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Ceria, zirconia, Ce0.9Zr0.1O2, Ce0.75Zr0.25O2, and Ce0.5Zr0.5O2 samples were synthesized via the two-step hydrothermal method by using Ce(NO3)3·6H2O and Zr(NO3)4·5H2O as the precursors, respectively. The oxides were applied as catalysts for the dehydrogenation of ethylbenzene by using carbon dioxide as a soft oxidant (CO2-ODEB) at 550 °C and 0.1 MPa. Reaction results indicate that ceria was the most active catalyst while the zirconia with a cubic structure was inactive for CO2-ODEB. In the cases of the oxides by inserting Zr into CeO2, the activity indexed by either the initial ethylbenzene (EB) conversion or the calculated turnover frequency (TOF) at a time on stream of 1 h was changed in the sequence of Ce0.9Zr0.1O2 >> Ce0.75Zr0.25O2 > Ce0.5Zr0.5O2 while the decreasing pattern of the stability was observed in the order of Ce0.5Zr0.5O2 ≈ Ce0.75Zr0.25O2 >> Ce0.9Zr0.1O2. As revealed from the characterization results of XRD and Raman, the pure cubic solid solution of Ce1-xZrxO2 without any phase separations was obtained irrespective of the compositions. By correlating the characterization results, the activity of Ce1-xZrxO2 for CO2-ODEB was determined by the amount of the Ce3+ oxidized by CO2 and the rate of Ce3+/Ce4+ redox cycles. Moreover, both the coking rate and the promoting effect of the deposited coke on CO2-ODEB were responsible for the stability of Ce1-xZrxO2.

An organocatalytic asymmetric arylmethylation/N-hemiacetalization of 2-indolyl methane derivatives and 2-enals was developed. Notably, the 2-methyl of indole was readily deprotonated to produce highly reactive nucleophilic species by introducing the nitro group at the C3 position of the indole ring. A spectrum of valuable chiral dihydropyrido[1,2-a]indoles were efficiently constructed with excellent enantioselectivity (up to >99% ee). Furthermore, the corresponding products could be easily functionalized via simple deprotonation and treatment with other electrophiles with excellent diastereoselectivities (>20:1 dr).

Reactive oxygen species (ROS) are the metabolites of oxygen that plays a significant role in cell signaling and homeostasis. Under normal conditions, ROS formation is stabilized by various antioxidant defense systems (ROS scavengers). Several studies in both in-vitro and in-vivo models, together with clinical data indicated that increased production ROS and oxidative stress plays a crucial role in the development and progression of endothelial dysfunction. The interactions between the main cellular sources of ROS, such as mitochondria and NADPH oxidases, however, remain unclear. The purpose of this review is to outline various sources of ROS and describe the crosstalk between NADPH oxidase and mitochondria. Further, we will discuss different antioxidants that lower ROS production and ROS-induced pathological conditions such as aging, atherosclerosis, diabetes, hypertension, and degenerative neurological disorders. In this review, we have mainly focused on antioxidants that inhibit NADPH oxidase and mitochondrial sources of ROS. Moreover, the modification of antioxidants (targeted therapy) may be a significant approach for management of oxidative stress induced pathophysiological complications.

The research provides a simple method for high-performance electrode preparation for use with a microbial fuel cell and microcurrent to treat pollutants. Blue titania nanotube arrays as a new microbial fuel cell electrode is synthesized by cathodic reduction. The results show that these arrays remarkably increase the electricity generation from 3.64 ± 0.112 mA to 6.246 ± 0.135 mA at the bioanode. A higher carrier concentration of 4.70 × 1024 cm−3, an apparent electron transfer rate constants of 21.69 ms−1 and drastic decrease in resistance to 1352 Ω provide better conditions for electroactive bacteria accumulation. The application of blue titania nanotube arrays to both the anode and cathode results in a photo-microbial fuel cell capable of efficient photoelectrocatalytic degradation of azo dye. The degradation of the azo dye and its intermediate products is attributed to electronic reduction of the dye and oxidation by free radicals.

Cutting force detection can contribute to predicting the productivity and quality of end milling operations. Instantaneous cutting force prediction of digital twins in end milling operations should be near real-time and accurate. This paper proposes an image-based approach that can contain more useful information due to a higher dimension and simplify the complexity of computing geometric data. The cutter frame image (CFI) is utilized as one of the inputs of a convolutional neural network (CNN) to predict instantaneous cutting forces. Considering the convenience of capturing massive data, the approach uses cutting forces generated from a mechanistic force model instead of experimental cutting forces to train the CNN. The correlation coefficient R2 value between predicted results and simulated results is 0.9999 and the average time cost per image is 0.057 s in a cutting condition, which validates the possibility to use the image-based method to predict instantaneous cutting forces accurately and efficiently in the digital twin.

Objective This study aimed to summarize the magnetic resonance imaging (MRI) and computed tomography (CT) features of the central nervous system (CNS) in children with Hoyeraal‐Hreidarsson syndrome. Methods The imaging and clinical data of four children diagnosed with Hoyeraal‐Hreidarsson syndrome by clinical and laboratory tests in the Guangzhou Women and Children's Medical Center were gathered and analyzed retrospectively. The clinical manifestations and CNS imaging features of Hoyeraal‐Hreidarsson syndrome were summarized based on our results and a literature review. Results Our results showed that delayed development, skin pigmentation, nail/toenail dystrophy, thrombocytopenia, and anemia are the most observed clinical presentations of Hoyeraal‐Hreidarsson syndrome. Important findings on CNS imaging showed that all patients had cerebellar hypoplasia, delayed myelination, hydrocephalus, brain atrophy, and calcification. The gene mutations in all cases were consistent with those of dyskeratosis congenita, including TINF2 mutations in three cases and DKC1 mutations in one case. Conclusion Hoyeraal‐Hreidarsson syndrome is a severe variant of dyskeratosis congenita. Both DKC1 and TINF2 mutations can lead to the phenotypes of Hoyeraal‐Hreidarsson syndrome. In our study, CNS imaging revealed that cerebellar hypoplasia has an important diagnostic value for Hoyeraal‐Hreidarsson syndrome while delayed myelination, calcification of the parenchyma, brain atrophy, and hydrocephalus are also important findings on CNS imaging. Combining imaging features with clinical and laboratory indicators can assist the diagnosis of Hoyeraal‐Hreidarsson syndrome., This study aimed to summarize the magnetic resonance imaging (MRI) and computed tomography (CT) features of the central nervous system (CNS) in children with Hoyeraal‐Hreidarsson syndrome. Hoyeraal‐Hreidarsson syndrome is a severe variant of dyskeratosis congenita. Both DKC1 and TINF2 mutations can lead to the phenotypes of Hoyeraal‐Hreidarsson syndrome. In our study, CNS imaging revealed that cerebellar hypoplasia has an important diagnostic value for Hoyeraal‐Hreidarsson syndrome while delayed myelination, calcification of the parenchyma, brain atrophy, and hydrocephalus are also important findings on CNS imaging. Combining imaging features with clinical and laboratory indicators can assist the diagnosis of Hoyeraal‐Hreidarsson syndrome.

Home and community-based services (HCBS) are critical to support our rapidly growing and aging population around the world. This paper takes initial steps to compare HCBS between the USA and Taiwan from four perspectives: funding sources, service types, challenges, and recommendations. Peer-reviewed articles and governmental reports (both English and Chinese) in the U.S.A. and Taiwan were reviewed. Analyses showed both countries mainly use tax dollars to fund HCBS. Although both countries have similar service categories, USA lack a clear organization scheme whereas Taiwan has detailed and clear services provisions. Workforce quality and shortage were common challenges for both countries, especially from culture perceptive. Recommendations for USA include expanded funding pool, better coordination between agencies, and rebalancing HCBS and institutional care with limited budget. Recommendations for Taiwan include expanded service coverage and quality to reduce disparity in rural areas, and providing more support for informal caregivers.

This paper investigates a method to predict the rated output performance of a 1.5kW induction motor accurately. This method uses 2D electromagnetic model to calculate the operating point of the motor. In the model, the results of noload test is used to obtain the magnetic property of the laminations used in the motor, the mechanical and core losses, 3D eddy current field analysis is used to calculate the unbalanced three-phase leakage inductances, and thermal analysis is conducted to determine the conductivities of this motor. Finally, the errors between the calculated results and the data given by the manufacturers are within 5%.

In recent years, tidal current power generation technology has achieved rapid development. On the basis of summarizing the characteristics of tidal current power generation, this paper comprehensively analyzes the distribution law of electric energy in tidal current power generation. Hilbert huang transform was applied to the trend of energy in power quality detection and analysis, the key can power on the trend of the measured data grouping processing, and carried out in accordance with the time period, such as day, month and year of HHT analysis, can get tide power distribution, can verify the HHT analysis in the trend of the power law research in the application feasibility and accuracy.

Soil erosion is the most common form of soil degradation, which is the main problem of soil environmental destruction worldwide. Based on the USLE and GIS remote sensing inversion and superposition analysis technology, this study simulated and calculated soil hydraulic erosion in Fangshan World Geological Park, China in 2011, 2012 and 2013, analyzed its spatial-temporal dynamic changes, and explored the key points of soil erosion control in the study area. The results show that (1) The main erosion occurred at 25°-35° steep slope and 400-800m height, which accounted for more than 30% of the total soil erosion in all the three years. (2) The average erosion modulus and erosion amount in 2012 were significant higher than that in 2011 and 2013 at each slope grade, shown that the soil erosion of 2012 is influenced by natural disaster.With the increase of slope grade, the average modulus also increased.

This study constructed sediment microbial fuel cells (SMFCs) for polycyclic aromatic hydrocarbons (PAHs) removal in contaminated aquaculture sediment. Starch, a waste deposited in aquaculture sediment, was employed as the co-substrate for electricity generation and PAHs removal, and the effect of starch-derived organic acids on SMFC performance was assessed. The results indicated that sufficient starch promoted PAHs removal (69.9% for naphthalene, 55.6% for acenaphthene, and 46.8% for pyrene) in dual-chamber SMFC, whereas excessive starch attenuated SMFC performance because the organic acids accumulation reduced anode pH, decreased species diversity, and changed the microbial communities. The electricity generation and PAHs removal were positively correlated (R 0.96), and both of them were related to Macellibacteroides belonging to Bacteroidetes. However, a larger single-chamber SMFC device did not obtain enhanced PAHs removal owing to the restricted "effective range" of the anode. Hence, more challenges need to be addressed to realize the practical application of SMFC.

Data-driven method has been used to carry out distribution transformer connectivity verification. The imbalanced dataset always results in bad performance of machine learning algorithms. In order to solve this problem, two kinds of over-sampling methods have been studied in this paper. Voltage curves of 3967 distribution transformer which belong to 197 10kV feeders have been collected. The performance of over-sampling methods under different sampling rate has also been studied. Pareto optimality has been carried out in order to obtain the best sampling rate. Results show that with the increase of sampling rate, the value of true negative (TN) increase and the value of true positive (TP) and accuracy decrease. The performance of synthetic minority over-sampling technique (SMOTE) is a little better compared with simple copy method (SCM). When the lower limits of TN, TP and accuracy are set as 0.93, the best sampling rate of SCM is 17 and the values for SMOTE are 16, 17 and 18.

This paper proposes a method to repair the missing voltage data of smart meter users, which includes four steps: acquiring the user voltage data of a power distribution area through the power consumption information acquisition system; calculating the average values of voltage difference at corresponding time between each user and others; making user groups according to the average values of user voltage difference; and repairing the user missing voltage data according to the grouping result. The case study shows that the method can repair the missing voltage data of smart meter users, which provides an important data basis for the development of related business.

Background Human papillomavirus (HPV) vaccine has been increasingly discussed in mainland China since its first approval in 2016. To date, nearly all studies assessing HPV vaccine perceptions and attitudes were implemented during pre-licensure period. Therefore, the nationwide post-marketing survey was conducted to update knowledge, attitudes and practice on HPV vaccine among general population in mainland China. Methods Participants aged 18–45 years living in mainland China were recruited in April 2019 by multi-stage non-randomized sampling. Sociodemographic factors, HPV and HPV vaccine related awareness, knowledge, attitudes, vaccine uptake and potential obstacles were assessed in questionnaires. Bivariate analysis and multivariate regression were used to identify disparity among subgroups with different sociodemographic characteristics. Results 4,000 women (32.1 ± 7.81y) and 1,000 men (31.8 ± 7.96y) were included in final analysis. Less than one third of participants had heard of HPV (female: 31%; male: 22%) and HPV vaccine (female: 34%; male: 23%). Knowledge score was also unfavorable on HPV (female: 3 out of 13; male: 1.8 out of 13) and HPV vaccine (female: 3 out of 6; male: 2 out of 5). Only 3% females had been vaccinated three years after HPV licensure in China, although willingness to get vaccinated among those unvaccinated were high (mean willingness score ± SD: female: 3.3 ± 0.97; male: 3.0 ± 0.98). Industry of employment and household income were the major factors related to awareness and knowledge of vaccine, whereas HPV and HPV vaccine awareness were key influential factors for willingness. The main obstacles of vaccination were safety concerns, lack of knowledge, and high price of HPV vaccines. Conclusions Findings highlight a lack of vaccine awareness, knowledge, and poor uptake in mainland China and underscore the necessity of health education campaigns. The identified priority groups, contents to be delivered and practical obstacles could furthermore provide insight into health education to reduce disparities and accelerate HPV vaccine roll-out in China.

In this study, we constructed a three-chamber microbial fuel cell (TC-MFC) that avoided the adverse effects of H+ diffusion on anode microorganisms in the acidic catholyte and the precipitation of heavy metals in the soil near the cathode side (S4), while also achieving migration of copper from the soil and reduction of Cu2+ in the catholyte. The removal efficiency of acid-soluble Cu from the soil near the anode region reached 42.5% after 63 days of operation at an external resistance of 100 �� and electrode spacing of 10 cm, and Cu2+ in the catholyte was completely removed within 21 days. Heavy metal mobility index (MF) values indicated that the bioavailability and mobility of heavy metals were reduced by the TC-MFC. We found that changing the cathode potential and external circuit current in TC-MFC would affect the type (via XRD) and morphology (via SEM) of cathode deposits and the average removal rate of heavy metals. At the meantime, it should be noted that the interaction between the electric-field-dependent soil heavy metal migration and electron-dependent copper reduction in TC-MFC occurred, which was confirmed to have a relationship with the negative correlation between voltage and current during the test.

As a branch of intercalation type niobium-based anodes for lithium-ion batteries, Nb26Mo4O77 samples are synthesized by hydrothermal and solid-state method, respectively, while the structure and lithium-ion storage characteristics are studied in depth. Nb26Mo4O77 belongs to a monoclinic phase with ordered intergrowth ReO3 structure, which is constructed by a mixture of (Nb/Mo)O6 octahedra blocks of 3 × 4 × ∞ and 4 × 4 × ∞ occurring in alternate sequence and linked by MoO4 tetrahedra, favoring to the structural stability during the rapid lithium-ion deintercalation. Compared to agglomerated Nb26Mo4O77 microparticles synthesized via solid-state process, rod-like nanoparticles synthesized by hydrothermal method with the high specific surface area and reaction reactivity exhibit an improved initial coulombic efficiency (89.3%), notable cyclability (203 mAh g−1 after 503 cycles at 0.5 C), significant intercalation pseudocapacitive contribution (82.2% at 0.9 mV s−1) and increased rate capability (107 mAh g−1 at 10 C). These results provide some new supplements for the improvement of niobium-based anodes.

Benign prostatic hyperplasia (BPH) is commonly treated with 5-alpha-reductase inhibitor/alpha blocker combination therapy or with alpha blocker monotherapy. However, in China, the characteristics of BPH patients receiving 5-alpha-reductase inhibitor/alpha blocker combination therapy or alpha blocker monotherapy remain largely unknown. Therefore, this study compared the characteristics of BPH patients receiving either the 5-alpha-reductase inhibitor finasteride in combination with an alpha blocker or an alpha blocker as monotherapy in clinical practice in China. Data were obtained from a large electronic medical record database from four tertiary hospitals in major cities in China (2009–2016). BPH patients aged ≥ 50 years with ≥ 1 alpha blocker fill on/after the first BPH diagnosis were selected. Patients were further classified as receiving combination therapy (≥ 1 overlapping day of supply for finasteride and an alpha blocker) or alpha blocker monotherapy (did not receive any 5-alpha-reductase inhibitor). Patient characteristics, visit type (in- vs. outpatient) at treatment initiation, and comorbidities were evaluated during the 6-month baseline period and compared between the two groups using two sample t tests and chi-square tests/Fisher’s exact tests. A total of 2666 and 2738 patients received combination therapy and monotherapy, respectively. The combination group was older (70.3 vs. 67.3 years, p

In this study, azo dye decolorization and degradation were realized in the air cathode of constructed wetland-microbial fuel cells (CW-MFCs). Additionally, the relationship between cathode and anode was studied. The cathode diameter was varied from 20 to 30 cm to study the cathode degradation and electricity generation performances, the effects of the cathode on the anode, and the influence of plants in the cathode layer. Different external resistances were applied to study the effects of current intensity on degradation and electricity generation performances. Cathode decolorization performance increased with increasing cathode diameter. Electricity generation performance first increased and then decreased with increasing cathode diameter. The highest decolorization volume (397.64 mg/L) was observed in the CW-MFC with a cathode 25 cm in diameter when the external resistance was 620 Ω. Meanwhile, it had a chemical oxygen demand removal volume of 317.65 mg/L. The highest cathode exchange current density (0.539 A/m2) was observed in the CW-MFC with a cathode 27.5 cm in diameter when the external resistance was 200 Ω. There were more electricity generation generas in the anode layer and there were more anaerobes and amphimicrobes in the larger cathode layer.

Nitrate (NO3−) in wastewater is a rising global threat to ecological and health safety. A sufficient carbon source, as the electron donor, is essential in the conventional biological denitrification process. It is not appropriate to add extra carbon sources into specific water bodies in terms of material cost and secondary pollution. Thus, innovative NO3− removal technologies that are independent of carbon sources, are urgently needed. This study constructed sediment microbial fuel cells (SMFCs) for aerobic denitrification in low-organic matter wastewater and explored the key factors affecting denitrification efficiencies. The SMFC treatments removed 72–91% NO3− through two main denitrifying stages which were driven by carbon sources (COD) and generated electrons, respectively. After COD was fully consumed, denitrification efficiencies were enhanced in SMFC treatments by 24–47% using the generated electrons within 3 days. In this stage, the NO3− removal efficiencies were positively correlated with external current intensities (p < 0.05). The improved denitrification efficiencies were attributed to two enriched phyla in the SMFC cathode. The dominant genera also demonstrated the heterotrophic denitrifying capacity of the SMFC biocathode. Furthermore, electrical characteristics could be used to monitor or regulate the denitrification process in the SMFC system. In conclusion, this study presents an innovative treatment strategy that is economical and eco-friendly compared with conventional physicochemical methods.

Microbial-fuel-cell-coupled constructed wetlands (CW-MFCs) with various cathode layers were used for long-term azo dye wastewater treatment. Their performance was assessed using cathode diameters ranging from 20 to 27.5cm and the influence of plants at the cathode was also examined. Bioelectricity generation, ABRX3 decolorization, and chemical oxygen demand (COD) removal performances first increased and then decreased with increasing cathode diameter. The CW-MFCs with larger cathodes had an anoxic region at the cathode where ABRX3 was decolorized. This phenomenon has not been reported in previous research on MFCs using traditional air cathodes. Anode performance was influenced by the cathode. The CW-MFC with a cathode diameter of 25cm showed the best electrode performance, and the highest voltage and power density were 560mV and 0.88W/m3, respectively. The highest ABRX3 decolorization and COD removal volumes were 271.53mg/L and 312.17mg/L, respectively.

We argue that founder human capital—formal education, same-industry work experience, and prior entrepreneurial experience—will be positively related to the research and development (R&D) search intensity of new technology ventures. In addition, these positive relationships will be strengthened when new technology ventures face an environmental jolt, such as the Great Recession. An empirical analysis of longitudinal data on new technology ventures in the manufacturing industry from the Kauffman Firm Survey (KFS) broadly supports our hypotheses. The results show that all dimensions of founder human capital increase new technology ventures’ R&D search intensity, and the positive effect of same-industry work experience becomes stronger during the Great Recession. However, in contrast to our expectation, the positive effect of prior entrepreneurial experience is weaker during the Great Recession, not stronger. Furthermore, we do not find support for our hypothesis that the positive relationship between founders’ formal education and new technology ventures’ R&D search intensity is stronger during the Great Recession. These seemly contradictory results suggest that although the different dimensions of founder human capital are all positively related to new technology ventures’ R&D search intensity, they exert divergent impacts on it when confronting an environmental jolt. As such, our study extends the situational and institutional determinants of R&D search intensity by focusing on founders’ human capital and provides deeper insights by considering environmental conditions. By shifting the focus from established firms to new ventures, our study also enriches the understanding of new technology ventures’ innovative activities.

The promyelocytic leukemia (PML) gene is a tumor suppressor gene. It was first identified in acute promyelocytic leukemia, in which it is fused to retinoic acid receptor α by the (15;17) chromosomal translocation. The function of the PML protein is frequently lost or aberrant in human solid tumors. In human ovarian carcinoma tissue, PML detected by immunohistochemistry was highly expressed. A PML-silencing vector, pSRG-shPml, was constructed and used to transfect human ovarian cancer cells. Cells were cultured and selected with puromycin for 10-15 days, and then the PML mRNA expression levels were detected by RT-qPCR and immunofluorescence. Proliferation and clone number of PML-depleted cells were detected using MTT assay and colony-forming assay. The protein expression associated with DNA damage and apoptosis was assessed in PML-depleted cells using western blot analysis and immunofluorescence. The results showed that PML was highly expressed in human ovarian tissue. The proliferation and colony formation of ovarian cancer cells were significantly inhibited after PML was depleted. Western blot analysis and immunofluorescence revealed that p-H2AX and cleaved caspase-3 expression significantly increased after PML silencing. PML was located in the nucleus, and it formed foci after X-ray irradiation. PML foci increased significantly with increasing irradiation doses.

Mesenchymal stem cells (MSCs) are considered for potential use as an ideal vehicle to efficiently deliver therapeutic agents in treatment against cancers including melanoma. However, emerging evidence indicates that MSCs promote tumor growth and progression. Therefore, a comprehensive understanding of the role of MSCs is very important to evaluate the MSCs-based therapy in melanoma. B16 melanoma cells treated by MSC conditioned medium (CM), showed significantly enhanced migration and invasion, which was also confirmed in a lung metastasis mice model in vivo. Later, it was found that MSC CM induced an epithelial mesenchymal transition (EMT) in B16 cells. The ELISA assay showed that transforming growth factor-β (TGF-β) was secreted by MSCs and EMT was also induced by recombinant TGF-β in B16 melanoma cells, which suggests the important role of TGF-β in mediating EMT caused by MSC CM. When TGF-β signaling was inhibited by SB431542, the EMT process was significantly eliminated in vitro and in xenograft tumors in vivo. Snail, as a downstream target of TGF-β signaling and an EMT regulator, was upregulated by MSC CM and inhibited by SB431542, which confirms the key role of TGF-β signaling in EMT induced by MSC CM in B16 cells. Taken together, this study shows that MSC induces EMT in melanoma cells in a paracrine manner, which might be mediated by the TGF-β/Snail signaling pathway. Thus, caution should be exercised when considering MSCs-based therapy in melanoma and also in other cancers. Targeting TGF-β signaling and Snail could be further investigated as potential therapeutic approaches for melanoma.

In this study, a microbial fuel cell (MFC)-biofilm electrode reactor (BER) coupled system was established for degradation of the azo dye Reactive Brilliant Red X-3B. In this system, electrical energy generated by the MFC degrades the azo dye in the BER without the need for an external power supply, and the effluent from the BER was used as the inflow for the MFC, with further degradation. The results indicated that the X-3B removal efficiency was 29.87% higher using this coupled system than in a control group. Moreover, a method was developed to prevent voltage reversal in stacked MFCs. Current was the key factor influencing removal efficiency in the BER. The X-3B degradation pathway and the types and transfer processes of intermediate products were further explored in our system coupled with gas chromatography–mass spectrometry.

In this study, biofilm electrode reactors (BERs) were constructed to degrade the azo dye Reactive Brilliant Red (RBR) X-3B. Three different BERs, namely, cathodes with differently structured reactors, cathodes filled with a granular activated carbon (GAC) reactor, and a dimensionally stable anode (DSA) electrode anode reactor, were individually studied to investigate their influence on the removal of both X-3B and chemical oxygen demand (COD). Experimental results showed that it was the internal resistance rather than the total surface area of the cathode that influenced the X-3B removal efficiency in the different cathode reactors. The smaller the internal resistance, the larger the current in the reactor. The larger the current in the whole system, the more electrons that could be utilized for the reduction of X-3B, resulting in higher removal efficiency. The cathode filled with a GAC-BER did not improve the COD removal efficiency, while the X-3B removal efficiency actually declined. Using the DSA electrode as an anode increased the current in the system and improved the removal efficiency. Scanning electron microscopy results showed that the DSA electrode anode had a longer service life than a graphite anode. UV-vis spectral analysis determined that the conjugate system in X-3B was destroyed.

Microbial fuel cells (MFCs) were embedded into constructed wetlands to form microbial fuel cell coupled constructed wetlands (CW-MFCs) and were used for simultaneous azo dye wastewater treatment and bioelectricity generation. For the first time, the effects of different substrate biomass on the performance of CW-MFCs were studied. Group A had the highest substrate biomass of 0.453 g VSS per L, followed in order by group D, group B and group C. CW-MFCs with more substrate biomass showed higher decolorization efficiencies but lower electrode performance. The decolorization efficiency and the maximal power density of group A were 92.7% and 0.117 W m−3, respectively, while the decolorization efficiency and the maximal power density of group C were 76.26% and 0.256 W m−3, respectively. The cathode performance had the greatest impact on the CW-MFC performance. This may be due to the high cathode activation resistance which may be caused by the lack of cathode microbes. The substrate biomass exerted significant effects on the electrode microbes. The CW-MFCs with more substrate biomass had fewer electrode microbes, which may reduce the electrode performance. This study highlights the applications of CW-MFCs and other MFCs that were built in a natural environment.

This paper proposes a method of solving aircraft assembly path planning by sampling search and implemented this method in virtual assembly application. This paper analyzes the advantages of two different sampling search methods, and finally selects the tree sampling search method. The Bullet physics engine is employed to implement obstacle detection. Finally, in the software platform, the simulation of the assembly process is completed based on the OpenSceneGraph visualization engine.

The anode configuration determined the performance of power generation and contaminant removal in microbial fuel cell (MFC). In this study, double anodes were constructed along an up-flow MFC for mitigating the suppression of refractory organic azo dye Reactive Brilliant Red X-3B and increasing the power output. Results revealed that high concentration of X-3B suppressed the power generation of MFC. The maximum power density decreased from 0.413 to 0.161 W/m3, and the inner resistance rose from 448 to 698 Ω. However, double anodes weakened the suppression of X-3B to the current generation. Compared with single anode, the attenuation of MFC current decreased from 48 to 40%. Meanwhile, the X-3B removal efficiency in double-anode MFC was 19.81% higher compared with a single-anode condition when the X-3B was 1000 mg/L. The degradation pathway analysis indicated that aromatic amines formation and further oxidation were achieved sequentially in the MFC. Furthermore, microbial communities in the lower and upper anodes were analyzed, revealing that the microorganisms in the lower anode were more inclined to degrade the pollutant, whereas those in the upper anode were more inclined to generate electricity. This double-anode structure showed the potential for large concentration range of azo dye removal and the current recovery in real textile wastewater.

Purpose Aurora kinase A (AURKA), which belongs to the serine/threonine protein kinase family, has been identified as a key driver of the genesis and progression of diverse tumors. The aim of this study was to determine the clinical significance of AURKA in patients with hepatoblastoma (HB) and the effect of inhibiting AURKA in the HB cell line HuH-6. Methods The expression of AURKA in HB tissue and adjacent normal liver tissue was detected by immunohistochemistry. Then, statistical analysis was performed to evaluate the association between AURKA expression and the clinicopathological characteristics of HB. The effect of AURKA knockdown on cell viability was assessed by CCK-8 assay. EdU and CCK-8 assays, Western blotting, flow cytometry, and transmission electron microscopy (TEM) were used to examine the effect of alisertib (ALS), a selective AURKA small-molecule inhibitor, on the cell cycle, proliferation, apoptosis, and autophagy in HuH-6 human hepatoblastoma cells. Results The expression of AURKA was significantly higher in HB tissue than in adjacent normal tissue. Furthermore, high AURKA expression was associated with advanced Children’s Oncology Group (COG) stage and tumor metastasis of HB. In vitro, AURKA knockdown significantly reduced the viability of HuH-6 cells, while ALS treatment significantly suppressed HuH-6 cell proliferation and induced G1-phase cell cycle arrest by reducing cyclin-D1 expression. Moreover, ALS promoted apoptosis and autophagy by decreasing the activity of p38 MAPK in HuH-6 cells. Conclusion High expression of AURKA is a potential predictor of poor prognosis in HB patients. AURKA knockdown reduced the viability of HuH-6 cells, and ALS treatment inhibited cell proliferation and induced apoptosis and autophagy via the p38 MAPK signaling pathway. Our results suggest that AURKA may be a novel therapeutic target and ALS a potential therapeutic drug for the treatment of HB.

The concept of Digital Twin has been accepted by more and more researchers and enterprises. Many applications of Digital Twin systems are reported such as airplane state monitor and smart factory. In addition, the digital twin is an important measure to achieve intelligent manufacturing and industry 4.0. The advancement of DT system in manufacturing environment is evident. However, current researches in manufacturing domain concentrated on the discussion of architecture optimization of digital twin system combining with existing cyber-physics researches. There are little research works reported practical DT system and discussed the technologies in implementation. Different from current works, this paper proposed an advanced Digital Twin system for CNC machine tool and discussed the critical enablers to make the DT system more practicality.First, data stream from CAD/CAM to CNC is improved with the STEP-NC standard which containing more structured process data to enhance the information in virtual environment to reflect the real machining process. Second, hardware connection method with CNC system is summarized and a uniform interface for data accessing is proposed to simplify the development of DT system. At last, a virtual machining environment (software named "GrapeSim") is presented which contains three functions: estimating the machining state according to the accessed data from CNC; material removing simulation that synchronous with the real machining process to illustrate the geometry shape of machined workpiece; a web server that allowing other devices to obtain the data in virtual environment as a data source of cloud manufacturing.The whole Digital Twin system is tested in COMAC, the manufacturer of ARJ21 and C919 jetliner, and all functions are proved stable and useful.

A palladium-catalyzed conia-ene reaction of 1-alkynylindolin-3-ones has been developed. Three examples of pyrrolo[1,2-a]indoline derivatives were readily obtained with yields up to 82% utilizing this strategy. Importantly, this palladium-catalyzed intramolecular cyclization process represents an important contribution to the application of indole-3-one derivatives.

An organocatalytic asymmetric arylmethylation/

Microbial fuel cells (MFCs) can remove and recover metals in wastewater; however, there are relatively few studies of metal removal from soil by MFCs. In this study, we developed a three-chamber soil MFC consisting of an anode, contaminated soil, and cathode chamber to remove heavy metals from soil. The performance of the soil MFC was investigated by assessing the relationships among current, voltage, and Cu migration, and reduction. The developed soil MFC successfully reduced and removed Cu, and the Cu removal efficiency in the cathode surpassed 90% after only 7 days of operation. External resistance had a remarkable effect on the performance of the soil MFC which was depended on cathodic polarization. The pH in the cathode also depended on the external resistance. Lower external resistance were associated with lower pH values, higher Cu removal efficiencies, and greater amounts removed in the cathode. Based on sequential fractionation, the acid-extractable and reducible fractions were the main fractions that migrated within the three-chamber soil MFC. Enhancing the voltage output in the three-chamber soil MFC by increasing the external resistance promoted Cu migration, enriched Cu near the cathode, and facilitated Cu removal. Therefore, the developed three-chamber soil MFC not only supports heavy metal migration from soil towards the cathode, but can also realize reduction of heavy metals in the cathode by adjusting the current or voltage generated by the soil MFC.

Glioma is the most common primary brain tumor in adults, with high malignancy and poor prognosis. According to the research in these years, the relationship between circular RNAs (circRNAs) and glioma development is abnormally close. Studies on circRNAs in glioma cells revealed that miR-1261 had almost completely matched binding site on the circ-PTPRZ1 sequence, and dual-luciferase reporter gene assay confirmed that circ-PTPRZ1 was a target gene of miR-1261. MiR-1261 inhibited circ-PTPRZ1 expression in glioma cells, while circ-PTPRZ1 did not affect miR-1261 expression. At the same time, circ-PTPRZ1 could promote p-PAK1 expression, while miR-1261 suppressed the activation of PAK1 by regulating the expression of circ-PTPRZ1. Biological behaviors of glioma cells were detected, circ-PTPRZ1 enhanced cell proliferation and invasion, and inhibited cell apoptosis; miR-1261 had the opposite effects, and could terminate the above effects of circ-PTPRZ1. When co-transfected with PAK1 siRNAs and circ-PTPRZ1 over-expression vector, the changes of above biological behaviors were not obvious. Therefore, in glioma cells, the expression of circ-PTPRZ1/PAK1 is regulated by miR-1261, which affects the proliferation, apoptosis, and invasion. This finding provides another powerful evidence for the role of circRNAs in glioma.

Objective: To investigate associations between cardiovascular disease risk factors, including fasting glucose, cholesterol, high density lipoprotein cholesterol (HDL-c), LDL-c, blood pressure, body mass index (BMI), C-peptide, creatinine kinase, smoking, alcohol use, physical activity, C-reactive protein as well as homocysteine levels and cardiovascular events. Methods: Data from 1545 men aged ≥40 years, with testosterone deficiency (TD) (Results: Multivariate logistic regression analysis showed positive associations between TD and BMI (≥35 vs. p = .016), HDL-c (p = .006) and diabetes (diabetes vs. non-diabetes: OR = 1.48, 95% CI: 1.14–1.92, p = .004) as well as negative associations between TD and metabolic equivalent scores (≥12 vs. p = .009) and smoking (Ever vs. never: OR = 0.69, 95% CI: 0.51–0.94, p = .018). Furthermore, total serum testosterone levels were lower in patients with heart failure (p = .04) and angina/angina pectoris (p = .001) compared with subjects without these cardiac problems. Conclusion: Low serum testosterone was associated with multiple risk factors for CHD.

Heavy metal pollution is widespread in the environment. Microbial fuel cell (MFC) technology is effective for heavy metal removal; however, the simultaneous variations of multiple parameters in an MFC make it difficult to fully elucidate the mechanism of heavy metal removal under microcurrent conditions. In this study, we used a potentiostat to control the fixed potential in a simulated MFC system. The effects of pH, potential, and heavy metal concentration on current, heavy metal removal rate, product type, and product morphology were studied through orthogonal testing. The mechanism and significance of each parameter in terms of copper reduction removal were analyzed using tests of between-subjects effects and correlation analysis. We found that: (1) potential did not affect the type of electron acceptor, and substances with high redox potentials preferentially received electrons; (2) copper concentration significantly affected the reduction rate and product particle size of copper by controlling the flux of electroactive substances transferred to the electrode surface; (3) the combined effects of higher pH and copper concentration limited the reduction of copper; (4) current was the most important factor controlling copper reduction, as current strength significantly affected the copper reduction rate and mechanism; and (5) pH and copper concentration significantly affected the current level by altering the ionic strength of the solution.

Since licensure of human papillomavirus (HPV) vaccine in mainland China, little research has been conducted about healthcare providers' (HCPs) understanding and recommendation of HPV vaccine. A multi-stage convenience sample of Chinese HCPs (N = 5270) were surveyed, involving obstetrician-gynecologists, HCPs from Division of Expanded Program on Immunization (DEPI), Community Health Center (CHC) and other non-HPV closely related professions. Binary logistic regression was conducted to explore factors associated with knowledge and recommendation behaviors. Overall, HCPs showed basic HPV/HPV vaccine knowledge with median (interquartile range) score at 9.5 (7.5–11.6) out of 16 and relatively high recommendation behavior (74.8%). Identified knowledge gaps among HCPs included risk factors of HPV infection, best time to vaccinate, prophylactic functions of HPV vaccine and especially classification of low-risk and high-risk types. Profession-specific analysis in individual knowledge item showed HCPs from CHC were suboptimal on HPV while obstetrician-gynecologists were less competent on HPV vaccine knowledge. Obstetrician-gynecologists also recommended vaccination less frequently than HCPs from DEPI and CHC. Besides being key predictors of recommendation practice (2.74, 95% CI: 2.34–3.21), knowledge shared independent determinants with recommendation behavior on age and ethnicity and additionally associated with education and title by itself. Findings highlight overall and profession-specific gaps on HPV and HPV vaccine knowledge and recommendation practice. Future education and training efforts should be profession-niche-targeting and focus much on HCPs with lower title or education background and from minorities.

In order to explore how cutting parameters affect the evolution of subsurface defect of single crystal copper during nanocutting process, a nanocutting model is constructed based on LAMMPS; the nanocutting process of single crystal copper is investigated through molecular dynamics (MD) simulations for different cutting speeds and cutting depths, using EAM potential between Cu atoms and Morse potential between Cu atoms and the tool atoms. The results show that at the same cutting depth, cutting speed does not make much difference to the subsurface dislocation form; at the same cutting speed, cutting depth makes a great difference to the subsurface dislocation form and it also affects the crystal structure type of the defect layer very significantly. Under any cutting parameters, the dislocation form of 1/6 is dominant. With the increase of cutting speed and cutting depth, the dislocation of 1/6 and 1/3 of subsurface layer increased obviously, and the length of dislocation line also increased.

In this study, two double-anode microbial fuel cells (MFCs) were connected in series for degradation of the azo dye reactive brilliant red X-3B. After the series connection, the electricity generation of one of the MFCs decreased, and the other was not affected too much. Due to the special structure in the double-anode MFC reduced the imbalanced performance between the MFC units, the occurrence of voltage reversal was limited. The removal efficiencies in two MFC reactors were not consistent after the series connection, the results showed that the MFC with the reduced electricity generation had the higher removal efficiencies, it was 12.90, 11.66, and 40.05% higher than in the MFC in which the power generation capacity was not affected after the series connection, the MFC without serial connection, and the control group, respectively. Meanwhile, the microbial communities related to the degradation of refractory organic compounds increased and related to electricity generation decreased in the MFC with the reduced electricity generation, the changes of the microbial communities were consistent with its electricity generation and the removal efficiencies. The degradation products in the effluent from two MFC units showed that had the products generated from the MFC with the reduced electricity generation had simpler structures comparing the other MFC unit.