63 results on '"Richou, Carine"'
Search Results
52. Genotyping Echinococcusmultilocularis in Human Alveolar Echinococcosis Patients: An EmsB Microsatellite Analysis.
- Author
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Knapp, Jenny, Gottstein, Bruno, Bretagne, Stéphane, Bart, Jean-Mathieu, Umhang, Gérald, Richou, Carine, Bresson-Hadni, Solange, and Millon, Laurence
- Subjects
ECHINOCOCCOSIS ,MICROSATELLITE repeats ,INCUBATION period (Communicable diseases) ,GENETIC markers ,CLINICAL epidemiology ,ECHINOCOCCUS multilocularis - Abstract
For clinical epidemiology specialists, connecting the genetic diversity of Echinococcusmultilocularis to sources of infection or particular sites has become somewhat of a holy grail. It is very difficult to trace the infection history of alveolar echinococcosis (AE) patients as there may be an incubation period of five to 15 years before reliable diagnosis. Moreover, the variability of parasitic manifestations in human patients raises the possibility of genetically different isolates of E. multilocularis having different levels of pathogenicity. Thus, the exposure of human patients to different strains or genotypes circulating in geographically different environments may lead to different disease outcomes. Molecular tools, such as the microsatellite marker EmsB, were required to investigate these aspects. This genetic marker was previously tested on a collection of 1211 European field samples predominantly of animal origin, referenced on a publicly available database. In this study, we investigated a panel of 66 metacestode samples (between 1981 and 2019) recovered surgically from 63 patients diagnosed with alveolar echinococcosis originating from four European countries (France, Switzerland, Germany, Belgium). In this study, we identified nine EmsB profiles, five of which were found in patients located in the same areas of France and Switzerland. One profile was detected on both sides of the French-Swiss border, whereas most patients from non-endemic regions clustered together in another profile. EmsB profiles appeared to remain stable over time because similar profiles were detected in patients who underwent surgery recently and patients who underwent surgery some time ago. This study sheds light on possible pathways of contamination in humans, including proximity contamination in some cases, and the dominant contamination profiles in Europe, particularly for extrahepatic lesions. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
- Full Text
- View/download PDF
53. Genotyping Echinococcus multilocularis in Human Alveolar Echinococcosis Patients: An EmsB Microsatellite Analysis
- Author
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Knapp, Jenny, Gottstein, Bruno, Bretagne, St��phane, Bart, Jean-Mathieu, Umhang, G��rald, Richou, Carine, Bresson-Hadni, Solange, and Millon, Laurence
- Subjects
570 Life sciences ,biology ,610 Medicine & health ,3. Good health - Abstract
For clinical epidemiology specialists, connecting the genetic diversity of Echinococcus multilocularis to sources of infection or particular sites has become somewhat of a holy grail. It is very difficult to trace the infection history of alveolar echinococcosis (AE) patients as there may be an incubation period of five to 15 years before reliable diagnosis. Moreover, the variability of parasitic manifestations in human patients raises the possibility of genetically different isolates of E. multilocularis having different levels of pathogenicity. Thus, the exposure of human patients to different strains or genotypes circulating in geographically different environments may lead to different disease outcomes. Molecular tools, such as the microsatellite marker EmsB, were required to investigate these aspects. This genetic marker was previously tested on a collection of 1211 European field samples predominantly of animal origin, referenced on a publicly available database. In this study, we investigated a panel of 66 metacestode samples (between 1981 and 2019) recovered surgically from 63 patients diagnosed with alveolar echinococcosis originating from four European countries (France, Switzerland, Germany, Belgium). In this study, we identified nine EmsB profiles, five of which were found in patients located in the same areas of France and Switzerland. One profile was detected on both sides of the French-Swiss border, whereas most patients from non-endemic regions clustered together in another profile. EmsB profiles appeared to remain stable over time because similar profiles were detected in patients who underwent surgery recently and patients who underwent surgery some time ago. This study sheds light on possible pathways of contamination in humans, including proximity contamination in some cases, and the dominant contamination profiles in Europe, particularly for extrahepatic lesions. more...
54. Screening of antigenic vesicular fluid proteins of Echinococcus multilocularis as potential viability biomarkers to monitor drug response in alveolar echinococcosis patients
- Author
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Valot, Benoît, Rognon, Bénédicte, Prenel, Anais, Baraquin, Alice, Knapp, Jenny, Anelli, Mathilde, Richou, Carine, Bresson-Hadni, Solange, Grenouillet, Frederic, Wang, Junhua, Vuitton, Dominique Angèle, Gottstein, Bruno, and Millon, Laurence more...
- Subjects
630 Agriculture ,570 Life sciences ,biology ,610 Medicine & health ,3. Good health - Abstract
PURPOSE The only drugs available to treat alveolar echinococcosis (AE) are mostly parasitostatic and in many cases prescribed for life. Decision criteria for discontinuation rely on the absence of parasitic viability. The aim of the present study is to search for candidate proteins that may exhibit good potential as biomarkers for viability. EXPERIMENTAL DESIGN Sixteen serum samples (five healthy controls, 11 patients with AE), are used. AE-patients are classified into three groups "Cured" (n = 2), "ABZ-responders" (n = 4) and "ABZ-nonresponders" (n = 5). Immunoreactive proteins from vesicular fluid (VF) are identified and quantified by LC-MS/MS analysis after immunoprecipitation (IP) using all 16 serum samples. RESULTS Shotgun analysis of VF lead to the identification of 107 E. multilocularis proteins. Comparative proteomics reveal nine proteins more abundant in IP eluates from ABZ-nonresponder patients (cathepsin b, prosaposin a preprotein, actin modulator protein, fucosidase alpha L1 tissue, gluthatione-S-tranferase, beta galactosidase, elongation factor 2, H17g protein tegumental antigen, and NiemannPick C2 protein). CONCLUSIONS AND CLINICAL RELEVANCE Detection of antibodies against these proteins by ELISA could be helpful to monitor the course of alveolar echinococcosis under albendazole (ABZ) treatment. more...
55. Is ex vivo liver resection and autotransplantation a valid alternative treatment for end-stage hepatic alveolar echinococcosis in Europe?
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Beldi, Guido, Vuitton, Dominique, Lachenmayer, Anja, Heyd, Bruno, Dufour, Jean-François, Richou, Carine, Candinas, Daniel, and Bresson-Hadni, Solange
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610 Medicine & health ,3. Good health
56. Investigating new serological and tissue markers for the follow-up of patients operated for alveolar echinococcosis
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Bellanger, Anne-Pauline, Wang, Junhua, Gbaguidi-Haore, Houssein, Barrera, Coralie, Bresson-Hadni, Solange, Zlobec, Inti, Lachenmayer, Anja, Richou, Carine, Turco, Celia, Gottstein, Bruno, Millon, Laurence, and Beldi, Guido more...
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570 Life sciences ,biology ,610 Medicine & health ,3. Good health - Abstract
AIMS Alveolar echinococcosis (AE) is characterized by a chronically progressing hepatic injury caused by Echinococcus multilocularis. Surgery presently remains the best curative option. Currently, biological predictive features derived from the resected specimens are not suitable to assess surgery efficacy. The present study was designed to investigate whether a selection of markers measured on the resected specimens exhibits predictive features related to parasite viability, or to a total elimination of the parasite, in addition to serological markers. METHODS AND RESULTS In a collaboration between two centers, one in France (Besançon), and one in Switzerland (Bern), samples from 40 AE patients were analyzed by microarray and serology techniques, individually. Paired serum samples before and after surgery were obtained for 26 patients. In the sera, a significant decrease of PD-L1 levels was observed after surgery, in addition to anti-Em18 levels. In the liver tissue, low levels of Cluster of Differentiation (CD)-3 were correlated with the absence of serum anti-Em18 after surgery. CONCLUSION This study showed PD-L1 is promising as a potential serological marker, and further confirmed the performance of anti-Em18 serology. Further studies on a larger cohort is needed to confirm the utility of performing systematically microarray on resected liver tissue. more...
57. Association between the dietary inflammatory index and the risk of hepatocellular carcinoma in a cirrhotic population.
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Rizk M, Roux-Levy C, Bernard-Chabert B, Bronowicki JP, Richou C, Habersetzer F, Jouve JL, Hebert JR, Shivappa N, Boutron-Ruault MC, Diab Assaf M, Hillon P, and Cottet V
- Abstract
Background & Aims: Hepatocellular carcinoma (HCC) is recognized as an inflammation-related cancer. However, the relation between inflammation deriving from the diet and HCC risk among cirrhotic patients has not yet been investigated. This study aimed to examine the association between the dietary inflammatory index (DII®) and HCC risk among cirrhotic patients., Methods: Clinical and dietary data were collected from the French case-control study CiRCE (Cirrhosis and Risk of hepatocellular Carcinoma in the East), which included 401 cirrhotic patients without HCC (controls) and 181 cirrhotic patients with HCC (cases) recruited between 2008 and 2012 in six French university hospitals. DII scores (36 food items and nutrients) were assessed using a validated self-administered diet history questionnaire. Odds ratios (OR) and 95 % confidence intervals (CI) were estimated with logistic regression models (adjusted for age, gender, time from cirrhosis diagnosis, etiology of cirrhosis, Child-Pugh score, and diabetes)., Results: The DII scores ranged between -4.77 and +7.59 with a mean value of 1.03 ± 2.87 among cases and 0.83 ± 2.87 among controls, indicating that both groups were following a pro-inflammatory diet. After multi-variable adjustment, there was a positive association between HCC risk and the DII score when considered as a continuous variable (OR
continuous = 1.14 [1.02-1.27], p-value = 0.021) or categorized in tertiles (ORT3vsT1 = 2.33 [1.16-4.67], p-trend = 0.021). Similar results were found when alcohol was omitted from the DII calculation but considered as a co-variate: ORcontinuous = 1.15 [1.03-1.28], p-value = 0.013, and ORT3vsT1 = 2.35 [1.18-4.68], p-trend = 0.015. A positive correlation was observed between the DII scores and two inflammatory biomarkers (CRP, IL-6) among controls., Conclusion: The present study reported an association between a pro-inflammatory DII score and the risk of HCC in cirrhotic patients. Correlations between the scores and biological parameters support a potential role for inflammation in HCC among cirrhotic. Result should be confirmed in larger prospective studies, and could lead to nutritional prevention in cirrhotic patients., Clinical Trial Registry: This study was registered in www., Clinicaltrials: gov as NCT01798173., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.) more...- Published
- 2024
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58. Fatty acid composition of the erythrocyte membrane and risk of hepatocellular carcinoma in cirrhotic patients.
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Mouillot T, Rizk M, Pais de Barros JP, Gilloteau A, Busson A, Bernard-Chabert B, Thiefin G, Barraud H, Bronowicki JP, Richou C, Di Martino V, Doffoel M, Minello A, Latournerie M, Jouve JL, Brondel L, Brindisi MC, Petit JM, Hillon P, and Cottet V more...
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- Aged, Biomarkers blood, Carcinoma, Hepatocellular epidemiology, Case-Control Studies, Female, Humans, Liver Cirrhosis epidemiology, Liver Neoplasms epidemiology, Male, Middle Aged, Odds Ratio, Phospholipids blood, Risk Factors, Carcinoma, Hepatocellular blood, Erythrocyte Membrane chemistry, Fatty Acids blood, Liver Cirrhosis blood, Liver Neoplasms blood
- Abstract
Background: Disturbances in fatty acid (FA) metabolism have been reported in cirrhosis, but the role of FAs in the development of hepatocellular carcinoma (HCC) is still unclear. Biomarkers are a promising means to explore the associations between exogenous intake or endogenous production of FAs and cancer risk., Aim: To estimate the relationship between fatty acid content in erythrocyte membranes and HCC risk in cirrhotic patients METHODS: The "CiRCE" case-control study recruited cirrhotic patients from six French hospitals between 2008 and 2012. Cases were cirrhotic patients with HCC (n = 349); controls were cirrhotic patients without HCC at inclusion (n = 550). FA composition of phospholipids in erythrocyte membranes was determined by high performance gas chromatography. Odds ratios for HCC risk according to FA concentrations were estimated with multivariable logistic regression., Results: HCC patients were older and more often men (P < 0.001). In both groups, saturated FAs represented more than 39% of all FAs in erythrocyte membranes, mono-unsaturated FAs around 14%, and polyunsaturated FAs around 46%. High levels of C15:0 + C17:0, C20:1 n-9, C18:2 n-6 and C20:2 n-6 were associated with higher risk of HCC. The levels of C18:0 and C20:4 n-6 were lower in HCC cases than in controls., Conclusions: The FA composition of erythrocyte membranes differed according to the presence of HCC with higher levels of saturated FAs, linoleic and eicosadienoic acids, and lower levels of stearic and arachidonic acids. These alterations may reflect particular dietary patterns and/or altered FA metabolism. Further investigations are warranted., (© 2020 John Wiley & Sons Ltd.) more...
- Published
- 2020
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59. Impact of Cytomegalovirus Infection on the Outcome of Patients With Cirrhosis: A Preliminary Study.
- Author
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Faivre M, Cottet V, Bour JB, Richou C, Valmary-Degano S, Thiefin G, Andreoletti L, Geist C, Schvoerer E, Malvé B, Habersetzer F, Fafi-Kremer S, Binquet C, Jouve JL, Bronowicki JP, Doffoel M, Hillon P, Herbein G, Monnet E, and Di Martino V more...
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- Carcinoma, Hepatocellular etiology, Carcinoma, Hepatocellular mortality, Case-Control Studies, Female, France epidemiology, Humans, Liver Neoplasms etiology, Liver Neoplasms mortality, Male, Middle Aged, Risk Factors, Carcinoma, Hepatocellular epidemiology, Cytomegalovirus Infections, Liver Cirrhosis, Liver Neoplasms epidemiology
- Abstract
Goals: The aims of this study were to evaluate whether cytomegalovirus (CMV) infection is associated with hepatocellular carcinoma (HCC) and liver-related mortality in cirrhotic patients., Background: In cirrhotic patients, the determinants of HCC and liver-related death are imperfectly known. CMV infection, by its prooncogenic and proinflammatory properties, may favor both the development of HCC and deleterious systemic inflammation., Study: In the 1178 patients included between June 2008 and December 2012 in the CIrrhose et Risque de Carcinome Hépatocellulaire dans le grand-Est (CIRCE) study, a French multicenter case-control study designed to identify risk factors of HCC among cirrhotic patients, we identified 432 patients with interpretable CMV serological status at baseline. They included 159 cases with HCC and 273 controls. We measured factors associated with HCC at baseline and subsequent HCC in controls, and predictors of overall and liver-related death in the whole study population., Results: During a median follow-up of 31 months, 25 cases of HCC developed in controls, and 209 deaths (163 liver-related) were recorded. There were 247 (57.2%) CMV-seropositive patients. CMV seropositivity was not associated with more frequent HCC at baseline or during follow-up, but among CMV-positive patients with HCC, the proportion of multinodular, infiltrative, or metastatic tumors at diagnosis was higher (73.8% vs. 57.3%; P=0.029), inducing higher mortality (74% vs. 52% at 3 years; P=0.004). By Cox-regression adjusted for age, gender, Model for End-stage Liver Disease (MELD) score, HCC at baseline, and diabetes, CMV seropositivity independently predicted all-cause (hazard ratio=1.45; 95% confidence interval, 1.08-1.94; P=0.013) and liver-related mortality (hazard ratio=1.56; 95% confidence interval, 1.04-2.30; P=0.031)., Conclusions: In this preliminary study, CMV-seropositive cirrhotic patients were at higher risk of liver-related death caused by more aggressive HCCs or severe cirrhosis complications. These findings warrant confirmation. more...
- Published
- 2019
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60. Screening of antigenic vesicular fluid proteins of Echinococcus multilocularis as potential viability biomarkers to monitor drug response in alveolar echinococcosis patients.
- Author
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Valot B, Rognon B, Prenel A, Baraquin A, Knapp J, Anelli M, Richou C, Bresson-Hadni S, Grenouillet F, Wang J, Vuitton DA, Gottstein B, and Millon L
- Subjects
- Adult, Aged, Aged, 80 and over, Albendazole therapeutic use, Animals, Blotting, Western, Echinococcus multilocularis drug effects, Female, Humans, Male, Middle Aged, Biomarkers metabolism, Echinococcosis drug therapy, Echinococcus multilocularis metabolism
- Abstract
Purpose: The only drugs available to treat alveolar echinococcosis (AE) are mostly parasitostatic and in many cases prescribed for life. Decision criteria for discontinuation rely on the absence of parasitic viability. The aim of the present study is to search for candidate proteins that may exhibit good potential as biomarkers for viability., Experimental Design: Sixteen serum samples (five healthy controls, 11 patients with AE), are used. AE-patients are classified into three groups "Cured" (n = 2), "ABZ-responders" (n = 4) and "ABZ-nonresponders" (n = 5). Immunoreactive proteins from vesicular fluid (VF) are identified and quantified by LC-MS/MS analysis after immunoprecipitation (IP) using all 16 serum samples., Results: Shotgun analysis of VF lead to the identification of 107 E. multilocularis proteins. Comparative proteomics reveal nine proteins more abundant in IP eluates from ABZ-nonresponder patients (cathepsin b, prosaposin a preprotein, actin modulator protein, fucosidase alpha L1 tissue, gluthatione-S-tranferase, beta galactosidase, elongation factor 2, H17g protein tegumental antigen, and NiemannPick C2 protein)., Conclusions and Clinical Relevance: Detection of antibodies against these proteins by ELISA could be helpful to monitor the course of alveolar echinococcosis under albendazole (ABZ) treatment., (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) more...
- Published
- 2017
- Full Text
- View/download PDF
61. BRIP1 coding variants are associated with a high risk of hepatocellular carcinoma occurrence in patients with HCV- or HBV-related liver disease.
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Oussalah A, Avogbe PH, Guyot E, Chery C, Guéant-Rodriguez RM, Ganne-Carrié N, Cobat A, Moradpour D, Nalpas B, Negro F, Poynard T, Pol S, Bochud PY, Abel L, Jeulin H, Schvoerer E, Chabi N, Amouzou E, Sanni A, Barraud H, Rouyer P, Josse T, Goffinet L, Jouve JL, Minello A, Bonithon-Kopp C, Thiefin G, Di Martino V, Doffoël M, Richou C, Raab JJ, Hillon P, Bronowicki JP, and Guéant JL more...
- Abstract
The molecular mechanisms of hepatocellular carcinoma (HCC) carcinogenesis are still not fully understood. DNA repair defects may influence HCC risk. The aim of the study was to look for potential genetic variants of DNA repair genes associated with HCC risk among patients with alcohol- or viral-induced liver disease. We performed four case-control studies on 2,006 European- (Derivation#1 and #2 studies) and African-ancestry (Validation#1 and #2 studies) patients originating from several cohorts in order to assess the association between genetic variants on DNA repair genes and HCC risk using a custom array encompassing 94 genes. In the Derivation#1 study, the BRIP1 locus reached array-wide significance (Chi-squared SV-Perm, P =5.00×10
-4 ) among the 253 haplotype blocks tested for their association with HCC risk, in patients with viral cirrhosis but not among those with alcoholic cirrhosis. The BRIP1 haplotype block included three exonic variants (rs4986763, rs4986764, rs4986765). The BRIP1 ' AAA ' haplotype was significantly associated with an increased HCC risk [odds ratio (OR), 2.01 (1.19-3.39); false discovery rate (FDR)- P =1.31×10-2 ]. In the Derivation#2 study, results were confirmed for the BRIP1 ' GGG ' haplotype [OR, 0.53 (0.36-0.79); FDR- P =3.90×10-3 ]. In both Validation#1 and #2 studies, BRIP1 ' AAA ' haplotype was significantly associated with an increased risk of HCC [OR, 1.71 (1.09-2.68); FDR- P =7.30×10-2 ; and OR, 6.45 (4.17-9.99); FDR- P =2.33×10-19 , respectively]. Association between the BRIP1 locus and HCC risk suggests that impaired DNA mismatch repair might play a role in liver carcinogenesis, among patients with HCV- or HBV-related liver disease., Competing Interests: CONFLICTS OF INTEREST The authors who have taken part in this study declared that they do not have anything to disclose regarding funding or conflict of interest with respect to this manuscript. more...- Published
- 2016
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62. Detection, isolation, and characterization of alpha-fetoprotein-specific T cell populations and clones using MHC class I multimer magnetic sorting.
- Author
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Pichard V, Royer PJ, Richou C, Cauchin E, Goebes K, Gaignerie A, Masliah C, Gournay J, Gregoire M, and Ferry N
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- Aged, Aged, 80 and over, Carcinoma, Hepatocellular immunology, Carcinoma, Hepatocellular pathology, Cytotoxicity Tests, Immunologic, Female, HLA-A Antigens isolation & purification, HLA-A Antigens metabolism, HLA-A2 Antigen, Humans, Leukocytes, Mononuclear immunology, Leukocytes, Mononuclear metabolism, Leukocytes, Mononuclear pathology, Liver Neoplasms immunology, Liver Neoplasms pathology, Male, Middle Aged, Peptides chemistry, Peptides immunology, T-Cell Antigen Receptor Specificity, T-Lymphocyte Subsets pathology, alpha-Fetoproteins immunology, HLA-A Antigens immunology, Immunomagnetic Separation, T-Lymphocyte Subsets immunology, alpha-Fetoproteins analysis, alpha-Fetoproteins metabolism
- Abstract
alpha-fetoprotein (AFP) is a fetal protein specifically reexpressed in 50% of hepatocellular carcinomas. This protein could serve as a tumor-associated antigen for immunotherapy purpose. The aim of our work was to analyze the presence of AFP-specific T cell populations in peripheral blood mononuclear cells (PBMCs) from cirrhotic patients with or without hepatocellular carcinoma. Using peptide-major histocompatibility complex class I multimers, AFP-specific populations corresponding to 3 previously described human leukocyte antigen (HLA)-A*0201 major histocompatibility complex class I epitopes (AFP137, AFP158, and AFP325) were sorted magnetically from CD8 positive cells without prior stimulation with the target antigen. T cell populations specific for 1 peptide (AFP158) were frequent, whereas populations corresponding to peptide AFP137 were rare and absent for peptide AFP325. We also isolated and fully characterized T cell clones specific for AFP137 and AFP158 peptides. We show that these clones can be used to monitor dendritic cell loading with peptides and could be useful for future immunotherapy protocols. more...
- Published
- 2008
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63. [Treatment of chronic hepatitis C: side effects, tolerability and quality of life].
- Author
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Gournay J and Richou C
- Subjects
- Anemia, Hemolytic chemically induced, Antiviral Agents administration & dosage, Blood Cell Count, Clinical Trials as Topic, Controlled Clinical Trials as Topic, Depressive Disorder chemically induced, Drug Therapy, Combination, Follow-Up Studies, Hepatitis C, Chronic blood, Humans, Interferon-alpha administration & dosage, Kidney Transplantation, Liver Transplantation, Mental Disorders chemically induced, Neutropenia chemically induced, Prospective Studies, Quality of Life, Retrospective Studies, Ribavirin administration & dosage, Risk Factors, Thrombocytopenia chemically induced, Time Factors, Antiviral Agents adverse effects, Hepatitis C, Chronic drug therapy, Interferon-alpha adverse effects, Ribavirin adverse effects
- Published
- 2002
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