51. Effects of microcystins-LR on genotoxic responses in human intestinal epithelial cells (NCM460)
- Author
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Jihua Chen, Cong Wen, Fei Yang, Xiangling Feng, Yue Yang, Xiaoyan Wang, Xiaoyu Li, and Shuilin Zheng
- Subjects
Cyanobacteria ,Microcystins ,Health, Toxicology and Mutagenesis ,Microcystin ,010501 environmental sciences ,Toxicology ,medicine.disease_cause ,01 natural sciences ,Microbiology ,Cell Line ,03 medical and health sciences ,polycyclic compounds ,medicine ,Humans ,Intestinal Mucosa ,030304 developmental biology ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,0303 health sciences ,biology ,Dose-Response Relationship, Drug ,Toxin ,Epithelial Cells ,Protein phosphatase 2 ,biology.organism_classification ,chemistry ,Marine Toxins ,Genotoxicity ,DNA Damage - Abstract
Microcystin-LR (MC-LR), a cyclic heptapeptide toxin produced by cyanobacteria, was found to induce genotoxic actions in various types of cells. Some investigators reported that microcystin-LR acted as tumor initiator in the observed genotoxic action mediated by this cyanotoxin. However, the underlying mechanisms underlying MC-induced DNA damage in the human intestine epithelium cell line (NCM460) are not known. The purpose of this study was to examine the influence of 24 hr exposure to 5 or 10 µM MC-LR on intestinal DNA damage using NCM460 intestine cell line as a model. Data showed that MC-LR increased Olive tail moment (OTM) as evidenced by the comet assay, inhibited protein phosphatase 2A (PP2A) activity, elevated reactive oxygen species levels (ROS) and enhanced γ-H2AX and
- Published
- 2019