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357 results on '"Shen, Chenjia"'

63. The Complete Chloroplast Genomes of Two Physalis Species, Physalis macrophysa and P. ixocarpa : Comparative Genomics, Evolutionary Dynamics and Phylogenetic Relationships.

Author

Zhang, Zhenhao, Jin, Yanyun, Gao, Yadi, Zhang, Yong, Ying, Qicai, Shen, Chenjia, Lu, Jiangjie, Zhan, Xiaori, Wang, Huizhong, and Feng, Shangguo

Subjects
CHLOROPLAST DNA, COMPARATIVE genomics, PHYSALIS, ORNAMENTAL plants, SPECIES, TRANSFER RNA
Abstract

Physalis macrophysa (PMA) and Physalis ixocarpa (PIX) have significant economic, medicinal, and ornamental value and are often used in the fields of edible fruits, medicinal herbs, and ornamental plants. In the present study, we obtained the complete chloroplast (cp) genome sequences of PMA and PIX, compared to the cp genome sequences of 10 Physalis species, and constructed the phylogenetic tree among the tribe Physaleae. The results showed that the cp genomes of PMA and PIX consisted of a large single copy (LSC) region (87,115 bp and 87,103 bp, respectively), a small single copy (SSC) region (18,412 bp and 18,420 bp, respectively), and a pair of same-length inverted-repeat (IRa and IRb) regions (25,604 bp and 25,674 bp, respectively). The two species contained 132 genes, including 87 encoding proteins, eight encoding ribosomal RNAs (rRNAs), and 37 encoding transfer RNAs (tRNAs), which indicated that the two species have strong similarities with respect to genome structure and gene content. PMA and PIX contained repeat sequences (35 and 40, respectively) and simple-sequence repeats (SSRs) (61 and 60, respectively). Nine regions with considerable nucleotide divergence were found, most of which were located in the LSC and SSC regions. The gene selective pressure analysis indicated that eight genes were affected by positive selection, the Ka/Ks values of which were greater than one. Our phylogenetic results indicated that PMA and PIX had the closest genetic relationship and are closely adjacent to Physalis philadelphica (PPH) in the subtribe Physalinae. Our analysis of the cp genomes in both Physalis species will be beneficial for further research into species identification, phylogeny, evolution, and the potential for germplasm resource exploitation in Physalis. [ABSTRACT FROM AUTHOR]

Published
2023
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71. Tissue‐specific study across the stem of Taxus media identifies a phloem‐specific TmMYB3 involved in the transcriptional regulation of paclitaxel biosynthesis

Author

Yu, Chunna, primary, Luo, Xiujun, additional, Zhang, Chengchao, additional, Xu, Xinyun, additional, Huang, Jiefang, additional, Chen, Yueyue, additional, Feng, Shangguo, additional, Zhan, Xiaori, additional, Zhang, Lei, additional, Yuan, Huwei, additional, Zheng, Bingsong, additional, Wang, Huizhong, additional, and Shen, Chenjia, additional

Published
2020
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74. Comparative metabolomic and transcriptomic analyses revealed the differential accumulation of secondary metabolites during the ripening process of acerola cherry (Malpighia emarginata) fruit.

Author

Xu, Mingfeng, Shen, Chenjia, Zhu, Qin, Xu, Yunsheng, Xue, Changfeng, Zhu, Beiwei, and Hu, Jiangning

Subjects
*METABOLITES, *FRUIT ripening, *METABOLOMICS, *FRUIT, *CHALCONE synthase, *TRANSCRIPTOMES, *CHERRIES, *SWEET cherry
Abstract

BACKGROUND Acerola cherry is a famous functional fruit containing plentiful antioxidants and other nutrients. However, studies on the variations among nutrients during the ripening process of acerola fruit are scare. RESULTS: Comparative metabolomic and transcriptomic analyses were performed and identified 31 331 unigenes and 1896 annotated metabolite features in acerola cherry fruit. K Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that several antioxidant and nutrient‐related metabolic pathways, such as the flavonoids, vitamins, carotenoids, amino acids, and fatty acids metabolic pathways, were significantly changed during the ripening process. The metabolites related to the vitamin, carotenoid, and fatty acid metabolic pathways were downregulated during the ripening process. Several flavonoid biosynthesis‐related genes (including dihydroflavonol 4‐reductase, chalcone synthase, flavanone 3‐hydroxylase, and anthocyanidin synthase), were significantly upregulated, suggesting their essential functions in the accumulation of flavonoids in mature fruit. CONCLUSION: Most of the vitamin and carotenoid metabolism‐related metabolites significantly accumulated in immature fruit, suggesting that immature acerola fruit is a good material for the extraction of vitamins and carotenoids. For macronutrients, most of the amino acids accumulated in mature fruit and most of the fatty acids greatly accumulated in immature fruit. Our data revealed the differential accumulation of antioxidants and nutrients during the ripening process of acerola cherry fruit. © 2021 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Published
2022
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78. Bioinformatics, bacterial expression and enzyme activity analyses of dichloromethane dehalogenase from Methylobacterium rhodesianum H13.

Author

Ni, Jianguo, Liu, Ying, Shen, Chenjia, Chen, Dongzhi, Xin, Yueyong, and Liu, Qi

Subjects
BACTERIAL enzymes, IMMOBILIZED proteins, DICHLOROMETHANE, METHYLOBACTERIUM, AMINO acid sequence
Abstract

Dechlorination is the key step in biological degradation of dichloromethane (DCM), which is catalyzed by the enzyme DCM dehalogenase. In this study, the detailed structure and function of DCM dehalogenase in Methylobacterium rhodesianum H13 were investigated. Our results indicate that DCM dehalogenase (C1505H2255N403O430S7) is a stable hydrophilic protein localized in the cytoplasm, with an isoelectric point of 6.35. They also indicate that DCM dehalogenase is a frizzled protein with 52.43% of amino acid residues exposed on its surface, and that alpha-helices are a main structural component (53.47%). As one of the theta class glutathione S-transferases (GSTs), DCM dehalogenase presents as a dimeric structure and shows strong substrate selectivity. The catalytic tunnel radius and length of DCM dehalogenase are both 1.4 Å. Glu42 and Val43 are two of the essential amino acids in both the catalytic tunnel and catalytic pockets. The amino acid sequence of DCM dehalogenase was predicted to be homologous among various microorganisms, and its active site was conserved with eight potential sites. The recombinant DCM dehalogenase was expressed in E. coli BL21 strain. The optimum conditions for DCM dehalogenase are pH 8, temperature of 30 °C and presence of Mn2+. All the obtained information of DCM dehalogenase will lay the foundation for further study of its structural transformation and functional improvement. [ABSTRACT FROM AUTHOR]

Published
2020
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83. High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae)

Author

Lu, Jiangjie, primary, Liu, Yuyang, additional, Xu, Jing, additional, Mei, Ziwei, additional, Shi, Yujun, additional, Liu, Pengli, additional, He, Jianbo, additional, Wang, Xiaotong, additional, Meng, Yijun, additional, Feng, Shangguo, additional, Shen, Chenjia, additional, and Wang, Huizhong, additional

Published
2018
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92. The auxin response factor, OsARF19, controls rice leaf angles through positively regulating OsGH3-5 and OsBRI1

Author

Zhang, Saina, Wang, Suikang, Xu, Yanxia, Yu, Chenliang, Shen, Chenjia, Qian, Qian, Geisler, Markus, Jiang, De An, and Qi, Yanhua

Subjects
fungi, food and beverages
Abstract

Auxin and brassinosteroid (BR) are important phytohormones for controlling lamina inclination implicated in plant architecture and grain yield. But the molecular mechanism of auxin and BR crosstalk for regulating lamina inclination remains unknown. Auxin response factors (ARFs) control various aspects of plant growth and development. We here report that OsARF19-overexpression rice lines show an enlarged lamina inclination due to increase of its adaxial cell division. OsARF19 is expressed in various organs including lamina joint and strongly induced by auxin and BR. Chromatin immunoprecipitation (ChIP) and yeast one-hybrid assays demonstrate that OsARF19 binds to the promoter of OsGH3-5 and brassinosteroid insensitive 1 (OsBRI1) directing their expression. OsGH3-5-overexpression lines show a similar phenotype as OsARF19-O1. Free auxin contents in the lamina joint of OsGH3-5-O1 or OsARF19-O1 are reduced. OsGH3-5 is localized at the endoplasmic retieulum (ER) matching reduction of the free auxin contents in OsGH3-5-O1. osarf19-TDNA and osgh3-5-Tos17 mutants without erected leaves show a function redundancy with other members of their gene family. OsARF19-overexpression lines are sensitive to exogenous BR treatment and alter the expressions of genes related to BR signalling. These findings provide novel insights into auxin and BR signalling, and might have significant implications for improving plant architecture of monocot crops.

Published
2014

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