Search

Abstrct: Polycaprolactone/hydroxyapatite (PCL/HA) composite coating was fabricated by a combination of hydrothermal and dipping methods to delay the degradation of Mg alloy AZ31 as bioresorbable materials. The PCL/HA coating was composed of nano rod-shape HA crystals and PCL filled in the space of HA crystals. Compared with the single HA coating, the binding strength between the PCL/HA composite coating and Mg alloy was obviously improved and the PCL/HA coating still adhered to the surface of AZ31 substrate even after 38 days of immersion. The electrochemical corrosion rate of HA coated sample was reduced by ten times after being filled by PCL. The electrochemical impedance spectroscopy (EIS) and immersion test results showed that the PCL/HA composite coating could provide a more effective barrier for Mg substrate than the HA coating alone. The cytocompatibility and the antibacterial property of HA coating and PCL/HA coating were evaluated by culturing with bone marrow-derived mesenchymal stem cells (BMSCs) and methicillin-resistant staphylococcus aureus (MRSA) for 24 h under direct culture conditions, respectively. The PCL/HA composite coating showed better BMSC cell compatibility, more suitable for BMSC adhesion than HA coating alone and showed a potential application prospect as a biological materials. However, from the perspective of clinical applications, the antibacterial property of PCL/HA composite coating needs to be further improved.

In order to explore the low-temperature oxidation characteristics and evolution law of oxidized coal, this paper adopted a temperature-programmed experiment system. Firstly, the low-temperature oxidation temperature-programmed experiment was carried out on the oxidized coal formed by N2 anaerobic cooling after the coal samples were pre-oxidized to 60 ℃, 90 ℃, 120 ℃, 150 ℃, 180 ℃ and 210 ℃ respectively. At the same time, in order to further reveal the characteristics of low-temperature oxidation behavior of oxidized coal formed under different fire extinguishing conditions, the low-temperature oxidation temperature programmed test was carried out on the oxidized coal formed after fire extinguishing with three different volume fractions of N2 when the coal sample was pre-oxidized to 120 ℃. This reveals the changes of oxygen consumption rate, production rate of signature gases (CO, CO2) and heat release intensity of these two kinds of oxidized coals during low temperature oxidation process. The results show that the oxygen consumption rate, signature gas generation rate and heat release intensity of oxidized coal are all lower than those of raw coal. Furthermore, the spontaneous combustion characteristic parameters of coal samples pre-oxidized to 90 ℃ are closer to that of raw coal, indicating that coals pre-oxidized to critical temperature are more likely to reignite. When pre-oxidized to 120 ℃, the higher the volume fraction of N2, the closer the spontaneous combustion characteristic parameters of this oxidized coal are to the original coal, indicating that the coal with higher volume fraction of N2 has stronger recrudescence ability. Therefore, it is necessary to prevent the recurrence of coal when mining the coal body in the near coal seam group, the recrudescence working face and the unsealing fire area.

In this article, an effective finite element method based on dimension reduction scheme is proposed for a fourth-order Steklov eigenvalue problem in a circular domain. By using the Fourier basis function expansion and variable separation technique, the original problem is transformed into a series of radial one-dimensional eigenvalue problems with boundary eigenvalue. Then we introduce essential polar conditions and establish the discrete variational form for each radial one-dimensional eigenvalue problem. Based on the minimax principle and the approximation property of the interpolation operator, we prove the error estimates of approximation eigenvalues. Finally, some numerical experiments are provided, and the numerical results show the efficiency of the proposed algorithm.

Factory-like rearing of silkworm (Bombyx mori) using artificial diet for all instars is a brand-new rearing mode of silkworm. Accurate feeding is one of the core technologies to save cost and increase efficiency in factory silkworm rearing. Automatic identification and counting of silkworm play a key role to realize accurate feeding. In this study, a machine vision system was used to obtain digital images of silkworms during main instars, and an improved Mask R-CNN model was proposed to detect the silkworms and residual artificial diet. The original Mask R-CNN was improved using the noise data of annotations by adding a pixel reweighting strategy and a bounding box fine-tuning strategy to the model frame. A more robust model was trained to improve the detection and segmentation abilities of silkworm and residual feed. Three different data augmentation methods were used to expand the training dataset. The influences of silkworm instars, data augmentation, and the overlap between silkworms on the model performance were evaluated. Then the improved Mask R-CNN was used to detect silkworms and residual feed. The AP50 (Average Precision at IoU=0.5) of the model for silkworm detection and segmentation were 0.790 and 0.795, respectively, and the detection accuracy was 96.83%. The detection and segmentation AP50 of residual feed were 0.641 and 0.653, respectively, and the detection accuracy was 87.71%. The model was deployed on the NVIDIA Jetson AGX Xavier development board with an average detection time of 1.32 s and a maximum detection time of 2.05 s for a image. The computational speed of the improved Mask R-CNN can meet the requirement of real-time detection of the moving unit of the silkworm box on the production line. The model trained by the fifth instar data showed a better performance on test data than the fourth instar model. The brightness enhancement method had the greatest contribution to the model performance as compared to the other data augmentation methods. The overlap between silkworms also negatively affected the performance of the model. This study can provide a core algorithm for the research and development of the accurate feeding information system and feeding device for factory silkworm rearing, which can improve the utilization rate of artificial diet and improve the production and management level of factory silkworm rearing.

Different from the traditional noise control approaches based on sound energy reduction, this study focuses on the annoyance suppression of motor vehicle noise based on audio injection. Firstly, eight motor vehicle noises were selected as the target sounds and three different controllable sounds were used to overlap with different signal-to-noise ratios. Then absolute threshold and combined sound annoyance subjective evaluation experiment were carried out to obtain absolute threshold range and the existence zone of "destructive effect" for combined noise annoyance. Next the influencing factors of the destructive effect are analyzed. The results of the study found that the identifiability of the target sound and the controllable sound is important to the selection of the controllable sound. For most target sounds with high identifiability, the controllable sound with high identifiability and high SNR should be selected. For most target sounds with low identifiability, the controllable sound with low identifiability and low SNR should be selected. Moreover, after the addition of optimal controllable sound, the descend value of the annoyance degree of the target sound with high identifiability is lower than that of the target sound with low identifiability. The results will also provide guidance for the research on audio injection effects, physical mechanisms, the controllable sound selection and optimization design.

Aims: The purpose of this study was to explore a simple and effective method of preparing human acellular amniotic membrane (HAAM) scaffolds, and explore the effect of HAAM scaffolds with juvenile cartilage fragments (JCFs) on osteochondral defects. Methods: HAAM scaffolds were constructed via trypsinization from fresh human amniotic membrane (HAM). The characteristics of the HAAM scaffolds were evaluated by haematoxylin and eosin (H&E) staining, picrosirius red staining, type II collagen immunostaining, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Human amniotic mesenchymal stem cells (hAMSCs) were isolated, and stemness was verified by multilineage differentiation. Then, third-generation (P3) hAMSCs were seeded on the HAAM scaffolds, and phalloidin staining and SEM were used to detect the growth of hAMSCs on the HAAM scaffolds. Osteochondral defects (diameter: 3.5 mm; depth: 3 mm) were created in the right patellar grooves of 20 New Zealand White rabbits. The rabbits were randomly divided into four groups: the control group (n = 5), the HAAM scaffolds group (n = 5), the JCFs group (n = 5), and the HAAM + JCFs group (n = 5). Macroscopic and histological assessments of the regenerated tissue were evaluated to validate the treatment results at 12 weeks. Results: In vitro, the HAAM scaffolds had a network structure and possessed abundant collagen. The HAAM scaffolds had good cytocompatibility, and hAMSCs grew well on the HAAM scaffolds. In vivo, the macroscopic scores of the HAAM + JCFs group were significantly higher than those of the other groups. In addition, histological assessments demonstrated that large amounts of hyaline-like cartilage formed in the osteochondral defects in the HAAM + JCFs group. Integration with surrounding normal cartilage and regeneration of subchondral bone in the HAAM + JCFs group were better than those in the other groups. Conclusion: HAAM scaffolds combined with JCFs promote the regenerative repair of osteochondral defects. Cite this article: Bone Joint Res 2022;11(6):349–361.

Objective The clinical characteristics and imaging information of insular glioma patients were collected to predict mutation status of isocitrate dehydrogenase 1 (IDH1). Methods A total of 596 patients with gliomas confirmed by postoperative pathology in The First Affiliated Hospital with Nanjing Medical University from January 2011 to June 2021 were enrolled, including 72 insular gliomas, 213 frontal gliomas, 165 temporal gliomas, 76 parietal gliomas, 28 and 29 midline gliomas. All patients were examined by MRI, fifteen glioma⁃related features were selected from the visually accessible rembrandt images (VASARI), including enhancement quality, enhancement proportion, non⁃enhancement proportion, necrosis proportion, edema proportion, cyst, thickness of enhanced margin, definition of the enhanced margin, hemorrhage, diffusion, deep white matter involvement, deep ventricle involvement, midline cross, T2⁃FLAIR mismatch, maximum diameter of tumor. Univariate and multivariate Logistic regression analysis were used to screen the predictive factors related to IDH1⁃mutant in insular glioma. The receiver operating characteristic (ROC) curve was plotted and area under the curve (AUC), sensitivity and specificity were calculated to evaluate the predictive power of MRI features for IDH1⁃mutant glioma. Results IDH1 mutation rate was higher in insular and frontal gliomas (P＜0.01, for all). WHO grade Ⅱ had the highest IDH1 mutation rate (P=0.008, 0.000), and grade Ⅳ had the lowest mutation rate (P=0.000). The IDH1 mutation rate in low⁃expression Ki⁃67 gliomas was higher than that in high⁃expression gliomas (P=0.000). Logistic regression analysis showed that weak enhancement (OR=35.671, 95%CI: 2.805-453.600; P=0.006), non⁃enhancement (OR=75.453, 95%CI: 2.881-1872.759; P=0.009), unlimited diffusion (OR=10.573, 95%CI: 1.043-107.175; P=0.046), no deep ventricle involvement (OR=187.601, 95%CI: 2.269-15507.607; P=0.020), T2⁃FLAIR mismatch (OR=47.536, 95%CI: 2.838-796.097; P=0.007) were independent predictive factors for IDH1 mutation in insular glioma. The AUC of enhancement degree, diffusion, deep ventricle involvement and T2⁃FLAIR mismatch for the diagnosis of IDH1⁃mutant glioma were 0.846 (95%CI: 0.748-0.944, P=0.000), 0.730 (95%CI: 0.609-0.850, P=0.001), 0.708 (95%CI: 0.584-0.833, P=0.003) and 0.745 (95%CI: 0.627-0.864, P=0.000). The combination of the 4 groups had the highest diagnostic efficacy, and the AUC was 0.961 (95%CI: 0.923-0.999, P=0.000). Conclusions Low grade insular glioma has a high IDH1 mutation rate. MRI features of weak enhancement and non⁃enhancement, unlimited diffusion, non deep ventricle involvement and T2⁃FLAIR mismatch contribute to noninvasive prediction of IDH1⁃mutant insular glioma.

Optimizing the pigment volume concentration of zinc phosphate pigments can protect Al alloy 6101 from alkaline media. Additionally, zinc phosphate pigments form a shielding film on the substrate and facilitate stopping the penetration of aggressive corrosion ions. The efficiency of eco-friendly zinc phosphate pigments is almost 98% during the corrosion analysis. A comparative study of physical aging of neat epoxy and Zinc Phosphate (ZP) pigment-modified epoxy coatings on Al alloy 6101 was conducted in Xi’an, China, for one year in all four seasons, where in summer for 3 months, results degraded more due to high UV radiation and humidity; it is found that peeling force of ZP pigments modified epoxy coatings is 50% higher of than that of the neat epoxy coatings though both peel-off adhesion strength and scratch test visibility decreased in both coatings; The electrochemical resistance of ZP pigments modified epoxy coatings is about 30% higher of than that of neat epoxy coatings, the corrosion rate of ZP pigments modified epoxy coatings is about 70% lower of than that of neat epoxy coatings, moreover the gloss retention is 20% higher in the modified epoxy; Optical surface observation of the coatings showed that the ZP modified epoxy coating could effectively restrict the crack and shrinkage in coatings after aging experimentation in the natural environment.

In order to improve the prediction accuracy of photovoltaic output power， this paper proposes a prediction method using AFSA （artificial fish swarm algorithm） to optimize BP （back-propagation） neural network. Based on the cleaned data， the paper takes highly correlative meteorological data as input， and photovoltaic output power data as output. It uses the global optimization capabilities and inherent parallel computing capabilities of AFSA to optimize the weights and thresholds of the BP neural network. The photovoltaic output power prediction model based on the AFSA-BP neural network is obtained after training. The simulation analysis of a photovoltaic power station shows that compared with using BP neural networks, genetic algorithm optimized BP neural network， and PSO-BP network， the prediction results of this method are more accurate, the degree of fitting to the original data curve is better， the corresponding error evaluation index is lower, and the training is less time-consuming; the method can rapidly and accurately predict the photovoltaic output power.

7% yttria stabilized zirconia (7YSZ) thermal barrier coatings (TBCs) prepared by air plasma spray were laser-remelted, and subsequently pre-heated and Al2O3 sol-gel repaired for restraining the crack growth in the remelted coatings. The as-prepared coatings were exposed to high temperature molten CaO-MgO-Al2O3-SiO2 (CMAS) to explore their corrosion resistance. The results show that both the laser remelted and the pre-heated, the laser-remelted coatings are densified by CMAS attack. In addition, the thickness of the densified layers is on the same order of that of the non-remelted coatings. Despite of this, the densified layer in the laser remelted coating repaired by Al2O3 sol-gel is much thinner than the other coatings. This indicates that Al2O3 sol-gel repairing coupling with laser-remelted method can effectively improve the CMAS resistance of 7YSZ TBCs due to the refractory anorthite generated during corrosion process. This refractory compound produced between CMAS and Al2O3 sol-gel is capable to decrease the mobility and corrosivity of the CMAS.

Chromium-free materials as eco-friendly coatings with higher corrosion resistance are crucial in various industrial processes. Herein, we report the deposition of cerium-based conversion, a chromium-free, eco-friendly chemical conversion coating for aluminum alloy 6101, by the dip coating method. Immersion in cerium salt precursors assisted with hydrogen peroxide was performed for the deposition of cerium-based conversion coatings on aluminum alloy 6101 at different bathing temperatures. The electrochemical corrosion behavior was assessed in an alkaline solution of sodium hydroxide (pH 11), including mass loss measurements, free corrosion risk, polarization, and electrochemical impedance spectroscopy. X-ray diffraction and photoelectron spectroscopy analysis showed that the coatings were composed of Ce (III) and Ce (IV) oxides. Surface modifications and surface degradation of the coating and substrate after immersion in corrosive media were analyzed by scanning electron microscopy. Additionally, energy dispersive scanning analysis demonstrated the elemental composition before and after corrosion of the cerium salt conversion-based coating. The results demonstrated that selectively deposited cerium-based conversion coatings improved the corrosion resistance by up to 96% in a strong corrosive alkaline media.

In the process of drilling construction, due to the influence of drill pipe weight, geological conditions and drilling technology, the actual drilling trajectory deviation is larger than the design trajectory. The deviation of drilling trajectory leads to the appearance of blank zone in the area covered by gas drainage boreholes, which affects the gas drainage effect. In order to master the distribution of the blank zone of gas pre drainage boreholes, the trajectory of gas pre drainage boreholes is measured by using the borehole trajectory measurement system. After the data processing of the trajectory measurement system, the 3D display of boreholes can be realized, and the offset of boreholes in different directions can be observed from different perspectives of 3D space, which provides data support for the hole filling of the blank zone of boreholes. According to 3d data and 3D results to show the distribution of blank drilling zone, targeted hole filling is carried out to improve the accuracy of gas elimination and ensure the safety of mine production.

Cervical cancer is a type of malignancy that severely threatens women's health. For invasive cervical cancer, standardized preoperative staging and assessment are essential before the start of treatment. With regard toearly cervical cancer, surgery is the major treatment, with considerations for the latest updates in relevant guidelines, and the determination of extent of resection as well as the selection of proper surgical approach. For advanced or relapsed cervical cancer, radiotherapy and chemotherapy are the main treatments. However, there are many challenges and controversies regarding the treatment and clinical research of cervical cancer. We mainly discussed the present status and challenges of cervical cancer diagnosis and treatment based on clinical guidelines and practices, aiming at bettering the real-world practice with the support of clinical guidelines.

Contact characteristic is an important index to characterize the transmission performance of gears. To clarify the contact characteristic of a nutation transmission with double circular-arc spiral bevel gears, this paper carried out a loaded tooth contact analysis by using the finite element method. Firstly, based on the meshing theory, the tooth surface equation of the double circular-arc spiral bevel gear was derived based on which a three-dimensional CAD model of the meshing gear pair was generated. Secondly, a finite element model of the gear pair was developed in Ansys Workbench to simulate contact status of the meshing double circular-arc spiral bevel gear pair. Finally, the influence of load and installation error on the transmission performance of the gear pair was investigated. The results show that a double-point contact occurs on concave and convex tooth surfaces of the double circular-arc spiral bevel gear, and the stress maximum occurs at the toe of bevel gear. With the increase of load, the contact ratio of the meshing gear increases, while the load distribution coefficient decreases, which improves the smoothness of gear transmission. The installation error affects the contact state of the gear pair in that a negative offset error and a positive nutation angle error may degrade the bearing capacity and the transmission stability of the gears. Thus, they should be reasonably controlled in practical applications.

Corn grits were treated with 0.2% sulphurous acid solution. The purpose of this treatment is to protect pure bacteria (yeast, Lactobacillus) from fermentation. Based on the single factor test, the fermentation technique of instant corn noodle was optimized by orthogonal design. The optimum technological parameters were obtained as follows: 0.2% yeast, plus 0.1% lactobacillus bulgaricus, with fermentation time 78 hours, fermentation particle size 8 mm, and fermentation temperature 30 ℃. The product of fermentation was optimized as follows: 3.5% pregelatinized starch with 0.30% sodium hexametaphosphate, 1.0% xanthan gum, and 3% sunflower seed edible oil. The fermentation had the best comprehensive effect on the instant corn noodle with the broken strip rate of 4.5% and cooking loss rate of 4.5%. Through the mechanical hot extrusion of fermented dough, the broken rate of vermicelli was the lowest, which was 4.3%, and the sensory quality score reached 96.6%. The room temperature storage period of vacuum packaging is 14 days, and the cold storage period is 60 days.

Nickel-based single crystal superalloys are widely used for hot components of advanced turbine engines due to their excellent mechanical properties at elevated temperature. Ru is the symbol element of the fourth and fifth generation nickel-based superalloys. During the past decades, many efforts have been made to provide insight into the role of Ru addition in superalloys. In this paper, the research progress in the effect of Ru on the solidification characteristics, as-cast microstructures, precipitation of topologically close-packed (TCP) phases, and creep properties of nickel-based single crystal superalloy was reviewed. The effect of Ru on the solidification characteristics such as solidification path, solidification transition temperature, micro-segregation, and the solidification microstructures such as eutectic, carbide were analyzed systematically. And the reasons why the addition of Ru can inhibit the precipitation of TCP phase and improve the creep property of superalloy were emphatically studied. The composition design and optimization of Ru-containing superalloy are more challenging due to the complexity of the multi-component interaction on microstructure and properties. It was suggested that the future research on Ru-containing superalloys could be explored from the following aspects: the cause of the precipitation of the new Ru-rich phases and the method of inhibiting its precipitation, the effect of Ru addition on solidification defects, the mechanism of the interaction between Ru and other elements on the "reverse partitioning effect" and the precipitation of TCP phases. It could provide significant guidance for the composition optimization and performance improvement of the new Ru-containing nickel-based single crystal superalloys.

Steel tube confined reinforced concrete columns have the advantages of high bearing capacity, good ductility and convenient construction, which meet the requirements of "building industrialization", so they are widely used in high-rise buildings. Based on the plastic-damage constitutive model of concrete and elastic-plastic mixed strengthen constitutive model of steel, finite element software ABAQUS was used to build an elaborate 3D finite element solid model and perform pseudo-static analysis of square steel tube confined RC(STRC) columns. The model considered the confinement effect of the steel tube exerting on the core concrete, as well as reasonable boundary conditions and loading modes. Good agreement was achieved between the finite element analysis results and the existing quasi static experimental results regarding to the failure modes, load-displacement hysteretic curves and load-displacement skeleton curves. Based on the validated model, 136 full scale model examples were established and extensive parametric analysis was conducted. The results show that the confinement of external steel tube can reduce the peak compressive strain and increase the peak compressive stress of concrete, thus delaying the failure of RC column and improving its bearing capacity and ductility effectively. Besides, the failure displacement angle and ultimate bearing capacity increase when the thickness of steel tube increases. Finally, the seismic design method including reasonable thickness and hoop index of the steel tube were proposed for 5 material strength matching and different axial compression ratios. A method to judge the plastic hinge based on concrete strain and steel tube strain was proposed, as well as a practical calculation formula of plastic hinge length. This study can provide basis and support for the seismic design of frame columns.

Noise control technology based on sound energy reduction uses sound pressure level as an evaluation index, and the addition control technology proposed in recent years uses annoyance as an evaluation index. However, no method has been proposed to link the change in sound pressure level with the change in annoyance. In this paper, three types of transformer substation noise are selected as the target sounds, natural sounds and synthetic sounds are selected as the controllable sound, and the pure tone annoyance and combined noise annoyance evaluation experiments are carried out using the standard sample method, and the curve of the decrease value of annoyance and the equivalent sound pressure level change value is obtained. And the effect of audio injection annoyance control is quantitatively analyzed, aiming to build a bridge between addition control and subtraction control. The research results show the effect of controllable sound on the annoyance of substation noise. This study provides new ideas for quantitative analysis of noise control effects and substation noise control.

We used brightfield and epifluorescence microscopy, as well as permeability tests, to investigate the apoplastic histochemical features of plant roots associated with ion hyperaccumulation, invasion, and tolerance of oligotrophic conditions. In hyperaccumulator species with a hypodermis (exodermis absent), ions penetrated the root apex, including the root cap. By contrast, in non-hyperaccumulator species possessing an exodermis, ions did not penetrate the root cap. In vivo, the lignified hypodermis blocked the entry of ions into the cortex, while root exodermis absorbed ions and restricted them to the cortex. The roots of the hyperaccumulators Pteris vittata and Cardamine hupingshanensis, as well as the aquatic invasives Alternanthera philoxeroides, Eichhornia crassipes, and Pistia stratiotes, contained lignin and pectins. These compounds may trap and store ions before hypodermis maturation, facilitating ion hyperaccumulation and retention in the apoplastic spaces of the roots. These apoplastic histochemical features were consistent with certain species-specific characters, including ion hyperaccumulation, invasive behaviors in aquatic environments, or tolerance of oligotrophic conditions. We suggest that apoplastic histochemical features of the root may act as invasion mechanisms, allowing these invasive aquatic plants to outcompete indigenous plants for ions.

With China's strong support for innovation and development, the policies of government guidance funds are an important driving force for leading innovation and economic transformation, and the effect of its related policies is of great significance. This paper selects 518 policies related to government guidance funds in the Beijing–Tianjin–Hebei region from 2005 to 2018 as the research object and constructs a policy efficiency evaluation indicator system from three dimensions of policy intensity, policy objectives and measures. The analytic hierarchy process (AHP) and entropy method are used to weight the output level of policy innovation performance and economic performance of government guidance funds, and grey relational analysis (GRA) is used to measure the relationship between policies of government guidance funds and innovation performance and economic performance, and corresponding policy recommendations based on the research results. The results of the research show that the policy efficiency of the government guidance funds in Tianjin and Hebei is relatively high, and the policy efficiency of the Beijing government guidance funds needs to be improved. In general, the policy efficiency of the government guidance funds needs to be further improved.

The aim of this study was to improve the low accuracy of equipment spare parts requirement predicting, which affects the quality and efficiency of maintenance support, based on the summary and analysis of the existing spare parts requirement predicting research. This article introduces the current latest popular long short-term memory (LSTM) algorithm which has the best effect on time series data processing to equipment spare parts requirement predicting, according to the time series characteristics of spare parts consumption data. A method for predicting the requirement for maintenance spare parts based on the LSTM recurrent neural network is proposed, and the network structure is designed in detail, the realization of network training and network prediction is given. The advantages of particle swarm algorithm are introduced to optimize the network parameters, and actual data of three types of equipment spare parts consumption are used for experiments. The performance comparison of predictive models such as BP neural network, generalized regression neural network, wavelet neural network, and squeeze-and-excitation network prove that the new method is effective and provides an effective method for scientifically predicting the requirement for maintenance spare parts and improving the quality of equipment maintenance.

V-set and transmembrane domain-containing protein 1 (VSTM1) is negatively correlated with inflammation. However, its effect on atherosclerosis (AS) remains largely unexplored. In this study, we aimed to assess the effect of VSTM1 on the biological function of human peripheral blood mononuclear cells /macrophages stimulated by oxidized low-density lipoprotein (ox-LDL).

To explore reasons and solutions for the rancidity of peanut nougat in storage, this paper investigates the oxidation stability of shelled peanuts and unshelled peanut stored in different temperatures and the quality of nougats prepared by adding peanuts treated in different ways. The results show that when raw peanuts are stored at temperatures of 25 ℃, 37 ℃ and 60 ℃, the oxidation rate of shelled raw peanuts is slower than that of unshelled raw peanuts. The peroxide value of shelled peanuts does not change significantly and is stable below 2 mmol/kg, while the peroxide value of roasted cooked peanuts changes faster when stored at 60 ℃, and the change of the shelled peanuts is slower than that of the unshelled peanuts. The oxidation rate of baked shelled peanuts is slower than that of unshelled, which could potentially prolong the shelf life of peanut nougats made of them.

Objective To evaluate the value of CABYR protein and its complex with FSCB in maintaining sperm fertilization ability and investigate the possible mechanism of physiological function of the coruplex by observing the natural fertility of Fscb/Cabyr double gene knockout and Cabyr single gene knockout male mice. Methods Fscb/Cabyr (--/--) double gene knockout male mice, Fscb/Cabyr (++/--) single gene knockout male mice and wild background male mice were mated with wild-type female mice respectively, and the pregnancy and litter production of female mice in each group were observed. The sperm of male mice with different genotypes were labeled with Fluo-4 AM calcium fluorescent probe in vitro and observed under fluorescence microscope, and the level of calcium ion in the sperm were detected by ELISA; The expression of CATSPER channel proteins in sperm of mice with different genotypes was measured by Western blotting. Results All the male mice with Fscb/Cabyr (--/--) double gene knockout and Fscb/Cabyr (++/--) single gene knockout lost their reproductive capacity completely, and failed to make any female mice pregnant and give birth. Compared with the wild-type male mice, the sperm calcium ion concentration of Fscb/Cabyr (--/--) and Fscb/Cabyr (++/--) male mice was decreased significantly (P < 0.000 1); The expression of CATSPER-1 protein in the sperm of Fscb/Cabyr(--/--) mice was decreased obviously. The expression of CATSPER-4 in the sperm of Fscb/Cabyr(--/--) and Fscb/Cabyr(++/--) mice were notably decreased. Conclusion The deletion of either CABYR or FSCB/CABYR protein complex in male sperm leads to the loss of reproductive function in the male mice, which may be realized by affecting the expression of calcium channel proteins CATSPER-1 and CATSPER-4, concentration of calcium ions in sperm, and then motility function of sperm.

MicroRNAs (miRNAs) operate as tumor suppressor or carcinogen to regulate cell proliferation, metastasis, invasion, differentiation, apoptosis, and metabolic process. In the present research, we investigated the effect and mechanism of miR-496 in human gastric cancer cells. miR-496 was downregulated in two gastric cancer cell lines, AGS and MKN45, compared with normal gastric epithelial cell line GES-1. miR-496 mimics inhibited the proliferation of AGS cells after the transfection for 48 and 72 h. The migration and invasion of AGS cells were also inhibited by the transfection of miR-496 mimics. miR-496 mimics induced the apoptosis through upregulating the levels of Bax and Active Caspase 3 and downregulating the levels of Bcl-2 and Total Caspase 3. Bioinformatics analysis showed that there was a binding site between miR-496 and Lyn kinase (LYN). miR-496 mimics could inhibit the expression of LYN in AGS cells. LYN overexpression blocked the inhibition of tumor cell growth, as well as the inhibition of AKT/mTOR signaling pathway induced by miR-496. In conclusion, miR-496 inhibited the proliferation through the AKT/mTOR signaling pathway via targeting LYN in gastric cancer cells. Our research provides a new potential target for clinical diagnosis and targeted treatment for gastric cancer.

The manipulator is the key component of the anchor drilling robot to automatically complete the anchoring operation underground. Due to the complexity of its motion equation and the limitations of its control strategy, the real-time pose and the positioning accuracy of the manipulator are inferior, which seriously restricts the safety, efficiency, and speed of roadway excavation. In order to improve the positioning accuracy and realize the optimal efficiency of the manipulator, this article designs a manipulator structure with four degrees of freedom. With the help of the D-H method and the intelligent parameter setting method, this article carries out the basic theoretical research on the kinematics and the fractional order FOPID control algorithm of the manipulator of the mining roof bolter, and formulates a manipulator motion control strategy. At the same time, combined with numerical simulations and field experiments, we explore the robustness and control efficiency of the hydraulic system of the manipulator under the working conditions of a harsh environment and limited space, and reveal that the intelligent optimization algorithm can control the motion state of the manipulator more accurately and stably after the parameters of the fractional order FOPID controller are positively determined. This study effectively solved the dynamic model uncertainty caused by time-varying internal parameters and external loads of the hydraulic servo system, optimized and reconstructed the structure and motion coefficient parameters of the manipulator, and revealed the control mechanism of a precise spatial positioning and online trajectory planning of the hydraulic servo system of the manipulator. Compared with the traditional PID control algorithm, this algorithm has a faster response speed and better expected track tracking ability. This research lays a theoretical foundation for the precise positioning and automatic support of the manipulator, and also provides a reference for the design of similar motion control algorithms.

Objective To investigate the effects of preoperative use of metformin on the biochemical recurrence (BR) in the patients with localized prostate cancer after radical prostatectomy (RP) and on the occurrence of castration resistance in patients with bone metastasis after RP. Methods A retrospective analysis was performed on 192 patients diagnosed with localized prostate cancer and undergoing RP in our hospital from January 2008 to April 2020, including 31 cases of postoperative BR and 161 cases of bone metastatic prostate cancer. The patients were further divided into observation group (metformin with/without androgen deprivation therapy) and control group (no treatment/androgen deprivation therapy alone), based on whether they were preoperatively treated with metformin or not. The baseline characteristics of the 2 groups were collected and compared, including age, initial prostate specific antigen (PSA) level, PSA level at BR, pathological T stage, and Gleason score. The time to BR or to progression to castration-resistant prostate cancer (CRPC), PSA doubling time (PSADT), and PSA minimum were calculated and analyzed between the 2 groups. Results For the postoperative BR patients, the median time to BR (22.73 vs 10.73 months, P < 0.05) and mean PSADT (4.69 vs 4.30 months, P < 0.05) were significantly longer in the observation group than the control group. For the patients with bone metastatic prostate cancer, the observation group had obviously longer median time to CRPC (22.35 vs 16.05 months, P < 0.05) and median PSADT (4.50 vs 2.60 months, P < 0.05) when compared with the control group. But no such difference was seen in PSA minimum after treatment between them. Conclusion Metformin can effectively delay the progression of localized prostate cancer to BR after RP, and the progression of bone metastatic prostate cancer to CRPC.

Abstract Large-scale use of electric vehicles will greatly increase the traffic pressure on urban road network. Therefore, planning of charging stations for electric vehicles considering charging demand and transportation network is particularly important for the coordinated development of electric vehicles and intelligent transportation. Under the condition of bounded rationality, this paper considers such factors as the travel utility perception difference between the users of fuel vehicles and electric vehicles, the time-varying of traffic flow, the location and service level of charging stations. On this basis, combining the cumulative prospect theory, dynamic traffic flow allocation and charging demands, a two-level programming model is established to solve the problem of charging station site selection. The upper layer is a system optimal model, the goal is to minimize the travel time of the network. The lower model describes the time-variability of departure time and the randomness of charging and travel behaviors, establishes the dynamic user equilibrium model and designs the heuristic algorithm. The validity of the model and algorithm is verified by a numerical example. Through the simulation experiment, the optimal location scheme of charging station under different electric vehicle proportion is obtained, and the driving characteristics of two types of vehicles are analyzed. Compared with the traditional model, it is found that the charging station planning considering bounded rationality can achieve higher road network traffic efficiency with fewer charging piles.

Power side-channel attacks, have become a serious threat to embedded computing devices in cyber-physical systems because of the ability of deducing secret data using statistical analysis. A common strategy for designing countermeasures against power-analysis-based side-channel attacks uses random masking techniques to remove the statistical dependency between secret data and side-channel information. Although existing techniques can verify whether a piece of cryptographic software code is perfectly masked, they are limited in accuracy and scalability. In order to eliminate such limitations, a refinement-based method for verifying masking countermeasures is proposed. This method is more accurate than prior type-inference based approaches and more scalable than prior model-counting based approaches using satisfiability (SAT) or satisfiability modulo theories (SMT) solvers. Indeed, this method uses a set of semantic type-inference rules to reason about distribution type. These rules are kept abstract initially to allow fast deduction, and then specified when the abstract version is not able to resolve the verification problem. This method is implemented in a software tool called SCVerify and is evaluated on cryptographic benchmarks including advanced encryption standard (AES) and message authentication code Keccak (MAC-Keccak). The experimental results show that the method significantly outperforms state-of-the-art techniques in terms of accuracy and scalability.

The drilling tracker and hand held tracker measurement software for underground rotary borehole in coal mines has problems such as single function, counterintuitive display, inability to analyze and display the blind area of gas extraction and inability to guide the construction of boreholes. In order to solve the above problems, a borehole data processing and 3D display software is designed. The software includes four functional modules, including borehole data pre-processing, borehole depth and track calculation, borehole track 3D modeling and display and borehole track design guidance. The borehole data pre-processing module focuses on the calculation of geomagnetic declination and the elimination of data outliers. The borehole depth and track calculation module uses the borehole water pressure monitoring data combined with the measured point inclination data to realize the extraction of borehole hydrostatic pressure and the calculation of borehole depth. Moreover, the module obtains the calculation of borehole track and the extraction of borehole measuring point coordinates so as to complete the deep-level data mining of the borehole track. Borehole track 3D modeling and display module presents borehole information such as borehole track and coal seam direction through 3D entities effectively, which facilitates the determination of the blind area for gas extraction from borehole. The module includes the 3D display of borehole group track and coal seam distribution, the coverage area of designed borehole in the coal face, and the coverage area of actual borehole in the coal face. The borehole track design guidance module provides the blind area of the borehole extraction, calculate the offset characteristics of the existing borehole track, and provides the guidance track of the subsequent supplementary borehole construction. The software is used to perform 3D display and offset law analysis on the actual borehole of a mine, and the drilling site is supplemented by drilling with precision drilling technology and track measurement technology. The extraction coverage area of the boreholes after supplementary drilling reaches more than 95% of the designed coverage area. The module controls or eliminates the blind area of gas extraction in boreholes effectively. The software solves the problem of 3D display of the track of the drilling site or roadway borehole group, reproduces the relationship between the borehole trajectory and the coal seam visually, and provides a new method for quantitative evaluation of the blind area of gas extraction in boreholes.

Myricaria laxiflora (Tamaricaceae) is an endangered plant that is narrowly distributed in the riparian zone of the Three Gorges, along the Yangtze River, China. Using bright-field and epifluorescence microscopy, we investigated the anatomical and histochemical features that allow this species to tolerate both submerged and terrestrial environments. The adventitious roots of Myr. laxiflora had an endodermis with Casparian bands and suberin lamellae; the cortex and hypodermal walls had lignified thickenings in the primary structure. In the mature roots, the secondary structure had cork. The apoplastic barriers in stems consisted of a lignified fiber ring and a cuticle at the young stage and cork at the mature stage. The leaves had two layers of palisade tissue, a hyaline epidermis, sunken stomata, and a thick, papillose cuticle. Aerenchyma presented in the roots and shoots. Several Myr. laxiflora structures, including aerenchyma, apoplastic barriers in the roots and shoots, were adapted to riparian habitats. In addition, shoots had typical xerophyte features, including small leaves, bilayer palisade tissues, sunken stomata, a thick, papillose cuticle, and a hyaline epidermis. Thus, our study identified several anatomical features that may permit Myr. laxiflora to thrive in the riparian zone of the Three Gorges, China.