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Background/aims: Lacerations of the oral mucosa and fractures of alveolar processes commonly occur in traumatic dental injuries (TDIs). Impaired wound healing and tissue regeneration have severe consequences on the quality of life. Bone marrow mesenchymal stem cells (BMMSCs) possess the ability of self-renewal and multipotential differentiation. Treatment with low-level sodium fluoride (NaF) has emerged as a promising approach to enhance wound repair. The aim of this study was to assess the effects of low-level NaF on soft tissue healing and on the proliferation, migration and extracellular matrix synthesis of BMMSCs.Material and Methods: BMMSCs derived from mice were treated with 50 μM, 500 μM, or 5 mM NaF for 12, 24, and 48 hours, and cell proliferation was assessed by the MTS assay. Cell motility was detected at 12 and 24 hours by a wound healing assay, and osteoblastic differentiation for 21 days by 1% Alizarin Red S staining in 50 μM NaF-treated BMMSCs. Gene expression of Runx2 and Osteocalcin was evaluated by quantitative real-time PCR. An experimental rat skin wound model was employed, and levels of c-Myc, Ki67, fibronectin, and vimentin were assessed by immunohistochemistry.Results: There was a significant induction in the proliferation and migration of BMMSCs treated with 50 μM NaF. The expression of Ki67 and c-Myc protein was increased in tissues treated with 50 μM NaF, and the expression of fibronectin and vimentin in the 50 μM NaF-treated tissues was stimulated. Alizarin Red staining revealed enhanced mineralization in 50 μM NaF-treated BMMSCs with increased expression of Runx2 and Osteocalcin, indicating their upregulated osteogenic differentiation.Conclusion: Low-level NaF could promote soft tissue healing and hard tissue regeneration. [ABSTRACT FROM AUTHOR]

• Finite element analysis has multitude of applications in biomechanics and biophysics to study the mechanical behaviour of tissues or organs. • Inconsistencies in reporting such studies have been thereby affecting their reliability and reproducibility. • We have developed standard reporting-guidelines for biomechanical studies to bring about a consistency in reporting such studies so that no significant information related to the FE model or FE analysis is missed. • These reporting guidelines can help in improving the details of these studies so as to facilitate ease in understanding and extrapolation of the results in clinical scenario. To the best of our knowledge, there are no reporting guidelines for design, conduct and reporting of Finite Element studies in health sciences. We intend to propose specific and detailed guidelines for reporting these studies. After recognizing the need to have uniform guidelines for reporting of finite element analysis in medicine and dentistry, a group of 5 researchers working on FEA as their research area met in the summer of 2020 and drafted the methodology for the development of such guidelines. Each researcher individually made a list of major headings required for reporting these studies and met again in September 2020 to finalize the domains. Subsequently, sub headings and details were charted. The draft list of items for reporting the guidelines were presented to a larger team of 15 experts and some changes were further made based on their inputs. The guidelines entail seven major domains and their sub-domains, including parameters for model structure, segmentation, mesh structure, force application and model validation, etc. This checklist aims to improvise the reporting and consistency of FEA studies. We hope that the usage and adoption of these guidelines by the scientific community would result in more thoughtful and uniform documentation. Also, the confidence in the results would be enhanced through model reproducibility, reusability and accountability. The proposed guidelines were named as 'Reporting of in-silico studies using finite element analysis in medicine' and the term 'RIFEM' was used as acronym. [ABSTRACT FROM AUTHOR]