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The Cerro Prieto basin, a tectonically active pull-apart basin, hosts significant geothermal resources currently being exploited in the Cerro Prieto Geothermal Field (CPGF). Consequently, natural tectonic processes and anthropogenic activities contribute to three-dimensional surface displacements in this pull-apart basin. Here, we obtained the Cerro Prieto Step-Over 3D surface velocity field (3DSVF) by accomplishing a weighted least square algorithm inversion from geometrically quasi-orthogonal airborne UAVSAR and RADARSAT-2, Sentinel 1A satellite Synthetic Aperture-Radar (SAR) imagery collected from 2012 to 2016. The 3DSVF results show a vertical rate of 150 mm/yr and 40 mm/yr for the horizontal rate, where for the first time, the north component displacement is achieved by using only the Interferometric SAR time series in the CPGF. Data integration and validation between the 3DSVF and ground-based measurements such as continuous GPS time series and precise leveling data were achieved. Correlating the findings with recent geothermal energy production revealed a subsidence rate slowdown that aligns with the CPGF's annual vapor production. [ABSTRACT FROM AUTHOR]

The objective of this study was to examine the interactions between anionic surfactants, specifically 1-alkylsulfonates (KXS) and 1-alkylsulfates (SXS) ions, with human serum albumin (HSA). A combination of experimental techniques, including isothermal titration calorimetry (ITC), steady-state fluorescence spectroscopy (SF), and molecular dynamics-based approaches was employed to gain a comprehensive understanding of these processes. It has been demonstrated that the subtle variations in the charge distribution on the anionic surfactant headgroups have a significant impact on the number of binding sites, the stoichiometry of the resulting complexes, and the strength of the interactions between the surfactants and the protein. Additionally, we established that the affinity of the investigated ligands to specific regions on the protein surface is governed by both the charge of the surfactant headgroup and the length of the aliphatic hydrocarbon chain. In summary, the findings highlight the crucial role of charge distribution on surfactant functional groups in the binding mode and the thermodynamic stability of surfactant–protein complexes. [ABSTRACT FROM AUTHOR]

On 16 April 2013, an M w = 7.7 earthquake struck the border of Iran and Pakistan in the central part of the Makran subduction zone with a reported depth of 80 km by USGS. This rare event in this poorly instrumented region helps to shed light on the kinematics of the subducting slab. We investigate source parameters of the Saravan intraslab normal earthquake using RADARSAT-2 SAR images in three ascending tracks, nine permanent GNSS sites and teleseismic data. The maximum coseismic displacement occurred at the SRVN GNSS station with 54.1 mm southeast horizontal and 42.7 mm upward vertical displacements. The coseismic ascending InSAR displacement maps illustrate a continuous and smooth NE-trending elliptical shape deformation pattern with a maximum of ∼29 cm of displacement away from the satellite. We use 25 broad-band teleseismic P-waveforms to estimate the focal mechanism of the main shock. A joint uniform inversion of InSAR, GNSS and teleseismic data reveals a NW-dipping SW-striking fault and a primarily normal-faulting earthquake with a minor right-lateral strike-slip component. The static slip distribution of the InSAR coseismic maps localizes variable slip at depths between 50 and 81 km with a maximum amplitude >3 m at 60–75.5 km depth, rupturing the oceanic crust of the subducted slab. The kinematic slip distribution exhibits a well-constrained slip pattern with a nucleation depth of 65 km. The source time function indicates that the earthquake reaches its maximum moment tensor release at ∼8 and ∼16 s. The NE-trend of the Saravan earthquake slip pattern, the orientation of the volcanic arc, and the distribution of the intraslab intermediate-depth normal earthquakes provide new insights into slab geometry in the central Makran subduction zone. We suggest that the slab bending at the hinge of subducting Arabian Plate is oblique along a NE–SW direction parallel to the volcanic arc rather than the shoreline or deformation front, and it is likely to be the reason for an oblique volcanic arc in the Makran subduction zone. These new constraints on the Makran slab geometry will help further studies in establishing realistic coupling maps for seismic hazard assessment. [ABSTRACT FROM AUTHOR]

Glycosaminoglycans (GAGs) play a key role in a variety of biological processes in the extracellular matrix (ECM) via interactions with their protein targets. Due to their high flexibility, periodicity and electrostatics-driven interactions, GAG-containing complexes are very challenging to characterize both experimentally and in silico. In this study, we, for the first time, systematically analyzed the interactions of endostatin, a proteolytic fragment of collagen XVIII known to be anti-angiogenic and anti-tumoral, with heparin (HP) and representative heparan sulfate (HS) oligosaccharides of various lengths, sequences and sulfation patterns. We first used conventional molecular docking and a docking approach based on a repulsive scaling–replica exchange molecular dynamics technique, as well as unbiased molecular dynamic simulations, to obtain dynamically stable GAG binding poses. Then, the corresponding free energies of binding were calculated and the amino acid residues that contribute the most to GAG binding were identified. We also investigated the potential influence of Zn2+ on endostatin–HP complexes using computational approaches. These data provide new atomistic details of the molecular mechanism of HP's binding to endostatin, which will contribute to a better understanding of its interplay with proteoglycans at the cell surface and in the extracellular matrix. [ABSTRACT FROM AUTHOR]

Acridinium esters, due to their capability for chemiluminescence (CL), are employed as indicators and labels in biomedical diagnostics and other fields. In this work, the influence of ionic surfactants, hexadecyltrimethylammonium chloride and bromide (CTAC and CTAB, cationic) and sodium dodecyl sulphate (SDS, anionic) on the CL parameters and mechanism of representative emitter, 10-methyl-9-[(2-methylphenoxy)carbonyl]acridinium trifluoromethanesulphonate (2MeX) in a H2O2/NaOH environment, is studied. Our investigations revealed that the type of surfactant and its form in solution have an impact on the CL kinetic constants and integral efficiencies, while changes in those emission properties resulting from the type of ion (Cl− vs. Br−) are negligible. The major changes were recorded for systems containing surfactants at concentrations higher than the critical micelle concentration. The cationic surfactants (CTAC, CTAB) cause a substantial increase in CL emission kinetics and a moderate increase in its integral efficiency. At the same time, the opposite effect is observed in the case of SDS. Molecular dynamics simulations suggest that changes in emission parameters are likely due to differences in the binding strength of 2MeX substrate with surfactant molecules, which is higher for SDS than for CTAC. The results can help in rational designing of optimal acridinium CL systems and demonstrate their usefulness in distinguishing the pre- and post-micellar environment and the charge of surfactants. [ABSTRACT FROM AUTHOR]

The space weather impact on the health status of people with cardiovascular diseases is quite significant and it has been shown by many researchers. To examine this relationship at high latitudes, we made a Yakutsk ambulance electronic database analysis. The significant coincidences of geomagnetic disturbance indicators (Kp-index - a global geophysical disturbance index) and the increased blood pressure emergency calls number have been determined during the high solar activity years. The blood pressure increase cases analysis shows that patients with primary essential arterial hypertension (AH) seek medical help in 57%. Furthermore, 7% of AH patients experienced a hypertensive crisis (HC) with an increase in systolic blood pressure (SBP) from 180 mm Hg, diastolic blood pressure (DBP) from 120 mm Hg. The medical data analysis and the space weather data (Kp-index) comparison demonstrate a significant coincidence, which became the main base for the conclusion that heliogeophysical disturbance effects on the cardiovascular system state of a person living at high latitudes.

NMR spectroscopy techniques can provide important information about protein‐ligand interactions. Here we tested an NMR approach which relies on the measurement of paramagnetic relaxation enhancements (PREs) arising from analogous cationic, anionic or neutral soluble nitroxide molecules, which distribute around the protein‐ligand complex depending on near‐surface electrostatic potentials. We applied this approach to two protein‐ligand systems, interleukin‐8 interacting with highly charged glycosaminoglycans and the SH2 domain of Grb2 interacting with less charged phospho‐tyrosine tripeptides. The electrostatic potential around interleukin‐8 and its changes upon binding of glycosaminoglycans could be derived from the PRE data and confirmed by theoretical predictions from Poisson‐Boltzmann calculations. The ligand influence on the PREs and NMR‐derived electrostatic potentials of Grb2 SH2 was localized to a narrow protein region which allowed the localization of the peptide binding pocket. Our analysis suggests that experiments with nitroxide cosolutes can be useful for investigating protein‐ligand electrostatic interactions and mapping ligand binding sites. [ABSTRACT FROM AUTHOR]