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Background: As indigestible carbohydrates, milk oligosaccharides possess various benefits for newborns, mainly through intestinal microbiota, among which 2'‐fucosyllactose (2'‐FL) is the most predominant milk oligosaccharide. However, knowledge about the fermentative characteristics of 2'‐FL in the gut remains limited, especially in the small intestine. The aim of this study is to explore the differential fermentability of 2'‐FL by the small and large intestinal microbiota of piglets using fructo‐oligosaccharide (FOS) and lactose as controls in an in vitro batch fermentation experiment. During fermentation, microbial composition was characterized along with gas production and short‐chain fatty acid production. Results: 2'‐Fucosyllactose showed differential fermentability in jejunal and colonic fermentation. Compared with the colon, 2'‐FL produced less gas in the jejunum than in the FOS and lactose groups (P < 0.05). Meanwhile, 2'‐FL exhibited a different influence on the microbial composition and metabolism in the jejunum and colon compared with FOS and lactose. In the jejunum, compared with the FOS and lactose groups, the 2'‐FL group showed a higher abundance of Bacteroides, Prevotella, and Blautia, but a lower abundance of Streptococcus and Lactobacillus (P < 0.05), with a higher level of propionate and a lower level of lactate during fermentation (P < 0.05). In the colon, compared with the FOS and lactose groups, 2'‐FL increased the abundance of Blautia, Faecalibacterium, and Lachnospiraceae FCS020, but decreased the abundance of Prevotella_9, Succinivibrio, and Megasphaera (P < 0.05) with an increase in acetate production (P < 0.05). Conclusion: Overall, the results suggested that the small intestinal microbiota had the potential to ferment milk oligosaccharides. Meanwhile, in comparison with FOS and lactose, 2′‐FL selectively stimulated the growth of propionate‐producing bacteria in the jejunum and acetate‐producing bacteria in the colon. These results demonstrated the differences in fermentation properties of 2'‐FL by small and large intestinal microbiota and provided new evidence for the application of 2'‐FL in optimizing gut microbiota. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The present study investigated the development of intestinal lactic acid bacteria (LAB) in piglets by high-throughput sequencing. Fresh feces from newborn piglets were collected at the ages of 1, 2, 3, 7, 14, 21, 24, 35 and 42 days. Sequencing of DNA amplicons of bacteria in feces amplified with LAB-specific primers showed that Lactobacillus was the predominant genus with an abundance higher than 70%, but tended to decrease after weaning (p = 0.059). The abundance of Streptococcus was the second predominant genus on day 1, then decreased significantly (p < 0.05). The relative abundance of Facklamia, Bacillus and Aerosphaera increased significantly after weaning (p < 0.05). At the species level, Lactobacillus reuteri, Uncultured Lactobacillus sp. and Lactobacillus mucosae dominated on day 3. Lactobacillus reuteri was the predominant bacteria at 7, 21, 24 and 35 days of age, and Lactobacillus sp. MWBPC 1-3-1 dominated on day 42. The relative abundance of Lactobacillus vaginalis, Lactobacillus johnsonii and Lactobacillus gasseri on day 24 was significantly higher than other ages (p < 0.05). These results indicate that the structure and predominant species of LAB changed dramatically from birth to weaning, which is of great significance to explore the LAB for the health of piglets. [ABSTRACT FROM AUTHOR]