51. Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications.
- Author
-
Jiang, Su, Liu, Ya-Feng, Wang, Xiao-Min, Liu, Ke-Fei, Zhang, Ding-Hong, Li, Yi-Ding, Yu, Ai-Ping, Zhang, Xiao-Hui, Zhang, Jia-Yi, Xu, Jian-Guang, Gu, Yu-Dong, Xu, Wen-Dong, and Zeng, Shao-Qun
- Subjects
Engineering ,Physical Sciences ,Biomedical Engineering ,Neurosciences ,(220.0220) Optical design and fabrication ,(230.4685) Optical microelectromechanical devices ,(250.3140) Integrated optoelectronic circuits ,Optical Physics ,Materials Engineering ,Ophthalmology and optometry ,Biomedical engineering ,Atomic ,molecular and optical physics - Abstract
We introduce a more flexible optogenetics-based mapping system attached on a stereo microscope, which offers automatic light stimulation to individual regions of interest in the cortex that expresses light-activated channelrhodopsin-2 in vivo. Combining simultaneous recording of electromyography from specific forelimb muscles, we demonstrate that this system offers much better efficiency and precision in mapping distinct domains for controlling limb muscles in the mouse motor cortex. Furthermore, the compact and modular design of the system also yields a simple and flexible implementation to different commercial stereo microscopes, and thus could be widely used among laboratories.
- Published
- 2016