51. Analysis of <italic>Vibrio</italic> seventh pandemic island II and novel genomic islands in relation to attachment sequences among a wide variety of <italic>Vibrio cholerae</italic> strains.
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Nguyen, Tuan Hai, Pham, Tho Duc, Higa, Naomi, Iwashita, Hanako, Takemura, Taichiro, Ohnishi, Makoto, Morita, Kouichi, and Yamashiro, Tetsu
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VIBRIO cholerae ,MICROBIAL virulence ,NUCLEOTIDE sequencing ,PANDEMICS ,INTEGRASES - Abstract
ABSTRACT:
Vibrio cholerae O1 El Tor, the pathogen responsible for the current cholera pandemic, became pathogenic by acquiring virulent factors includingVibrio seventh pandemic islands (VSP)‐I and −II. Diversity of VSP‐II is well recognized; however, studies addressing attachment sequence left (attL ) sequences of VSP‐II are few. In this report, a wide variety ofV. cholerae strains were analyzed for the structure and distribution of VSP‐II in relation to their attachment sequences. Of 188V. cholerae strains analyzed, 81% (153/188) strains carried VSP‐II; of these, typical VSP‐II, and a short variant was found in 36% (55/153), and 63% (96/153), respectively. A novel VSP‐II was found in twoV. cholerae non‐O1/non‐O139 strains. In addition to the typical 14‐bpattL , six newattL ‐like sequences were identified. The 14‐bpattL was associated with VSP‐II in 91% (139/153), whereas the remaining six types were found in 9.2% (14/153) ofV. cholerae strains. Of note, six distinct types of theattL ‐like sequence were found in the seventh pandemic wave 1 strains; however, only one or two types were found in the wave 2 or 3 strains. Interestingly, 86% (24/28) ofV. cholerae seventh pandemic strains harboring a 13‐bpattL ‐like sequence were devoid of VSP‐II. Six novel genomic islands using two unique insertion sites to those of VSP‐II were identified in 11V. cholerae strains in this study. Four of those shared similar gene clusters with VSP‐II, except integrase gene. [ABSTRACT FROM AUTHOR]- Published
- 2018
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