65 results on '"Zhou, Juhua"'
Search Results
52. Cutting Edge: Persistence of Transferred Lymphocyte Clonotypes Correlates with Cancer Regression in Patients Receiving Cell Transfer Therapy
- Author
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Robbins, Paul F., primary, Dudley, Mark E., additional, Wunderlich, John, additional, El-Gamil, Mona, additional, Li, Yong F., additional, Zhou, Juhua, additional, Huang, Jianping, additional, Powell, Daniel J., additional, and Rosenberg, Steven A., additional
- Published
- 2004
- Full Text
- View/download PDF
53. Down-regulation of RNA Helicase II/Gu Results in the Depletion of 18 and 28 S rRNAs in Xenopus Oocyte
- Author
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Yang, Hushan, primary, Zhou, Juhua, additional, Ochs, Robert L., additional, Henning, Dale, additional, Jin, Runyan, additional, and Valdez, Benigno C., additional
- Published
- 2003
- Full Text
- View/download PDF
54. Identification of a Mutated Fibronectin As a Tumor Antigen Recognized by CD4+T Cells
- Author
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Wang, Helen Y., primary, Zhou, Juhua, additional, Zhu, Kuichun, additional, Riker, Adam I., additional, Marincola, Francesco M., additional, and Wang, Rong-Fu, additional
- Published
- 2002
- Full Text
- View/download PDF
55. Maize myosins: Diversity, localization, and function
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Liu, Liyun, primary, Zhou, Juhua, additional, and Pesacreta, Thomas C., additional
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- 2001
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56. Cytokine-independent growth and clonal expansion of a primary human CD8+T-cell clone following retroviral transduction with the IL-15 gene
- Author
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Hsu, Cary, Jones, Stephanie A., Cohen, Cyrille J., Zheng, Zhili, Kerstann, Keith, Zhou, Juhua, Robbins, Paul F., Peng, Peter D., Shen, Xinglei, Gomes, Theotonius J., Dunbar, Cynthia E., Munroe, David J., Stewart, Claudia, Cornetta, Kenneth, Wangsa, Danny, Ried, Thomas, Rosenberg, Steven A., and Morgan, Richard A.
- Abstract
Malignancies arising from retrovirally transduced hematopoietic stem cells have been reported in animal models and human gene therapy trials. Whether mature lymphocytes are susceptible to insertional mutagenesis is unknown. We have characterized a primary human CD8+T-cell clone, which exhibited logarithmic ex vivo growth in the absence of exogenous cytokine support for more than 1 year after transduction with a murine leukemia virus–based vector encoding the T-cell growth factor IL-15. Phenotypically, the clone was CD28−, CD45RA−, CD45RO+, and CD62L−, a profile consistent with effector memory T lymphocytes. After gene transfer with tumor-antigen–specific T-cell receptors, the clone secreted IFN-γ upon encountering tumor targets, providing further evidence that they derived from mature lymphocytes. Gene-expression analyses revealed no evidence of insertional activation of genes flanking the retroviral insertion sites. The clone exhibited constitutive telomerase activity, and the presence of autocrine loop was suggested by impaired cell proliferation following knockdown of IL-15Rα expression. The generation of this cell line suggests that nonphysiologic expression of IL-15 can result in the long-term in vitro growth of mature human T lymphocytes. The cytokine-independent growth of this line was a rare event that has not been observed in other IL-15 vector transduction experiments or with any other integrating vector system. It does not appear that the retroviral vector integration sites played a role in the continuous growth of this cell clone, but this remains under investigation.
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- 2007
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57. A Probe into the Mentality of Sixty-five Rural Young Women Giving Birth to Baby Girls
- Author
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Zhou Juhua
- Subjects
History ,Sociology and Political Science ,Anthropology ,media_common.quotation_subject ,Birth date ,Begging ,Gender studies ,Girl ,Rural area ,Rural women ,Indignation ,media_common - Abstract
Last year when I was at Qidong, I heard that a village woman left her newborn baby girl by the side of the public restroom. This year I personally saw in Qiyang a resident find a girl infant at his doorstep when he opened the door in the morning. Tied to the infant was a slip of red cloth on which was written the infant's birth date and a message begging other people to adopt the child, as the father would not accept the baby girl and the mother had no choice but to abandon her. This has aroused my indignation and provoked my thinking. I felt the necessity to visit the countryside to probe into rural women's mentality regarding the bearing of baby girls in an attempt to answer the following questions: 1. What is the proportion of rural women who are unwilling to give birth to baby girls?
- Published
- 1988
58. Expression, regulation and function of MicroRNAs in endometriosis.
- Author
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Mu P, Zhou J, Ma X, Zhang G, and Li Y
- Subjects
- Biomarkers, Endometriosis diagnosis, Female, Gene Expression Regulation, Humans, MicroRNAs analysis, MicroRNAs genetics, RNA Interference, Endometriosis genetics, MicroRNAs biosynthesis
- Abstract
Endometriosis (EMS), characterized by the presence and growth of functional en do met rial-like tissues outside the uterine cavity, is a common and benign gyneco logical disorder with a poorly understood and somewhat enigmatic etiopathogenesis and pathophysiology. MicroRNAs (miRNAs) are single-stranded 19-25 nucleotide-long RNAs and have an important role in post-transcriptional gene silencing by base pairing with target mRNAs. Recent research has shown that miRNAs and their target mRNAs are differentially expressed in endometriosis and other disorders of the female reproductive system. In this paper, we review the recent progress in understanding the roles of miRNAs in endometriosis, and specific miRNAs as biomarkers and therapeutic targets for endometriosis.
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- 2016
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59. Successful surgical repair of central slip rupture in finger extensor tendon.
- Author
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Hou Z, Zhao L, Yu S, Xiao B, and Zhou J
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, Rupture, Treatment Outcome, Fingers pathology, Tendon Injuries pathology, Tendon Injuries surgery
- Abstract
Unlabelled: The aim of the present study was to develop a novel effective surgical method to repair boutonniere deformity resulting from central slip rupture of finger extensor tendon. Currently, there are not standard methods available to treat boutonniere deformity., Patients and Methods: In the current study, 8 patients with boutonniere deformity on the left hand were included. All patients had a central slip rupture of the ring fingers. Autologous palmaris longus tendon was used to surgically repair the central slip rupture., Results: We successfully used the autologous palmaris longus tendons in the effective treatment of central slip rupture of the ring fingers resulting in boutonniere deformity on the left hands., Conclusion: To our knowledge, our study was the first illustration for a successful surgical repair of boutonniere deformity which resulted from central slip rupture, using autologous palmaris longus tendon. This method provides an alternative approach for the effective treatment of boutonniere deformity., (Copyright © 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)
- Published
- 2014
60. Antitumor activity of total flavonoids from Tetrastigma hemsleyanum Diels et Gilg is associated with the inhibition of regulatory T cells in mice.
- Author
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Feng Z, Hao W, Lin X, Fan D, and Zhou J
- Abstract
Objective: To determine the antitumor activity of Radix tetrastigmae flavonoids and their inhibitory effect on regulatory T cells (Tregs) in mice., Materials and Methods: Total flavonoids were isolated from Radix tetrastigmae, the root of Tetrastigma hemsleyanum Diels et Gilg, and administered to C57BL/6 mice by oral gavage after inoculation with Lewis lung carcinoma (LLC) cells. The effects of total flavonoids on tumor growth in vivo were examined. Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration., Results: Total flavonoids from T. hemsleyanum Diels et Gilg significantly inhibited tumor growth in C57BL/6 mice inoculated with LLCs. These flavonoids dramatically suppressed regulatory T-cell development in tumor-bearing mice. Further studies revealed that total flavonoids significantly decreased the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 in tumor-bearing mice, which may be responsible for the inhibition of Tregs., Conclusion: The antitumor activity of total flavonoids from T. hemsleyanum Diels et Gilg is associated with the inhibition of Tregs in a mouse tumor model. Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.
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- 2014
- Full Text
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61. Role of cytokines as a double-edged sword in sepsis.
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Chaudhry H, Zhou J, Zhong Y, Ali MM, McGuire F, Nagarkatti PS, and Nagarkatti M
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- Animals, Biomarkers blood, Genetic Predisposition to Disease, Humans, Inflammation Mediators physiology, Polymorphism, Single Nucleotide, Sepsis genetics, Sepsis therapy, Signal Transduction, Cytokines physiology, Sepsis immunology
- Abstract
Background: Sepsis is a deadly immunological disorder and its pathophysiology is still poorly understood. We aimed to determine if specific pro-inflammatory and anti-inflammatory cytokines can be used as diagnostic and therapeutic targets for sepsis., Materials and Methods: Recent publications in the MEDLINE database were searched for articles regarding the clinical significance of inflammatory cytokines in sepsis., Results: In response to pathogen infection, pro-inflammatory cytokines [interleukin-6 (IL-6), IL-8, IL-18 and tumor necrosis factor-α (TNF-α)] and anti-inflammatory cytokine (IL-10) increased in patients with sepsis. Importantly, a decrease in IL-6 was associated with a better prognosis and overproduction of IL-10 was found to be the main predictor of severity and fatal outcome., Conclusion: Both pro-inflammatory and anti-inflammatory cytokines constitute a double-edged sword in sepsis; on one hand they are critical to eliminate the infection while on the other, excessive production can cause tissue and organ damage. Increase in cytokines such as IL-6, Il-8, IL-10, IL-18 and TNF-α may have implications in diagnosis and treatment of sepsis.
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- 2013
62. Characterization of T-cell memory phenotype after in vitro expansion of tumor-infiltrating lymphocytes from melanoma patients.
- Author
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Zhou J, Nagarkatti P, Zhong Y, and Nagarkatti M
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- CD28 Antigens biosynthesis, CD3 Complex biosynthesis, CD8-Positive T-Lymphocytes immunology, Cell Separation, Flow Cytometry, Humans, Hyaluronan Receptors biosynthesis, In Vitro Techniques, Interleukin-2 metabolism, L-Selectin biosynthesis, RNA, Small Interfering metabolism, Tumor Necrosis Factor Receptor Superfamily, Member 7 biosynthesis, Gene Expression Regulation, Neoplastic, Immunologic Memory, Lymphocytes, Tumor-Infiltrating immunology, Melanoma immunology, T-Lymphocytes immunology
- Abstract
Memory T-cell populations in human antitumor tumor-infiltrating lymphocytes (TILs) for adoptive cell transfer have not been fully characterized. Our studies demonstrated that CD62L, CD27 and CD28 positive effector memory T-cells were present in the TIL samples from the tumor tissues of melanoma patients and T-cell expansion led to the significant loss of memory T-cells. CD27- and CD28-positive T-cells had high levels of CD44 expression. T-Cell expansion resulted in significant down-regulation of CD44 expression. Interleukin-2 (IL-2) and anti-CD3 antibody stimulation may be responsible for CD44 down-regulation on CD8(+) T-cells during expansion. Furthermore, CD44 down-regulation using small interfering RNA (siRNA) on TILs dramatically reduced interferon-gamma and IL-2 release upon tumor stimulation. These results suggest that the regulation of CD44 expression in TILs may play an important role in memory T-cell maintenance and antitumor immune response.
- Published
- 2011
63. Unique SNP in CD44 intron 1 and its role in breast cancer development.
- Author
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Zhou J, Nagarkatti PS, Zhong Y, Creek K, Zhang J, and Nagarkatti M
- Subjects
- Adult, Black or African American, Aged, Aged, 80 and over, Base Sequence, Breast Neoplasms ethnology, Breast Neoplasms pathology, Female, Humans, Introns, Middle Aged, Molecular Sequence Data, Polymorphism, Single Nucleotide, Tumor Burden, White People, Young Adult, Breast Neoplasms genetics, Hyaluronan Receptors genetics
- Abstract
In the current study, we investigated if CD44 polymorphisms are associated with increased susceptibility to breast cancer. Direct nucleotide sequencing analysis identified a novel and unique single nucleotide polymorphism (SNP, designated as CD44 Ex2+14 A>G) in the CD44 intron 1 region in 84% of breast cancer patients, which was significantly higher than that seen in normal donors. Moreover, the breast cancer patients with homozygous unique SNP in CD44 intron 1 had breast cancer at earlier ages, larger tumor burden, more regional lymph node metastases at the time of diagnosis, and higher cancer recurrence rate. There was a strong association between the unique SNP in CD44 intron 1 and CD44 expression on peripheral blood mononuclear cells. Our results suggest that CD44 polymorphism is associated with breast cancer development, and CD44 polymorphism analysis may be effectively used in the risk assessment, prediction, prevention, diagnosis and genetic epidemiological analysis of breast cancer.
- Published
- 2010
64. Breast cancer immunotherapy.
- Author
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Zhou J and Zhong Y
- Subjects
- Adoptive Transfer, Animals, Antineoplastic Agents therapeutic use, Breast Neoplasms prevention & control, Cancer Vaccines, Clinical Trials as Topic, Female, Humans, Immunotherapy, Adoptive, Receptors, Antigen, T-Cell genetics, Receptors, Antigen, T-Cell metabolism, T-Lymphocytes cytology, T-Lymphocytes physiology, Tumor Cells, Cultured, Breast Neoplasms therapy, Immunotherapy
- Abstract
Breast cancer is a leading cause of cancer-related deaths in women worldwide. Although tumorectomy, radiotherapy, chemotherapy and hormone replacement therapy have been used for the treatment of breast cancer, there is no effective therapy for patients with invasive and metastatic breast cancer. Immunotherapy may be proved effective in treating patients with advanced breast cancer. Breast cancer immunotherapy includes antibody based immunotherapy, cancer vaccine immunotherapy, adoptive T cell transfer immunotherapy and T cell receptor gene transfer immunotherapy. Antibody based immunotherapy such as the monoclonal antibody against HER-2/neu (trastuzumab) is successfully used in the treatment of breast cancer patients with over-expressed HER-2/neu, however, HER-2/neu is over-expressed only in 25-30% of breast cancer patients. Cancer vaccine immunotherapy is a promising method to treat cancer patients. Cancer vaccines can be used to induce specific anti-tumor immunity in breast cancer patients, but cannot induce objective tumor regression. Adoptive T cell transfer immunotherapy is an effective method in the treatment of melanoma patients. Recent advances in anti-tumor T cell generation ex vivo and limited clinical trial data have made the feasibility of adoptive T cell transfer immunotherapy in the treatment of breast cancer patients. T cell receptor gene transfer can redirect the specificity of T cells. Chimeric receptor, scFv(anti-HER-2/neu)/zeta receptor, was successfully used to redirect cytotoxic T lymphocyte hybridoma cells to obtain anti-HER-2/neu positive tumor cells, suggesting the feasibility of treatment of breast cancer patients with T cell receptor gene transfer immunotherapy. Clinical trials will approve that immunotherapy is an effective method to cure breast cancer disease in the near future.
- Published
- 2004
65. Identification of a mutated fibronectin as a tumor antigen recognized by CD4+ T cells: its role in extracellular matrix formation and tumor metastasis.
- Author
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Wang HY, Zhou J, Zhu K, Riker AI, Marincola FM, and Wang RF
- Subjects
- Antigens, Neoplasm genetics, Base Sequence, Blotting, Western, Cell Movement, Fibronectins genetics, HLA-DR2 Antigen immunology, Humans, Melanoma immunology, Melanoma pathology, Molecular Sequence Data, RNA, Messenger genetics, RNA, Messenger metabolism, Tumor Cells, Cultured, Antigens, Neoplasm immunology, Antigens, Neoplasm metabolism, CD4-Positive T-Lymphocytes immunology, Extracellular Matrix metabolism, Fibronectins immunology, Fibronectins metabolism, Mutation, Neoplasm Metastasis
- Abstract
CD4+ T cells play an important role in orchestrating host immune responses against cancer, particularly by providing critical help for priming and extending the survival of CD8+ T cells. However, relatively little is known about major histocompatibility complex class II-restricted human tumor antigens capable of activating CD4+ T cells. Here, we describe the identification of a mutated fibronectin (FN) as a tumor antigen recognized by human histocompatibility leukocyte antigen-DR2-restricted CD4+ T cells. Deoxyribonucleic acid (DNA) sequencing analysis indicated that this gene contains a mutation that results in the substitution of lysine for glutamic acid and gives rise to a new T cell epitope recognized by CD4+ T cells. Tumor cells harboring the mutant FN resulted in the loss of FN matrix formation and the gain of metastatic potential based on the migration pattern compared with that of tumor cells that express wild-type FN. Additional experiments using cell lines stably expressing the mutated FN cDNA demonstrated that the point mutation in FN was responsible for the loss of FN staining in extracellular matrices and the enhancement of tumor cell migration. These findings represent the first demonstration that a mutated gene product recognized by CD4+ T cells is directly involved in tumor metastasis, which indicates the importance of CD4+ T cells in controlling the spread of tumor cells to distant anatomic sites.
- Published
- 2002
- Full Text
- View/download PDF
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