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This study presents a sensor strip for user-friendly, naked-eye detection of Xylella fasitdiosa, the bacterial causal agent of Pierce’s disease in grapevine. This sensor uses anti–X. fastidiosaantibodies conjugated to a polydiacetylene layer on a polyvinylidene fluoride strip to generate specific color transitions and discriminate levels of the pathogen. The detection limit of the sensor is 0.8 × 108cells/mL, which is similar to bacterial load in grapevine 18 days following bacterial inoculation. This sensor enables equipment-free detection that is highly desirable for in-field diagnostic tools in resource-limited settings.

Low-cost and quick detection of biotic stresses is critically important for protection of staple food crops such as maize in smallholder farms in developing countries, where access to improved seed varieties, fertilizers, and pesticides is limited due to financial and geographical reasons. Here, we report a new lateral flow detection technology directly integrated in a maize leaf, in which microspheres conjugated with analyte-specific capture antibodies are non-invasively injected. The antibody-conjugated microspheres capture and detect an analyte in a concentration-specific manner. In this study, we optimized microsphere size for effective infiltration and immobilization in the leaf, and further demonstrated detection of a fluorescent mock biomarker, fluorescein, in a live maize plant. This in plantalateral flow biosensor is the first of its kind and is expected to provide a low-cost and user-friendly detection method for biotic stresses in the field.

The article explores the author's experience regarding the use of the Hippotizer Stage media server with projectors in stage productions. He cites the student production "The Balcony," which was held in a black box theater at the University of Texas in El Paso, Texas. He enumerates the issues that the design team encountered which include on where the images could be projected, finding the right images for the production, and on how to go into projection.

The article describes the lighting and projection designs of the theater production "The Threepenny Opera" hosted by the theater and dance department of the University of Texas at El Paso (UTEP) in Texas. The staff at the UTEP used the Titan HD-600 Projector by Digital Projection Inc. and rear projection (RP) screen for the projection surface of the play. The staff also considered the color temperature of the projection lamp and the color that was projected on the screen.