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This study evaluates the impact of biochar addition on the performance of anaerobic co-digestion of food waste (FW) and sewage sludge at different total solids (TS) contents (2.5 %, 5.0 %, and 7.5 %). Biochar co-digestion improved hydrolysis and acidogenesis by neutralizing volatile fatty acids (VFAs) reducing its inhibitions (2.6-fold removal), which elevated the soluble chemical oxygen demand (sCOD) degradation by 2.5 folds leading to a higher cumulative methane production compared to the control. This increase corresponded to an improvement of methane yields by ∼21 %-33 % (242-340 mL/gVS add ) at different TS contents. The biochar surface area offered substantial support for direct interspecies electron transfer (DIET) activity, and biofilm-mediated growth of methanogens i.e., Methanosarcina, Methanosata, and Methanobrevibacter. The biochar-enriched digestate improved the seed germination index, and bioavailability of plant nutrients such as N, P, K, and NH 4 +- N. This study reports an improved biochar-mediated anaerobic co-digestion for efficient and sustainable FW valorization., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Pharmaceuticalsare a diverse group of chemical compounds widely used for prevention and treatment of infectious diseases in both humans and animals. Pharmaceuticals, either in their original or metabolite form, find way into the wastewater treatment plants (WWTPs) from different sources. Recently, anaerobic membrane bioreactors (AnMBR) has received significant research attention for the treatment of pharmaceuticals in various wastewater streams. This review critically examines the behaviour and removal of a wide array of pharmaceuticals in AnMBR with primary focus on their removal efficiencies and mechanisms, critical influencing factors, and the microbial community structures. Subsequently, the inhibitory effects of pharmaceuticals on the performance of AnMBR and membrane fouling are critically discussed. Furthermore, the imperative role of membrane biofouling layer and its components in pharmaceuticals removal is highlighted. Finally, recent advancements in AnMBR configurations for membrane fouling control and enhanced pharmaceuticals removal are systemically discussed., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

This work reported a new waste functionalization and utilization method, which use digestate to prepare hydrochar to improve methane production from food waste (FW) and sewage sludge (SS). Experimental results presented that 10 g/L hydrochar obtained the cumulative methane production of 133.11 ± 1.18 mL/g volatile solids added, 26.99 % higher than that without hydrochar addition. By monitoring the conversion of model metabolic intermediates, 10 g/L hydrochar was determined to favor hydrolysis, acidogenesis and methonogenesis bio-processes involved in methane production, thus improving the degradation of solubilized organics and consumption of short-chain fatty acids (SCFAs) during the co-digestion. Microbial investigation revealed that 10 g/L hydrochar enriched the microbes relevant to methane production (e.g., Methanosaeta and Syntrophomonas), but reduced the abundances of hydrolysis- and acidogenesis-related microbes (e.g., Acinetobacter). This hydrochar-based preparation and utilization strategy might offer a novel paradigm for waste-control-waste, bringing economic and environmental benefits., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Reaching industrially relevant productivities in bioprocesses and their efficient integration in the existing industrial infrastructure remain as important challenges in the circular economy to create closed loop sustainability framework. Using anaerobic digestion (AD) biorefinery as a model, the present work addressed these problems via integration of next-generation rhamnolipids production with AD. A high rhamnolipids concentration of 10.25 ± 1.34 g/L was obtained by fed-batch fermentation using food waste digestate as medium. Digestate-derived rhamnolipids contained Rha-C 10 -C 10 and Rha-Rha-C 10 -C 10 as the predominant congeners. These were used back in single-phase AD to demonstrate their effect on sludge solubilization and digestion efficiency. A dosage of 0.02 g rhamnolipids/g total suspended solids was found to be optimal which enhanced the hydrolysis-acidogenesis reactions to up to 27% over control. It however retarded methane production which could be overcome by the prolongation of digestion time. Finally, the value chain appreciation by the proposed process was demonstrated by a feasibility analysis., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Composting of food waste digestate (FWD) is challenging as it requires more bulking agents, and the nitrogen loss is inevitable. To address these issues, FWD composting was conducted at a relatively lower C/N ratio of 15 with zeolite amendment in the dosage range of 5-15%. The impact of zeolite addition on nitrogen loss, NH 3 and N 2 O emissions was assessed during FWD composting. The results showed that the addition of 10-15% zeolite could significantly reduce the phytotoxic nature of FWD and the compost maturity level could be reached in 10-21 days. Furthermore, ∼45% total nitrogen loss could be reduced by mitigating NH 3 and N 2 O emissions upon 10 and 15% zeolite amendment. The outcome of the present study could be used as an effective strategy for composting FWD in any part of the world as the FWD characteristics are similar irrespective of the type of food waste., (Copyright © 2022. Published by Elsevier Ltd.)

Mold contamination is one of the most important causes for indoor air pollution. Previous studies have indicated the feasibility of employing wet-dry cycles, a special water treatment approach in indoor environments, to control indoor mold contamination. However, the underlying mechanisms regulating the responses of indoor molds to changing moisture conditions remains to be elucidated. Here, we studied the mechanisms regulating the responses to wet-dry cycles (termed as moisture dynamics) in Aspergillus penicillioides, Cladosporium cladosporioides, and Aspergillus niger. First, the dormant spores of each mold species were grown to the swollen stage. Next, swollen spores were incubated at different water activity (a w ) levels (0.4, 0.6 and 0.8 a w ) for up to 15 days. Afterward, the viability, lipid peroxidation and antioxidant activities (both enzymatic and non-enzymatic) of treated molds were determined. Our results show that the mold species that survived better under moisture dynamics also encountered less oxidative damage and exhibited stronger antioxidant activities. Moreover, lower RH imposed severer oxidative stress to C. cladosporioides and A. niger. Pearson correlation coefficient indicate significant correlations between oxidative stress and a w of dry periods, oxidative damage and mold survival, as well as oxidative responses and mold survival. Collectively, these results imply that oxidative stress adaptation regulates the viability of A. penicillioides, C. cladosporioides, and A. niger in response to moisture dynamics. Our findings facilitate the development of novel engineering solutions for indoor air pollution., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Recently, conductive materials (i.e., carbon-based and iron-based materials) as a feasible and attractive approach have been introduced to anaerobic co-digestion (ACoD) system for promoting its performance and stability through direct interspecies electron transfer. Owing to the key roles of conductive materials in ACoD process, it is imperative to gain a profound understanding of their specific functions and mechanisms. Here, this review critically examined the state of the art of conductive materials assisted ACoD of food waste and common municipal organic solid waste. Then, the fundamental roles of conductive materials on ACoD enhancement and the relevant mechanisms were discussed. Last, the perspectives for co-digestate treatment, reutilization, and disposal were summarized. Moreover, the main challenges to conductive materials amended ACoD in on-site application were proposed and the future remarks were put forward. Collectively, this review poses a scientific basis for the potential application of conductive materials in ACoD process in the future., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Biotransformation of organic substrates via acidogenic fermentation (AF) to high-value products such as C1-C6 carboxylic acids and alcohol serves as platform chemicals for various industrial applications. However, the AF technology suffers from low product titers due to thermodynamic constraints. Recent studies suggest that augmenting AF redox potential can regulate the metabolic pathway and provide seamless electron flow by lowering the activation energy barrier, thus positively influencing the substrate utilization rate, product yield, and speciation. Hence, the augmented AF system with an exogenous electricity supply is termed as electro-fermentation (EF), which has enormous potential to strengthen the fermentation technology domain. Therefore, this critical review systematically discusses the current understanding of EF with a special focus on the extracellular electron transfer mechanism of electroactive bacteria and provides perspectives and research gaps to further improve the technology for green chemical synthesis, sustainable waste management, and circular bio-economy., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

This study systematically evaluated and compared different inoculum pretreatment methods to quickly select dark fermentative bacteria from anaerobic sludge for the bioconversion of food waste. The hydrogen (H 2 ) production rate was found to be highest for 'heat + CO 2 ' treated inoculum at 140.75 ± 2.61 mL/L/h compared to control experiments (60.27 ± 2.61 mL/L/h). At the same time, H 2 yield was found to be highest for alkali-treated inoculum at 157.25 ± 7.62 mL/g of volatile solids (VS) added compared to control experiments (91.61 ± 1.93 mL/g VS). Analysis of organic acids suggests a Clostridial-type fermentation with acetate (0.52 to 1.60 g/L) and butyrate (1.69 to 2.42 g/L) being the major by-products. The microbial data analysis showed that Firmicutes (63.64-90.39%), Bacteroidota (1.16-21.88%), and Proteobacteria (2.09-9.93%) were dominant at the phylum level, whereas genus-level classification showed Clostridium sensu stricto 1 (6.37-42.63%), Streptococcus (1.87-28.96%), Prevotella (0.57-16.59%), and Enterococcus (0.56-14.51%) dominated under different experimental conditions., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Red tourism, as a form of special interest tourism (SIT), becomes widespread among Chinese tourists. This study aims to explore memorable tourism experiences (MTEs) in red tourism destinations and examines how country competence affects intention to visit similar destinations through the influences on MTEs, destination image, red tourism place attachment, and overall satisfaction. The partial least squares structural equation modeling (PLS-SEM) analysis is utilized to analyze the data from 556 tourists. Empirical results reveal that country competence positively influences MTEs and destination image. Furthermore, both MTEs and destination image influence overall satisfaction and red tourism place attachment, but do not influence intention to visit other similar destinations; the relationships between overall satisfaction, intention to visit other similar destinations, and place attachment are all confirmed as well. This study represents one of the few studies that classify red tourism as a form of historical-based SIT, with the purpose of promoting country competence. The outcomes provide a better understanding of both scholars and practitioners of historical-based SIT planning and businesses on how to promote destination image and country competence., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Zhou, Wong and Wang.)

Temperature is known as one of the abiotic factors that can affect mold growth. Many mold growth prediction models consider temperature as one of the parameters that can significantly impact mold growth indoors, and hence temperature has been targeted by different indoor mold prevention strategies on different premises. For example, European guidelines for libraries suggest a temperature of 19 °C to preserve books. However, running low temperature air-conditioning (AC) costs substantially more energy, and thus a higher temperature (e.g., 25.5 °C) has been regularly proposed as the recommended indoor temperature for general indoor environments in Hong Kong. It is, therefore, needed to understand whether or not the reduction of indoor temperature would lead to better effectiveness of mold prevention. Using Cladosporium cladosporioides ( C. cladosporioides ) as the model, its germinating spores were challenged in C. cladosporioides to wet-dry cycles with different combinations of relative humidity (RH, 40%, 60% and 80%) and temperature (19 °C and 28 °C) levels. The survival, lipid peroxidation and catalase (CAT) activity of the treated spores were monitored and compared. C. cladosporioides spores showed similar levels of viability, lipid peroxidation and CAT activity when they were exposed to 19 °C and 28 °C at the same RH, but substantially lower survival and higher oxidative stress were observed under the wet-dry cycles with 40% RH dry periods compared with 60% and 80% RH at both temperatures, suggesting that indoor temperature does not tend to affect the resistance of C. cladosporioides to wet-dry cycles as significantly as the RH level of the dry period. Collectively, this study suggests a more important role for moisture over temperature in indoor mold prevention. The outcome of this study may facilitate the sustainable management of indoor mold problems in buildings.

Soil lead (Pb) contamination is often caused by anthropogenic activities. In this study, a pot experiment was conducted to assess the effect of biochars derived from pig-carcass (PCBC) and branches of oriental-plane tree (OPBC) on the bioavailability, redistribution, and phytoavailability of Pb and P, as well as the growth of Ipomoea aquatica Forsk in a Pb-contaminated soil. Application of PCBC increased the total and available P concentrations in the soil as compared to the control, and enhanced the concentrations of labile P and sparingly labile P via direct exogenous P input and improvement of soil pH. Both biochars facilitated P accumulation in plant shoots and roots. Sequential extraction of soil Pb confirmed that biochar application facilitated the transformation of mobile Pb into stable fractions, with greater effects from PCBC than OPBC. Hence, biochar application significantly decreased the soil DTPA-extractable Pb by 90.2% (PCBC) and 64.0% (OPBC) compared to the control, consequently reducing Pb uptake by plants. The Pb immobilization by biochar was driven by the biochar-induced increase of soil pH, Pb-phosphate/carbonate precipitation, ion exchange between Pb 2+ and biochar-derived cations (e.g., Ca 2+ and K + ), and surface complexation with functional groups (e.g., carboxyl, hydroxyl, CO). Application of PCBC simultaneously increased the biomass of plant roots and shoots, by 1.8- and 0.6- folds, respectively. Overall, PCBC showed a potential to function as an effective amendment in the immobilization of Pb and alternative P fertilizer to improve degraded soils., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Wastewater is one of the most common by-products of almost every industrial process. Treatment of wastewater alone, before disposal, necessitates an excess of energy. Environmental concerns over the use of fossil fuels as a source of energy have prompted a surge in demand for alternative energy sources and the development of sophisticated procedures to extract energy from unconventional sources. Treatment of municipal and industrial wastewater alone accounts for about 3% of global electricity use while the amount of energy embedded in the waste is at least 2-4 times greater than the energy required to treat the same effluent. The microbial electrolysis cell (MEC) is one of the most efficient technologies for waste-to-product conversion that uses electrochemically active bacteria to convert organic matter into hydrogen or a variety of by-products without polluting the environment. This paper highlights existing obstacles and future potential in the integration of Microbial Electrolysis Cell with other processes like anaerobic digestion coupled system, anaerobic membrane bioreactor and thermoelectric micro converter.

The population growth, along with lifestyle changes, has resulted in unprecedented levels of food waste at all phases of the supply chain, including harvest, packing, transportation, and consumption. Conventional practices involve dumping of food waste with municipal garbage. However, these methods have serious environmental and health consequences. Food waste has a great recycling perspective due to its high biodegradability and water content, making it an ideal substrate for the production of biofuels and other industrially important chemicals including pigments, enzymes, organic acids, and essential oils. This review extensively covers conversion of food waste to generate bioenergy which will help to reduce environmental pollution and facilitate implementation of a circular bioeconomy. Moreover, review also highlights novel technologies like supercritical fluid extraction, ultra-sonication, pressurized liquid extraction, and microwave assisted extractions that are being employed in food waste management to increase the efficiency of value-added product recovery in an economically viable manner. Metabolic engineering of microorganisms for specificity of product would be a future breakthrough in food waste valorization/management., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Majority of industries, in order to meet the technological development and consumer demands generate waste. The untreated waste spreads out toxic and harmful substances in the environment which serves as a breeding ground for pathogenic microorganisms thus causing severe health hazards. The three industrial sectors namely food, agriculture, and oil industry are among the primary organic waste producers that affect urban health and economic growth. Conventional treatment generates a significant amount of greenhouse gases which further contributes to global warming. Thus, the use of microbes for utilization of this waste, liberating CO 2 offers an indispensable tool. The simultaneous production of value-added products such as bioplastics, biofuels, and biosurfactants increases the economics of the process and contributes to environmental sustainability. This review comprehensively summarized the composition of organic waste generated from the food, agriculture, and oil industry. The linkages between global health hazards of industrial waste and environmental implications have been uncovered. Stare-of-the-art information on their subsequent utilization as a substrate to produce value-added products through bio-routes has been elaborated. The research gaps, economical perspective(s), and future research directions have been identified and discussed to strengthen environmental sustainability., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

In present investigation, effect of diverting acidogenic off-gas from leached bed reactor (LBR) to up-flow anaerobic sludge blanket (UASB) reactor during semi-continuous food waste (FW) anaerobic digestion was evaluated. In test LBR headspace pressure (3.3 psi) was maintained with intermittent headspace gas transfer into UASB. In control, same headspace pressure was maintained without gas transfer. The semi-continuous FW addition affected the characteristics and production of leachate in control and test LBR. The cumulative COD, total soluble products and methane yields were 1.26, 1.37 and 3 times higher in the test LBR than the control. The acetate and methane yields from test LBR were 697.8 g·kgVS added -1 and 167.55 mL·gCOD -1 feeding. Acidogenic gas transfer maintained low partial pressure of hydrogen and the hydrogen to carbon-di-oxide ratio in the headspace of LBR, which were thermodynamically favorable for microbial metabolism and concomitant high-rate production of acetate-rich volatile fatty acid and methane-rich biogas from FW., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Effects of biochar on co-digestion of pig manure and dewatered sewage sludge under different total solids (TS) were investigated. Biochar could accelerate the start-up of methanogenesis and shorten the adaptation phase. At TS5%, the methane daily production in biochar group was 60.6% higher than the control; nevertheless, when TS increased, the gap between two groups gradually narrowed. Additionally, the change on antibiotics resistance genes (ARGs) was also affected by TS and the biochar addition. Moreover, biochar was beneficial to reduce ARGs in liquid phase. At TS14%, the total ARGs abundance in the liquid phase of biochar group was 41.4% lower than the control, among which the reduction rates of etB(P), sul1, rpoB2, macA, mupA and mupB were more prominent. These findings could provide useful guidance for developing ARGs elimination strategy before their release into the environment., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

The high-nitrogen content and dense structure of poultry manure compost cause volatilization of N to ammonia (NH 3 ). This study evaluated the combined application of biochar and biotrickling filtration (BTF) to remove of odor in chicken manure mixed straw compost (w/w, 2.5:1). Adding of 10% biochar reduced NH 3 , hydrogen sulfide (H 2 S), and total volatile organic compounds (TVOCs) contents by 20.04%, 16.18%, and 17.55% respectively, and decreased the N loss rate by 8.27%, compared with those observed in control. The organic matter content decreased by 28.11% and germination index reached 97.36% in the experimental group. Meanwhile, the N-cycling microorganisms such as Pusillimonas and Pseudomonas became more active, and the relative abundance of sulfur-cycling microorganisms Hydrogenispora decreased in the experimental group. Following BTF application, the NH 3 , H 2 S, and TVOCs removal rates reached 95%, 97%, and 53%, respectively., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Rapid urbanization has increased the demand for food, feed, and chemicals that have in turn augmented the use of fossil-based resources and generation of organic waste. Owning to the characteristics like high abundance, renewability, and ease of accessibility, valorization of organic wastes serves as a potential solution for waste management issues. Several industrial wastes, due to their organic and nutrient-rich composition, have been utilized as a resource for the production of value-added products such as biofuels, biopesticides, biohydrogen, enzymes, and bioplastics via microbial fermentation processes. The process consists of pre-treatment of the waste biomass, production of value-added product in reactors and downstream processing for product's recovery. The integration of new comprehensive technologies for organic waste utilization will also stimulate the transition towards a circular economy. Therefore, the feasibility and sustainability of the production of various value-added products from biowastes and byproduct streams will be discussed in the present review., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Waste generation is becoming a global concern owing to its adverse effects on environment and human health. The utilization of waste as a feedstock for production of value-added products has opened new avenues contributing to environmental sustainability. Microorganisms have been employed for production of biosurfactants as secondary metabolites by utilizing waste streams. Utilization of waste as a substrate significantly reduces the cost of overall process. Biosurfactant(s) derived from these processes can be utilized in environmental and different industrial sectors. This review focuses on global market of biosurfactants followed by discussion on production of biosurfactants from waste streams such as agro-industrial waste and waste cooking oil. The need for waste stream derived circular bioeconomy and scale up of biosurfactant production have been narrated with applications of biosurfactants in environment and industrial sectors. Road blocks and future directions for research have also been discussed., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

In the last two decades, water use has increased at twice the rate of population growth. The freshwater resources are getting polluted by contaminants like heavy metals, pesticides, hydrocarbons, organic waste, pathogens, fertilizers, and emerging pollutants. Globally more than 80% of the wastewater is released into the environment without proper treatment. Rapid industrialization has a dramatic effect on developing countries leading to significant losses to economic and health well-being in terms of toxicological impacts on humans and the environment through air, water, and soil pollution. This article provides an overview of physical, chemical, and biological processes to remove wastewater contaminants. A physical and/or chemical technique alone appears ineffective for recovering useful resources from wastewater containing complex components. There is a requirement for more processes or processes combined with membrane and biological processes to enhance operational efficiency and quality. More processes or those that are combined with biological and membrane-based processes are required to enhance operational efficiencies and quality. This paper intends to provide an exhaustive review of electrochemical technologies including microbial electrochemical technologies. It provides comprehensive information for the recovery of metals, nutrients, sulfur, hydrogen, and heat from industrial effluents. This article aims to give detailed information into the advancements in electrochemical processes to energy use, improve restoration performance, and achieve commercialization. It also covers bottlenecks and perspectives of this research area.

High concentration of NH 4 + -N in food waste digestate (FWD) produced from biological treatment of food waste is considered as a major threat on the composting process resulting in production of immature compost. Hence, a laboratory batch composting study was conducted to examine the feasibility of using biochar as a physical additive to ameliorate the inhibitory effect of NH 4 + -N and to mitigate the nitrogen loss during FWD composting. FWD was co-composted with tobacco biochar at a dosage of 0%, 2.5%, 5% or 10% (dw) in bench-scale composters with a controlled aeration system. The addition of 10% biochar enhanced the degradation rate resulting in 44% higher carbon decomposition than the control. Besides, 10% biochar amendment reduced NH 3 and N loss by 58% and 5%, respectively and significantly reduced NH 4 + -N content to HKORC limit of < 700 mg/kg dw within 5 days showing the beneficiary impact of biochar addition., (Copyright © 2021. Published by Elsevier Ltd.)

Direct land application of food waste digestate (FWD) leads to 60-70% of nitrogen loss through NH 3 volatilization due to its innate characteristics like high ammonium nitrogen (NH 4 + -N) (~6000 mg/kg dry matter) and high moisture content (~75%). Hence, bio stabilization of FWD through composting is a promising solution to curb the environmental and occupational hazards. Hence the aim of this study was to assess the feasibility of using sawdust and/or mature compost as a bulking agent to achieve effective composting. The results showed that mixing of FWD with sawdust alone or together with mature compost could produce quality compost with reduced NH 4 + -N (<700 mg/kg dry matter) and increased seed germination index (>80%) within 2 weeks of co-composting. Composting FWD with both sawdust and mature compost effectively reduced ~ 83% of NH 3 volatilization demonstrating that this approach can effectively produce mature nitrogen enriched FWD compost., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

In this research, we investigated and compared the effects of three widely used conductive materials, e.g., zero-valent iron (Fe 0 ), magnetite (Fe 3 O 4 ), and biochar on the performance, stability, and in-depth mechanism during the anaerobic co-digestion process of sewage sludge and food waste. Among the three conductive materials, Fe 0 could achieve the highest cumulative methane production of 394.0 mL/g volatile solids (VS) added , which was 1.24-fold and 1.17-fold higher than that receiving Fe 3 O 4 and biochar. The mechanistic studies indicated that compared to the Fe 3 O 4 and biochar groups, Fe 0 could significantly enhance the release of soluble protein, polysaccharide, and dissolved organic matters, the degradation of volatile fatty acids and VS, and the activities of key enzymes and direct interspecies electron transfer (DIET). Consequently, the methane yield and digestate dewaterability were notably improved. Collectively, these findings will offer suggestions of the preferable conductive materials in the anaerobic co-digestion process for decision makers., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Literature shows that biochar can potentially retain nutrients in agricultural soils, avoiding significant nutrient losses. Furthermore, biochar porosity and functional groups have been shown to enhance physico-chemical properties of soil when amended, which in turn has the ability to encourage inhabitation of specific microorganisms as biofertilizers or to enhance soil remediation. It supports scale-dependent parameters and provides both ecosystem services and soil-vegetation solutions relevant to nature-based solutions. However, detailed researches on the mechanisms of soil microbial interactions with biochar porous properties are required, along with the microbial attachment factors, sustenance, and detachment when applied to soils. Recent valuable works have impregnated plant growth-promoting bacteria unto biochar and have observed inconsistent results. Firstly, biochar intrinsic properties alter the fate of impregnation by inhibiting quorum sensing signals, and the macropore requirements for adsorption and/or biofilm formation have not been well considered. Additionally, the nutrient and supplement requirements for each microorganism as well as the adsorption capacity have not been well understood for biochar surfaces. Substantial information is required to understand the mechanisms of microbe adsorption and factors that influence the process, as well as sustenance of the matrix even when deployed in soils. Research directions should focus on determining molecular and chemical mechanisms responsible for the biochar-microbe interaction process and fate of microbe on biochar while expressing plant growth-promoting properties, which needs to be done in laboratory and field trials. Graphical abstract., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

An integrated two-phase AD with acidogenic off-gas diversion from a leach bed reactor to an upflow anaerobic sludge blanket was developed for improving methane production. However, this system had its own technical limitation such as mass transfer efficiency for solid-state treatment. In order to optimize the mass transfer in this two phase AD system, leachate recirculation with various water replacement rates regulating the total solids contents (TS) at 12.5%, 15%, and 17.5% was aim to investigate its effect on methane generation. The solubilization of food waste was increased with decreasing TS content, while the enzymatic hydrolysis showed the opposite trend. A TS contents of 15% presented the best acidogenic performance with the highest hydrogen yield of 30.3 L H 2 /kg VS added , which subsequently resulted in the highest methane production. The present study provides an easy approach to enhance food waste degradation in acidogenic phase and energy conversion in methanogenic phase simultaneously., (Copyright © 2021. Published by Elsevier Ltd.)

Food waste digestate is a by-product of the anaerobic digestion of food waste. Presence of high ammonium nitrogen content significantly increase the nitrogen loss upon direct application on soil or by conventional composting. In this review, a comprehensive discussion regarding the effective management of food waste digestate is outlined, in which global food waste digestate production, characteristics, and composting are discussed. The nitrogen dynamics cycle considering high ammonium nitrogen content in the digestate is also evaluated, including ammonification, nitrification, denitrification, and other possible mechanisms based on the current literature. Mitigation strategies for reducing nitrogen loss via C/N ratio adjustment and the addition of physical, chemical, and microbial amendments were evaluated and estimated for 15 countries based on the available data on food waste anaerobic digestion plants. Reduced nitrogen loss and high quality compost could be produced from food waste digestate by adapting mitigation strategies., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

This work investigated the impact of the addition of different biochar types on mitigation of volatile fatty acid (VFA) accumulation, methane recovery and digestate quality in mesophilic food waste-sludge co-digestion. Four biochars derived from agricultural and sludge residues under different pyrolysis temperatures were compared. Specific biochar properties such as pH, surface area, chemical properties and presence of surface functional groups likely influenced biochar reactions during digestion, thereby resulting in a varying performance of different biochars. Miscanthus straw biochar addition led to the highest specific methane yield of 307 ± 0.3 mL CH 4 /g VS added versus 241.87 ± 5.9 mL CH 4 /g VS added from control with no biochar addition over 30 days of the co-digestion period. Biochar supplementation led to enhanced process stability which likely resulted from improved syntrophic VFA oxidation facilitated by specific biochar properties. Overall, a 21.4% increase in the overall methane production was obtained with biochar addition as compared to control. The resulting digestate quality was also investigated. Biochar-amended digester generated a digestate rich in macro- and micro-nutrients including K, Mg, Ca, Fe making biochar-amended digestate a potential replacement of agricultural lime fertilizer. This work demonstrated that the addition of specific biochars with desirable properties alleviated VFA accumulation and facilitated enhanced methane recovery, thereby providing a means to achieve process stability even under high organic loading conditions in co-digestions. Moreover, the availability of biochar-enriched digestate with superior characteristics than biochar-free digestate adds further merit to this process., (Copyright © 2021. Published by Elsevier Ltd.)

The quest for a chemical surfactant substitute has been fuelled by increased environmental awareness. The benefits that biosurfactants present like biodegradability, and biocompatibility over their chemical and synthetic counterparts has contributed immensely to their popularity and use in various industries such as petrochemicals, mining, metallurgy, agrochemicals, fertilizers, beverages, cosmetics, etc. With the growing demand for biosurfactants, researchers are looking for low-cost waste materials to use them as substrates, which will lower the manufacturing costs while providing waste management services as an add-on benefit. The use of low-cost substrates will significantly reduce the cost of producing biosurfactants. This paper discusses the use of various feedstocks in the production of biosurfactants, which not only reduces the cost of waste treatment but also provides an opportunity to profit from the sale of the biosurfactant. Furthermore, it includes state-of-the-art information about employing municipal solid waste as a sustainable feedstock for biosurfactant production, which has not been simultaneously covered in many published literatures on biosurfactant production from different feedstocks. It also addresses the myriad of other issues associated with the processing of biosurfactants, as well as the methods used to address these issues and perspectives, which will move society towards cleaner production.

An increasing quantum of pollutants from various industrial sector activities represents a severe menace to environmental & ecological balance. Bioremediation is gaining flow globally due to its cost-effective and environment-friendly nature. Understanding biodegradation mechanisms is of high ecological significance. Application of microbial enzymes has been reported as sustainable approach to mitigate the pollution. Immobilized enzyme catalyzed transformations are getting accelerated attention as potential alternatives to physical and chemical methods. The attention is now also focused on developing novel protein engineering strategies and bioreactor design systems to ameliorate overall biocatalysis and waste treatment further. This paper presents and discusses the most advanced and state of the art scientific & technical developments about biocatalytic remediation of industrial pollutants. It also covers various biocatalysts and the associated sustainable technologies to remediate various pollutants from waste streams. Enzyme production and immobilization in bioreactors have also been discussed. This paper also covers challenges and future research directions in this field., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

This paper presents a comprehensive review of the detection of aflatoxin compounds using carbon allotrope-based sensors. Although aflatoxin M1 and its derivative aflatoxin B1 compounds have been primarily found in milk and other food products, their presence above a threshold concentration causes disastrous health-related anomalies in human beings, such as growth impairment, underweight and even carcinogenic and immunosuppressive effects. Among the many sensors developed to detect the presence of these compounds, the employment of certain carbon allotropes, such as carbon nanotubes (CNTs) and graphene, has been highly preferred due to their enhanced electromechanical properties. These conductive nanomaterials have shown excellent quantitative performance in terms of sensitivity and selectivity for the chosen aflatoxin compounds. This paper elucidates some of the significant examples of the CNTs and graphene-based sensors measuring Aflatoxin M1 (ATM1) and Aflatoxin B1 (AFB1) compounds at low concentrations. The fabrication technique and performance of each of the sensors are shown here, as well as some of the challenges existing with the current sensors.

The aim of this study was to evaluate the effect of raw (RawBC) and iron (Fe)-modified biochar (FeBC) derived from Platanus orientalis Linn branches on the plant growth, enzyme activity, and bioavailability and uptake of As, Cd, and Pb by rice in a paddy soil with continuously flooded (CF) or alternately wet and dry (AWD) irrigation in a pot experiment. Application of RawBC (3%, w/w) significantly increased soil pH, while FeBC decreased it. The FeBC was more effective in reducing As and Pb bioavailability, particularly under the AWD water regime, while RawBC was more conducive in reducing Cd bioavailability under the CF water regime. The FeBC decreased As concentration, but increased concentrations of Cd and Pb in the straw and brown rice, as compared to the untreated soil. Soil catalase and urease activities were enhanced by RawBC, but decreased by FeBC treatment. The FeBC increased the grain yield by 60% and 32% in CF and AWD treatments, respectively. The FeBC can be recommended for immobilization of As in paddy soils, but a potential human health risk from Cd and Pb in FeBC-treated soils should be considered due to increased uptake and translocation of the metals to brown rice., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Sustainable development of circular bioeconomy concept is only possible upon adopting potential advanced technologies for food waste valorization. This approach can simultaneously answer resources and environmental challenges incurred due to capital loss and greenhouse gases accumulation. Food waste valorization opens new horizons of economical growth, bringing waste as an opportunity feedstock for bio processes to synthesize biobased products from biological source in a circular loop. Advanced technologies like Ultrasound assisted extraction, Microwave assisted extraction, bioreactors, enzyme immobilization assisted extraction and their combination mitigates the global concern caused due to mismanagement of food waste. Food waste decomposition to sub-zero level using advanced techniques fabricates food waste into bio-based products like bioactive compounds (antioxidants, pigments, polysaccharides, polyphenols, etc.); biofuels (biodiesel, biomethane, biohydrogen); and bioplastics. This review abridges merits and demerits of various advanced techniques extended for food waste valorization and contribution of food waste in revenue generation as value added products., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Management of food waste (FW) is a global challenge due to increasing population and economic activities. Presently, landfill and incineration are the keyways of FW management, while economical and environmental sustainability have been an issue. Therefore, the biological processes have been investigated for resource and energy recovery from FW. However, these biological approaches have certain drawbacks and cannot be a complete solution for FW management. Therefore, this review aims to offer a detailed and complete analysis of current available technologies to achieve environmental and economical sustainability. In this context, zero solid waste discharge for resource and energy recovery has been put into view. Corresponding to which several innovative technologies using integrated biological methods for resource and energy recovery from FW have been elucidated., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Bio-based rhamnolipid production from waste streams is gaining momentum nowadays because of increasing market demand, huge range of applications and its economic and environment friendly nature. Rhamnolipid type biosurfactants are produced by microorganisms as secondary metabolites and have been used to reduce surface/interfacial tension between two different phases. Biosurfactants have been reported to be used as an alternative to chemical surfactants. Pseudomonas sp. has been frequently used for production of rhamnolipid. Various wastes can be used in production of rhamnolipid. Rhamnolipids are widely used in various industrial applications. The present review provides information about structure and nature of rhamnolipid, production using different waste materials and scale-up of rhamnolipid production. It also provides comprehensive literature on various industrial applications along with perspectives and challenges in this research area., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

This study examines a new method to dispose the biomass of a rare earth elements (REE) hyperaccumulator, Dicranopteris pedata, as a REE containing additive of a basal fertilizer for agricultural application. The D. pedata laminas were calcinated to fabricate ashes. The total REE content was 2.65 % for AshDp500, and 4.12 % for AshDp815, respectively. However, as for the heavy metals, Cd or Pb, a higher content could be found in AshDp500 than in AshDp815. The elemental contents of D. pedata ashes are qualified for fertilizer application. Pot experiments were then conducted to investigate the effects of AshDp815 on both the yield and quality of Ipomoea aquatica Forsskal grown in a yellow brown earth, or in a red soil. The application of the ashes increased the I. aquatica height, biomass, vitamin C, soluble protein, and soluble sugar contents, but decreased the I. aquatica nitrate and free amino acids contents. Furthermore, none of the microelements of I. aquatica leaf exceeded the Chinese national standard. The observations indicate the favorable effect of using D. pedata ash on the growth of I. aquatica which is most likely the result from the fertilizer effects of both macroelements and REE present in the ash., (Copyright © 2020 Elsevier B.V. All rights reserved.)

This work investigated the effect of biochar addition to mitigate VFA accumulation and enhance methane production in mesophilic food waste/sludge co-digestion. Different types of biochar derived from agricultural and forestry residues at two pyrolysis temperatures were tested. Results showed that wheat straw biochar 550 °C supported the highest specific methane yield of 381.9 LCH 4 /kg VS added and VS removal efficiency of 41.62% among all treatments. Degradation of propionic acid and long-chain fatty acids such as valeric, caproic and isovaleric acids was observed. This also corresponded to an increase in methanogenic favorable substrates including acetic acid (>40%) and butyric acid (~20%) over the control. Consequently, a 24% increase in overall methane production was obtained as compared to control. This demonstrated that biochar addition had positive effects on VFA degradation and methane production which could be a useful strategy to increase the organic loading in co-digestions without the fear of process failure., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Indoor mold grows ubiquitously in humid areas and can affect occupants' health. To prevent indoor mold contamination, one of the key measures suggested by the World Health Organisation and United States Environmental Protection Agency is to maintain an indoor relative humidity (RH) level below 75% or at 30-60%, respectively. However, in tropical and subtropical areas, maintaining these suggested RH levels is equivalent to operating a 24-h air-conditioner (AC) or dehumidifier, which is energy-consuming. As a large part of building expense, the operation time of ACs has been regularly proposed to be cut down because of the requirement of building sustainability. This leads to a trade-off between sustainable building performance and indoor mold hygiene. To balance this trade-off, more sustainable alternatives, such as those that target physical environments (e.g. nutrient and temperature level) or apply new surface coating technologies to inhibit mold growth, have been launched. Despite these initiatives, indoor mold contamination remains an unresolved issue, mainly because these alternative measures only exhibit limited effectiveness or require extra effort. This review aims to summarize the currently adopted mold control measures and discuss their limitations as well as the direction for the future development of sustainable mold control strategies. SIGNIFICANCE AND IMPACT OF THE STUDY: People spend most of their time indoors and hence the presence of indoor mold contamination can compromise the occupants' health. With the wake of climate change which is expected to see an increase in RH and temperature, tropical and subtropical areas are even more prone to mold contamination than they used to be. This study may help facilitate the development of sustainable and effective mold control strategies in the indoor environment., (© 2020 The Society for Applied Microbiology.)

In this study, the performance of UASB for treating food waste leachate was investigated, with the objective of studying the effect of conductive material on anaerobic digestion (AD) enhancement at two organic loads. Conductive and control materials (i.e. graphite and glass) were first compared for their surface porosity then dosed in UASB for side-by-side comparison of the corresponding AD performance. In the first phase (organic load of 2660 mg-COD/L), compared to glass-added UASB, 29.5% reduction of effluent COD was observed in graphite-added UASB, however, only a little biogas enhancement (2.3%) was achieved. In the second phase (organic load of 4140 mg-COD/L), the results show that it could promote better AD enhancement in graphite-added UASB, where 36% effluent COD and 38% biogas production enhancement were simultaneously observed. The overall results support that utilization of conductive material is a viable approach for enhancing biogas production in UASB, especially for high organic loads., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier Ltd.)

A membrane-covered composting system was used to investigate the odor emission and microbial community succession during biogas residue composting. Results showed that in comparison with the control (CK) group, the NH 3 and H 2 S emissions outside the membrane of the membrane-covered (CT) group decreased by 58.64% and 38.13%, respectively. The nitrogen preservation rate of the CT group was increased by 17.27% in comparison with the CK group. Moreover, the ammonium nitrogen and nitrate nitrogen contents of the CT group were 37.68% and 11.77% higher than those of the CK group, respectively. Microbial analysis showed that the average abundance and co-occurrence rate of ammonification bacteria dominated by Pseudomonas and Bacillus in the CT group were lower than those in the CK group, and the abundance of anaerobic sulfate-reducing bacteria (SRB) dominated by Desulfovibrio in the CT group was higher than that in the CK group., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Anaerobic hydrolysis of food wastes sourced from bakery (T1), Chinese restaurant (T2), western-style restaurant (T3), and wet market (T4) were performed in leach bed reactors under the scheme of acidogenic off-gas reuse in methanogenic reactor. Results showed that food waste in T3 achieved the highest hydrogen production of 61.0 L/kg·VS added . Highest activity of hydrogenase in both leachate and digestate samples confirmed the superior performance of H 2 production in T3. Mixed acid fermentation with domination of acetate and butyrate was observed in all four treatments, whereas variations in quantification and speciation of the acidogenic products were closely related to the composition of substrates. High volatile solids (VS) removal (76.7%) was observed in T3 while VS reduction rates of the other treatments ranged from 37 to 55%. High COD production of 0.65 gCOD /g·VS added together with the reuse of elevated acidogenic off-gas ensured the highest specific CH 4 production of 0.42 L/g·VS added in T3., (Copyright © 2019. Published by Elsevier Ltd.)

Moisture is one of the critical abiotic factors that can affect mold growth. Indoor humidity is typically fluctuating, which renders a transient water supply for mold growth. Understanding mold growth under water dynamics and its underlying mechanisms can help in the development of novel and sustainable mold prevention strategies. In this study, pre-germination and germinated spores of Cladosporium cladosporioides were exposed to daily wet-dry cycles with different combinations of wetting and drying duration. Afterward, growth delay, cellular H 2 O 2 concentration, and catalase (CAT) activity were measured and compared. We found that under daily wet-dry cycles, the longer the growth delay was observed, the higher the cellular H 2 O 2 concentration was detected, with the 12-12 wet-dry cycle (12-hour wet and 12-hour dry) showing the longest growth delay and highest cellular H 2 O 2 production. A positive correlation between cellular H 2 O 2 concentration and growth delay was suggested by Pearson correlation coefficient and linear regression analysis (P < .0001, R 2  = 0.85). Furthermore, under daily wet-dry cycles, molds derived from pre-germination spores generally exhibited shorter growth delay, lower cellular H 2 O 2 concentration, and higher CAT activity than molds developed from germinated spores. These results together suggest that the growth delay of C. cladosporioides under water dynamics is associated with oxidative stress., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

The aim of this study is to evaluate the effect of leachate recirculation at a ratio of 0%, 25%, 50% or 75% of collected leachate from the Leach Bed Reactor (LBR) on food waste digestion efficiency and its subsequent methane production in the second phase of a two-phase anaerobic system. Higher hydrolysis-acidogenesis efficiency and lower energy loss were achieved in LBR with higher leachate recirculation ratio. Better biochemical balance between metabolic products and microorganisms in leachate was revealed under 50% leachate recirculation ratio, which leads to the highest hydrogen production yield in LBR resulting the highest methane production yield in the corresponding methanogenic phase which was at least 15% higher than that in other conditions. This provides an easy approach to enhance the hydrolysis efficiency and in the same time a biochemical balanced leachate to enhance methanogenic reaction of a two-phase anaerobic digestion., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Background: Sulforaphane (SFN), a natural compound present in cruciferous vegetable, has been shown to possess anti-cancer activities. Cancer stem cell (CSC) in bulk tumor is generally considered as treatment resistant cell and involved in cancer recurrence. The effects of SFN on nasopharyngeal carcinoma (NPC) CSCs have not yet been explored., Purpose: The present study aims to examine the anti-tumor activities of SFN on NPC cells with CSC-like properties and the underlying mechanisms., Methods: NPC cells growing in monolayer culture, CSCs-enriched NPC tumor spheres, and also the NPC nude mice xenograft were used to study the anti-tumor activities of SFN on NPC. The population of cells expressing CSC-associated markers was evaluated using flow cytometry and aldehyde dehydrogenase (ALDH) activity assay. The effect of DNA methyltransferase 1 (DNMT1) on the growth of NPC cells was analyzed by using small interfering RNA (siRNA)-mediated silencing method., Results: SFN was found to inhibit the formation of CSC-enriched NPC tumor spheres and reduce the population of cells with CSC-associated properties (SRY (Sex determining Region Y)-box 2 (SOX2) and ALDH). In the functional study, SFN was found to restore the expression of Wnt inhibitory factor 1 (WIF1) and the effect was accompanied with the downregulation of DNMT1. The functional activities of WIF1 and DNMT1 were confirmed using exogenously added recombinant WIF1 and siRNA knockdown of DNMT1. Moreover, SFN was found to inhibit the in vivo growth of C666-1 cells and enhance the anti-tumor effects of cisplatin., Conclusion: Taken together, we demonstrated that SFN could suppress the growth of NPC cells via the DNMT1/WIF1 axis., (Copyright © 2019 Elsevier GmbH. All rights reserved.)

This work proposes a new treatment approach involving both food waste disposal and sewerage treatment called MOWFAST i.e. Municipal Organic Waste management by combined Food waste disposal and Sewerage Treatment. MOWFAST involves mixing of food waste directly with raw sewage instead of separate addition to sludge and their combined anaerobic digestion (AD). Compared to conventional sludge digestion, MOWFAST exhibited better digestion capability and allowed a greater degradation of organic material along with higher production of methanogenic-favourable products from the beginning of digestion. This resulted in producing higher specific methane yields (7.86 LCH 4 /kg VS added versus 0.95 LCH 4 /kg VS added ) and 1.4-fold higher cumulative methane yield over sludge AD. Furthermore, compared with conventional food waste-sludge co-digestion, MOWFAST gave higher solubilization of organic material (0.82 g sCOD/g VS added versus 0.23 g sCOD/g VS added ) and specific methane yields (7.86 LCH 4 /kg VS added versus 3.2 LCH 4 /kg VS added ). This proves its feasibility for digestion and methane generation potential., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Thermal hydrolysis has proven to be a successful approach to make sewage sludge more amenable to anaerobic digestion. Three heat pretreatment scenarios were compared in this study, i.e. thermal alkaline treatment (LAT, 0.1 M NaOH, 80 °C), low temperature thermal treatment (LT, 80 °C) and high temperature thermal treatment (HT, 170 °C). The biodegradability of pretreated sludge was testified by using biochemical methane potential (BMP) test, meanwhile, the repartition and complexities of organic matters in sludge subjected to various pretreatments were characterized by a revised chemical extraction protocol combined with 3D fluorescence spectroscopy. The cumulative methane yield of sewage sludge was significantly increased by LAT (+135%), LT (+95%) and HT (+112%) as compared to the control. Nevertheless, results show that the solubilization degree of sludge was insignificantly correlated to BMP values, meanwhile high correlation values were observed for the soluble polysaccharide concentration in hydrolysate. The degradation rates of bioaccessible fraction of soluble particulate organic matter (SPOM) and readily extractible organic matter (REOM) were improved after thermal pretreatments at varied levels, which indicates that the chemical accessibility is positively correlated with the bioaccessibility. Furthermore, the biodegradable index F digestion was proposed to evaluate the biodegradability of organic matter, which is helpful for the optimization of various pretreatment strategies., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Food waste (FW) disposal has become a global social, environmental, and economic problem. The current practice of landfilling is undesirable due to its potential emission of greenhouse gas, nutrient recycling, and pollution of water resources. Anaerobic digestion (AD), particularly two-phase AD is a promising option to manage FW and recover energy in the form of methane and obtain value-added by-products. However, most current review literature focuses on operating conditions while often placing little emphasis on improving conversion efficiency through regulating intermediate products. The AD process involves complex metabolic reactions carried out by several microbial groups. Therefore, understanding of these metabolic pathways existing in AD is the key to design effective strategies for enrichment of specific microbial groups which can produce desired intermediates for methane production, which can possibly be achieved by an understanding of the influence of critical process parameters on these metabolic pathways. Thus, it is the aim of this review to describe the effect of process conditions on underlying metabolic pathways in order to allow an efficient manipulation of these pathways for enhancing methane production.

Objective: The role of exercise in preventing or delaying age-related cognitive decline is an important focus of rehabilitation. Tai Chi (TC) is a traditional Chinese exercise that has been found to improve cognitive function. However, the mechanism underlying this improvement is still unknown. We compared the effects of TC practice (mind-body exercise) and arm ergometry (AE; body focused exercise) on prefrontal cortex activity between TC practitioners and non-practitioners., Design: This cross-sectional study included 16 older female subjects (8 TC practitioners and 8 non-practitioners). The practitioners had each practiced TC for at least 7 years. Prefrontal cortex activity was measured using the prefrontal oxygenation level obtained with near-infrared spectroscopy. During the spectroscopy measurement, the participants performed TC, after watching a video of 12-form seated Yang Style TC, and AE in a subsequent session., Results: We found significantly greater changes in the levels of oxyhemoglobin (HbO2; p = 0.022) and total hemoglobin (cHb; p = 0.002) in the TC condition compared with the AE condition in all participants. In the TC practitioner group, a similar trend was shown in the change of HbO2 (p = 0.117) and cHb (p = 0.051) when practicing TC versus AE. However, in the non-practitioner group, we found a statistically greater change in cHb (p = 0.005) but not in HbO2 (p = 0.056)., Conclusion: The older adults had higher brain activity when practicing TC compared with AE, and a significant effect was observed in the non-practitioner group. These pilot results may provide insight into the underlying mechanism of the effectiveness of TC practice in preventing cognitive decline in older adults., (Copyright © 2018. Published by Elsevier Ltd.)