellaneous) ,Hindbrain ,Tropomyosin receptor kinase B ,Biology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Mediator ,Cell Line, Tumor ,Animals ,Receptor, trkB ,Viability assay ,Transcription factor ,Homeodomain Proteins ,Neurons ,Behavior, Animal ,Staining and Labeling ,Brain-Derived Neurotrophic Factor ,olfactory bulb ,neuroscience (miscellaneous) ,neurology ,cellular and molecular neuroscience ,Brain ,Feeding Behavior ,Olfactory Perception ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,nervous system ,Neurology ,biology.protein ,030217 neurology & neurosurgery ,Signal Transduction ,Neurotrophin - Abstract
Prep1 is a homeodomain transcription factor which has an important role in hindbrain development. Prep1 expression is also kept in adult mouse brain and in particular within the olfactory bulbs. Moreover, many Prep1 neurons co-localize with Calbindin-positive periglomerular interneurons in olfactory glomerular layer. However, Prep1 function in this brain region is still unknown. In this study, we show that Prep1 hypomorphic heterozygous (Prep1i/+) mice express low levels of protein and feature a 30% reduction of olfactory bulb area, compared to WT mice. In addition, Prep1i/+ mice olfactory bulb histological analysis indicated a 20% lower cytochrome C oxidase activity within the glomerular layer, accompanied by a reduced number of periglomerular interneurons, compared to the WT littermates. Consistently, olfactory perception test highlighted that Prep1 hypomorphic heterozygous mice display a scant ability to distinguish odors, which significantly impacts on feeding behavior, as Prep1i/+ mice revealed a reduced preference for high-fat food. Analysis of BDNF signaling, which represents the main molecular mediator of olfactory plasticity, showed that Prep1i/+ mouse olfactory bulbs feature a 30% reduction of TrkB receptor levels and a decreased activation of ERK1/2. Similarly, overexpression of Prep1 in mouse neuronal cells (N2A) caused an increase of TrkB expression levels, BDNF-induced ERK phosphorylation, and cell viability, compared to control cells. We conclude that Prep1 deficiency alters olfactory morpho-functional integrity and olfaction-mediated eating behavior by affecting BDNF-TrkB signaling. Prep1 could, therefore, play a crucial role in behavioral dysfunctions associated to impaired responsiveness to BDNF. Electronic supplementary material The online version of this article (10.1007/s12035-018-0873-7) contains supplementary material, which is available to authorized users.
- Published
- 2018
70. Evaluation of matrix metalloproteinase type IV-collagenases in serum of patients with tumors of the central nervous system
- Author
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Francesco Maiuri, Francesco Giovanni Sgulò, C. Peca, Elia Guadagno, Marialaura Del Basso De Caro, Dario Bruzzese, Angelina Di Carlo, Serena Ricci, Ricci, Serena, Guadagno, Elia, Bruzzese, Dario, DEL BASSO DE CARO, Marialaura, Peca, Carmela, Sgulo', FRANCESCO GIOVANNI, Maiuri, Francesco, and DI CARLO, Angelina
- Subjects
Adult ,Male ,Serum ,Cancer Research ,Pathology ,medicine.medical_specialty ,Adolescent ,Angiogenesis ,Population ,Clinical Neurology ,Biology ,Matrix metalloproteinase ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Vasculogenesis ,Biomarkers, Tumor ,medicine ,Humans ,Zymography ,education ,Aged ,Tumors ,Aged, 80 and over ,Basement membrane ,education.field_of_study ,Tumor ,Brain Neoplasms ,Middle Aged ,medicine.disease ,Matrix metalloproteinases ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,Neurology ,Oncology ,Central nervous system ,030220 oncology & carcinogenesis ,Laboratory Investigation ,Matrix Metalloproteinase 2 ,Immunohistochemistry ,Female ,Neurology (clinical) ,central nervous system ,matrix metalloproteinases ,serum ,tumors ,030217 neurology & neurosurgery - Abstract
The basement membrane collagen IV-degrading matrix metalloproteinases -2 and -9 (MMPs) are most often linked to the malignant phenotype of tumor cells by playing a critical role in invasion, metastasis, angiogenesis, and vasculogenesis. We verified the activity of these two MMPs in the sera of patients affected by brain tumors (20 gliomas, 28 meningiomas and 20 metastasis) by zymography. The sera of 25 healthy volunteers with no concomitant illnesses were used for controls. Zymography showed four dominant gelatinolytic bands of 240, 130, 92 (MMP-9) and 72 (MMP-2) kDa. No statistically significant variations of MMP-2 proteolytic activity between patients and healthy individuals were observed. On the contrary, MMP-9 (both monomeric and multimeric forms) lytic activities were significantly higher in tumors specimens compared to healthy controls (p < 0.001). Moreover, MMP-9 immunohistochemistry revealed: (1) a strong reactivity in neoplastic vessels of high-grade gliomas showing an inverse correlation with serum multimeric gelatinolytic activity; (2) a cytoplasmatic reactivity in meningiomas with a significantly increase in atypical meningioma compared with low-grade ones (p = 0.036); (3) a positive correlation between MMP-9 and Ki-67 (Sperman Rho coefficient r = 0.418 and p = 0.034). Our results suggest that serum and tissue MMP-9 might provide clinicians additional objective information in intracranial neoplasms. Finally, it should be possible to use MMP-9 as a target for new forms of therapy. Nevertheless, due to the small number of patients included in the study, the conclusion may not be transferable to the general population and therefore further evaluations are needed.
- Published
- 2016
71. The enigmatic role of matrix metalloproteinases in epithelial-to-mesenchymal transition of oral squamous cell carcinoma: Implications and nutraceutical aspects
- Author
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Federica Pinto, Adelaide Auletta, Alfonso Giovane, Serena Ricci, Mariarosaria Boccellino, Angelina Di Carlo, Marina Di Domenico, Giuliana Settembre, Silvia Boffo, Antonio Giordano, Ricci, Serena, Pinto, Federica, Auletta, Adelaide, Giordano, Antonio, Giovane, Alfonso, Settembre, Giuliana, Boccellino, Mariarosaria, Boffo, Silvia, Di Carlo, Angelina, and Di Domenico, Marina
- Subjects
0301 basic medicine ,curcumin ,epithelial-to-mesenchymal transition (EMT) ,matrix metalloproteinases-9 (MMP-9) ,oral squamous cell carcinoma (OSCC) ,resveratrol ,Matrix metalloproteinase ,Resveratrol ,Malignancy ,medicine.disease_cause ,Biochemistry ,Extracellular matrix ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,epithelial‐to‐mesenchymal transition (EMT) ,medicine ,Epithelial–mesenchymal transition ,matrix metalloproteinases‐9 (MMP‐9) ,Molecular Biology ,business.industry ,Cancer ,Cell Biology ,medicine.disease ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Carcinogenesis ,business - Abstract
The most prevalent malignancy in the oral cavity is represented by oral squamous cell carcinoma, an aggressive disease mostly detected in low-income communities. This neoplasia is mostly diffused in older men particularly exposed to risk factors such as tobacco, alcohol, and a diet rich in fatty foods and poor in vegetables. In oral squamous cell carcinoma, a wide range of matrix-cleaving proteinases are involved in extracellular matrix remodeling of cancer microenvironment. In particular, matrix metalloproteinases (MMPs) represent the major and most investigated protagonists. Owing to their strong involvement in malignant pathologies, MMPs are considered the most promising new biomarkers in cancer diagnosis and prognosis. The interest in studying MMPs in oral cancer biology is also owing to their prominent role in epithelial-to-mesenchymal transition (EMT). EMT is an intricate process involving different complex pathways. EMT-related proteins are attractive diagnostic biomarkers that characterize the activation of biological events that promote cancer's aggressive expansion. Different antioncogenic natural compounds have been investigated to counteract oral carcinogenesis, with the scope of obtaining better clinical results and lower morbidity. In particular, we describe the role of different nutraceuticals used for the regulation of MMP-related invasion and proliferation of oral cancer cells.
- Published
- 2019
72. Malvidin's Effects on Rat Pial Microvascular Permeability Changes Due to Hypoperfusion and Reperfusion Injury
- Author
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Dominga Lapi, Laura Battiloro, Noemy Starita, Teresa Mastantuono, Serena Ricci, Mariarosaria Santillo, Antonio Colantuoni, Martina Chiurazzi, Bruna Guida, Angelina Di Carlo, Martina Di Maro, Lina Sabatino, Lapi, Dominga, Chiurazzi, Martina, DI MARO, Martina, Mastantuono, Teresa, Battiloro, Laura, Sabatino, Lina, Ricci, Serena, Di Carlo, Angelina, Starita, Noemy, Guida, Bruna, Santillo, Mariarosaria, and Colantuoni, Antonio
- Subjects
medicine.medical_specialty ,Vascular permeability ,030204 cardiovascular system & hematology ,metalloproteinases ,03 medical and health sciences ,chemistry.chemical_compound ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Enos ,Internal medicine ,medicine ,Zymography ,radical oxygen species ,Metalloproteinase ,Original Research ,pial microcirculation ,endothelial nitric oxide synthase ,bilateral common carotid artery occlusion ,malvidin ,reperfusion ,zymography ,biology ,Chemistry ,Anatomy ,medicine.disease ,biology.organism_classification ,Malvidin ,cellular and molecular neuroscience ,Nitric oxide synthase ,Red blood cell ,medicine.anatomical_structure ,Endocrinology ,biology.protein ,Radical oxygen specie ,Reperfusion injury ,Perfusion ,030217 neurology & neurosurgery ,Neuroscience - Abstract
The present study was aimed to evaluate the malvidin’s protective effects on damage induced by 30 min bilateral common carotid artery occlusion (BCCAO) and 60 min reperfusion in rat pial microcirculation. Rat pial microcirculation was observed using fluorescence microscopy through a closed cranial window. Western blotting analysis was performed to investigate the endothelial nitric oxide synthase (eNOS), phosphorylated eNOS (p-eNOS) and matrix metalloproteinase 9 (MMP-9) expression. Moreover, MMP-9 activity was evaluated by zymography. Finally, neuronal damage and radical oxygen species (ROS) formation were assessed. In all animals, pial arterioles were classified in five orders of branching according to Strahler’s method. In hypoperfused rats, 30 min BCCAO and 60 min reperfusion caused a decrease in arteriolar diameter, an increase in microvascular leakage and leukocyte adhesion, accompanied by decreased capillary perfusion and red blood cell velocity (VRBC). Moreover, marked neuronal damage and evident ROS generation were detected. Conversely, malvidin administration induced arteriolar dilation in dose-related manner, reducing microvascular leakage as well as leukocyte adhesion. Capillary perfusion and VRBC were protected. Nitric oxide synthase inhibition significantly attenuated malvidin’s effects on arteriolar diameter. Western blotting analysis revealed an increase in eNOS and p-eNOS expression, while zymography indicated a decrease in MMP-9 activity after malvidin’s administration. Furthermore, malvidin was able to prevent neuronal damage and to decrease ROS generation. In conclusion, malvidin protects rat pial microcirculation against BCCAO/reperfusion injury, preventing blood-brain impairment and neuronal loss. Malvidin’s effects appear to be mediated by eNOS activation and scavenger activity.
- Published
- 2016
73. Matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in diagnosis of pleural effusion of malignant origin
- Author
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Alfonso Fiorelli, Marina Di Domenico, Angelina Di Carlo, Serena Ricci, Antonia Feola, Antonio Mazzella, Luigi D'Angelo, Mario Santini, Fiorelli, A., Ricci, S., Feola, A., Mazzella, A., D'Angelo, L., Santini, M., Di Domenico, M., Di Carlo, A., Fiorelli, Alfonso, Ricci, Serena, Feola, Antonia, Mazzella, Antonio, D'Angelo, Luigi, Santini, Mario, DI DOMENICO, Marina, and Di Carlo, Angelina
- Subjects
Pulmonary and Respiratory Medicine ,Male ,Pathology ,medicine.medical_specialty ,Exudative pleural effusion ,Pleural effusion ,Reproducibility of Result ,Enzyme-Linked Immunosorbent Assay ,Predictive Value of Test ,030204 cardiovascular system & hematology ,Diagnosis, Differential ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Carcinoembryonic antigen ,Predictive Value of Tests ,Lactate dehydrogenase ,Cytology ,medicine ,Biomarkers, Tumor ,Humans ,Zymography ,Aged ,Tissue Inhibitor of Metalloproteinase-1 ,biology ,business.industry ,Reproducibility of Results ,matrix metalloproteinase-9 ,tissue inhibitor of metalloproteinase-1 ,exudative pleural effusion ,Histology ,Matrix metalloproteinase-9 ,Tissue inhibitor of metalloproteinase ,Middle Aged ,medicine.disease ,Pleural Effusion, Malignant ,chemistry ,Italy ,Matrix Metalloproteinase 9 ,ROC Curve ,030220 oncology & carcinogenesis ,Area Under Curve ,biology.protein ,Surgery ,Female ,Cardiology and Cardiovascular Medicine ,business ,Human - Abstract
OBJECTIVES The aim of the present study was to evaluate the diagnostic accuracy of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in differentiating benign from malignant exudative pleural effusions. METHODS This is a unicentre observational study including 97 consecutive patients with exudative pleural effusions. Metalloproteinase-9, tissue inhibitor of metalloproteinase-1, lactate dehydrogenase, ferritin, carcinoembryonic antigen and carbohydrate antigen 15-3 were measured in pleural effusion and serum by enzyme-linked immunosorbent assay. The activity of metalloproteinase-9 was also evaluated by substrate zymography. The data were correlated with final diagnosis of pleural effusions to evaluate the diagnostic accuracy. RESULTS Of the 97 eligible patients, 6 were excluded. Of the 91 patients included in the study, 70 had malignant pleural effusions and 21 had benign pleural effusions. Both in sera and pleural effusions, matrix metalloproteinase-9 (P < 0.0001), tissue inhibitor of metalloproteinase-1 (P < 0.0001) and carcinoembryonic antigen (P < 0.0001) levels were higher in neoplastic patients than in benign group. Zymography analysis showed a most prominent band at a molecular weight of 92 kDa (metalloproteinase-9) whereas a less intense band was observed at 72 kDa (metalloproteinase-2). A significant correlation was found between metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 levels in pleural effusion (P < 0.0001; r = 0.8) and serum (P < 0.03; r = 0.2). Pleural effusion metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 levels showed higher value of sensitivity (97 and 91%, respectively) and specificity (90 and 95%, respectively) compared with other standard markers. Serum metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 levels showed similar results. Among 70 neoplastic patients, 29 had negative pleural cytology. Of these, 25 presented elevated levels of metalloproteinase-9 and tissue inhibitor of metalloproteinase-1, whereas 4 patients had elevated levels of one of the two markers. CONCLUSIONS Our results showed that metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 might be valuable markers in differentiating benign from malignant pleural effusions. Their levels are neither influenced by the histology and tumour origin nor by the presence of tumour cells in pleural effusions. Thus, their use in clinical practice could help in the selection of patients needing more invasive procedures, such as thoracoscopic biopsy. OBJECTIVES The aim of the present study was to evaluate the diagnostic accuracy of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in differentiating benign from malignant exudative pleural effusions. METHODS This is a unicentre observational study including 97 consecutive patients with exudative pleural effusions. Metalloproteinase-9, tissue inhibitor of metalloproteinase-1, lactate dehydrogenase, ferritin, carcinoembryonic antigen and carbohydrate antigen 15-3 were measured in pleural effusion and serum by enzyme-linked immunosorbent assay. The activity of metalloproteinase-9 was also evaluated by substrate zymography. The data were correlated with final diagnosis of pleural effusions to evaluate the diagnostic accuracy. RESULTS Of the 97 eligible patients, 6 were excluded. Of the 91 patients included in the study, 70 had malignant pleural effusions and 21 had benign pleural effusions. Both in sera and pleural effusions, matrix metalloproteinase-9 (P < 0.0001), tissue inhibitor of metalloproteinase-1 (P < 0.0001) and carcinoembryonic antigen (P < 0.0001) levels were higher in neoplastic patients than in benign group. Zymography analysis showed a most prominent band at a molecular weight of 92 kDa (metalloproteinase-9) whereas a less intense band was observed at 72 kDa (metalloproteinase-2). A significant correlation was found between metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 levels in pleural effusion (P < 0.0001; r = 0.8) and serum (P < 0.03; r = 0.2). Pleural effusion metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 levels showed higher value of sensitivity (97 and 91%, respectively) and specificity (90 and 95%, respectively) compared with other standard markers. Serum metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 levels showed similar results. Among 70 neoplastic patients, 29 had negative pleural cytology. Of these, 25 presented elevated levels of metalloproteinase-9 and tissue inhibitor of metalloproteinase-1, whereas 4 patients had elevated levels of one of the two markers. CONCLUSIONS Our results showed that metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 might be valuable markers in differentiating benign from malignant pleural effusions. Their levels are neither influenced by the histology and tumour origin nor by the presence of tumour cells in pleural effusions. Thus, their use in clinical practice could help in the selection of patients needing more invasive procedures, such as thoracoscopic biopsy.
- Published
- 2016
74. Substrate-zymography: a still worthwhile method for gelatinases analysis in biological samples
- Author
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Angelina Di Carlo, Serena Ricci, Francesco Oriente, Vittoria D'Esposito, Pietro Formisano, Ricci, Serena, D'Esposito, Vittoria, Oriente, Francesco, Formisano, Pietro, and DI CARLO, Angelina
- Subjects
Gelatinases ,food.ingredient ,Biochemistry (medical) ,Clinical Biochemistry ,Gelatinase A ,General Medicine ,Matrix (biology) ,Matrix metalloproteinase ,matrix metalloproteinase-2 ,Gelatin ,substrate zymography ,Extracellular matrix ,gelatinases ,matrix metalloproteinase-9 ,food ,Biochemistry ,Gelatinase ,Humans ,Zymography ,Polyacrylamide gel electrophoresis ,Human ,Enzyme Assays - Abstract
Matrix metallo-proteinases (MMPs) are a family of zinc-dependent endopeptidases, capable of degrading all the molecular components of extracellular matrix. A class of MMPs is gelatinases which includes gelatinase A or MMP-2 (72 kDa) and gelatinase B or MMP-9 (92 kDa), which have been shown to play critical roles in pathophysiology of many human disease and, in particular, cancer progression. For these reasons they obtained a great interest as potential non-invasive biomarker in providing useful clinical information in cancer diagnosis and therapy. A sensitive and unexpensive method for analysis of gelatinases is the gelatine zymography, which allows to measure the relative amounts of active and inactive enzymes in body fluids and tissue extracts. The procedure involves the electrophoretic separation of proteins under denaturing but non reducing conditions through a polyacrylamide gel containing a synthetic substrate (gelatin). The aim of this mini-review has been to describe the general principles of gelatine zymography technique, underling the main advantages and disadvantages. Even though an improvement of this method is necessary for a better applicability in laboratory medicine, gelatine zymography represents the most convenient method to detect the activity of the different gelatinases from a wide range of biological samples.
- Published
- 2015
75. Evaluation of MMP-2, MMP-9, TIMP-1, TIMP-2, NGAL and MMP-9/NGAL complex in urine and sera from patients with bladder cancer
- Author
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Angelina Di Carlo, Serena Ricci, Dario Bruzzese, Ricci, Serena, Bruzzese, Dario, and DI Carlo, Angelina
- Subjects
bladder carcinoma ,medicine.medical_specialty ,Pathology ,Urinary system ,Urine ,neutrophil gelatinase‑associated lipocalin ,Lipocalin ,matrix metalloproteinase-2 ,tissue inhibitor of metalloproteinase-2 ,Gastroenterology ,sera ,tissue inhibitor of metalloproteinase-1 ,Internal medicine ,matrix metalloproteinase-9 ,Carcinoma ,Medicine ,Bladder cancer ,Oncogene ,business.industry ,Cancer ,neutrophil gelatinase-associated lipocalin ,Articles ,Tissue inhibitor of metalloproteinase ,medicine.disease ,tissue inhibitor of metalloproteinase‑2 ,urine ,tissue inhibitor of metalloproteinase‑1 ,oncology ,cancer research ,business - Abstract
The identification of biomarkers in urine or serum samples from patients with bladder cancer is urgently required for the development of non-invasive methods for the diagnosis of bladder carcinoma and to facilitate follow-up surveillance, to combat the high progression and recurrence rates of this type of cancer. The current study measured the content of matrix metalloproteinase (MMP)-2 and -9, as well as tissue inhibitor of metalloproteinase (TIMP)-1 and -2 in the urine and sera of 41 patients with bladder cancer by ELISA. The association between levels of MMP-2 and -9 and TIMP-1 and -2, and tumor grade and stage were investigated to verify whether these molecules are involved in tumor differentiation. Statistical analysis of the data revealed that urinary TIMP-1 levels were significantly higher in the high grade group compared with those of the low grade samples (P=0.022). The results also revealed a significantly differing distribution of TIMP-1 expression between Ta and T1 stage specimens (P=0.040). The corresponding area under the curves (AUCs) were 0.72, with a sensitivity of 0.70 and specificity of 0.75. In addition, neutrophil gelatinase-associated lipocalin (NGAL) and MMP-9/NGAL complex levels in the sera were measured. All molecules evaluated were detected in the sera of the patients studied. In particular, tumors staged as non-muscle invasive (Ta and T1), demonstrated significantly higher NGAL levels compared with those of muscle invasive (>T1) bladder cancer (32.8 ng/ml vs. 16.2 ng/ml; P=0.029). The discriminatory ability of NGAL expression was confirmed by receiver operating characteristic curve analysis that revealed an AUC of 0.75, a sensitivity of 0.88 and a specificity of 0.67. These data indicated that urinary TIMP-1 and serum NGAL may be useful non-invasive biomarkers to provide clinical information for bladder cancer disease management. Multicenter, prospective studies are required to confirm these preliminary results.
- Published
- 2014
76. Design of a system to detect the force applied by tourniquets in a manikin's limb.
- Author
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Viola L, Lagazzi E, Ballardini G, Drogo A, Bonetti M, Marrone E, Chirico M, and Ricci S
- Subjects
- Hemorrhage therapy, Humans, Lower Extremity, Manikins, Tourniquets
- Abstract
Massive hemorrhage remains the number one cause of preventable death in trauma worldwide. However, immediate intervention by a bystander can significantly improve the survival of the injured person. In this context, the tourniquets represent the most quick and effective devices for stopping arterial and venous blood flow. The aim of this study was to implement a system to detect the force applied by a tourniquet on a simulated limb, without blood flow. The system we designed is characterized by four low-cost force sensing resistors placed on each lower limb of a manikin, below the groin. Tests on 21 tourniquets, revealed that our system is able to detect the force applied for 60 minutes, also discriminating between turns. Hence, this system can be used to compare the performance of different types of devices, but also to assess proper tourniquet placement in trainees and trauma care providers, thus making it a versatile low-cost device.
- Published
- 2021
- Full Text
- View/download PDF
77. RiNeo MR: A mixed-reality tool for newborn life support training.
- Author
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Ricci S, Mobilio GA, Calandrino A, Pescio M, Issa E, Rossi P, Chessa M, Solari F, Chirico M, and Casadio M
- Subjects
- Adult, Computer Simulation, Humans, Infant, Newborn, Manikins, Resuscitation, Augmented Reality, Virtual Reality
- Abstract
The first minute of life, the Golden Minute, has been defined as a critical window in which fundamental physiological processes occur for establishing spontaneous ventilation in a newborn. Resuscitation is more likely to succeed if it is performed properly and at the right time. In this scenario, simulation is an appropriate tool for training and evaluating the abilities of all staff working in the delivery room, as well as students. As simulations require a high degree of immersivity in order to be effective, the use of technologies like Virtual (VR) and mixed reality (MR) have garnered more interest in training. Currently, some VR and MR applications have been developed for adult life support training, but neonatal tools are still missing. To overcome this limitation, we present RiNeo MR, a prototype of a MR simulator for neonatal resuscitation training. The simulator consists of (i) a sensorized physical model of the newborn that allows monitoring chest compressions; (ii) a VR head mounted display that allows visualizing a virtual 3D model of the manikin and scenarios of the delivery and operating rooms. This enables students, and healthcare providers to be immersed in realistic hospital settings while performing life support procedures on the newborn manikin. Clinical Relevance-The newborn life support training (NLS) in facilities reduces term intrapartum-related deaths by 30%.
- Published
- 2021
- Full Text
- View/download PDF
78. Design and implementation of a low-cost birth simulator.
- Author
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Ricci S, Marcutti S, Pani A, Cordone M, Torre G, Vercelli GV, and Casadio M
- Subjects
- Female, Fetus, Humans, Parturition, Pregnancy, Cesarean Section, Delivery, Obstetric education, Obstetrics education, Simulation Training, Vacuum Extraction, Obstetrical
- Abstract
During spontaneous and operative deliveries it is important to correctly estimate the position and orientation of the fetus in the birth canal. In fact, incorrect evaluations can lead to errors in ventouse extraction, forceps application, and increased use of cesarean section. In this scenario, simulation is an appropriate tool for training and evaluating the abilities of gynecologists and midwives, because it allows student to practice both common situations and unlikely or risky events.Here we present eBSim, a prototype of a low-cost birth simulator that allows for precise identification of the fetal position, orientation, and station. The simulator consists on a sensorized physical model of the fetus and the pelvis, a corresponding virtual model, and an application, which allows students, instructors, and doctors to use the simulator for training and assessment of gynecological skills.
- Published
- 2019
- Full Text
- View/download PDF
79. Beta band frequency differences between motor and frontal cortices in reaching movements.
- Author
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Ricci S, Tatti E, Mehraram R, Panday P, and Ghilardi MF
- Subjects
- Adult, Electroencephalography, Electromyography, Female, Humans, Male, Middle Aged, Movement physiology, Psychomotor Performance physiology, Young Adult, Motor Cortex physiopathology
- Abstract
Movement is associated with power changes over sensory-motor areas in different frequency ranges, including beta (15-30 Hz). In fact, beta power starts decreasing before the movement onset (event-related desynchronization, ERD) and rebounds after its end (event-related synchronization, ERS). There is increasing evidence that beta modulation depth (measured as ERD-ERS difference) increases with practice in a planar reaching task, suggesting that this measure may reflect plasticity processes. In the present work, we analyzed beta ERD, ERS and modulation depth in healthy subjects to determine their changes over three regions of interest (ROIs): right and left sensorimotor and frontal areas, during a reaching task with the right arm. We found that ERD, ERS and modulation depth increased with practice with lower values over the right sensory-motor area. Timing of peak ERD and ERS were similar across ROIs, with ERS peak occurring earlier in later sets. Finally, we found that beta ERS of the frontal ROI involved higher beta range (23-29 Hz) than the sensory-motor ROIs (15-18 Hz). Altogether these results suggest that practice in a reaching task is associated with modification of beta power and timing. Additionally, beta ERS may have different functional meaning in the three ROIs, as suggested by the involvement of upper and lower beta bands in the frontal and sensorimotor ROIs, respectively.
- Published
- 2019
- Full Text
- View/download PDF
80. Effects of rTMS and intensive rehabilitation in Parkinson's Disease on learning and retention.
- Author
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Marchesi G, Albanese GA, Ferrazzoli D, George S, Ricci S, Tatti E, Di Rocco A, Quartarone A, Frazzitta G, and Ghilardi MF
- Subjects
- Aged, Female, Humans, Male, Middle Aged, Learning physiology, Parkinson Disease rehabilitation, Transcranial Direct Current Stimulation methods
- Abstract
Movement is accompanied by modulation of oscillatory activity in different ranges over the sensorimotor areas. This increase is more evident in normal subjects and less in patients with Parkinson's Disease (PD), a disorder associated with deficits in the formation of new motor skills. Here, we investigated whether such EEG changes improved in a group of PD patients, after two different treatments and whether this relates to performance. Subjects underwent either a session of 5 Hz repetitive Transcranial Magnetic Stimulation (rTMS) over the right posterior parietal cortex or a 4-week Multidisciplinary Intensive Rehabilitation Treatment (MIRT). We used a reaching task with visuo-motor adaptation to a rotated display in incremental 10° steps up to 60°. Retention of the learned rotation was tested before and after either intervention over two consecutive days. High-density EEG was recorded throughout the testing. We found that patients adapted their movements to the rotated display similarly to controls, although retention was poorer. Both rTMS and MIRT lead to improvement in retention of the learned rotation. Mean beta modulation levels changed significantly after MIRT and not after rTMS. These results suggest that rTMS produced local improvement reflected in enhanced short-term skill retention; on the other hand, MIRT determined changes across the contralateral sensorimotor area, reflected in beta EEG changes.
- Published
- 2019
- Full Text
- View/download PDF
81. Multifaceted Breast Cancer: The Molecular Connection With Obesity.
- Author
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Feola A, Ricci S, Kouidhi S, Rizzo A, Penon A, Formisano P, Giordano A, Di Carlo A, and Di Domenico M
- Subjects
- Animals, Breast Neoplasms complications, Breast Neoplasms genetics, Diabetes Mellitus, Type 2 complications, Humans, Obesity complications, Adipose Tissue metabolism, Breast Neoplasms metabolism, Diabetes Mellitus, Type 2 metabolism, Energy Metabolism physiology, Insulin Resistance physiology, Obesity metabolism
- Abstract
Obesity is characterized by a disruption in energy balance regulation that results in an excess accumulation of body fat. Its increasing prevalence poses a major public health concern because it is a risk factor for a host of additional chronic conditions, including type 2 diabetes, hypertension, and cardiovascular disease. Obesity is increasingly recognized as a growing cause of cancer risk. In particular excessive adipose expansion during obesity causes adipose dysfunction and inflammation that can regulate tumor growth. In obesity, dysregulated systemic metabolism and inflammation induce hyperinsulinemia, hyperglycemia, dyslipidemia, and enhance sex hormone production with increased secretion of proinflammatory adipokine that impact breast cancer development and progression. This review describes how adipose inflammation that characterizes obesity is responsible of microenvironment to promote cancer, and discuss how steroid hormones, that are essential for the maintenance of the normal development, growth and differentiation of the cells, influence the induction and progression of breast cancer. J. Cell. Physiol. 232: 69-77, 2017. © 2016 Wiley Periodicals, Inc., (© 2016 Wiley Periodicals, Inc.)
- Published
- 2017
- Full Text
- View/download PDF
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