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Copyright of Revista Foco (Interdisciplinary Studies Journal) is the property of Revista Foco and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Neurological conditions constitute approximately 10% of feline cases referred to veterinary clinics. Such cases often present manifestations of central nervous system (CNS) damage, including inflammatory lesions, neoplastic growth and structural and cellular transformations. Objectives: This study aimed at identifying histopathological changes in the CNS of cats that had succumbed to neurological symptoms. Methods: Microscopic evaluation of different sections within the CNS was conducted on 20 cats that had either died naturally or were euthanized due to neurological signs. After performing a necropsy, we examined the CNS tissues and conducted PCR testing to screen for possible viral infections, including feline infectious peritonitis (FIP), feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). Results: The majority of cases showed characteristic histopathologic lesions, notably mononuclear and suppurative meningoencephalitis, perivascular cuffing, disseminated intravascular coagulation (DIC), granulomatous meningoencephalitis, acute neuronal necrosis, liquefactive necrosis, epididymitis, thrombosis and demyelination. Among the 20 cats studied, 12 tested positive for FIP by PCR analysis, while 5 cats exhibited positive results for FIV. Also, FeLV PCR tests displayed positive results in 4 cats. No evidence of feline spongiform encephalopathy (FSE) was observed. Conclusion: This study is the first of its kind conducted in Iran. Based on the results, the most prevalent viral agents infecting cats' CNS were FIP, FIV and FeLV. The investigation revealed no evidence of FSE in cats with neurological signs. [ABSTRACT FROM AUTHOR]

The objective of this study was to establish an evaluation technique for the feline pelvic cavity using computed tomography (CT). Client-owned cats with a normal pelvis (n = 50) or pelvic stenosis (n = 3) were included. We categorized pelvic CT images in the normal pelvis group into two types according to pelvic cavity shape, then calculated the sacral index (SI), pelvic canal area (PCA), and PCA/sacral width index (PSI). The reference intervals of these variables were determined based on the results in the normal pelvis group. In the normal pelvis group, the PSI, unlike the SI and PCA, was not affected by body weight. In addition to high intraobserver and interobserver reproducibilities, the PSI showed no significant differences according to pelvic cavity shape. However, the SI exhibited significant differences among pelvic cavity shapes. In the stenosis group, the postoperative PSI in all cats was within the reference interval. However, the postoperative SI in one case was lower than the reference interval. The PSI may become a new method for evaluation of the pelvic cavity, including the pelvic floor. [ABSTRACT FROM AUTHOR]

Pimobendan is not currently approved for use in cats, although its usefulness in feline hypertrophic cardiomyopathy has been suggested. Reports indicate an increase in arrhythmic events following oral administration to healthy cats. Given the greater potency of intravenous administration compared to oral intake, it is conceivable that the incidence of arrhythmias may be increased following pimobendan injection. Therefore, this study aimed to investigate the proarrhythmic effects of pimobendan injection in cats. Five clinically healthy cats underwent physical examination, echocardiography, blood pressure measurements, and 24-hour Holter electrocardiography immediately before and after receiving pimobendan as an intravenous bolus dose of 0.15 mg/kg twice daily for 3 days. Additionally, a 24-hour Holter electrocardiography recording was conducted on the third day of pimobendan or placebo IV administration to assess heart rate, arrhythmias, and heart rate variability. Following pimobendan administration, there was a significant increase in total 24-hour heart rate. Echocardiography revealed a significant increase in mitral valve annulus systolic velocity (S') on the ventricular septal wall side, indicative of enhanced contractility. Only one cat exhibited paroxysmal ventricular tachycardia and an increase in the frequency of arrhythmic events. Conversely, in the remaining cats, a decreasing trend in the number of arrhythmias was observed. These findings indicate that intravenous administration of pimobendan may not be implicated in the onset of arrhythmias. Nevertheless, further research is warranted to explore the effects of intravenous pimobendan administration in cats with myocardial disease. [ABSTRACT FROM AUTHOR]

This study evaluated the antibacterial efficacy of 700 feline-derived lactic acid bacteria (LAB) strains against enteropathogenic Escherichia coli (EPEC), a common cause of diarrhea in cats. Following comprehensive screening, strains ZY25 and ZY35 were identified as the most effective, with inhibition zones of ≥22 mm. These strains demonstrated strong tolerance against stress conditions, such as low pH, bile salts, and gastrointestinal fluids, alongside high hydrophobicity and auto-aggregation abilities. Safety evaluations confirmed the absence of hemolytic activity, virulence factors, and antibiotic resistance genes. The antibacterial activity of these strains is attributed to the production of organic acids, particularly lactic acid and acetic acid. These findings suggest that strains ZY25 and ZY35 have potential as natural and effective probiotic treatments for managing EPEC-induced diarrhea in cats, thus offering an alternative to conventional antibiotics. [ABSTRACT FROM AUTHOR]

Abstract Background Limited information is available on electrolyte abnormalities in cats with chronic inflammatory enteropathy (CIE). Hypothesis/Objectives Report the prevalence of electrolyte abnormalities in cats with CIE compared to other gastrointestinal disorders, and determine their association with disease and outcome variables in cats with CIE. Animals Three hundred twenty‐eight client‐owned cats from 2 referral hospitals: CIE (132), alimentary small cell lymphoma (29), acute gastroenteritis (48), and healthy controls (119). Methods Retrospective study comparing serum electrolyte concentrations at time of diagnosis among the 4 groups of cats, and associations with clinical signs, intestinal mucosal fibrosis scores, treatment subclassification and outcome in CIE. Results Cats with CIE had lower sodium and higher potassium concentrations and lower sodium: potassium ratios compared with healthy cats (P

Abstract Background Monitoring serum phosphorus concentrations is crucial in the management of chronic kidney disease in cats. The diurnal variation of serum phosphorus concentration may affect clinical assessment. Hypothesis/Objectives Characterize the diurnal variation of serum phosphorus concentration in cats and determine the associations between changes in serum phosphorus concentration and several metabolites of phosphorus metabolism. Animals Six apparently healthy, intact male, specific‐pathogen‐free cats were housed in a photoperiod, humidity, and temperature‐controlled facility. Methods Blood sampling was performed hourly for 24 hours to obtain the serum concentrations of phosphorus, ionized calcium, parathyroid hormone, and calcidiol. Results were analyzed using linear mixed‐effect models to determine the significance of diurnal variation and associations between serum phosphorus concentrations and other metabolites over time. Results Diurnal variation in serum phosphorus concentration was noted with an apex around 11:00 followed by gradually declining concentrations to reach the nadir around 23:00. The serum phosphorus concentration again increased through the early morning on the next day. An approximately 25% difference in serum phosphorus concentration at the apex and the nadir was documented. A non‐linear relationship between the serum concentrations of phosphorus and ionized calcium over time was identified. Conclusions and Clinical Importance Diurnal variations of serum phosphorus concentration and associations between the trending of serum phosphorus and ionized calcium concentrations were evident in a group of clinically healthy adult cats housed in a controlled environment. These findings can help in the interpretation of clinical and research data regarding calcium and phosphorus metabolism and kidney health in cats.

Abstract Background Gabapentin is often administered PO for preappointment or in‐hospital anxiolysis in cats. A previous study reported mild changes on the neurologic examination after administration. Objectives Investigate the effects of gabapentin on anxiety, sedation, compliance, and neurologic examination in 2 age groups of cats. Animals Thirty‐one young cats and 12 geriatric cats perceived by their owners to be healthy and neurologically normal. Methods Prospective double‐blinded clinical crossover study. Assessment of baseline sedation and anxiety was performed before initial neurologic examinations and after gabapentin administration (100 mg/cat). Assessments were repeated 90 to 120 minutes after administration. Ease of handling pregabapentin and postgabapentin was assessed in the younger cats. All examinations were performed by a board‐certified veterinary neurologist and scoring of examinations was performed by a different, masked board‐certified neurologist. Results Sixteen cats (50%) in the younger cohort and 6 cats (50%) in the geriatric cohort exhibited an increase in their overall neurologic examination score postgabapentin administration, mainly through new or progressive postural reaction deficits and gait changes. Anxiety and sedation scores were significantly changed in the geriatric population (P < .01, P = .004, respectively); however, only sedation scores were significantly increased in the younger cats after gabapentin administration (P = .004). Conclusions and Clinical Importance All study participants showed mild neurologic changes after gabapentin administration, most markedly noted in the geriatric population. Dose reduction of gabapentin for preappointment anxiolysis and neurologic examination in geriatric patients should be considered.

Abstract Background The proportion of cats with hypertrophic cardiomyopathy (HCM) that lose systolic anterior motion of the mitral valve (SAM) in the long term is unknown. Hypothesis/Objectives Cats with HCM will lose SAM in the long term. Loss of SAM will be associated with greater age, longer scan‐interval, and altered left ventricular (LV) dimensions. Animals Sixty unsedated cats with HCM, not receiving beta blockers or pimobendan. Methods A retrospective cohort study from 2 referral centers. Cats were eligible if they had been diagnosed with HCM and had a repeat echocardiogram ≥1 year later. Clinical and echocardiographic data of the left heart variables were collected. Results Thirty‐eight cats had SAM at the initial scan. After a median follow‐up time of 2.1 years (range: 1.0‐5.9), 7 cats had lost SAM (18%) and 5 cats (23%) gained SAM. On follow‐up, cats with SAM at the initial scan had a larger left atrium (P = .037), lower left atrial fractional shortening (P = .014), greater LV internal diameter in end‐systole (P = .002), and lower LV fractional shortening (P

Abstract Background Colonic stenosis is a rare postoperative complication of ovariohysterectomy in cats, leading to dyschezia and fecal diameter reduction. In cats, while there are reports of colonic stenosis after midline approach ovariohysterectomy, there are no specific reports of flank approach ovariohysterectomy. Case presentation This report describes a severe case of a one-year-old British shorthair female cat presenting with gastrointestinal signs, including dyschezia and reduced fecal diameter, three weeks after flank approach ovariohysterectomy. Despite abdominal radiography, proctography with barium sulfate, colonoscopy, CT, and hematological analysis, the cause of colonic stenosis remained unclear. During exploratory laparotomy, an annular tissue band was found encircling the descending colon, resulting in severe local stenosis. After excision of the tissue band, the presenting clinical signs of the cat were rapidly improved. This result suggests that colonic stenosis caused by tissue band should be considered when diagnosing postoperative complications in flank approach ovariohysterectomy in cats. Conclusion Colon stenosis due to annular tissue band restriction should be considered one of the differentials for postoperative complications in flank approach ovariohysterectomy in cats.

OBJECTIVE: To screen modulators of biogenic amine (BA) neurotransmission for the ability to cause fentanyl to decrease isoflurane minimum alveolar concentration (MAC) in cats, and to test whether fentanyl plus a combination of modulators decreases isoflurane MAC more than fentanyl alone. STUDY DESIGN: Prospective, experimental study. ANIMALS: A total of six adult male Domestic Short Hair cats. METHODS: Each cat was anesthetized in three phases with a 1 week washout between studies. In phase 1, anesthesia was induced and maintained with isoflurane, and MAC was measured in duplicate using a tail clamp stimulus and standard bracketing technique. A 21 ng mL-1 fentanyl target-controlled infusion was then administered and MAC measured again. In phase 2, a single cat was administered a single BA modulator (buspirone, haloperidol, dexmedetomidine, pregabalin, ramelteon or trazodone) in a pilot drug screen, and isoflurane MAC was measured before and after fentanyl administration. In phase 3, isoflurane MAC was measured before and after fentanyl administration in cats co-administered trazodone and dexmedetomidine, the two BA modulator drugs associated with fentanyl MAC-sparing in the screen. Isoflurane MAC-sparing by fentanyl alone, trazodone-dexmedetomidine and trazodone-dexmedetomidine-fentanyl was evaluated using paired t tests with p < 0.05 denoting significant effects. RESULTS: The MAC of isoflurane was 1.87% ± 0.09 and was not significantly affected by fentanyl administration (p = 0.09). In the BA screen, cats administered trazodone or dexmedetomidine exhibited 26% and 22% fentanyl MAC-sparing, respectively. Trazodone-dexmedetomidine co-administration decreased isoflurane MAC to 1.50% ± 0.14 (p < 0.001), and the addition of fentanyl further decreased MAC to 0.95% ± 0.16 (p < 0.001). CONCLUSIONS AND CLINICAL RELEVANCE: Fentanyl alone does not affect isoflurane MAC in cats, but co-administration of trazodone and dexmedetomidine causes fentanyl to significantly decrease isoflurane requirement.

Surgical castration is a primary method for controlling male fertility, but it is impractical for large-scale population control of stray animals. Developing nanoparticle-mediated sterilants that induce cell apoptosis rather than necrosis is a complex and promising area of research. This study aimed to investigate the impact of intratesticular administration of alpha-mangostin encapsulated in nanostructured lipid carriers (AM-NLC) on testicular changes and any associated adverse effects over a 168-day observation period. Thirty-two healthy mature tomcats were enrolled. None of the cats treated with either AM-NLC (n = 28) or blank NLC (n = 4) exhibited noticeable complications related to pain or stress throughout the study, as assessed by clinical examination, blood profiles, and serum amyloid A levels. Histopathological analysis of AM-NLC treated cats revealed seminiferous epithelium degeneration, leading to defective tubules. Key findings included germ cell depletion, disorganized spermatogenic cells without spermatids in certain areas, apoptotic bodies, and intracytoplasmic vacuolization. The intertubular compartment showed no signs of inflammation, hyalinization, fibrosis, or necrosis. Despite widespread degeneration, some normal tubules were present in focal areas. The severity score of seminiferous tubule degeneration significantly increased from day 56 onwards (P < 0.05), suggesting a gradual and progressive compromise of the seminiferous epithelium. In contrast, testes from the blank-NLC group exhibited normal spermatogenesis. Overall, there were no significant changes in the volume of dissected testes, serum testosterone levels, or apoptotic index in AM-NLC-treated cats (P > 0.05). In conclusion, this study represents the first in vivo investigation of apoptotic-inducing agents as a novel nanomedicine-based antifertility compound for non-surgical castration in male animals. While the AM-NLC formulation proved safe for intratesticular administration, it failed to induce infertility in cats, as epididymal spermatozoa persisted throughout the study. Further research into alternative apoptosis-inducing nanomedicine sterilants remains both essential and challenging. [ABSTRACT FROM AUTHOR]

Semen collection in cats in the clinic setting can be difficult. However, semen analysis is vital when evaluating breeding soundness of a male. Electroejaculation (EEJ) is currently the most reliable semen collection method but requires specialized equipment and training of the operator. Chemical ejaculation followed by urethral catheterization (UC) is a technique that allows semen collection without special equipment: a catheter is placed into the urethra of a sedated tom and semen is collected passively into the catheter. Earlier studies used the sedative medetomidine at high doses for this procedure. However, medetomidine has been replaced with dexmedetomidine in some countries. This study sought to compare the results of EEJ and UC for semen collection in the domestic cat using dexmedetomidine, a potent α2-adrenoceptor agonist (α2A), as a substitute for medetomidine at the equivalent dose to that used in earlier studies. Twelve domestic cats were collected thrice at weekly intervals. All cats received intramuscular ketamine (5 mg/kg) and intramuscular dexmedetomidine (30 μg/kg) for initial cleanout via EEJ, then randomly underwent either EEJ or UC one week apart. The EEJ was performed under the same anesthetic protocol as the initial cleanout. The UC was performed using intramuscular dexmedetomidine at a dose of 60 μg/kg. Success of collection, total sperm number, sperm morphology, and motility characteristics were analyzed. Sperm was collected successfully from all 12 cats via EEJ and from 11/12 via UC. There were no significant differences in the percentage of total motile, progressively motile, or morphologically normal sperm between ejaculate types when averaged across all cats or individual cats. Although UC yielded a lower volume and higher concentration ejaculate, it consistently produced a lower total sperm number than ejaculates retrieved via EEJ (17.91 x 106 total sperm for UC versus 46.51 x 106 total sperm for EEJ). These results indicated that dexmedetomidine is a very effective sedative and performed satisfactorily in both procedures at the doses used in this study. It was also safe with no adverse effects on healthy toms. EEJ remained the most reliable in terms of assessing semen quality and retrieving semen with adequate number of sperm for breeding purposes. However, UC with dexmedetomidine at this dose demonstrated a 92 % success rate, presenting itself as a remarkably consistent alternative. • 50 mcg/kg dexmedetomidine produces a representative sperm sample in cats. • Chemical and electroejaculation yield comparable ejaculate concentration in cats. • Chemical and electroejaculation motilities and morphology are comparable in cats. • Electroejaculation yields higher volume and sperm number than chemical ejaculation. • Nucleocounter and hemocytometer concentration measurements are equivalent. [ABSTRACT FROM AUTHOR]

Abstract Background This study updates the knowledge of current canine and feline endoparasitic prevalence in Finland. The previous studies reported intestinal worm prevalence of 5.9% in dogs and 7.1% in cats. We also determined the anthelmintic regime and background data of dogs and cats concerning Toxocara spp. infection. Altogether 664 canine and 379 feline (including 46 shelter cats’) fecal samples from over six-month-old animals were examined with quantitative Mini-FLOTAC method using zinc sulfate with a specific gravity of 1.35. Of these samples, 396 canine and 89 feline samples were analyzed using the Baermann method for nematode larvae. A fenbendazole efficacy study was conducted with 12 animals positive for Toxocara spp. Results Endoparasites were found in the feces of 3.5% of dogs, 3.6% of pet cats, and 41.3% of shelter cats. The most common findings in dogs were strongylid (1.7%) and Toxocara canis (0.9%) eggs. Trematode (0.4%), Eucoleus spp. (0.3%), taeniid (0.2%), and Trichuris vulpis (0.2%) eggs, and Cystoisospora spp. oocysts (0.2%) were also detected. One dog (0.2%) was positive for Crenosoma vulpis based on the Baermann method. Toxocara cati (3.3%), taeniid (0.6%), and trematode (0.3%) eggs were found in pet cats’ samples. The findings in shelter cat samples were T. cati (34.8%), Eucoleus spp. (13.0%), Cystoisospora spp. oocysts (10.9%), taeniids (8.7%), and Toxoplasma gondii/Hammondia hammondii oocysts (2.2%). Fenbendazole efficacy was adequate in all treated animals, except one cat. The background data revealed 31.2% of dogs being dewormed less than once a year or never. Under twelve-month-old dogs and dogs that were dewormed twice a year were most likely to be T. canis- infected. Shelter cats, male cats, mixed-breed cats, cats that were dewormed two to four times a year, and cats with a history of parasitic infections were most likely to be T. cati infected. Conclusions The prevalence of pet canine and feline intestinal parasites in Finland is low, particularly the Toxocara spp. prevalence. In free-roaming cats Eucoleus spp. is surprisingly prevalent. The parasite control strategies reported do not follow the ESCCAP guidelines. Typically, owners deworm their pets only once a year or less frequently.

Abstract Urothelial carcinoma (UC) occurs uncommonly in cats and no association has previously been observed with long‐term indwelling urinary implants. An 18‐year‐old male castrated domestic shorthair cat initially was presented for hematuria, leading to the diagnosis of a right‐sided ureterolithiasis and severe pyelectasia on ultrasound examination, prompting right‐sided subcutaneous ureteral bypass (SUB) device placement. The cat subsequently had intermittent hematuria and dysuria, without ultrasonographic abnormality of the bladder or positive urine culture. Thirteen months later the patient developed refractory lower urinary tract signs, azotemia, a proliferative mass in the region of the cystostomy tube component of the SUB device and evidence of left ureteral obstruction. Cystostomy tube revision and left‐sided SUB device placement were performed, as well as a partial cystectomy for removal of the mass. Upon histopathology, the mass was diagnosed as a UC. To our knowledge, UC associated with a long‐term indwelling cystostomy catheter component of a SUB device has not been reported in veterinary medicine.

Background and Aim: Insulin resistance and type 2 diabetes mellitus are common health issues in obese (OB) cats. In humans, obesity leads to alterations in adipokine and proinflammatory cytokine secretion, causing persistent inflammation. The inflammatory impact of obesity in cats remains unproven. This study investigated associations between obesity and inflammatory and metabolic changes in three groups of client-owned Brazilian domestic shorthair cats: naturally lean, overweight (OW), and OB. Materials and Methods: Cats from the Veterinary Hospital of Professor Sylvio Barbosa e Cardoso (FAVET/UECE) were clinically evaluated. Blood samples were collected for hematological and biochemical profile measurements, and part of the serum was used for measuring adipokine and inflammatory cytokines using sandwich enzyme-linked immunosorbent assay. Results: In both the OW and OB groups, serum cholesterol and insulin concentrations increased, while triglyceride concentrations were notably elevated in the OB group. In the OW and OB groups, serum adiponectin, tumor necrosis factor-α, and interleukin-1β levels were elevated, and leptin levels were significantly higher in the OB group. Conclusion: This study is the first in Brazil to reveal increased serum levels of inflammatory markers in OW and OB client-owned felines. OW cats exhibited higher proinflammatory marker levels, implying obesity-induced inflammation.

Abstract Background Although regular health screening is recommended, long‐term follow‐up data in healthy aged cats are lacking. Objectives Determine the most common conditions in a large group of apparently healthy older cats and which diseases are manifested within 2 years in cats confirmed to be healthy based on extensive health screening. Animals Client‐owned cats. Methods Prospective study. Thorough history, physical examination, blood tests, and urinalysis were performed in 259 apparently healthy mature adult (7‐10 years) and senior (>10 years) cats. Semi‐annual follow‐up examinations were performed in 201 confirmed healthy cats. Results At baseline, 21% of apparently healthy cats were not considered healthy but were diagnosed with International Renal Interest Society (IRIS) ≥ stage 2 chronic kidney disease (CKD; 7.7%) or hyperthyroidism (4.6%), among other disorders. Disease occurred significantly more frequently in senior cats compared with mature adult cats. In addition, 40% cats were overweight, 35% had moderate to severe dental disease, and 22% had abnormal cardiac auscultation findings. Within 2 years, 28% of mature adult and 54% of senior cats that were confirmed healthy at inclusion developed new diseases, most commonly IRIS ≥ stage 2 CKD (cumulative incidence, 13.4%), hyperthyroidism (8.5%), chronic enteropathy, hepatopathy or pancreatitis (7.5%), or neoplasia (7%). Conclusions and Clinical Importance The high prevalence and 2‐year incidence of physical examination abnormalities and systemic diseases in apparently healthy older cats argue for regular health screening in cats ≥7 years of age. Although more common in senior cats, occult disease also occurs in mature adult cats, and owners should be informed accordingly.

Abstract Background Hypercoagulability has been documented in cats with cardiac disease. However, hemostatic parameters, including viscoelastic coagulation monitoring (VCM) have not been reported in cats with arterial thromboembolism (ATE). Hypothesis/Objectives Compare VCM parameters in cats with acute cardiogenic ATE and in control cats. Animals Sixteen cats with ATE and 30 control cats. Methods Multicenter university‐based prospective study. Cardiogenic ATE was diagnosed based on physical examination and by ultrasonographically‐diagnosed left atrial enlargement. Viscoelastic coagulation monitor analysis, CBC, serum biochemistry profile and coagulation profile were performed at admission in cats with ATE. Analysis from healthy control cats was performed using blood collected by direct venipuncture. Our objective was comparison of VCM parameters clot time (CT), clot formation time (CFT), alpha angle (Angle), maximum clot formation (MCF), amplitude at 10 and 20 minutes (A10 and A20, respectively) and clot lysis index at 30 and 45 minutes (LI30 and LI45, respectively) between ATE and control cats. Results Cats with ATE had a decreased angle compared to control cats, with a median (range) of 43° (30‐48°) compared to 47° (14‐59°; P = .01). The parameters A10, A20 and MCF were decreased in ATE cats compared to control cats with a median (range) of 19 units (8–32) compared to 22 units (6–38), 24.5 units (11‐40) compared to 29 units (10‐47) and 29.5 units (13‐44) compared to 33.5 units (14‐53), respectively (P = .01, .01 and .01, respectively). The parameters CT, CFT, LI30 and LI45 were similar between groups (P = .22, .09, .62 and .34, respectively). Conclusions and Clinical Importance Cats with cardiogenic ATE cats have VCM parameters consistent with hypocoagulability compared with healthy cats.

Background: This study updates the knowledge of current canine and feline endoparasitic prevalence in Finland. The previous studies reported intestinal worm prevalence of 5.9% in dogs and 7.1% in cats. We also determined the anthelmintic regime and background data of dogs and cats concerning Toxocara spp. infection. Altogether 664 canine and 379 feline (including 46 shelter cats') fecal samples from over six-month-old animals were examined with quantitative Mini-FLOTAC method using zinc sulfate with a specific gravity of 1.35. Of these samples, 396 canine and 89 feline samples were analyzed using the Baermann method for nematode larvae. A fenbendazole efficacy study was conducted with 12 animals positive for Toxocara spp. Results: Endoparasites were found in the feces of 3.5% of dogs, 3.6% of pet cats, and 41.3% of shelter cats. The most common findings in dogs were strongylid (1.7%) and Toxocara canis (0.9%) eggs. Trematode (0.4%), Eucoleus spp. (0.3%), taeniid (0.2%), and Trichuris vulpis (0.2%) eggs, and Cystoisospora spp. oocysts (0.2%) were also detected. One dog (0.2%) was positive for Crenosoma vulpis based on the Baermann method. Toxocara cati (3.3%), taeniid (0.6%), and trematode (0.3%) eggs were found in pet cats' samples. The findings in shelter cat samples were T. cati (34.8%), Eucoleus spp. (13.0%), Cystoisospora spp. oocysts (10.9%), taeniids (8.7%), and Toxoplasma gondii/Hammondia hammondii oocysts (2.2%). Fenbendazole efficacy was adequate in all treated animals, except one cat. The background data revealed 31.2% of dogs being dewormed less than once a year or never. Under twelve-month-old dogs and dogs that were dewormed twice a year were most likely to be T. canis- infected. Shelter cats, male cats, mixed-breed cats, cats that were dewormed two to four times a year, and cats with a history of parasitic infections were most likely to be T. cati infected. Conclusions: The prevalence of pet canine and feline intestinal parasites in Finland is low, particularly the Toxocara spp. prevalence. In free-roaming cats Eucoleus spp. is surprisingly prevalent. The parasite control strategies reported do not follow the ESCCAP guidelines. Typically, owners deworm their pets only once a year or less frequently. [ABSTRACT FROM AUTHOR]

Simple Summary: A variation in the Alström syndrome protein 1 (ALMS1) gene was recently identified as a possible cause of hypertrophic cardiomyopathy (HCM) in Sphynx cats. The primary aims of this study were to describe the prevalence of HCM in Sphynx cats in New Zealand, and to assess the association between HCM and ALMS1 gene. In this prospective study, 55 apparently healthy Sphynx cats from registered Sphynx breeders and pet owners in New Zealand were screened by a cardiologist. A total of 42 of these cats had a repeat cardiac examination after median 1.8 years (range: 1.6–2.2). The frequency of ALMS1 variant was 70.9%. At the median age of 5.8 years (range: 2.4–13.1), the prevalence of HCM was 40% (20 out of 55 cats). Three cats with HCM died during the study with congestive heart failure. Necropsy revealed that all three cats suffered a heart attack (myocardial ischemia or infarction). The ALMS1 gene was not associated with the HCM diagnosis. In summary, HCM is commonly diagnosed in this population, suggesting Sphynx cats are predisposed to this disease. The ALMS1 variant was also frequently detected; however, it was not associated with the HCM diagnosis in the studied population. Recently, hypertrophic cardiomyopathy (HCM) in Sphynx cats has been associated with a variant in the gene encoding Alström syndrome protein 1 (ALMS1). The primary aims of this study were to describe the prevalence of HCM in Sphynx cats in New Zealand, and to assess the association between HCM and the ALMS1 variant in this population. In this prospective study, 55 apparently healthy Sphynx cats from registered Sphynx breeders and pet owners in New Zealand were screened by a cardiologist. A total of 42 of these cats had a repeat cardiac examination after median 1.8 years (range: 1.6–2.2). The frequency of the ALMS1 variant was 70.9% (11 homozygous and 28 heterozygous). At the median age of 5.8 years (range: 2.4–13.1), the prevalence of HCM was 40% (20 out of 55 cats). Three cats with HCM died during the study with congestive heart failure. All three cats had focal but extensive myocardial ischemia or infarction at necropsy. The ALMS1 variant was not associated with the HCM diagnosis. In summary, HCM was common in the studied cohort, suggesting Sphynx cats are predisposed to this disease. While the ALMS1 variant was also frequently detected, it was not associated with HCM in this population. [ABSTRACT FROM AUTHOR]

There is a complex interplay between viral infection and host innate immune response regarding disease severity and outcomes. Neutrophil hyperactivation, including excessive release of neutrophil extracellular traps (NETs), is linked to exacerbated disease in acute COVID-19, notably in hospitalized patients. Delineating protective versus detrimental neutrophil responses is essential to developing targeted COVID-19 therapies and relies on high-quality translational animal models. In this study, we utilize a previously established feline model for COVID-19 to investigate neutrophil dysfunction in which experimentally infected cats develop clinical disease that mimics acute COVID-19. Specific pathogen-free cats were inoculated with SARS-CoV-2 (B.1.617.2; Delta variant) (n = 24) or vehicle (n = 6). Plasma, bronchoalveolar lavage fluid, and lung tissues were collected at various time points over 12 days post-inoculation. Systematic and temporal evaluation of the kinetics of neutrophil activation was conducted by measuring markers of activation including myeloperoxidase (MPO), neutrophil elastase (NE), and citrullinated histone H3 (citH3) in SARS-CoV-2-infected cats at 4 and 12 days post-inoculation (dpi) and compared to vehicle-inoculated controls. Cytokine profiling supported elevated innate inflammatory responses with specific upregulation of neutrophil activation and NET formation-related markers, namely IL-8, IL-18, CXCL1, and SDF-1, in infected cats. An increase in MPO-DNA complexes and cell-free dsDNA in infected cats compared to vehicle-inoculated was noted and supported by histopathologic severity in respiratory tissues. Immunofluorescence analyses further supported correlation of NET markers with tissue damage, especially 4 dpi. Differential gene expression analyses indicated an upregulation of genes associated with innate immune and neutrophil activation pathways. Transcripts involved in activation and NETosis pathways were upregulated by 4 dpi and downregulated by 12 dpi, suggesting peak activation of neutrophils and NET-associated markers in the early acute stages of infection. Correlation analyses conducted between NET-specific markers and clinical scores as well as histopathologic scores support association between neutrophil activation and disease severity during SARS-CoV-2 infection in this model. Overall, this study emphasizes the effect of neutrophil activation and NET release in SARS-CoV-2 infection in a feline model, prompting further investigation into therapeutic strategies aimed at mitigating excessive innate inflammatory responses in COVID-19. [ABSTRACT FROM AUTHOR]

The increased LH levels resulting from the absence of negative feedback after castration has been linked to long-term health issues. A need exists for an alternative contraceptive agent that functions without interfering the LH pathways. This study aimed to develop antibody fragments against the follicular-stimulating hormone receptor (anti-FSHr) using phage-display technology and evaluate its effects on Sertoli cell functions. Phage clones against the extracellular domain of dog and cat FSHr selected from an antibody fragment phagemid library were analyzed for binding kinetics by surface plasmon resonance. Sertoli cells were isolated from testes of adult animals (five dogs and five cats). Efficacy test was performed by treating Sertoli cell cultures (SCCs) with anti-FSHr antibody fragments compared with untreated in triplicates. Expressions of androgen binding protein (ABP), inhibin subunit beta B (IHBB) and vascular endothelial growth factor A (VEGFA) mRNA in SCCs were quantified by RT-qPCR. The results demonstrated that the molecular weight of the purified dog and cat anti-FSHr antibody fragment was 25 kDa and 15 kDa, respectively. Based on protein molecular weight, the antibody fragment of dogs and cats was therefore, so-called single-chain variable fragments (scFv) and nanobody (nb), respectively. The binding affinity with dissociation constant (K D) was 2.32 × 10−7 M and 2.83 × 10−9 M for dog and cat anti-FSHr antibody fragments, respectively. The cross-binding kinetic interactions between the dog anti-FSHr scFv and the cat ECD of FSHr could not be fitted to the curves to determine the binding kinetics. However, the cross-binding affinity K D between the cat anti-FSHr nb and the dog ECD FSHr was 1.75 × 10−4 M. The mRNA expression of ABP , IHBB and VEGFA in SCCs was less (P < 0.05) in both dogs (12.26, 4.07 and 5.11 folds, respectively) and cats (39.53, 14.07 and 20.29 folds, respectively) treated with anti-FSHr antibody fragments, indicating the Sertoli cell functions were suppressed. In conclusion, this study demonstrated the establishment of species-specific antibody fragments against FSHr in SCCs for dogs and cats. The fragment proteins illustrate potential to be developed as non-surgical contraceptive agent targeting FSHr in companion animals. • The anti-follicular stimulating hormone receptor (anti-FSHr) antibody fragment was developed in dogs and cats by biopanning. • The anti-FSHr antibody fragments demonstrated inhibitory function in the Sertoli cell cultures. • The anti-FSHr antibody fragments demonstrated species-spefic binding. [ABSTRACT FROM AUTHOR]

Introduction: This retrospective study highlights the salient aspects of a series of feline patients affected with bisphosphonate related osteonecrosis of the jaw. Though more commonly published in human literature, this presentation is rare in cats. The authors hope that this study will assist in making this a more globally known entity with subsequent improved prognosis. Methods: Data was retrospectively obtained from the medical records between 2015 and 2021 of 20 cats with Medication Related Osteonecrosis of the Jaw. Data included patient information, clinical history, presenting complaint, systemic diseases, details referable to hypercalcemia and treatment thereof, bisphosphonate specifics (dose and duration), clinical presentation of the lesion, diagnostic testing including radiographic and histopathologic descriptions, treatment, and outcome. Results: Pertinent results include that all 20 cats who developed Medication Related Osteonecrosis of the Jaw had been treated for idiopathic hypercalcemia with the bisphosphonate medication alendronate. Eighty-five percent of the cases had prior dental extractions at the site of MRONJ lesion. Ninety-five percent of the affected cats required a surgical procedure to control the disease. Thirty-five percent of cases required at least one revision surgery after the initial procedure was performed. Diagnosis of MRONJ was made by a correlation of diagnostic findings and patient history. No single diagnostic, or combination was pathognomonic for lesion diagnosis. As well, there were no statistically significant associations between patient variables assessed and the overall patient outcome. Discussion: The case series reveals that cats with feline idiopathic hypercalcemia treated with alendronate may be at a risk for development of MRONJ, a serious oral condition with significant morbidity. Prior dental extraction sites in patients concurrently treated with bisphosphonate medications were often associated with MRONJ lesions. Therefore, any needed dental surgery should be performed prior to the use of bisphosphonates where possible. The authors have also included a relevant comparative literature review. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the morphological and morphometric features, as well as the histological composition of the medial and lateral menisci of domestic cats. A total of 180 menisci were obtained from 45 skeletally mature cat cadavers, followed by measuring the peripheral extension, width and thickness, circumference of the body and the body with horns, articulating height, and superior articulating length. A histological assessment was conducted to determine the meniscal cellularity and histomorphology. Each meniscus was evaluated in three regions (cranial horn, body, and caudal horn) and three zones (outer, middle, and deep). The proteoglycan content and collagen distribution were also examined. Overall, 62.2% of the medial meniscus displayed a crescentic shape, whereas 86.7% of the lateral meniscus exhibited a C-shaped configuration. The morphometric analysis revealed that the lateral menisci significantly differed (P<0.05) in circumference, thickness, width, and articulating height from the medial menisci. Despite cell density was not significantly different across the regions, the cellularity decreased from the outer zone to the deep zone of the menisci. Proteoglycan content was not significantly different in the various regions but was increased significantly (P<0.05) in the middle meniscal zone. Types I, II, and III collagens were distributed throughout the meniscal regions and zones. Type I collagen was abundant within the meniscus. In contrast, type III collagen is primarily localized in the middle zone. In conclusion, feline menisci exhibit morphometric variations and distinctive histological features, offering crucial insights for veterinarians and researchers. [ABSTRACT FROM AUTHOR]

Objective: To investigate the utility of the neutrophil‐to‐lymphocyte ratio (NLR) as a prognostic marker in cats with blunt trauma. Design: Retrospective study from January 2018 to December 2021. Setting: University teaching hospital. Animals: Medical records of 177 cats admitted with blunt trauma were evaluated. History, clinical findings, blood cell count‐based ratios, thoracic radiographs on presentation, and outcome were reviewed. The Animal Trauma Triage (ATT) score was assessed in each cat and classified as mild (1–3), moderate (4–7), and severe trauma (≥8). Forty‐eight healthy blood donor cats served as the control population. NLR, neutrophil counts, and lymphocyte counts were compared between cats with blunt trauma and controls, and among trauma patients. Interventions: None Measurements and Main Results: NLR, neutrophil counts, and lymphocyte counts significantly differ in cats with blunt trauma compared to controls (p < 0.001), and NLR was higher in patients with thoracic trauma (p = 0.044). Nonsurvivors had lower lymphocyte counts (p = 0.041), although those values do not appear to be clinically relevant. A significant increase in NLR was observed with increasing severity of trauma (p < 0.001). The NLR was not associated with the length of hospitalization, intensive care assistance, or mortality. Conclusion: NLR is a widely available diagnostic tool, which can be used in addition to ATT to assess trauma severity, although in our study it was not predictive of the outcome. [ABSTRACT FROM AUTHOR]

Objective: To report a case of delayed death after initial successful treatment of severe bromethalin intoxication in a cat. Case Summary: A 2‐year‐old neutered male domestic shorthair cat presented as a referral for bromethalin toxicosis and progressive neurological decline. At the time of referral, the cat was nonambulatory tetraparetic with minimal motor function in all 4 limbs and exhibited a dull mentation. Within the first 4 hours of hospitalization, the cat's neurological status continued to rapidly decline, and osmotherapy, corticosteroids, and intralipids were administered. The treatment regimen also included levetiracetam, thiamine, cholestyramine, vitamin E, and gingko biloba. Six days after bromethalin ingestion, the cat displayed marked neurological improvement with near normal mentation and mild tetraparesis and was discharged. The cat continued to do well at home with improving neurological status and function. Nine days after discharge, the cat was presented dead on arrival. Postmortem examination revealed transforaminal brain herniation secondary to spongy degeneration of the white matter and increased intracranial pressure as the cause of death. New Information Provided: To the authors' knowledge, this is the first report of a cat that suffered delayed death despite initial neurological improvement after being treated for severe bromethalin toxicosis. [ABSTRACT FROM AUTHOR]

The "Arrow pin technique" is a modified version of using intramedullary pins to mend femoral diaphyseal/metaphyseal (supracondylar) fractures. The arrow pins were "custom made" by cutting locally available stainless-steel rod into predetermined lengths pins, further flattening one end, creating groves on either side of the flattened end with a file to give the appearance of an arrowhead, and shaping the opposite end as a trocar point. The pin insertion was accomplished aseptically through lateral approach on the distal femur, through the trochlear groove under general anaesthesia in cats. The clinical efficacy of this arrow pin technique was evaluated in six cats. All the cats (n=6) were client owned and < 1-year-old, with supracondylar distal femoral fractures. Following the clinical and radiological examinations, the type and location of the fracture, details of the fixation method applied, postoperative clinical and radiological results were assessed. After radiographic evaluation, long bone fractures were classified as supracondylar fractures of type I overridden closed fractures. Following the insertion of an intramedullary arrow pin, robust stability was accomplished, as evidenced radiographically. The patients were able to bear weight on the affected limb in 3-5 days and began walking normally on day 14 after surgery. The study found that the single "custom made" arrow pins provided adequate stability and resistance to rotational and axial forces in distal femoral fractures, and it confirmed that it would provide a significant fracture reduction in long bone fractures in young cats at a low cost. [ABSTRACT FROM AUTHOR]

Urothelial carcinoma (UC) occurs uncommonly in cats and no association has previously been observed with long‐term indwelling urinary implants. An 18‐year‐old male castrated domestic shorthair cat initially was presented for hematuria, leading to the diagnosis of a right‐sided ureterolithiasis and severe pyelectasia on ultrasound examination, prompting right‐sided subcutaneous ureteral bypass (SUB) device placement. The cat subsequently had intermittent hematuria and dysuria, without ultrasonographic abnormality of the bladder or positive urine culture. Thirteen months later the patient developed refractory lower urinary tract signs, azotemia, a proliferative mass in the region of the cystostomy tube component of the SUB device and evidence of left ureteral obstruction. Cystostomy tube revision and left‐sided SUB device placement were performed, as well as a partial cystectomy for removal of the mass. Upon histopathology, the mass was diagnosed as a UC. To our knowledge, UC associated with a long‐term indwelling cystostomy catheter component of a SUB device has not been reported in veterinary medicine. [ABSTRACT FROM AUTHOR]

Bone mineral density (BMD) varies with aging and both systemic and local diseases; however, such evidence is lacking in feline medicine. This may be due to the need for general anesthesia in cats for direct BMD measurements using dual-energy X-ray absorptiometry (DXA) or quantitative computed tomography (QCT). In this study, computed digital absorptiometry (CDA), an indirect relative BMD-measuring method, was optimized to select an X-ray tube setting for the quantitative assessment of the feline knee joint. The knee joints of nine cats were radiographically imaged and processed using the CDA method with an aluminum density standard and five X-ray tube settings (from 50 to 80 kV; between 1.2 and 12 mAs). The reference attenuation of the X-ray beam for ten steps (S1–S10) of the density standard was recorded in Hounsfield units (HU), compared between X-ray tube settings, and used to determine the ranges of relative density applied for radiograph decomposition. The relative density decreased (p < 0.0001) with an increase in kV and dispersed with an increase in mAs. Then, the percentage of color pixels (%color pixels), representing ranges of relative density, was compared among S1–S10 and used for the recognition of background artifacts. The %color pixels was the highest for low steps and the lowest for high steps (p < 0.0001), regardless of X-ray tube settings. The X-ray tube setting was considered the most beneficial when it effectively covered the lowest possible HU ranges without inducing background artifacts. In conclusion, for further clinical application of the CDA method for quantitative research on knee joint OA in cats, 60 kV and 1.2 mAs settings are recommended. [ABSTRACT FROM AUTHOR]

Simple Summary: To date, there are very few studies of the bony nasal cavity of the domestic cat and even fewer of big felids. A detailed and exhaustive anatomical study of this cavity in the domestic cat is presented in this paper through dissections, trepanations and anatomical sections. This work has allowed to delve deeper into the anatomy of the bony nasal cavity. This study deepens the knowledge of this region through diagnostic imaging techniques, such as computed tomography, three-dimensional reconstruction in maximum-intensity projection and volume rendering, that allow for the spatial visualization of this cavity. These reconstructed images represent an improvement in the anatomical study of the cavity and will help veterinary clinicians in the diagnosis of nasal pathologies. Obtaining molds by three-dimensional printing allows for acquiring models very close to reality that capture these structures in detail. These techniques, which in human medicine have already led to many clinical and therapeutic improvements, are a very useful tool in veterinary medicine that will lead to advances and improvements in the diagnosis and treatment of nasal pathologies, improving the survival of many threatened species. This study aims to develop three-dimensional printing models of the bony nasal cavity and paranasal sinuses of big and domestic cats using reconstructed computed tomographic images. This work included an exhaustive study of the osseous nasal anatomy of the domestic cat carried out through dissections, bone trepanations and sectional anatomy. With the use of OsiriX viewer, the DICOM images were postprocessed to obtaining maximum-intensity projection and volume-rendering reconstructions, which allowed for the visualization of the nasal cavity structures and the paranasal sinuses, providing an improvement in the future anatomical studies and diagnosis of pathologies. DICOM images were also processed with AMIRA software to obtain three-dimensional images using semiautomatic segmentation application. These images were then exported using 3D Slicer software for three-dimensional printing. Molds were printed with the Stratasys 3D printer. In human medicine, three-dimensional printing is already of great importance in the clinical field; however, it has not yet been implemented in veterinary medicine and is a technique that will, in the future, in addition to facilitating the anatomical study and diagnosis of diseases, allow for the development of implants that will improve the treatment of pathologies and the survival of big felids. [ABSTRACT FROM AUTHOR]

Data on the age of apical closure in felines is limited. Moreover, differences in age of apical closure between male and female cats have not been studied. The aim of this study was to determine the timing of apical closure in cats and determine if sex or position in the dental arch affected closure. In this retrospective descriptive study, intraoral radiographs were obtained at monthly or multiple-monthly intervals for 18 cats. Nine were intact females and 9 were neutered males, ranging from 6 to 9.4 months of age at the start of the study which ranged over an 8-month period. Radiographs were evaluated to establish age of apical closure for all canine teeth as well as the mandibular premolar and molar teeth. Mandibular canine tooth apices closed between 10 and 12 months of age and maxillary canine tooth apices closed between 12 and 14 months of age. The mesial and distal root apices of both the mandibular third and fourth premolar teeth closed between 8 and 9 months. The mandibular first molar tooth mesial and distal root apices closed between 8 and 8.5 months. Root apices of canine teeth closed earlier in female cats than in male cats with mandibular canine tooth root apices closing significantly earlier than maxillary canine tooth roots in both sexes. These findings suggest that there are notable differences in age of apical closure between male and female cats and discernible trends in timing of apical closure among teeth in the dental arcade. [ABSTRACT FROM AUTHOR]

Trazodone hydrochloride is a tetracyclic antidepressant commonly used in human, gaining popularity in feline patients as a pre-appointment anxiolytic and sedative medication. However, the oral administration route in cats poses difficulties for owners and limits practicality. This study evaluated the efficacy and safety of transdermal trazodone in cats. At the first visit, the cat stress score (CSS), global sedation score (GSS), behavioral response score (BRS), and owner-assessed overall experience score (OES) were used to observe and record the behavior during transportation and examination. Thereafter, each cat was given 150 mg of transdermal trazodone before the second visit. The respiratory rate, heart rate, pulse rate, systolic blood pressure, blood test, adverse effects, and response time were compared between the two visits. Thirteen cats were enrolled in this study. Transdermal trazodone significantly lowered CSS of outward trip (P = 0.005) and return trip (P = 0.005) (Wilcoxon signed-rank test). No significant differences were observed in CSS and GSS during the examination. Moreover, the BRS was significantly low (P = 0.018), but the significance varied depending on the examination procedure and behavior. The OES questionnaire showed a significant reduction in stress during the outward trip (P = 0.002), return trip (P = 0.005), and examination (P = 0.003) (all Wilcoxin signed ranks test). Physiologic parameters and laboratory values were not significantly affected, and the adverse effects were few and mild. In conclusion, transdermal trazodone was well tolerated at a single dose of 150 mg/cat. It can reduce stress and anxiety before a veterinary visit, resulting in a better patient-owner experience. • Transdermal trazodone was well tolerated at a single dose of 150 mg/cat. • Transdermal trazodone significantly lower stress and anxiety during transportation. • Most owners give positive feedback to this method of trazodone administration. • Transdermal trazodone results in a better patient-owner experience. [ABSTRACT FROM AUTHOR]

There are no published reports describing the anatomic variations of the phrenicoabdominal (PhAbd) veins in cats. The aim of this study was to evaluate the anatomic variations of the PhAbd veins in cats without adrenal disease. This is an anatomic study. Abdominal CT images of cats acquired from January 2021 to February 2023 were retrospectively reviewed. Inclusion criteria were the presence of pre‐ and postcontrast CT images of the abdomen and the absence of any abdominal diseases that compromise the left and right PhAbd veins visualization. A total of 128 cats were included. Three different vascular anatomic variations were found in the distal pericaval segment of the left phrenicoabdominal (LPhAbd) vein. In the first type, found in 65 cases (50.8%), the LPhAbd vein drained directly into the caudal vena cava. In the second type defined IIa, found in 25 cases (19.5%), the LPhAbd vein drained into the distal third of the left renal vein, less than 5 mm from its opening into the caudal vena cava. In the third type defined IIb, found in 38 cases (29.7%), the LPhAbd vein drained into the distal third of the left renal vein, more than 5 mm from its opening into the caudal vena cava. The right phrenicoabdominal vein had a consistent path and drained into the caudal vena cava in all cases. The knowledge of these vascular variations is expected to help the surgeon during adrenalectomy due to masses with vascular invasion in cats. [ABSTRACT FROM AUTHOR]

A 12‐year‐old domestic shorthair cat was presented with relapsing noncardiogenic chylothorax. CT‐lymphangiography of the thorax confirmed bilateral pleural effusion without evidence of an underlying intra‐thoracic origin. Abdominal CT‐lymphangiography revealed a 2.6 cm diameter splenic mass surrounded by chylous effusion actively collecting during ultrasonographic assessment. Following splenectomy, histopathological analysis revealed that the splenic mass exhibited characteristics indicative of splenic angiosarcoma. This case report highlights the utility of advanced thoracic and abdominal imaging, notably CT‐lymphangiography, in the diagnostic evaluation of chylothorax in cats. The identification of a splenic mass encased in chylous effusion should prompt a proactive case management strategy. [ABSTRACT FROM AUTHOR]

A 5‐year‐old intact male mixed‐breed cat weighing 4.5 kg was referred to our hospital with a left testicular mass. CT revealed mild heterogeneous contrast enhancement and calcification in the testicular mass. A well‐defined, contrast‐enhancing, multiloculated mass with fluid‐filled areas was extended from the testicular mass in the scrotum to the caudal aspect of the left kidney. The abdominal mass extended to the right crus of the diaphragm, and the gastrointestinal tract was compressed dorsally. Histopathology was consistent with teratoma. Characteristic CT findings in a feline testicular teratoma may include calcification and cystic areas. [ABSTRACT FROM AUTHOR]

In felines, ocular and nonocular melanomas are uncommon tumors that represent a diagnostic challenge for pathologists, especially when amelanotic. To date, the immunohistochemical diagnostic panel in cats is based on specific melanocytic markers (Melan-A and PNL2) and a nonspecific but sensitive marker (S100). In human medicine, SOX-10 is reported to be a sensitive antibody for the detection of melanoma micrometastasis in the lymph node. TRP-1, an enzyme involved in melanogenesis, has recently been used in humans and dogs as a specific melanocyte marker. The aim of this study was to evaluate the cross-reactivity and the expression of SOX-10 and TRP-1 antibodies in feline normal tissue and melanocytic tumors. Thirty-one cases of ocular, cutaneous, and oral melanomas were retrospectively evaluated and confirmed by histopathological examination and by immunolabeling with Melan-A and/or PNL2. SOX-10 nuclear expression in normal tissues was localized in epidermal, subepidermal, hair bulb, and iridal stromal melanocytes and dermal nerves. In melanomas, nuclear expression of SOX-10 was detected in ocular (11/12; 92%), oral (6/7; 86%), and cutaneous sites (12/12; 100%). TRP-1 cytoplasmic immunolabeling in normal tissue was observed in epidermal and bulbar melanocytes and in the lining pigmented epithelium of the iris and in its stroma. Its expression was positively correlated to the degree of pigmentation in the tumor and was observed in 75% of ocular (9/12), 43% of oral (3/7), and 33% of cutaneous melanomas (4/12). This study demonstrated the cross-reactivity of SOX-10 and TRP-1 antibodies in feline non-neoplastic melanocytes and their expression in ocular and nonocular melanomas. [ABSTRACT FROM AUTHOR]

Three cats, aged 2 to 11 years, presented to the University of Minnesota Veterinary Diagnostic Laboratory over a 3-year period following euthanasia or death due to respiratory distress. Thoracic radiographs revealed nodular, soft tissue opacities throughout the lung fields in all cases. On postmortem examination, approximately 60% to 80% of the lung parenchyma were expanded by multifocal to coalescing, well-demarcated, beige, semi-firm nodules. Histologically, large numbers of neutrophils, fewer macrophages, fibrin, and cellular and karyorrhectic debris effaced the pulmonary parenchyma. The inflammatory foci contained aggregates of gram-negative cocci. 16s rRNA Sanger sequencing and whole-genome sequencing identified the bacteria isolated from the lung of all cats under aerobic conditions as a novel Neisseria spp. Based on whole-genome sequence analysis, all 3 sequences shared 92.71% and 92.67% average nucleotide identity with closely related Neisseria animaloris NZ LR134440T and Neisseria animaloris GCA 002108605T, respectively. The in silico DNA-DNA hybridization identity compared to our isolates was 46.6% and 33.8% with strain DSM Neisseria zoodegmatis 21642 and strain DSM 21643, respectively. All 3 sequences have less than 95% average nucleotide identity and less than 70% DNA-DNA hybridization identity, suggesting that the 3 isolates are a novel species of the genus Neisseria. Infection with Neisseria spp. induces an embolic pneumonia in cats that radiographically and pathologically resembles a metastatic neoplastic process and should be considered among the etiologic differential diagnoses in cases of infectious pulmonary disease with a disseminated, nodular lung pattern. [ABSTRACT FROM AUTHOR]

Simple Summary: In this retrospective study, three dogs and three cats were selected from the database of the Chonnam National University Teaching Hospital from February 2017 to June 2019. The inclusion criteria for the selection of patients were as follows: (1) clinical data including age, sex, breed, clinical signs, histology, fine-needle aspiration, or fluid centesis were available and (2) intraluminal CEUS was performed; CEUS findings helped confirm the diagnosis. Each patient underwent physical examination, blood tests, radiography, and conventional ultrasonography. Intraluminal CEUS was performed using contrast agents, including SonoVue, agitated saline, saline, or a combination for GI hydrosonography in two patients and sono-cystourethrography in four patients. GI hydrosonography could assess the anatomic relationship between the mass and gastric lumen after administration of a mixture of 0.1 mL SonoVue and 30 mL/kg water in case 1 and could dilate the colonic lumen to detect the thickened wall using saline infusion in case 2. Upon sono-cystourethrography, communication between the urinary tract and prostatic cyst was clearly visualized in case 3, and narrowing of the urethral lumen secondary to prostatomegaly could be ruled out in case 4 with pyogranulomatous prostitis using agitated saline. The saline or agitated saline is appropriate for filling the lumen and improving the acoustic window for GI hydrosonography. Intraluminal CEUS examinations helped to assess the patency of the lumen, evaluate the extent of luminal dilation, rule out luminal narrowing, determine the presence of a mass within the lumen, and identify rupture sites. Administering intraluminal fluid can improve the acoustic window for the visualization of the lumen and wall layers in the cavitary organs. Microbubbles in ultrasound contrast agents can also be used for intracavitary applications to enhance visualization of the lesion in human patients. However, there was no literature extending the clinical application of intraluminal contrast-enhanced ultrasonography (CEUS) to patients with naturally occurring diseases in veterinary medicine. This case series aims to describe the detailed application and diagnostic value of intraluminal CEUS in six clinical cases with naturally occurring gastrointestinal (GI) and urinary tract diseases. [ABSTRACT FROM AUTHOR]

Simple Summary: Melanocytic tumors (MTs) are more prevalent in dogs than in cats. In dogs, they are the most frequent malignancy of the oral cavity, whereas in cats, ocular melanomas predominate. This study provides epidemiologic data (2005–2024) on MTs for both dogs and cats. Totals of 21128 canine and 4808 feline tumors were analyzed by the Veterinary Pathology Service of the Department of Veterinary Medicine (University of Perugia). Among these, 845 canine MTs (329 melanocytomas; 512 melanomas) were diagnosed, 485 from the skin, 193 from the oral mucosa, and 104 from the mucocutaneous junction. Older dogs were more likely to develop melanomas than melanocytomas (p < 0.001). In contrast, among feline tumors, only 60 were melanocytic (6 melanocytomas, 53 melanomas). Of these, 29 were cutaneous, 18 were ocular, and 9 were oral. In dogs, melanomas were more common in mucocutaneous locations than in cutaneous locations (p < 0.05); moreover, they were more common in the oral cavity compared to all other sites (p < 0.001). In cats, ocular melanomas were more common than cutaneous ones (p < 0.05). This study provides the prevalence of MTs in dogs and cats, supporting distinct epidemiological patterns of MTs, and confirming the significance of species-specific differences in the tumor prevalence, localization, and age distribution. MTs are prevalent in dogs, representing the most frequent oral malignancy, compared to cats, in which ocular melanomas predominate. This study investigates the canine and feline MT epidemiology (2005–2024) of cases submitted to the Veterinary Pathology Service (University of Perugia). Among the canine neoplasms, 845 (4%) were melanocytic: 329 (39%) melanocytomas; 512 (61%) melanomas. Of these, 485 (57%) were cutaneous (4% of canine cutaneous neoplasms), 193 (23%) were oral (50% of oral canine neoplasms), and 104 (12%) were mucocutaneous. The average age of affected dogs was 10 years. Older dogs were more likely to have melanomas compared to melanocytomas (p < 0.001). There were 60 (1%) feline MTs: 6 (10%) melanocytomas; 53 (88%) melanomas. Of these, 29 (48%) were cutaneous (1% of feline cutaneous tumors), 18 (30%) were ocular, and 9 (15%) were oral (22% of feline oral tumors). The average age of affected cats was 11 years. In dogs, mucocutaneous melanomas were more common compared to cutaneous ones (p < 0.05); oral melanomas were more common compared to all other sites (p < 0.001). In cats, ocular melanomas were more common compared to cutaneous ones (p < 0.05). Our study provides the MT prevalence in a selected canine and feline population, revealing MT epidemiological patterns, highlighting species-specific differences in the tumor prevalence, localization, and age distribution. [ABSTRACT FROM AUTHOR]

A 3‐year‐old male neutered domestic shorthair cat presented with lethargy, hyporexia, and pyrexia of unknown origin. Biochemical analysis using a Beckman Coulter AU480 demonstrated marked increases in creatine kinase and aspartate aminotransferase, indicative of severe muscle injury, with concurrent presumptive myoglobinuria on urinalysis. A marked, non‐physiologic increase in measured bicarbonate and resultant negative anion gap was documented; however, calculated bicarbonate obtained via a point‐of‐care blood gas analyzer was within normal limits. Laboratory error due to interference by lactate dehydrogenase was suspected and supported by the results of subsequent biochemical testing. Artifactual increases in bicarbonate have been documented in cases of rhabdomyolysis in horses, cows, and a bird. However, to the best of the authors' knowledge, this is the first report to demonstrate this spurious change in a cat. [ABSTRACT FROM AUTHOR]

A feline cutaneous melanocytic tumor in a 1‐year‐old cat is reported. The cytologic, histologic, and immunohistochemical characteristics of the pediatric feline cutaneous melanocytoma are included. A solitary, black‐colored nodule on the head was histologically diagnosed as the epithelioid type of melanocytoma. Immunohistochemically, the neoplastic melanocytes demonstrated immunoreactivity to the S100 and MelanA antibodies but not to the PNL2 antibody. Despite high mitotic counts (17/10HPFs) and a Ki67 index (33%) suggesting malignant melanoma, the patient exhibited a favorable 5‐month outcome. The mitotic activity of the neoplastic cells may not provide a reliable prediction for feline melanocytic tumors. [ABSTRACT FROM AUTHOR]

Objective: Diabetes mellitus (DM) is a prevalent endocrine condition found in cats. Recent research has identified a connection between a higher risk of diabetes in cats and genetic factors. This genetic susceptibility to type 2 diabetes is associated with genes that control insulin secretion and function, as well as those that impact factors like obesity. The purpose of the study was to determine the prevalence of the melanocortin 4 receptor (MC4R) gene missense mutation among cats and to investigate the relationship between body condition score (BCS) and the occurrence of diabetes in felines. Materials and Methods: Genotype analysis was done for 45 samples. The research results were obtained with the polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) method. The 1,200-bp PCR product was cut with BstOI restriction enzyme. Results: Upon analyzing the data, it was found that 37.8% (n = 17) of the subjects carried the MC4R gene mutation. Across the entire population of cats studied, the C allele associated with the mutation was most prevalent in the group of cats with DM (frequency of 0.3) and least common among the clinically healthy cats (frequency of 0.2). Conclusion: After analysis of the study results, a statistically significant difference was observed between cats with DM and clinically healthy cats in the comparison of their BCS (p < 0.05). The association of the MC4R gene polymorphism with overweight and the development of diabetes has been identified. [ABSTRACT FROM AUTHOR]

The term biomarker encompasses various biological indicators that objectively reflect a patient's medical status with precision and reproducibility. These indicators range from basic measurements like pulse and blood pressure to more intricate laboratory tests. Cardiac markers are crucial for accurate and prompt diagnosis of heart diseases in animals. Given the challenge of diagnosing cardiac diseases in small animals due to nonspecific clinical signs, cardiac markers provide quantitative indicators of biological processes. These markers include cardiac troponins for myocardial injury, natriuretic peptides for myocardial function, lipoproteins for serum homeostasis, and markers for inflammation of the cardiovascular system. Among natriuretic peptides, atrial natriuretic peptide (ANP) has emerged as a significant tool in diagnosing and monitoring cardiac diseases. ANP, primarily synthesized in cardiac atria, regulates salt and fluid excretion, counteracts vasoconstriction, and inhibits the renin-angiotensin-aldosterone system, contributing to the maintenance of cardiovascular homeostasis. Additionally, it functions as a biomarker for ventricular hypertrophy and congestive heart failure (CHF) in animals. Furthermore, it protects against hypertension and cardiac remodeling by demonstrating antagonism to the same system. This review addresses the definition of biomarkers within the context of molecular biology, elucidates their multifaceted functions in the animal organism in light of integrative physiology, and explores the pathologies correlated with ANP, with an emphasis on its etiopathogenesis and clinical manifestations. [ABSTRACT FROM AUTHOR]

Objective Procedures Results Conclusions Investigate the tolerance, stability, and efficacy of topical 0.1% and 1% atropine in cats.Six cats underwent two trials separated by a 2‐week washout period. One drop of artificial tears was placed in one randomly selected eye (control), and one drop of either 0.1% atropine (Trial I) or 1% atropine (Trial II) was placed in the other eye. Immediate adverse effects were recorded for severity (0–3) and duration (seconds). Horizontal pupil diameter (HPD), pupillary light reflexes (PLRs), intraocular pressure (IOP), Schirmer tear test‐1 (STT‐1), and heart rate (HR) were monitored at baseline then 8 h post‐administration. PLRs were assessed for a total of 72 h. Stability was assessed weekly for 1 month in room temperature and refrigerated conditions, evaluating solution clarity, pH, and drug concentrations.Adverse effects had a significantly lower severity score and shorter duration with 0.1% versus 1% atropine (severity 1.2 ± 0.4 vs. 2.5 ± 0.5, p = .010; duration 107.5 ± 53.3 vs. 293.3 ± 106.5 s, p = .009). HPD was significantly greater than baseline measurements as early as 40 min for both atropine formulations. Pupils were non‐responsive for a significantly shorter duration with 0.1% versus 1% atropine (median 7 h vs. 47.5 h, p = .031). Compared with control eyes, IOP was significantly elevated by 1% atropine (p = .021) but not 0.1% atropine (p = .502). No significant differences were noted in STT‐1 and HR measurements. Both solutions were stable in room temperature and refrigerated conditions for 1 month.Diluted 0.1% atropine was stable and better tolerated by cats, offering a potential alternative to feline patients that experience adverse effects from topical 1% atropine. [ABSTRACT FROM AUTHOR]

Introduction Methods and Materials Results Conclusion Conjunctivitis is a prevalent feline ocular surface disorder, often accompanied by inflammation. Inflammatory cytokines, such as tumor necrosis factor‐α (TNF‐α) and interleukin‐6 (IL‐6), play a crucial role in the pathogenesis of conjunctival inflammation. This study aimed to evaluate the levels of TNF‐α and IL‐6 in the tears of cats with conjunctivitis and compare them with healthy controls, thereby enhancing our understanding of the inflammatory processes in feline conjunctivitis.Tear samples were collected from cats of various breeds diagnosed with conjunctivitis (n = 15) and healthy control cats (n = 5) using Schirmer strips. The levels of TNF‐α and IL‐6 were measured using the enzyme‐linked immunosorbent assay (ELISA) method. Protein concentration were measured using Bradford assay and data were expressed as pg/mg protein of tear sample.Our results revealed a statistically significant increase in the levels of both TNF‐α and IL‐6 in cats with conjunctivitis compared to the control group (p < .0001). Positive correlation were observed between tear IL‐6 (p < .001, r = 0.902) and TNF‐α (p < .001, r = 0.919) with clinical grades of conjunctivitis.The results demonstrated a significant elevation in the levels of TNF‐α and IL‐6 in the tears of cats with conjunctivitis, suggesting that these cytokines are involved in the inflammatory response of feline conjunctivitis. These findings could pave the way for new diagnostic and therapeutic approaches, focusing on cytokine modulation, to manage feline conjunctivitis more effectively. [ABSTRACT FROM AUTHOR]

Simple Summary: Stem cell therapy for veterinary patients such as cats often requires a significant quantity of mesenchymal stromal/stem cells (MSCs), typically expanded in laboratory conditions. However, this expansion process can alter the characteristics and genetic stability of these cells. This study focused on assessing the characteristics of MSCs derived from the adipose tissue of cats (cAT-MSCs) during culture conditions. For this purpose, cell morphological features, growth behavior, and cytogenetic stability were examined in passages 2, 4, and 6. Additionally, MSCs' multipotency and surface markers were assessed. The cAT-MSCs exhibited a spindle-shaped morphology, typical of MSCs. As the cells were cultured over successive passages, a reduction in their growth rate was observed after passage 4, accompanied by abnormalities in the nuclei. cAT-MSCs showed multipotency and their surface marker expression aligned with the expected immunophenotype of MSCs. Cytogenetic studies revealed some structural abnormalities in the chromosomes of the cAT-MSCs, such as gaps, breaks, deletions, duplications, and early chromatid segregation. Nevertheless, these alterations did not show a significant increase over subsequent passages. In conclusion, cAT-MSCs decreased their proliferative capacity after passage 4, accompanied by morphological alterations and signs of structural instability. Stem cell therapy in cat patients needs a high quantity of mesenchymal stromal/stem cells (MSCs) requiring in vitro propagation under culture conditions which may potentially impact cellular characteristics and genetic stability. This study aimed to assess the in vitro characteristics and cytogenetic stability of cat adipose tissue-derived MSCs (cAT-MSCs). For this purpose, morphological features, clonogenic potential, and proliferative capacity of cAT-MSCs were assessed at passages 2 (P2), P4, and P6. Multipotency and immunophenotype were evaluated. Cytogenetic analyses were conducted up to P6. The cAT-MSCs exhibited a spindle-shaped morphology in early passages. The doubling time increased from 2.5 days at P2 to 9.4 at P4 and 10.5 at P6, accompanied by the observation of nuclear abnormalities such as cluster formation, karyorrhexis, karyolysis, and a decline in the mitotic index at P4. Cells demonstrated multipotency capacity and were CD45−, CD90+, and CD44+. Metaphase analysis at P2 and P4 revealed some indications of structural instability such as gaps, breaks, deletions, duplications, and early chromatid segregation, but these alterations did not show an increase across passages. In conclusion, cAT-MSCs decreased their proliferative capacity after P4, accompanied by morphological alterations and signs of structural instability. [ABSTRACT FROM AUTHOR]

Simple Summary: Although Anaplasma (A.) phagocytophilum infection in cats is considered to be less frequent compared with dogs, there is evidence indicating that the risk of the infection in cats might be underestimated. The study aimed to find out if infections in cats are underestimated and to discover which factors increase the risk of infection. Blood samples of 1015 cats across Europe were tested for A. phagocytophilum DNA from 2017 to 2022. The number of samples sent for testing increased over the 6 years. Anaplasma phagocytophilum DNA was found in 76 out of 1015 cats (7.5%). Infections were more common in Northern Europe than in Central or Southern Europe. During summer, the number of positive samples was significantly higher compared with winter (p = 0.047). The risk for A. phagocytophilum infection in cats should not be underestimated, especially in Northern Europe. Preventing tick bites is essential for cats' health all over Europe, not just in the Mediterranean regions. Infections with Anaplasma (A.) phagocytophilum in cats seem to be rare. The study aimed to determine whether infections in cats are underestimated and to identify the risk factors for infection. Blood samples of 1015 cats across Europe (2017–2022), sent to IDEXX Laboratories, Germany, were tested for A. phagocytophilum DNA. The influence of the cats' origin on A. phagocytophilum infection was assessed by univariable analysis, while multivariable logistic regression evaluated associations with the cats' sex and age, and the years, and seasonality of the samples' submission. Furthermore, univariable linear regression was used to determine patterns in PCR orders. The number of submitted samples increased significantly during the 6 years (p = 0.042). Anaplasma phagocytophilum DNA was detected in 76/1015 of cats (7.5%, 95% CI 6.0–9.3%). Infections were significantly more common in Northern compared to Central (p < 0.001, OR: 8.70) and Southern Europe (p < 0.001, OR: 39.94). A significantly higher likelihood for infections during the summer compared with winter (p = 0.047, OR: 3.13) was found. Bacteremia with A. phagocytophilum in European cats is not uncommon. Anaplasma phagocytophilum infection should be considered an important risk, particularly in Northern Europe. Effective tick prevention is crucial for managing feline health across Europe, not just in the Mediterranean region. [ABSTRACT FROM AUTHOR]

A 9-year-old, neutered male, domestic short-haired cat was referred for recurrent ascites of unknown etiology over a week. Physical examination revealed abdominal distension and ultrasonography revealed a large volume of ascites throughout the abdominal cavity; this was interpreted as modified transudate. The mesentery and abdominal fat were hyperechoic and edematous. Fat tissue was assessed using fine-needle aspiration cytology, and adipocytes, fat-phagocytizing macrophages, and neutrophils were identified. Computed tomography revealed a pancreatic mass connected to the left pancreatic leg. Exploratory laparoscopy confirmed nodular masses and organ adhesions, leading to a tentative diagnosis of sclerosing encapsulating peritonitis. The cat was administered prednisolone, vitamin E, and tamoxifen but died 22 days after the initial therapy. Necropsy revealed a multi-lobulated pancreatic tumor (10 × 10 cm) tightly attached to the stomach and intestine, with a large amount of ascites. The peritoneum, stomach, intestine, and mesentery were covered with numerous disseminated nodules of various sizes (1-5 mm diameter). Microscopically, the tumor consisted of extensive adipose tissue, locally extensive inflammatory infiltrates, fibrous connective tissue, and small invasive proliferative glands. Well-defined small irregular glands composed of singlelayered epithelial cells that appear to be of ductal origin were surrounded by an abundant desmoplastic stroma. Neoplastic nodules were widespread in the liver, stomach, peritoneum, mesentery, mesenteric lymph nodes, lungs, and urinary bladder. Immunohistochemistry revealed that the neoplastic glands were positive for pan-cytokeratin, confirming the pancreatic epithelial origin of the tumor. This is the first report of sclerosing encapsulating peritonitis accompanied by aggressive pancreatic adenocarcinoma of presumed ductal origin and extensive metastasis in a cat. [ABSTRACT FROM AUTHOR]

A 5-year-old neutered female Korean domestic shorthair cat diagnosed with oral squamous cell carcinoma (SCC) presented to the hospital with severe oral purulent discharge, anorexia, and lethargy. Owing to extensive lesions, surgical excision and radiation therapy were not feasible. Instead, prior to metronomic therapy with toceranib, the patient received an intravenous injection of feline umbilical cord-derived mesenchymal stem cells (fUC-MSCs) (1 × 106 cells/10 mL of saline) to reduce inflammation. No acute side effects (such as fever, increased respiratory rate, diarrhea, and vomiting) were observed following stem cell therapy. For 6 days, purulent discharge, bleeding, swelling, a bad odor, and crust exfoliation in the tumor area on the face were dramatically reduced. However, the patient exhibited difficulty in voluntarily receiving foods, and weight loss persisted. Starting from the 7th day, purulent discharge, bleeding, and odor at the SCC area worsened again. Toceranib, low-dose NSAIDs (meloxicam, every other day), antibiotics (cefazoline), and gabapentin were administered; however, they were not effective in reducing the pus, bleeding, foul odor, and crust exfoliation at the SCC area. Symptoms of pain, weakness, and weight loss progressed, leading to the choice of euthanasia with the owner's consent approximately 1 month later. This case report reveals that allogeneic fUC-MSCs have a slight short-term effect on purulent discharge, bleeding, odor, and crust exfoliation and may be additional therapy for feline oral SCC. [ABSTRACT FROM AUTHOR]

Background and Aim: Insulin resistance and type 2 diabetes mellitus are common health issues in obese (OB) cats. In humans, obesity leads to alterations in adipokine and proinflammatory cytokine secretion, causing persistent inflammation. The inflammatory impact of obesity in cats remains unproven. This study investigated associations between obesity and inflammatory and metabolic changes in three groups of client-owned Brazilian domestic shorthair cats: naturally lean, overweight (OW), and OB. Materials and Methods: Cats from the Veterinary Hospital of Professor Sylvio Barbosa e Cardoso (FAVET/UECE) were clinically evaluated. Blood samples were collected for hematological and biochemical profile measurements, and part of the serum was used for measuring adipokine and inflammatory cytokines using sandwich enzyme-linked immunosorbent assay. Results: In both the OW and OB groups, serum cholesterol and insulin concentrations increased, while triglyceride concentrations were notably elevated in the OB group. In the OW and OB groups, serum adiponectin, tumor necrosis factor-α, and interleukin-1β levels were elevated, and leptin levels were significantly higher in the OB group. Conclusion: This study is the first in Brazil to reveal increased serum levels of inflammatory markers in OW and OB client-owned felines. OW cats exhibited higher proinflammatory marker levels, implying obesity-induced inflammation. [ABSTRACT FROM AUTHOR]