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Deinococcus radiodurans R1 exposed to a lethal dose of cadmium shows differential expression of a large number of genes, including frnE (drfrnE) and some of those involved in DNA repair and oxidative stress tolerance. The drfrnE::nptII mutant of D. radiodurans showed growth similar to that of the wild type, but its tolerance to 10 mM cadmium and 10 mM diamide decreased by ~15- and ~3-fold, respectively. These cells also showed nearly 6 times less resistance to gamma radiation at 12 kGy and ~2-fold-higher sensitivity to 40 mM hydrogen peroxide than the wild type. In trans expression of drFrnE increased cytotoxicity of dithiothreitol (DTT) in the dsbA mutant of Escherichia coil Recombinant drFrnE showed disulfide isomerase activity and could maintain insulin in its reduced form in the presence of DTT. While an equimolar ratio of wild-type protein could protect malate dehydrogenase completely from thermal denaturation at 42°C, the C22S mutant of drFrnE provided reduced protection to malate dehydrogenase from thermal inactivation. These results suggested that drFrnE is a protein disulfide isomerase in vitro and has a role in oxidative stress tolerance of D. radiodurans possibly by protecting the damaged cellular proteins from inactivation. [ABSTRACT FROM AUTHOR]

Demonstrates that low-density lipoprotein receptor-related protein 5 (LRP5) is required for normal cholesterol metabolism and glucose-induced insulin secretion. Comparison of plasma proteins in mice lacking LRP5; Absence of significant effects in the sole LPR5 deficiency; Mediation of both apolipoprotein E-dependent and apolipoprotein E-independent catabolism of plasma lipoproteins by LRP5.

Details the expression cloning and characterization of GPI-HBP1, a novel glycosylphosphatidylinositol-anchored high density lipoprotein (HDL)-binding protein. Amino acid sequence of GPI-HBP1; Visualization of receptor mediated uptake of fluorescent HDL by CHO cells expressing murine GPI-HBP1.