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In this paper, the collapse of a void bubble filled with vapor content is numerically investigated using a novel moving particle semi-implicit with meshless advection by flow-directional local grid (MPS-MAFL) method. The interfacial velocity, collapse time, bubble shape variation, peak pressure, rebound bubble radius, and other interesting parameters were obtained and are discussed profoundly. The vapor bubble undergoes several cycles of oscillation with reduced amplitude during the whole collapse process, which is similar to cavitation bubble collapse. The computational results show that the bubble collapse time is linearly proportional to the initial bubble size, which agrees with the Rayleigh equation. The minimum rebound bubble radius ratio is less affected by initial bubble size for a large bubble. Comparison work was also conducted against experimental data by Board and Kimpton. The comparison revealed that the MPS method supplied with an adiabatic compression assumption for vapor content is more suitable to evaluate the collapse behaviors of a low-pressure vapor bubble. This work is helpful for further application of the moving particle semi-implicit with meshless advection using flow-directional local grid (MPS-MAFL) method to solving complicated bubble dynamics. [ABSTRACT FROM AUTHOR]

Tibial dyschondroplasia (TD) is characterized by expansion of the proximal growth plates of the tibiotarsus that fail to form bone, lack blood vessels, and contain non-viable cells. Thiram (a carbamate pesticide), when fed to young broiler chicks, induces TD with high regularity and precision. We used this experimental model to understand the cause of the defects associated with TD by selecting and identifying the genes differentially expressed in the TD growth plate of broiler chickens. Broiler chicks at 7 days of age were randomly divided into two groups. After fasting overnight, they were fed with regular diet (control) or the same diet containing 100 mg/kg thiram for 96 h to induce TD (thiram-fed). mRNA was purified from the growth plates of control and thiram-fed broilers. Forward and reverse-subtracted cDNA libraries were generated by suppression subtractive hybridization technology. Ten selected genes from cDNA libraries were identified by real-time quantitative polymerase chain reaction. All were differentially expressed in TD growth plates (P<0.05 or P<0.01). The levels of collagen type X (Col X), pro-alpha-1 collagen type I (Col I α1), collagen type IX (Col IX), NADH dehydrogenase (NADH DH), cytochrome C oxidase subunit III (COX III), enolase 1, alpha (ENO1), carbonic anhydrase II (CA2) and heat shock protein 90 (Hsp90) mRNA transcripts were up-regulated, while the expression levels of Matrilin 3 (MATN3) and chondromodulin-I (ChM-I) were down-regulated. Col I and Hsp90 were detected by immunohistochemistry at different stages. Given that these genes are involved in matrix formation, endochondral ossification, developmental regulation, electron transport in the mitochondrial respiratory chain and vascularization, our findings may provide new insights into understanding the pathogenesis of TD. [ABSTRACT FROM AUTHOR]