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Your search keyword '"Zheng, Hailing"' showing total 74 results
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74 results on '"Zheng, Hailing"'

52. Development of Immunochromatographic Strip Assays Based on a Tailored Monoclonal Antibody for the on-Site Characterization of Ancient Silk.

Author

Li, Qingqing, Zhu, Chengyu, Deng, Bozhi, Ma, Weiwei, Zheng, Hailing, Zhou, Yang, Peng, Zhiqin, Hu, Zhiwen, and Wang, Bing

Subjects
COLLOIDAL gold, PAPER chromatography, SILK fibroin, SILK, MONOCLONAL antibodies, COLLOIDS
Abstract

The characterization of ancient silk provides significant historical information and contribute to the identification of the origin of the material. At present, there are many ways to characterize silk. However, on-site identification of ancient silk remains a crucial challenge. In this study, a paper chromatography system consisting of a colloidal gold immunochromatographic strip (GICS) and a time-resolved fluorescence immunochromatographic strip (TICS) is proposed to meet this challenge. On the basis of the original paper chromatography technique, a monoclonal antibody against silk fibroin (SF) was prepared and applied in this new paper chromatography system. Both the GICS and the TICS showed good sensitivity, specificity and reliability, with the limit of detection for silk fibroin being 30 μg/mL and 49.20 ng/mL, respectively. Moreover, there was no cross reaction with possible interferents. Furthermore, the GICS had acceptable thermal stability. The recovery of the TICS assay was in the range from 98.12% to 103%, and the variation coefficient was from 5.34% to 6.93%. This work significantly improves access to scientific information at archaeological sites, providing new scientific evidence for exploring the origin and transmission roadmap of ancient silk. [ABSTRACT FROM AUTHOR]

Published
2021
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63. An adaptive detection method for fetal chromosomal aneuploidy using cell-free DNA from 447 Korean women

Author

Kim, Sunshin, primary, Jung, HeeJung, additional, Han, Sung Hee, additional, Lee, SeungJae, additional, Kwon, JeongSub, additional, Kim, Min Gyun, additional, Chu, Hyungsik, additional, Han, Kyudong, additional, Kwak, Hwanjong, additional, Park, Sunghoon, additional, Joo, Hee Jae, additional, An, Minae, additional, Ha, Jungsu, additional, Lee, Kyusang, additional, Kim, Byung Chul, additional, Zheng, Hailing, additional, Zhu, Xinqiang, additional, Chen, Hongliang, additional, and Bhak, Jong, additional

Published
2016
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67. Species Identification of Silks from Bombyx mori, EriSilkworm and Chestnut Silkworm Using Western Blot and Proteomics Analyses

Author

Gu, Jincui, Xu, Chengfeng, Li, Menglu, Chen, Boyi, Shang, Yating, Zheng, Hailing, Zhou, Yang, Hu, Zhiwen, Peng, Zhiqin, and Wang, Bing

Abstract

Species identification is of key significance for exploring the origin and transmission of ancient silks. In this study, two novel methods, i.e.western blot (WB) and proteomics analyses, were proposed and established to identify the differences between silks from Bombyx mori(B. mori) and two other distinctive species (Eri silkworm and Chestnut silkworm). Three diagnostic antibodies, a polyclonal anti-silk fibroin (anti-SF) antibody (pAb), a polyclonal anti-SF-specific peptide antibody (pAsb), and a monoclonal anti-SF antibody (mAb) were designed and prepared to distinguish silk species using the antibody-based WB technique. Proteomics analysis by liquid chromatography–tandem mass spectrometry was performed to further identify silk species at the protein level. WB results indicated that the three antibodies showed high specificity and affinity and could discern B. morisilk from Eriand Chestnutsilks. Biomarkers for each SF were obtained using proteomics analysis, and they have the potential to serve as standards for identifying silk species. Thus, combining WB and proteomics analyses with conventional methods can provide more accurate silk information and may be suitable for identifying other proteinaceous materials in archaeological field.

Published
2019
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71. The Feasibility Study of Non-Invasive Fetal Trisomy 18 and 21 Detection with Semiconductor Sequencing Platform

Author

Jeon, Young Joo, primary, Zhou, Yulin, additional, Li, Yihan, additional, Guo, Qiwei, additional, Chen, Jinchun, additional, Quan, Shengmao, additional, Zhang, Ahong, additional, Zheng, Hailing, additional, Zhu, Xingqiang, additional, Lin, Jin, additional, Xu, Huan, additional, Wu, Ayang, additional, Park, Sin-Gi, additional, Kim, Byung Chul, additional, Joo, Hee Jae, additional, Chen, Hongliang, additional, and Bhak, Jong, additional

Published
2014
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72. The aging effect of CaCl2 and NaCl on wool fabrics with hydrothermal treatment.

Author

Lin, Zhen, Yu, Ning, Zhou, Yang, Zheng, Hailing, Wan, Junmin, Wang, Bing, Peng, Zhiqin, and Hu, Zhiwen

Subjects
WOOL textiles, PRESERVATION of textiles, SCANNING electron microscopy, CALCIUM chloride, AGING of textiles, TEXTILE chemicals
Abstract

To conduct research into the long-term preservation of fragile wool textile relics unearthed at archeological sites, it is essential to simulate the aging process and evaluate the factors affecting the degradation of wool fabrics. An accelerated aging method is therefore proposed to simulate the degradation process using either CaCl2 or NaCl in conjunction with hydrothermal treatment. The accelerated aging of wool fabrics was investigated by color measurement, scanning electron microscopy, cross-sectional observation, attenuated total reflection Fourier transform infrared spectroscopy, and amino acid analysis. Wool fabrics subjected to NaCl-hydrothermal or CaCl2-hydrothermal treatment exhibit an apparent yellowing with increasing treatment time. Fluctuations of cysteic acid and cystine dioxide content are shown to be the most prominent, and a distinct conversion of α-helices into β-sheets is observed with increasing treatment time. These results indicate that the effect of Ca2+ was greater than Na+ in promoting the degradation of disulfide groups in the hydrothermal degradation process. [ABSTRACT FROM AUTHOR]

Published
2017
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73. Articles on Comparative Literature in China

Author

Yue, Daiyun, primary, Fang, Hanwen, additional, Zhang, Hong, additional, Cheng, Guangwei, additional, Li, Xiaojun, additional, Zheng, Hailing, additional, Song, BingHui, additional, Yang, Lixin, additional, Chen, Xiaolan, additional, Lei, Na, additional, and Yan, Jindong, additional

Published
2005
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